Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Scavenging Property of Pyungwi-san Herbal-acupuncture Solution on ROS and RNS

평위산(平胃散) 약침액(藥鍼液)의 활성산소 및 활성질소 소거능

  • Lee, Hyo-Seung (Cardiovascular Medical Research Center and Department of AM-Pointology, College of Korean Medicine, Dongguk University) ;
  • Moon, Jin-Young (Cardiovascular Medical Research Center and Department of AM-Pointology, College of Korean Medicine, Dongguk University)
  • 이효승 (심혈관계질환 천연물개발연구센터 & 동국대학교 한의과대학 경혈학교실) ;
  • 문진영 (심혈관계질환 천연물개발연구센터 & 동국대학교 한의과대학 경혈학교실)
  • Published : 2007.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Pyungwi-san(PWS) have been using as a basic prescription of digestive disorder in Korean traditional medicine. This study was performed to examine the in vitro antioxidant activity of the extract using different antioxidant tests including by 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, superoxide anion radical scavenging, metal chelating hydrogen peroxide scavenging, lipid peroxydation protective effect and scavenging effect of nitric oxide and peroxynitrite. Herbal-acupuncture solution of PWS(PWS-HS) exhibited a concentration-dependent inhibition of DPPH radical adduct formation and it showed dose-dependent free radical scavenging activity onto superoxide anions. In addition, the result of metal chelating hydrogen peroxide scavenging and ammonium thiocyanate experiments showed that PWS-HS was an active scavenger of hydroxyl radicals. Furthermore, it was also found to be effective in scavenging nitric oxide and peroxynitrite, well-known cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA.

Keywords

References

  1. Hur, E.S. and Lee K.H. Cardiovascular Disease and Natural Antioxidants. Journal of Human Ecology 5:19-35, 2001
  2. Diane L.T. Antioxidant Consuption and Risk of Coronary Heart Disease: Emphasis on Vitamin C, Vitamin E, and ${\beta}$-carotene. Circulation 99:591-595, 1999 https://doi.org/10.1161/01.CIR.99.4.591
  3. 조성희. Protagonist of Antioxidant. 한국지질학회.대한동맥경화학회 추계공동학술대회집, 81-85, 2000
  4. Hou, Y.Z., Zhao, G.R., Yang, J., Yuan, Y.J., Zhu, G.G., Raimo, H. Protective dffect of Lingusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide. Life Science 75:1775-1786, 2004 https://doi.org/10.1016/j.lfs.2004.05.005
  5. 金相贊.金先熙.盧昇鉉 外 8人. 方劑學. 영림사, 서울, pp 486-487, 1999
  6. 허 준. 동의보감. 법인문화사, 서울, pp 661-668, 1999
  7. Park, D,W., Kim, J,S., Ryu, B,H., Ryu, K,W., Park, S,H. A Study on the Effects of Pyungwee-san and Compounding of Medicine in Pyungwee-san on the Movement of Gastrointestinal Smooth Muscles, Anticatharsis and Central Nervous System. J. of. Oriental. Chr. Dis. 6:184-196, 2000
  8. Jeong, H.C. and Son, I,C. Effects of Pyeongwoe-San Aqua - Acupuncture at Chok-Samni ( ST 36 ) points on Gastric in Rabbits. The J. of Korean Acupuncture ＆ MoxibustioMotility n Society 13:321-334, 1996
  9. 黃度淵 原著.裵元植 監修, 對譯證脈方藥合編, 南山堂, 서울, 142-143, 2000
  10. 錢百炎, 中草藥主射劑, 上海科學技術出版社, 上海, 71-132, 1981
  11. Gyamfi, M.A., Yonamine, M. and Aniya, Y. Free-radical scavenging action of medicinal herbs from Ghana. Gen. Pharmacol 32:661-667, 1999 https://doi.org/10.1016/S0306-3623(98)00238-9
  12. Gotoh, N. and Niki, E. Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence. Biochim. Biophys. Acta. 1115:201-207, 1992 https://doi.org/10.1016/0304-4165(92)90054-X
  13. Halliwell, B., Gutteridge. J.M.C. and Aruoma, O.I. The deoxyribose method: simple "test-tube" assay for determination of rate constants for reaction of hydroxyl radicals. Anal. Biochem. 165:215-219, 1987 https://doi.org/10.1016/0003-2697(87)90222-3
  14. Osawa, T., and Namiki, M. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agric. Biolog. Chem. 45:735-740, 1981 https://doi.org/10.1271/bbb1961.45.735
  15. Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1:145-157, 1997 https://doi.org/10.1006/niox.1996.0113
  16. Sutherland, H., Khundkar, R., Zolle, O., McArdle, A., Simpson, A.W., Jarvis, J.C. and Salmons, S. A fluorescence-based method for measuring nitric oxide in extracts of skeletal muscle. Nitric Oxide 5:475-481, 2001 https://doi.org/10.1006/niox.2001.0374
  17. Delanty, N. and Dichter, M.A. Oxidative injury in the nervous system. Acta Neurol Scand 98:145-153, 1998 https://doi.org/10.1111/j.1600-0404.1998.tb07285.x
  18. Brune, B., Zhou, J. and Von Knethen, A. Nitric oxide, oxidative stress and apoptosis. Kidney Int Suppl. 84:22-24, 2003
  19. Philip, M. The use of the stable free radical diphenylricrylhydrazyl (DPPH) for estimating antioxidant. Songklanakarin J. Sci. Technol. 26:211-219, 2004
  20. Abdalla, A.E. and Roozen, J.P. Effect of plant extracts on the oxidative stability of sunflower oil and emulsion. Food Chem. 64:323-329, 1999 https://doi.org/10.1016/S0308-8146(98)00112-5
  21. 大柳善疹, SODよ活性酸素調節濟よの 藥理作用よ 臨床應用, 日本醫學館, 東京, p 9, 1989.
  22. Ameczua, J.L., Palmer, R.M.J., De Souza, B.M. and Moncada, S. Nitric oxide synthesized from L-arginine regulates vascular tone in the coronary circulation of the rabbit. Br J Pharmacol 97:1119-1124, 1989 https://doi.org/10.1111/j.1476-5381.1989.tb12569.x
  23. Francesco, V. and Roberto, C. Antioxidants and cardiovascular disease. Current Opinion in Investigation Drugs 6:895-900, 2005