• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.8
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• pp.1187-1193
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• 2010

To elongate the shelf-life of Angelica keiskei and Brassica loeracea var. acephala vegetable juices, UV irradiation was used and the changes of antioxidant activity and nitrite scavenging ability were investigated. The content of polyphenols of vegetable juices were slightly reduced by UV treatment and/or storage period. The DPPH radical-scavenging activities of the vegetable juices treated by UV were higher than that of control but were not changed during storage. However, $ABTS{\cdot}^+$ reducing activities of the vegetable juices were reduced by UV treatment. The $ABTS{\cdot}^+$ reducing activity of Brassica loeracea var. acephala juice was lower when the flow rate was slower. The ferrous ion chelating effects of Angelica keiskei vegetable juices were reduced by UV treatment. In contrast, the ferrous ion chelating effects of Brassica loeracea var. acephala vegetable juices were not different from those of right after manufacturing. The ferrous ion chelating effects on both vegetable juices increased during storage periods. The inhibitory activity of lipid oxidation was decreased slightly by UV treatment on vegetable juices. The nitrite scavenging ability of Angelica keiskei and Brassica loeracea var. acephala vegetable juices treated by UV irradiation was not different from that of control. The nitrate scavenging abilities of vegetable juices in pH 1.2 were higher than those in pH 3.0 and 4.2.

• Herbal Formula Science
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• v.21 no.1
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• pp.177-185
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• 2013

Objectives : Leejung-tang (Lizhong-tang) has been used for treatment of gastrointestinal disorders in Korea. In this study, we performed quantification analysis of five marker components, liquiritin, ginsenoside Rb1, ginsenoside Rg1, glycyrrhizin, and 6-gingerol in Leejung-tang using a ultra performance liquid chromatography- electrospray ionization-mass spectrometer (UPLC-ESI-MS). In addition, we evaluated antioxidant activity of Leejung- tang. Methods : The column for separation of five constituents used a UPLC BEH C18 ($100{\times}2.1mm$, $1.7{\mu}m$) maintained at $45^{\circ}C$. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in H2O (A) and CH3CN (B) by gradient flow. The flow rate was 0.3 mL/min with detection at mass spectrometer. The antioxidative activities conduct an experiment on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of Leejung-tang. Results : Calibration curves of five marker compounds were acquired with r2 values > 0.99. The amount of the five compounds in Leejung-tang were 0.07 - 0.84 mg/g. The concentration required for 50% reduction (RC50) against ABTS radical was 119.02 ug/mL. In addition, the scavenging against DPPH radical of Leejung-tang was 11.4%, 14.5%, 19.8%, 29.6%, and 49.2% at 25 ug/mL, $50{\mu}g/mL$, $100{\mu}g/mL$, $200{\mu}g/mL$, and $400{\mu}g/mL$, respectively. Conclusions : The established LC-MS/MS method will be helpful to improve quality control of Leejung-tang. In addition, Leejung-tang is a potential antioxidant therapeutic agent.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.489-493
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• 2003

This study was to search antioxidant constituents of ethyl acetate extract from Stachys siebodlii MIQ. The test of nitrite scavenging abilities were performed on the extracts of methanol, hexane, chloroform, ethyl acetate, butanol, and water, Ethyl acetate extract, The most promising one was fractionated on a silical gel column using elution solvent(chloroform:methanol:water=70:30:5 lower phase) at a flow rate 1.0ml/min. UV-VIS spectral data of each fraction showed adsorption maxima in the range of $284{\sim}330nm$ which is the characteristic range of $210{\sim}290nm$ and $300{\sim}550nm$ phenolic compounds. These results suggested that Stachys siebodlii MIQ. shows natural antioxidant activity. The nitrite scavenging abilities reached the maxium at pH 1.2 and the ethyl acetate fraction of root showed stronger scavenging ability.

• Journal of Life Science
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• v.17 no.12
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• pp.1634-1640
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• 2007

The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), nitric oxide (NO) scavenging activities and ferric- reducing/antioxidant power (FRAP) assay against extracts of Kalopanax pictus (KP) were measured. Radical scavenging and antioxidant activities were increased depend on the concentration and the effects were enhanced by ascorbic acid (AA). KP extracts and AA had a good anti-proliferating activity against HepG2 cells by MTT assay and induces cells apoptosis, which was demonstrated by flow cytometric analysis. KP extracts and AA caused the arrest of cell-cycle progression at either G0-G1-phase or G2/M-phase, which might be depending upon the KP extracts concentration. In addition, KP extracts and AA are effective in enhancing immunity and nitric oxide production by RAW 264.7 macrophages cells. KP extracts and AA inhibited tumor cell growth and exerted antioxidant effects as compared to controls. These results demonstrate that simultaneous AA and KP extracts treatment could be useful in preventing the oxidative damage and anti-proliferating HepG2 cells, and are effective in enhancing immunomodulatory and antioxidant activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.2
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• pp.239-245
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• 2013

In in vitro study, the anti-platelet aggregation effect of Shiitake mushroom extract was examined by measuring the collagen induced platelet aggregation and the DPPH radical scavenging. In in vitro study, anti-thrombotic effect of Shiitake mushroom extract was examined using the carotid artery thrombosis rat model. Carotid artery thrombosis rat model was made by 35% $FeCl_3$ treatment. After that, we investigate thrombus weight and blood flow. In platelet aggregation test, the extract significantly inhibited platelet aggregation in a concentration dependent manner(p<.001). Also, extract increased DPPH radical scavenging activity in a concentration dependent manner. Extract significantly inhibited thrombus weight to compare with control group. And blood passage time were shorter in the Shiitake mushroom extract supplemented groups than in control group. These results provide experimental evidence that Shiitake mushroom can be used to prevent platelet aggregation and thrombosis, then could apply the clinical diseases such as cardiovascular disease, and so on.

• Transactions of the Korean Society of Automotive Engineers
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• v.4 no.1
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• pp.165-176
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• 1996

The flow field and spray characteristics for loop scavenged type 2stroke engine having pancake shape was numerically computed using KIVA-Ⅱ code. The cylinder has 1intake port, 2side intake ports and 1exhaust port with induced flow angle 25 deg. In engine calculation, the chop techniques is used to strip or add planes of cells across the mesh adjacent to the TDC and the BDC(ports parts) for preventing the demand of exceed time during the computation, providing a control on cell height in the squish region. The modified turbulent model including the consideration of the compressibility effect due to the compression and expansion of piston was also used. The case of 25 deg.(injection angle) which is opposite to scavenging flow direction shows better the distribution of droplets and the evaporation rate of droplets compared to other cases(0 deg., - 25 deg.). When injection pressure was increased, the spray tip penetration became longer. When injection pressure was increased, the interaction between the upward gas velocity and spray droplets strongly cause. Thus the breakup of droplets is strongly occurred and the evaporation rate of droplets was found to be better.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.2
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• pp.78-88
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• 2012

Objectives: Paeonia japonica has been widely used for gynecopathy and analgesic effects in Korean Traditional Medicine. The aim of the present study is to determine the antioxidant effect of Paeonia japonica extracts(PJE) by using mouse embryonic fibroblast cells(MEF cells). Methods: We evaluated Radical Scavenging Activity of PJE by the DPPH assay. Protective effect of the PJE on the hydrogen peroxide($H_2O_2$) induced oxidative damage of MEF cells was analyzed by the MTT assay. The Morphological changes of MEF cells induced by P. japonica, $H_2O_2$ and P. japonica+$H_2O_2$ was evaluated by DAPI staining. And effect of PJE on the rate of apoptosis in MEF cells was measured using flow cytometry with Annexin V-FITC and PI double staining. Results: We observed that PJE contain significant DPPH radical scavenging activity. Cell viability of oxidative damaged cells treated with various concentrations of $H_2O_2$ was increased by treatment with PJE. Flow cytometric analysis of the cells treated with $H_2O_2$ in the absence or presence of PJE showed that the crumbled G1 peak was accumulated by the treatment with $H_2O_2$ alone, but restored by addition of PJE. Portion of cells that undergo apoptosis mediated by oxidative stress was decreased by treatment of PJE. The nuclear fragmentation occurred in the oxidative damaged MEF cells was also decreased by PJE treatment. Conclusions: Taken together, our results suggest that PJE exhibits significant antioxidant activity and functions to inhibit cell death mediated by oxidative damage induced apoptotic pathways.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.5
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• pp.411-415
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• 2001

Fifty-four Korean native and 28 foreign rice varieties harvested in 1998 and 1999 were examined for antioxidative activity that is measured to a chemiluminescence and superoxide radical intensity, by the flow injection chemiluminescence (FI-CL) system and an electron spin resonance (ESR) spectrophotometer, respectively. In the chemiluminescence measurement by FI-CL, radical scavenger activity did not differ significantly among rice varieties between origin types of rice varieties, and between storage periods. GinSun and Hongchoengdo, colored rice exhibited high electron scavenging effect by ESR. Therefore, these results indicate that the pigments of rice varieties may play important antioxidative roles and that it may be possible to breed rice varieties with higher antioxidative potentials.

• Journal of Hydrogen and New Energy
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• v.22 no.6
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• pp.794-799
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• 2011

In order to reduce the oil consumption for a 2 stroke free piston hydrogen fueled engine, the behaviors of residual lubricant oil of the cylinder wall surface were visualized and oil mass emitted into exhaust port was measured by using research engine with cross-head and eccentric crankshaft. As the results, it was shown that characteristics of residual lubricant oil such as oil thickness and distribution were remarkably different from a conventional 4 stroke engine. It was also analyzed that these tendencies relied on the configuration and installed position of the exhaust port, piston pin boss and so on.

• Journal of Society of Preventive Korean Medicine
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• v.17 no.3
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• pp.165-176
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• 2013

Objective : The purpose of this study was to establish the simultaneous analysis for six compounds in Gumiganghwal-tang (GMGHT, Jiuweiqianghuo-tang) and to investigate the anti-atherosclerotic effects of GMGHT in vitro. Methods : The column for separation of six compounds was used Luna $C_{18}$ column and maintained at $40^{\circ}C$. The mobile phase for gradient elution consisted of two solvent systems, 1.0% acetic acid in water and 1.0% acetic acid in acetonitrile. The analysis was carried out at a flow rate of 1.0 mL/min with pothodiode array (PDA) detection at 254, 280, and 320 nm. The injection volume was 10 ${\mu}L$. The antioxidant activities of GMGHT were evaluated by measuring free radical scavenging activities on 2,2'-Azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The inhibitory effects on low-density lipoprotein (LDL) oxidation were evaluated by the formation of thiobarbituric acid relative substances (TBARS), relative electrophoretic mobility (REM), and fragmentation of apolipoprotein B (ApoB)-100. Results : Calibration curves were acquired with $r^2{\geq}0.9998$. The contents of liquiritin, ferulic acid, baicalin, baicalein, glycyrrhizin, and wogonin in GMGHT were 1.784, 1.693, 37.899, 0.258, 1.869, and 0.034 mg/g, respectively. The GMGHT showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction ($RC_{50}$) against ABTS and DPPH radicals were 72.51 ${\mu}g/mL$ and 128.49 ${\mu}g/mL$. Furthermore, GMGHT reduced the oxidation properties of LDL induced by $CuSO_4$. Conclusion : HPLC-PDA is considered as an available and convenient method for quality control and standardization of GMGH and GMGHT has potentials on anti-atherosclerosis by anti-oxidative effect and suppressive effect on LDL oxidation.