• YAKHAK HOEJI
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• v.36 no.5
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• pp.474-480
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• 1992

Astilbes rhizoma has been used for headache, arthralgia, chronic bronchitis and stomachalgia in traditional chinese medicine. The analgesic activities and their components of Astilbe chinensis var. davidii Rhizomes were evaluated. The ether and ethylacetate fractions of 70% EtOH extract showed considerable analgesic activities by acetic acid induced writhing method. Compound $1{\sim}5$ were isolated from ethylacetate fraction and identified as gallic acid, (+)-catechin, (+)-gallocatechin, bergenin and 11-O-galloylbergenin on the basis of spectroscopic methods. Among them (+)-gallocatechin showed stronger analgesic activity than that of other compounds.

• Journal of Plant Biology
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• v.28 no.2
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• pp.95-104
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• 1985

Additional serological data from radial immunodiffusion and conventional immunoelectrophoresis with combination of presaturation of antibody were obtained for further interpretation of Cornus (and relatives) systematics. Pollen proteins were compared by qualitative and quantitative means. Cornus drummondi and C. stolonifera are very similar to C. florida, and C. amomum and C. recemosa are less similar. Nyssa constitutes the next distinct and most similar family, Nyssaceae to the Cornaceae. The serological affinities indicate that Corokia and Griselinia should not be included within the Cornaceae. Their taxonomic treatment to elevate to the family level is now awaiting until more data are accumulated.

• Archives of Pharmacal Research
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• v.27 no.10
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• pp.1060-1064
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• 2004

Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 3,23-dihydroxy- 20(29)-lupen-27-oic acid on the regulation of MMP-1 and type 1 procollagen in Ultraviolet irradiation of cultured old age human dermal fibroblasts. 3, 23-dihydroxy-20(29)-lupen-27- oic acid was isolated from Tiarella polyphylla D. Don (Saxifragaceae). Among them, 3, 23- dihydroxy-20(29)-lupen-27-oic acid induced the regulation of Type 1- procollagen and reduced the regulation of MMP-1 at the protein levels in a dose-dependent manner by ultraviolet irradiation. Taken together, our results suggest that 3, 23-dihydroxy-20(29)-lupen-27-oic acid plays an important role in the induction of Type 1-procollagen and reduction of MMP-1 by ultraviolet irradiation in old age human dermal fibroblasts.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.117-117
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• 2019

Kirengeshoma koreana Nakai (K. koreana)was Saxifragaceae and rare plants in Korea, which is classified as an Critically Endangered (CR) species in Korea. Therefore, most of the studies on it were ecological and taxonomic, and there are no studies on biological activity. In this study, we evaluated the whitening activity of K. koreana extract (KKE). Melanogenesis Inhibitory effects were demonstrated by western-bot and RT-PCR for the effects of KKE on MITF, tyrosinase, TRP-1 and TRP-2 in IBMX-treated B16F10 melanoma cells. IBMX were reported as melanin synthesis enhancers. It could increase intracellular melanin synthesis by activation of the microphthalmia-associated transcription factor (MITF) signaling pathway. KKE showed no cytotoxicity at B16F10. In addition, KKE effectively inhibited the protein and mRNA levels of MITF, tyrosinase, TRP-1 and TRP-2. In conclusion, KKE inhibited melanin synthesis by inhibiting the expression of MITF and its downstream pathways tyrosinase, TRP-1 and TRP-2. Therefore, it was confirmed that K. koreana is a valuable resource for functional cosmetic and biomaterials.

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.1-6
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• 2007

The vegetation of habitats of a Korean rare plant Kirengeshoma koreana Nakai at Mt. Baekun was investigated by phytosociological approach. The vegetation of investigated area shows typical southern-temperate Quercus forest. The humidity of soil was high and the ground of locality was flat area of sub-alpine. These investigated area is very important for conservation of a rare plant, Kirengeshoma koreana that is limited in this area. Therefore, sustainable conservation strategy such as designation of conservation area should be prepared urgently by the results of long-term monitoring studies.

• Natural Product Sciences
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• v.20 no.2
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• pp.113-118
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• 2014

Ribes fasciculatum which belongs to Saxifragaceae has been widely used as a traditional medicine for the treatment of symptoms associated with lacquer poison. However, pharmacological studies on the R. fasciculatum are extremely limited until now. Thus, in this study, we evaluated the possible anti-inflammatory effects of ethyl acetate fraction of R. fasciculatum (ERF) using IFN-${\gamma}$/LPS-stimulated peritoneal macrophage model. We investigated the change in nitrite level in the absence or presence of ERF after LPS stimulation, and we found that ERF effectively attenuates the NO production in a dose dependent manner without notable toxicity. To determine the mechanism of the inhibitory action of ERF on NO production, we performed iNOS enzyme activity assay and Western blotting. Here we showed that both of iNOS enzyme activities and iNOS expressions were significantly down-regulated by ERF, indicating that these dual activities of ERF are responsible for ERF-mediated NO suppression. In addition, ERF inhibitied the expression of cyclooxygenase-2 (COX-2), an another key enzyme in inflammation through suppression of NF-${\kappa}B$ activation. We also tested anti-inflammatory properties of ERF not only in vitro, but in vivo using trypsin-induced paw edema model in mice. Our results revealed that the increased paw volume in response to trypsin injection was recovered by ERF supplement dose dependently.

• Natural Product Sciences
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• v.22 no.3
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• pp.154-161
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• 2016

Phytochemical investigation on the leaves of Pileostegia viburnoides Hook.f.et Thoms led to the isolation of twenty-five compounds, and their structures were identified as n-dotriacontane (1), taraxeryl acetate (2), friedelin (3), epifriedelinol (4), canophyllal (5), stigmast-4-en-3-one (6), stigmasterol (7), (24R)-5A-stigmastane-3,6-dione (8), ursolic acid (9), pomolic acid (10), umbelliferone (11), 4-epifriedelin (12), n-octatriacontanol (13), ${\beta}$-amyrin (14), ${\alpha}$-amyrin (15), taraxerol (16), nonadecanol (17), friedelane (18), arachic acid (19), protocatechuic acid (20), n-pentatriacontanol (21), hexadecanoic acid (22), vincosamide (23), daucosterol (24), and skimming (25), respectively. To our best knowledge, compounds 1, 2, 12, 13, 17 - 19 and 21-23 were new within Saxifragaceae family. Compounds 15, 16, and 20 were produced from this genus for the first time. Compounds 4, 14 and 25 were first obtained from species P. viburnoides and compounds 3, 5 - 11, and 24 were achieved from the leaves of P. viburnoides for the first time. Furthermore, the anti-neuroinflammatory activity of these isolates was evaluated.

• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.1-9
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• 2004

Prolyl endopeptidase (PEP) is proline-specific serine protease, cleaving peptide bonds on the biologically active neuropeptides such as substance P, vassopressin, and thyrotropin-releasing hormone and is, therefore, suggested to play important roles in learning and memory process. In this work, the inhibitory effect of plant extracts on PEP was investigated. Out of 200 plant extracts, Prunus mume, Pyrola. japonica, Hypericum ascyron, Astilbe chinensis var. typica, and Elaeagnus umbellata inhibited more than 90% of PEP activity at the concentration of 5 ppm.

• Archives of Pharmacal Research
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• v.22 no.4
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• pp.428-431
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• 1999

Seven known oleanolic acid glycosides (1-7) were isolated form the MeOH extract of Tiarella polyphylla. The structures were identified to be 3-O-($\beta$-glucopyranosyl) oleanolic acid (1), 3-O-[$\beta$-D-glucopyranosyl-(1 3)-$\beta$-D-glucopyranosyl] oleanolic acid (2), 3-O-D-[$\beta$-D-glucopyranosyl-(1 2)-$\beta$-D-glycopyranosyl] oleanolic acid (3), 3-O-[$\beta$-D-glucopyranosyl-(1 3)-$\beta$-D-glucopyranosyl] oleanolic acid 28-O-$\beta$D-glucopyranosyl ester (4), 3-O-[$\beta$-D-glucopyranosyl-(1 2)-$\beta$-D-glucopyranosyl] oleanolic acid 28-O-$\beta$-D-glucopyranosyl ester (5), 3-O-[a-L-rahmnopyranosyl-(1 3)-$\beta$-D-glucururonopyranosyl] oleanolic acid (6), and 3-O-[$\alpha$-L-rhamnopyranosyl-(1 3)-$\alpha$-D-glucuronopyranosyl] oleanolic acid 28-O-$\alpha$-D-glucopyranosyl ester (7) on the basis of physicochemical and spectral data. These triterpene glycosides were tested for the anti-complement activity and hemolytic activity. Bisdesmosidic saponins, 4, 5, and 7, showed anti-complement activity; in contrast, monodesmosidic saponins, 1-3, and 6, showed direct hemolytic activity. Methyl esterified monodesmosidic saponins showed anti-complement activity at a low concentration and hemolytic activity at a high concentration.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.1
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• pp.35-51
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• 2012

The vascular plants in Bukhansan Dulegil, Jingwan-dong Wetland and Bukhan-dong Cheolgeoji were listed 432 taxa (8.9% of all 4,881 taxa of vascular plants); 95 families, 274 genera, 345 species, 2 subspecies, 69 varieties and 16 forms. Divided into woody plants were 145 taxa (33.6%) and herbaceous plants were 287 taxa (66.4%). The Bukhansan Dulegil were listed (Myeongsang-gil 159 taxa, Huingureum-gil 227 taxa, Wooyiryeong-gil 216 taxa, Banghakdong-gil 139 taxa, Boru-gil 199 taxa), Jingwan-dong Wetland were listed 147 taxa and Bukhan-dong Cheolgeoji were listed 129 taxa. Based on the list of rare plants by Korea Forest Service and Korea National Arboretum were Viola albida Palibin (Violaceae), Iris minutiaurea Makino (Iridaceae) and Korea National Arboretum were endemic plants, Philadelphus schrenkii Rupr. var. schrenkii (Saxifragaceae), Weigela subsessilis L.H. Bailey (Caprifoliaceae). Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 22 taxa; Wisteria floribunda DC. for. floribunda in class IV, 3 taxa (Betula davurica Pall., Mukdenia rossii Koidz., Glechoma grandis Kuprian., etc.) in class III, 5 taxa (Acer triflorum Kom., Viola orientalis W. Becker, Heloniopsis koreana Fuse & Al., etc.) in class II and 13 taxa (Camptosorus sibiricus Rupr., Clematis patens C. Morren & Decne., Cirsium pendulum Fisch. ex DC., etc.) in class I. Based on the list of naturalized plants, 16 families, 35 genera, 37 species, 3 varieties, 1 form, total 41 taxa (Rumex crispus L., Abutilon theophrasti Medicus, Panicum dichotomiflorum Michx. etc.), naturalization rate was 9.5% of all 432 taxa of vascular plants and urbanization index was 13.3% of all 309 taxa of naturalized plants. Ecosystem disturbing wild plants were 5 taxa; Rumex acetocella L., Ambrosia artemisiifolia L., Ambrosia trifida L. var. trifida, Eupatorium rugosum Houtt., Aster pilosus Willd.