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Tylosin Production by Mutant Resistant to Oleic Acid  

Choi, Du-Bok (Biotechnology Laboratory, B-K Company Ltd.)
Choi, On-You (United Graduate School of Agricultural Science Shizuoka University)
Moon, Ok-Ran (Department of Environmental Engineering, Chosun University)
Yoon, Mi-Ran (Department of Environmental Engineering, Chosun University)
Ji, Sung-Nam (Department of Environmental Engineering, Chosun University)
Shin, Dae-Yewn (Department of Environmental Engineering, Chosun University)
Publication Information
Journal of Environmental Health Sciences / v.31, no.5, 2005 , pp. 360-364 More about this Journal
Abstract
When rapeseed oil as the carbon source was used for tylosin production from Streptomyces fradiae TP-1239 was very sensitive to oleic acid. Cell growth was restrained by adding 0.8 g/l of oleic acid to the culture broth. Mutant strain TM-224-1 resistant to 1.2 g/l of oleic acid was obtained by screening in solid and liquid media containing oleic acid. The uptake rate of oleic acid by TM-224-1 was approximately 3.8 fold higher than the parent strain. For comparing the TM-224-1 and the parent strain, batch cultures were carried out in a jar fermentor. Cell growth of TM-224-1 strain was higher than the parent strain after two days of culturing. However, after four days of culturing, it was similar to that of the parent strain. The amount of rapeseed oil consumed by TM-224-1 and the parent strain were 60.5 and 78.2 g/l, respectively. The production and yield of tylosin was aproximately 2.0 and 3.2 fold higher than the parent strain, respectively. From these results, it was concluded that this mutant, which was resistant to oleic acid, has improved tylosin production.
Keywords
타이로신;올레인산 내성균;유체유;
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