This study was to test the relationship between regular physical exercise and physical fitness in nursing students. These subjects were 54 volunteers who agreed with intention of this study and were composed of 24 active group and 30 non-active group. The selected physical fitness tests were grip strength and back strength for measuring muscular strength, sargent jump and standing broad jump for measuring power, push-up, sit-up, and heart rate for measuring total endurance, reaction time and side step test for measuring agility, stick test and stork stand for measuring balance, and trunk flexion and trunk extension for measuring flexibility. The conclusion acquired was as follows : 1) Grip strength had significant difference between active group of $29.5{\pm}5.9kg$ and non-active group $25.4{\pm}7.2kg$(t=2.283, p=.027), and back strength between active group of $54.1{\pm}13.7kg$ and non-active group of $44.7{\pm}15.7kg$(t=2.310, p=.025), 2) Sargent jump was lower with non-active group($44.9{\pm}8.6cm$) than with active group($45.3{\pm}8.8cm$), however, was not significant(t=.182, p=.856). Standing broad jump was significantly lower with non-active group of $161.3{\pm}28.7cm$ than with active group of $191.4{\pm}27.0cm$(t=3.939, p=.000). 3) Push-up showed significant difference between active group of $25.3{\pm}12.3times$ and non-active group of $11.5{\pm}5.1times$(t=5.572, p=.000), and sit-up between active group of $21.8{\pm}7.4times$ and non-active group of $17.1{\pm}5.8times$(t=2.631, p=.011), and heart rate between actvie group of $110.5{\pm}12.8$beats/min and non-active group of $121.5{\pm}9.5$beats/min(t=-3.648, p=.001). 4) Reaction time was significantly higher with non-active group of $.300{\pm}.051sec$ than with active group of $.341{\pm}.041sec$(t=-3.285, p=.002). Side step test was lower with non-active group($8.8{\pm}1.1times$) than with active group($9.2{\pm}1.2times$), however, was not significant (t=1.309, p=.196). 5) Stick test showed not significant difference between active group of $25.8{\pm}14.8sec$ and non-active group of $30.7{\pm}17.9sec$(t=-1.058, p=.295), and stork stand between active group of $4.5{\pm}3.2sec$ and non-active group of $3.7{\pm}3.5sec$(t=.918, p=.363). 6) Trunk flexion came out not significant difference between active group of $14.2{\pm}5.0cm$ and non-active group of $15.8{\pm}7.3cm$(t=-.927, p=.358), and trunk extension between active group of $67.1{\pm}6.5cm$ and non-active group of $67.3{\pm}6.6cm$(t=-.140, p=.889). 7) Power was shown to be significantly related to total endurance(r=.717, p=.000 ; r-.739, p=.000). 8) Total endurance was shown to be significantly related to agility(r=-.752, p=.000 ; r=.684, p=.000 ; r=-.664, p=.000 ; r=.598, p=.002 ; r=.864, p=.000 ; r=-.536, p=.007). These results suggest that regular physical exercise is effective in promoting and maintaining physical fitness. As the further study, it is necessary to reinvestigate the effect with more refined design.