• Title/Summary/Keyword: Scavenging

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Enhanced DPPH Radical Scavenging Activity of Lactobacillus plantarum K-21 Isolated from Kimchi and its Various Antioxidant Effects (김치유래 Lactobacillus plantarum K-21의 DPPH 라디칼 제거활성 증진 및 다양한 항산화 효과)

  • Kim, Yerin;Kim, Yedam;Jeon, Chae-Min;Park, Gyulim;Lee, O-Mi;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.31 no.8
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    • pp.715-725
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    • 2022
  • Lactic Acid Bacteria (LAB) are among the representative probiotics that have been used for a long time in fermented food. Although there are many studies on detecting the radical scavenging activity of LAB, few studies have been conducted on the environmental factors that improve scavenging activity. This study investigated the environmental factors affecting the DPPH radical scavenging and various antioxidant activities of Kimchi-derived Lactobacillus plantarum K-21 with antihypertensive and radical scavenging activities. The optimal conditions for scavenging DPPH radicals were glucose 2%, bactopeptone 0.5%, Tween 80 0.05%, L-cysteine 0.05%, and an initial pH 6.5 at 35℃. Under optimal conditions, the DPPH radical scavenging activity was 94.8±2.2%, which was 1.5 times higher than that of the basic medium. In addition, L. plantarum K-21 had other antioxidant activities; ABTS radical scavenging (93.6±1.5%), hydroxyl radical scavenging (8.5±0.9%), metal chelating (65.9±0.5%), NO scavenging (53.1±19%), SOD-like (25.1±1.5%), and reducing power (11.7±1.4%) activities were detected. Therefore, L. plantarum K-21 may act not only as a starter for lactic acid-fermented foods with improved functionality but also as a drug for various diseases caused by oxidative stress.

A Flow Analysis for Improvement of the Loop Scavenging Performance of a Free Piston Linear Power System (프리피스톤 리니어 동력시스템의 루프소기성능 향상을 위한 유동해석)

  • Yoon, Jae-Seong;Cho, Hyoung-Wook;Lee, Jong-Tai;Lee, Yong-Gyun
    • Transactions of the Korean hydrogen and new energy society
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    • v.19 no.2
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    • pp.139-144
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    • 2008
  • The focus of this research is that the scavenging aspect of in-cylinder is visualized by the PIV method and its characteristic is analyzed so that the scavenging performance of the free piston hydrogen fueled engine can improve with loop scavenging. As the results, the piston of convex type shows the best scavenging performance among the presented pistons. In case of the abnormal expansion, the scavenging of area between cylinder head and cylinder wall doesn't operate well.

Radical Scavenging Effect of Methanol Extracts from Seaweeds and Their Active Compounds (해조류 추출물 및 활성성분의 라디칼 소거능)

  • So, Mi Jung;Cho, Eun Ju
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.3
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    • pp.187-191
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    • 2007
  • The radical scavenging activity of methanol extracts of seaweeds and their active compounds, alginic acid, fucoidan and phloroglucinol, were investigated under in vitro. Among methanol extracts of seaweeds (sea mustard, sea tangle, seaweed papulosa, fusiforme, sea lettuce, purple laver and chlorella), seaweed papulosa and sea tangle showed strong scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical (${\cdot}OH$). In addition, under in vitro, the scavenging activities on DPPH radical of alginic acid and fucoidan, which are active compounds of brown algae, and phloroglucinol, the active compound from Ecklonia species, were evaluated and compared. Fucoidan and phloroglucinol showed strong DPPH scavenging effect, in particular, phloroglucinol had strongest activity among the active compounds. On the other hand, alginic acid did not exert DPPH scavenging activity. From the present study, we could confirm the antioxidative activity of seaweeds and its active compounds.

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Effect of Heating on DPPH Radical Scavenging Activity of Meat Substitute

  • Song, Hyeun Sung;Bae, Jun Kyu;Park, Inshik
    • Preventive Nutrition and Food Science
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    • v.18 no.1
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    • pp.80-84
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    • 2013
  • This study was conducted to evaluate the increase of DPPH radical scavenging activity of meat substitute by heating. The meat substitute showed higher DPPH radical scavenging activity than those of other foods rich in protein such as beef, pork, chicken, and soybean curd. The DPPH radical scavenging activity of meat substitute was dependent upon concentration, heating temperature and heating time of meat substitute. The DPPH radical scavenging activity of meat substitute was enhanced with increasing heating temperature and time. The increase of DPPH radical scavenging activity was only applied to meat substitute without showing any activation in other foods rich in protein such as beef, pork, chicken, and soybean curd.

Flow visualization used PIV of hydrogen fueled free piston engine with uni-flow scavenging (PIV를 이용한 Uni-flow 소기방식 프리피스톤 수소기관의 실린더내 유동가시화)

  • Cho, H.W.;Yoon, J.S.;Lee, J.T.;Lim, H.S.
    • Transactions of the Korean hydrogen and new energy society
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    • v.19 no.3
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    • pp.182-188
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    • 2008
  • In order to improve scavenging performance of free piston hydrogen fueled engine, this study estimate compatibility of uni-flow scavenging. The scavenging flow characteristics in the cylinder is investigated by flow visualization and PIV method. Consequently it has been found that the scavenging performance decreased with abnormal expansion of piston and delay of the exhaust valve opening timing. And the scavenging performance of exhaust valve located center in cylinder head is better than that of exhaust valve located side in cylinder head.

An Improved method in Screening of Superoxide and Hydroxyl Radical Scavenging Activities of Plant Medicinal Extracts (생약 추출물에 의한 superoxide와 hydroxyl 라디칼 소거능 검색 방법의 개선)

  • Lee, Ho-Sub;Kang, Dae-Gill
    • Korean Journal of Pharmacognosy
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    • v.32 no.3 s.126
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    • pp.253-256
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    • 2001
  • The present study was designed for the improvement of routine measurement of superoxide and hydroxyl radical scavenging activities utilized by a microplate reader. Superoxide radical scavenging activity by the ascorbic acid, which is a well-known superoxide scavenger, was determined in a dose-dependent manner. Hydroxyl radical scavenging activity by the thiourea, which is a well-known hydroxyl radical scavenger, was also well detected in a dose-dependent manner. Our results suggest that the use of microplate reader to assay the superoxide and hydroxyl radical scavenging activities improves the accuracy of data and enables the use of much smaller amounts of samples and/or reagents, with much simpler experimental procedure. Therefore, These methods appear to be suitable for screening of superoxide and hydroxyl radical scavenging activities in both the plant medicinal extracts and the isolated compounds.

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A Study on the Scavenging Efficiency Evaluation for the RSSV Configuration of 2-Stroke Engine with Popet Valve Type Using Single-Cycle Method (Single-Cycle 기법을 이용한 포핏밸브형 2-행정기관의 RSSV 형상에 따른 소기효율 측정에 관한 연구)

  • 이진욱;강건용;정용일;이주헌;박정규
    • Transactions of the Korean Society of Automotive Engineers
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    • v.5 no.2
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    • pp.69-79
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    • 1997
  • This paper deals with the measurement and analysis on the scavenging performance of the oppet-valve type two-stroke engine with different shroud system. The scavenging flow characteristics is investigated by flow visualization under steady condition, in which a dye is introduced into single-cycle method using the difference of specific gravity between two working fluids is used to evaluate the scavenging efficiency and the trapping efficiency. The 90° shroud system was found to be the highest efficiency system through both flow visualization and single-cycle test, as well as the shroud system to generally be efficient for reducing a short-circuiting flow during scavenging process in a two-stoke engine.

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Antioxidant activity of ethanol extracts from the root and bark of Ulmus davidiana

  • Sim, Ki Hyeon;Han, Young Sil
    • Korean Journal of Food Science and Technology
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    • v.51 no.2
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    • pp.133-140
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    • 2019
  • Antioxidant activities of Ulmus davidiana root and bark extracts were evaluated by various antioxidant tests, including DPPH radical-scavenging, nitric oxide-scavenging, superoxide anion radical-scavenging, and ABTS radical-scavenging assays, and superoxide dismutase (SOD)-like activity and reducing power analysis, along with the determination of total phenolic and flavonoid contents. Both the extracts showed strong antioxidant activities by these testing methods. Ulmus davidiana root extract possessed strong reducing power and nitric oxide-scavenging activity, and high scavenging activities against free radicals including the superoxide anion, and the ABTS and DPPH radicals, but a weaker scavenging activity of SOD. In contrast, the Ulmus davidiana bark extract exhibited a strong SOD-like activity, but all the other activities were weak. It was observed that the total phenolic and flavonoid contents of the Ulmus davidiana root extract were higher than those of the Ulmus davidiana bark extract.

Scavenging Property of Pyungwi-san Herbal-acupuncture Solution on ROS and RNS (평위산(平胃散) 약침액(藥鍼液)의 활성산소 및 활성질소 소거능)

  • Lee, Hyo-Seung;Moon, Jin-Young
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.1
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    • pp.165-170
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    • 2007
  • Pyungwi-san(PWS) have been using as a basic prescription of digestive disorder in Korean traditional medicine. This study was performed to examine the in vitro antioxidant activity of the extract using different antioxidant tests including by 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, superoxide anion radical scavenging, metal chelating hydrogen peroxide scavenging, lipid peroxydation protective effect and scavenging effect of nitric oxide and peroxynitrite. Herbal-acupuncture solution of PWS(PWS-HS) exhibited a concentration-dependent inhibition of DPPH radical adduct formation and it showed dose-dependent free radical scavenging activity onto superoxide anions. In addition, the result of metal chelating hydrogen peroxide scavenging and ammonium thiocyanate experiments showed that PWS-HS was an active scavenger of hydroxyl radicals. Furthermore, it was also found to be effective in scavenging nitric oxide and peroxynitrite, well-known cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA.

Radical Scavenging Activity of Ethanol Extracts and Solvent Partitioned Fractions from Various Red Seaweeds (홍조류 에탄올 추출물 및 다양한 용매 분획물의 라디칼 소거능)

  • Cho, MyoungLae;Lee, Dong-Jin;You, SangGuan
    • Ocean and Polar Research
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    • v.34 no.4
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    • pp.445-451
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    • 2012
  • The EtOH extracts of red seaweeds (Symphyocladia latiuscula, Chondrus ocellatus and Carpopeltis affinis) and solvent partitioned fractions were investigated for their 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effects and the total phenolic contents were correlated with ABTS and DPPH radical scavenging activities. The EtOH extracts and their solvent partitioned fractions exhibited strong ABTS and DPPH radical scavenging activities. Among the solvent partitioned fractions obtained from n-Hexane (HX), methylenchloride (MC), ethylacetate (EA), and buthanol (BuOH), the HX fraction from C. affinis showed higher radical scavenging activities than other fractions. Total phenolic contents showed significant correlation ($r^2$ = 0.709) with ABTS radical scavenging activity. The results of this study suggest that the strong radical scavenging activity of HX fraction from C. affinis is a promising natural antioxidant for healthcare products.