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http://dx.doi.org/10.15207/JKCS.2018.9.5.091

A study of Expression of TGF-β1, c-Myc, Erb-B2 and Thymosin-β4 Gene in Alcoholic Liver Damage Tissue.  

Kim, Jean-Soo (Department of Clinical Laboratory Science, Daejeon Health Institute of Technology)
Choi, Sang-Ki (Department of Biology, Sunchon National University)
Publication Information
Journal of the Korea Convergence Society / v.9, no.5, 2018 , pp. 91-97 More about this Journal
Abstract
This study has been conducted to see the expression of $TGF-{\beta}_1$, c-Myc, Erb-B2 and $Thymosin-{\beta}_4$ genes in ethanol - damaged liver tissues. Experimental groups were divided into 2 groups, one where damaged liver was caused by 25% ethanol and normal group administered with purified water. Results of test showed the expression of $TGF-{\beta}_1$, c-Myc, and $Thymosin-{\beta}_4$ genes was higher in the experimental group treated with 25% ethanol than in the normal group. Erb-B2 gene was not expressed clearly. Thus, it is considered that we can expect to utilize $TGF-{\beta}_1$, c-Myc 및 $Thymosin-{\beta}_4$ as auxiliary data and find clinical meanings of diagnosis on hepatic diseases, In addition to serologic and histological examination by convergence examining the gene expression status by molecular diagnostic techniques in liver-related disease prevention and diagnosis through results of this study.
Keywords
Hematoxylin; Eosin; $TGF-{\beta}_1$; c-Myc; Erb-B2; $Thymosin-{\beta}_4$; convergence;
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1 D. Safer, M. Elzinga, V. T. Nachmias. (1991). Thymosin beta4 and Fx, an actin-sequestering peptide, are indistinguishable. J. Biol. Chem, 266, 4029-4032.
2 D. Safer, R. Golla, V. T. Nachmias. (1990). Isolation of a 5-kilodalton actin-sequestering peptide from human blood platelets. Proc. Natl. Acad. Sci. USA, 87, 2536-2540.   DOI
3 D. Pantaloni, M. F. Carlier. (1993). How profiling promotes actin filament assembly in the presence of thymosin b4. Cell, 75, 1007-1014.   DOI
4 F. Kang, D. L. Purich, F. S. Southwick. (1999). Profilin promotes barbed- end actin filament assembly without lowering the critical concentration. J Biol Chem, 274, 36963-36972.   DOI
5 D. S. Grant, J. L. Kinsella, M. C. Kibbey, S. LaFlamme, P. D. Burbelo, A. L. Goldstein, H. K. andKleinman. (1995). Matrigelinducesthymosin beta4 gene in differentiating endothelial cells. J Cell Sci, 108, 3685-3694.
6 D. S. Grant, W. Rose, C. Yaen, A. Goldstein, J. Martinez, H. Kleinman. (1999). Thymosin-${\beta}4$ enhances endothelial cell differentiation and angio genesis. Angio genesis, 3, 125-135.
7 T. Huff, C. S. Muller, A. M. Otto, R. Netzker, E. Hannappel. (2001). beta-Thymosins, small acidic peptides with multiple functions. Int J Biochem Cell Biol, 33, 205-209.   DOI
8 J. Gomez-Marquez, J. I. Pedrares, A. Otero, R. Anadon. (1993). Prominent expression of the actin-sequestering peptide Fx gene in the hippo campal region of ratbrain. Neuroscience Letters, 152, 41-44.   DOI
9 J. Gomez-Marquez, F. F. delAmo, P. Carpintero, R. Anadon. (1996). High levels of mouse thymosin b4 mRNA in differentiating P19 embryonic cells and during development of cardiovascular tissues. Biochim BiophysActa, 1306, 187-193.
10 R. Anadon, I. R. Moldes, P. Carpintero, G. Evangelatos, E. Livianou, L. Leondiadis, I. Quintela, M. C. Cervino, J. Gomez-Marquez. (2001). Differential expression of thymosin b4 and b10 during rat cerebellum postnatal development. Brain Res, 894, 255-265.   DOI
11 D. Philp, A. L. Goldstein, H. K. Kleinman. (2004). Thymosin ${\beta}4$ promotes angiogenesis, wound healing, and hair follicle development. Mech Ageing Dev, 125, 113-115.   DOI
12 W. S. Wang, P. M. Chen, H. L. Hsiao, S. Y. Ju, Y. Su. (2003). Over expression of the thymosin beta-4 gene is associated with malignant progression of SW480 colon cancer cells. Oncogene, 22, 3297-3306.   DOI
13 K. M. Malinda, A. L. Goldstein, H. K. Kleinmman. (1997). Thymosin beta 4 stimulates directional migration of human umbilical vein endothelial cells. FASEG J, 11, 474-481.   DOI
14 E. A. Clark, T. R. Golub, E. S. Lander, R. O. Hynes. (2000). Genomics analysis of metastasis reveals an essectial role for RhoC. Nature, 406, 532-535.   DOI
15 T. Kobayashi, F. Okada, N. Fujii, N. Tomita, S. Ito, H. Tazawa. (2002). Thymosin-beta 4 regulates motility and metastasis of malignant mouse fibrosarcoma cells. Am J Pathol, 160, 869-82.   DOI
16 K. H. Kim, K. J. Jo, Y. Go. (2011). Understanding and technique of genetics, Korea medical book, 203-205.
17 M. Matsuoka, H. Tsukamoto. (1990). stimulation of hepatic lipocytie collagen pruduction by Kupffer cell-derived transforming growth factor B: implication for a pathogentic role in liver fibrogenesis. Hepatology, 11, 599-605.   DOI
18 H. Nakatsukase, R. P. Evarts, C. C. Hsia, S, S. Thorgeirsson. (1990). Transfoming growth factor-B1 and type I procollagen transscripts during regeneration and early fibrosis of rat liver. Lab Invest, 63, 171-180.
19 Z. Qi, N. Atsuchi, A. Ooshima, A. Takeshita, H. Ueno. (1999). Blockade of type B transforming growth factor sigaling prevents liver fibrosis and dysfunction in the rat, Proc Natl Acad Sci, 96, 2345-2349.   DOI
20 A. Castilla, J. Prieto, N. Fausto. (1991). Transforming growth factor B1 and a in chronic liver disease. N Engl J Med, 324, 933-940.   DOI
21 Y. S. Choi, (2017), The Regulatory Role of Dickkopf-1 as a Target of Oncogenic Transforming Growth Factor-${\beta}1$-Smad3 Pathway in Human Lung Cancer Cells, gangwon univrsity Graduate school, 1-2.
22 J. H. Park, (2016), The association of single nucleotide polymorphisms in the TGF-${\beta}1$ and RAGE genes with polycystic ovary syndrome, Cha university General Graduate Shool, 2-3.
23 J. H. Lee , C. G, Yoon, S. I. Lee. (1993). Effects of dietary protein on the changes of lipoprotein fractions in carbon tetrachloride-treated rats. J Korean Soc Food Sci Nutr, 22, 127-131.
24 J. L. Kenneth, D. S. Thomas. (2001). Analysis of relative gene expression data using real-time Quantitative PCR and the 2-DDCT method. Methods, 25, 402-408.   DOI
25 Y. H. You, K. Y. Jung, Y. H. Lee, W. J. Jun, B. Y. Lee. (2009). Hepatoprotective effects of hovenia dulcis fruit on ethanol-induced liver damage in vitro and in vivo. J Korean Soc Food Sci Nutr, 38, 154-159.   DOI
26 H. Rouach, M. Clement, M. T. Ofanelli, B. Janvier, J. Nordmann, R. Nordmann. (1983). Hepatic lipid peroxidation and mitochondrial susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochem. Biophys, Acta, 753, 439-444.   DOI
27 D. A. Brenner. (1991). Transforming growth factor B and hepatic fibrosis: cause of effect?, Hepatology, 14, 740-742.
28 Y. T. Ahn, J. S. Bae, Y. H. Kim, K. S. Lim, C. S. Huh. (2005). Effects of fermented milk intake on hepatic antioxidative systems in alcohol treated rats. Korean J Food Sci Technol, 37, 631-635.
29 P. Sher, (1997). Diagnostic effectiveness of biochemical liver-function tests, as evaluated by discriminant function analysis. Clin Chem, 23, 627- 630.
30 V. A. Ramchandani, W. F. Bosron, T. K. Li. (2001). Research advances in ethanol metabolism. Pathol. Biol, 49, 676-682.   DOI
31 C. S. Lieber. (1994). Alcohol and liver :update. Gastroenterology, 106, 1085-1090.   DOI
32 S. Zakhari. (2006). Overview: how is alcohol metabolized by the body? Alcohol Res & Health, 29, 245-254.
33 C. S. Lieber. (1970). New pathway of ethanol metabolism in the liver. Gastroenterology, 59, 930-937.
34 H. L. Bleich, E. S. Boro. (1977). Metabolic and hepatic effects of alcohol. N Engl J Med, 296, 612-616.   DOI
35 C. S. Lieber. (2004). Milestones in liver disease. J Hepatol, 40, 198-202.   DOI
36 A. Gramenzi, F. Caputo, M. Biselli, F. Kuria, E. Loggi, P. Andreone, M. Bernardi. (2006). Review article: alcoholic liver disease-pathophysiological aspects and risk factors. Aliment Pharmacol Ther, 24, 1151-1161.   DOI
37 S. K. Das, D. M. Vasudevan. (2007). Alcohol-induced oxidative stress. Life Sci, 81, 177-187.   DOI
38 K. Nagata, H. Suzuki, S. Sakaguchi. (2007). Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis. J Toxicol Sci, 32, 453-468.   DOI
39 T. Byers, G. Perry. (1992). Dietary carotenes, vitamin C, and vitamin E as protective antioxidants in human cancers. Ann Rev Nutr, 12, 135-159.
40 R. A. Lawrence, R. F. Burk. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun, 71, 952- 958.   DOI
41 T. L. Low, A. L. Goldstein. (1982). Chemical characterization of thymosin beta-4. J. Biol. Chem, 257, 1000-1006.
42 M. J. Kim, (2017). A study on Expression of Thymosin ${\beta}_4$ in Cervical Cancer induced Human Papilloma Virus, konyang university Graduate school. 7-8.
43 K. C. Hong, (2015). Overexpression and characterization of thymosin beta-4 in Escherichia coli, Dongwee univrsity Graduate school, 10-11.
44 K. O. Hong, (2016). Thymosin b4 induces proliferation, invasion, and epithelial-to-mesenchymal transition of oral squamous cell carcinoma, seoul univrsity Graduate school, 1-2.
45 S. H. Yoon. (2008.). Expression of Thymosin-${\beta}4$ in odontoblast differentiation of the mouse. Josun Univ. Graduate school.