• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.93-97
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• 2011

Previously, we reported that the osmolarity conditions in the satellite region were affected CpG DNA methylation status while Pre-1 sequence was not affected CpG DNA methylation in pNT blastocyst stage. This study was conducted to investigate the DNA methylation status of repeat sequences in pig nuclear transfer (pNT) embryos produced under different osmolarity culture conditions. Control group of pNT embryos was cultured in PZM-3 for six days. Other two treatment groups of pNT embryos were cultured in modified PZM-3 with 138 mM NaCl or 0.05 M sucrose (mPZM-3, 320 mOsmol) for two days, and then cultured in PZM-3 (270 mOsmol) for four days. The DNA methylation status of the Pre-1 sequences in blastocysts was characterized using a bisulfite-sequencing method. Intriguingly, in the present study, we found the unique DNA methylation at several non-CpG sequences at the Pre-1 sequences in all groups. The non-CpG methylation was hypermethylated in all three groups, including in vivo group (86.90% of PZM-3; 83.87% of NaCl; 84.82% of sucrose; 90.94% of in vivo embryos). To determine whether certain non-CpG methylated sites were preferentially methylated, we also investigated the methylation degree of CpA, CpT and CpC. Excepting in vivo group, preference of methylation was CpT>CpC>CpA in all three groups investigated. These results indicate that DNA methylation of Pre-1 sequences was hypermethylated in CpG as well as non-CpG site, regardless modification of osmolarity in a culture media.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.8
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• pp.1620-1625
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• 2004

To advance fisheries, we set developmental directions of fishery information by grasping present situations and analyzing maritime & fisheries issues. We promote various policies through effective systematical information data bases, based on both control and utilization of oceanic resources. For these puposes, it is imperative that we set up fisheries communication networks. There are satellite assisted informational networks to assist fishing vessels with their marine based movements. However, there's no hope for poorly equipped fishermen to adopt this network because of extravagant network call charges. So we think that using existing SSB communication system is the best plan. We organize fishery communication network by HF SSB communication which doesn't have operational costs. We build wireless transmitting and receiving stations that are basic systems of informnation, and equip wireless data communication systems by the use of wireless communication network protocols in coastal stations. It is necessary that a fish boat has a terminal device for wireless data communication. In this research we can conclude that if we transmit the location of a fishing boat in-real time through GPS channels then we propose that some methods be formulated to able terminal devices on fishing boats to collect various types of information, such as meteorological and oceanic conditions.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.3
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• pp.249-258
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• 2010

This study seeks to improve NFRDI Serial Oceanographic observation (NSO) system which has been operated at current observation stations in the Korean Seas since 1961 and suggests the direction of NSO for practical use of Korean operational oceanographic system. For improvement, data handling by human after CTD (Conductivity-Temperature-Depth) observation on the deck, data transmission, data reception in the land station, and file storage into database need to be automated. Software development to execute QA/QC (Quality Assurance/Quality Control) of real-time oceanographic observation data and to transmit the data with conversion to appropriate format automatically will help to accomplish the automation. Inmarsat satellite telecommunication systems with which have already been equipped on board the current observation vessels can realize the real-time transmission of the data. For the near real-time data transmission, CDMA (Code Division Multiple Access) wireless telecommunication can provide efficient transmission in coastal area. Real-time QA/QC procedure after CTD observation will help to prevent errors which can be derived from various causes.

• Proceedings of the KSRS Conference
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• 2006.03a
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• pp.178-186
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• 2006

기상 수치예보는 (Numerical Weather Pridiction, NWP)는 바람, 기온, 등과 같은 기상요소의 시간 변화를 나타내는 물리방정식을 컴퓨터로 풀어 미래의 대기 상태를 예상하는 과학적인 방법으로 지구를 상세한 격자 2진부호(GRIdded Binary, 이하 GRIB)로 나누어 그 격자점에서의 값으로 대기 상태를 나타낸다. 지구 각지에서의 각종 관측자료를 기초로 격자점상의 현재값을 구한다. 대용량의 격자데이터는 이진형태이어서 컴퓨터, 서버 저장장치에서 동일형태 데이터로 존재한다. 우리나라 최초의 저궤도 관측 위성인 다목적 실용위성 KOMPSAT-1호(이하, 아리랑 위성1호)는 전자광학카메라(Electro Optical Camera, EOC)를 탑재하여 1999년 12월 21일에 발사된 이후 2006년 1월 현재까지 6여년간 성공적으로 임무를 수행, 7049여회의 영상을 획득하여 국가적으로 귀중한 자료로 활용하고 있다. 아리랑 위성1호는 일일 2-3회 EOC영상을 획득하고 있으며, 임무계획(Mission Planning)은 MP(Mission Planner)가 사용자로부터 자료를 수집하여 임무분석 및 계획 서브시스템(MAPS)에 의해 계산되어진 위성의 제도예측 데이터에 촬영하고자하는 목표지점 좌표를 입력하여 자동명령생성기(KSCG)에 의해 계산된 촬영 경사각도(Tilt)값을 위성에 전송하여 목표지역의 영상을 획득하게 된다. 위성영상 획득에 있어 고가의 위성을 운영하면서 기상의 상태를 정확히 예측하여 실패없이 유효한 영상을 획득하는 것이 무엇보다 중요하다. 본 논문에서는 효율적인 위성임무계획을 위한 기상수치예보 자료를 분석하여 앞으로 발사하게 될 고해상 카메라 탑제위성인 아리랑 위성2호와 3호에 적용하고자 한다. the sufficient excess reactivity to override this poisoning must be inserted, or its concentration is decreased sufficiently when its temporary shutdown is required. As ratter of fact, these have an important influence not only on reactor safety but also on economic aspect in operation. Considering these points in this study, the shutdown process was cptimized using the Pontryagin's maximum principle so that the shutdown mirth[d was improved as to restart the reactor to its fulpower at any time, but the xenon concentration did not excess the constrained allowable value during and after shutdown, at the same time all the control actions were completed within minimum time from beginning of the shutdown.및 12.36%, $101{\sim}200$일의 경우 12.78% 및 12.44%, 201일 이상의 경우 13.17% 및 11.30%로 201일 이상의 유기의 경우에만 대조구와 삭제 구간에 유의적인(p<0.05) 차이를 나타내었다.는 담수(淡水)에서 10%o의 해수(海水)

• Journal of the Korean Geographical Society
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• v.39 no.3
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• pp.344-359
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• 2004

This geographical study is to identify the reality of Nokdun island which was once settled and farmed by our ancestor, but currently the island is do facto under Russia's control. Various historical documents including old maps, current large scale maps and satellite images besides field work were used for full analysis. According to historical records, up until Chosun Dynasty, Nokdun island in the lower Tuman river was one of the frontier fortified base crossed the river for the northern defense as well as seasonal farming for the farmer of Kyounghung border area. Even though the island was unjustly lost to Russia by the Beijing Treaty in the late Chosun period, the whole area of Nokdun was occupied and farmed by sole Koreans until the deportation by Stalin was forcibly measured. In these days due to the change of Tumn river channel and new deposition by river it became very difficult to find the original shape of Nokdun island. However, through intensive field works based on historical records, the research team found out the existence of the old wet-dry fields, old rural farmhouse remnants, and small and medium size of millstones that are supporting the reality of the island. Especially, the team traced a mud fortress presumedly built in the early Chosun period. We believe it might allow to allocate the exact location of Nokdun island in near future.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2003.04a
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• pp.62-62
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• 2003

Landsat TM 영상을 이용, 명암차가 높은 산악 지역에 적용해왔던 알고리즘을 개선하여 비교적 명암차가 낮고 넓게 분포하는 충적층 지역의 선구조를 추출하는 알고리즘을 개발하였다. 수치지형모델(OEM)에 대하여 Local Enhancement 를 이용해서 평탄한 지역을 선정하여 이로부터 충적층을 추출하였다. Zevenbergen & Thorno's Method를 3×3 moving windowing을 통해서 최대 경사방향과 경사를 구해서 충적층을 지나는 선구조 요소를 추출하고 다시 Hough 변환을 이용해서 1차 선구조를 추출하였다 이를 이용하여 충적층의 직각방향의 지형단면의 경사를 유추해서 spline 보간법을 이용해 단면의 최저점을 구하고 이 구해진 점들을 다시 Hough 변환을 이용해서 최종 선구조를 추출하였다. 본 연구에서 사용한 알고리즘은 기존 알고리즘에서 사용된 소창문보다 훨씬 큰 충적층이 분포하는 지역의 지형 경사가 수렴하는 부분에 선구조가 뚜렷이 나타남을 볼 수 있다. 최대경사방향과 경사를 구해서 얻어진 1 차선구조와 최종 선구조에서 선구조 방향이 다소 차이를 보인다. 1 차 선구조의 수직방향 지형단면의 자료를 이용함에 있어, 지형 단면의 시작정과 끝지점을 임의적으로 결정하는 것이 아니라, 충적층을 가로질러 최고점까지 또는 다음 충적층이 나을 때까지의 자료를 이용해서 보간법을 사용하였고, 충적층의 넓이에 따라 보간할 자료량의 차이에 의한 오차가 발생할 수 있다. 넓은 충적층에서 선구조가 잘 추출되는 반면에 좁은 충적층이 분포하거나 계곡에 해당하는 지역에l서는 경사수렴부와 일치하지 않는 선구조가 추출되었다. 이는 향후 계속적으로 연구해서 보완되어야 할 것으로 사료된다.페클 잡영 제거 정도에 있어 다른 필터들과 큰 차이가 없지만 경계선보존지수는 다른 필터들에 비하여 가장 우수함을 확인할 수 있었다.rbon 탐식효율을 조사한 결과 B, D 및 E 분획에서 유의적인 효과를 나타내었다. 이상의 결과를 종합해볼 때, ${\beta}$-glucan은 고용량일 때 직접적으로 또는 $IFN-{\gamma}$ 존재시에는 저용량에서도 복강 큰 포식세로를 활성화시킬 뿐 아니라, 탐식효율도 높임으로써 면역기능을 증진 시키는 것으로 나타났고, 그 효과는 crude ${\beta}$-glucan의 추출조건에 따라 달라지는 것을 알 수 있었다.eveloped. Design concepts and control methods of a new crane will be introduced in this paper.and momentum balance was applied to the fluid field of bundle. while the movement of′ individual material was taken into account. The constitutive model relating the surface force and the deformation of bundle was introduced by considering a representative prodedure that stands for the bundle movement. Then a fundamental equations system could be simplified considering a steady state of the process. On the basi

• Korean Journal of Environment and Ecology
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• v.26 no.6
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• pp.952-959
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• 2012

This study examined habitat fragmentation that has occurred in Paju and Yeoncheon, the two border municipalities between North and South Korea in Gyeonggi-do (province) during the last 17 years using various landscape metrics. We 1) classified grass and agricultural habitats and forest habitats from two Landsat TM images collected in 1990 and 2007, and 2) compared the percentage of class area, patch density, mean patch area, and mean perimeter area ratio for the two habitat types between the two time points. Both types of habitats has been severely fragmented due to urban development in the last 17 years. The increased patch density and decreased mean habitat area are attributed to the construction of roads and railroads that separate a large habitat to many small pieces. The increased mean perimeter area ratio also indicates that the habitat fragmentation extended areas that are affected by the edge effect and so less suitable for interior species. A habitat conservation plan is urgently needed to minimize habitat fragmentation from developments that are expected to soon occur in the north and south Korean border.

• Research in Plant Disease
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• v.7 no.1
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• pp.1-7
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• 2001

An isolate of Cucumber mosaic cucumovirus(CMV) was isolated from Hydrangea macrophylla for. otaksa(Sieb. et Zucc. ) Wils. showing mosaic symptoms, and designated as Hm-CMV. Hm-CMV was characterized by the tests of host range, physical properties, serological properties, RNA and coat protein compositions, and reverse transcription and polymerase chain reaction (RT-PCR) analysis. Twelve species in 4 families were used in the host range test of Hm-CMV and could be differentiated from Y-CMV used as a control CMV by the ringspot and line pattern on inoculated leaves of several tobacco plants. Thevirus produced local lesions on inoculated leaves of Chenopodium amarticolor, C. quinoa and Vigna unguiculata. The physical properties of the virus were as follows; thermal inactivation point(TIP) was 60$\^{C}$, dilution end point (DEP) was 10$\^$-3/, and longevity in vitro (LIP) was 3∼4 days. Hm-CMV was serologically identical to Y-CMV. SDS-polyaciylamide gel electrophoresis(SDS-PAGE) showed one major protein band of about 28 kDa. In RNA or dsRNA analysis, Hm-CMV consisted of four RNA or dsRNA species, but satellite RNA was not detected. In RT-PCR using CMV-common primer and CMV subgroup I-specific primer, bothe amplified expected size of about 490 bp and 200 bp DNA fragments from Hm-CMV, respectively. Restriction enzyme analysis of the 490 bp RT-PCR products using EcoR I and Msp I showed that Hm-CMV belonged to CMV subgroup I. However, Hm-CMV could be differentiated from other CMV subgroup I isolates by RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP-PCR).

• Journal of Advanced Navigation Technology
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• v.16 no.1
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• pp.16-26
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• 2012

Pseudolites are ground-based transmitters that can be configured to emit GPS-like signals for enhancing the GPS by providing increased accuracy, integrity, and availability. However, a pseudolite (PL) can interfere with GPS satellite signals while it is transmitting or cause saturation to automatic gain control circuit. To solve these problems pulsing scheme is used, which transmits PL signal during a short period of time. In this paper the effect of the number of PL and pulsing scheme on the software GPS L1 and L2C signal acquisition performance is studied for the three pulsing schemes such as static pulsing, sweep pulsing, and pseudo random pulsing. For GPS L1 signal, static pulsing shows the best signal acquisition and tracking performance with one PL, and random pulsing shows the best performance with more than or equal to two PLs. For GPS L2C signal, all three pulsing schemes show the similar signal acquisition and tracking performance, but static pulsing shows a little better performance. For GPS L1 and L2C signals, software GPS receivers can do positioning with up to three PLs.

• Ocean and Polar Research
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• v.32 no.4
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• pp.337-350
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• 2010

This study conducted sedimentological and geophysical surveys for 3 years (2006-2008) in southern Gyeonggi Bay, Korea to elucidate temporal changes in subaqueous dune morphology on a sand ridge trending northeast to southwest that has been excavated by marine sand mining. The sand ridge (~20 m in height, ~2 km in width and 3~4 km in length) has a steep slope on the NW side and a gentle slope on the SE side, creating an asymmetric profile. Large (10~100 m in length) and very large (>100 m in length) dunes occurring on the SE side of the ridge show a northeastward asymmetrical shape, whereas dunes on the NW side destroyed by marine sand mining display a southwestward asymmetry. The comparison between Flemming (1988)'s correlation and the height-length correlation of this study indicates that tidal current and availability of sand sediment are major controlling factors to the development and maintenance of dunes. Depth and sedimentary characteristics (grain size) are not likely to be major controlling factors, but indirectly influence dune growth by hydrological and sedimentary processes. The length and the height of dunes decrease toward the southeastern trough away from the crest of the ridge. These features result from the decrease of tidal current and sediment availability. The length and the height of dunes on the southeast side decrease gradually over time. This is a result of the interaction between tidal current and the decrease in sediment availability due to sediment extraction by marine sand mining. Marine sand mining has destroyed the dunes directly, causing irregular shapes of shorter length and lower height. The coarse fraction of suspended sediments is transported and deposited very close to the sand pit. By contrast, relatively fine sediments are transported by the tidal current and deposited over a wide range by the settling-lag effect, resulting in a decrease of sediment grain size in the area where suspended sediments are deposited. In addition, marine sand mining, decreases the height of dunes. Therefore, morphological and sedimentological characteristics of dunes around the sand pits will be significantly changed by future sand mining activities.