• 제목/요약/키워드: Sanghwang mushroom

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재배 상황버섯 추출물의 경구투여 급성독성 연구 (Acute Oral Toxicity Studies of Extract of Sanghwang Mushroom (Phellinus linteus))

  • 한용석;박순영;최병기;정세영
    • Biomolecules & Therapeutics
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    • 제9권1호
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    • pp.46-50
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    • 2001
  • The current study was performed to determine the acute oral toxicity of a crude extract of sanghwang mushroom (Phellinus linteus), in SD rats. 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at doses of 0, 500, 1,000, 2,000 mg/kg, respectively. After the treatment, clinical signs and body weight change, the food and water consumption were observed for 14 days. All animals survived during the study and did not show any clinical signs. Body weight gain showed no significant difference between the control and treated rats. However, body weight gain delayed in high dose group (2,000 mg/kg) on day 1~3 after administration. Another 5 rats of each sex were orally treated with a single dose of extract of sanghwang mushroom at dosages 4,000, 5,000 mg/kg respectively, but all animals survived during the study and did not show any clinical signs. It is suggested that LD$_{50}$ of extract of sanghwang mushroom by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats.s.

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인공재배 상황버섯 재배종 Phellinus linteus 와 P. baumii 자실체 추출물의 항산화활성과 폴리페놀 성분 비교 (Comparison of antioxidant activities and polyphenolic compounds of extracts from artificially cultivated Sanghwang mushroom species, Phellinus linteus and P. baumii)

  • 민경진;윤봉식;강희완
    • 한국버섯학회지
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    • 제18권1호
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    • pp.29-36
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    • 2020
  • P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline-6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다.

상황버섯 균사체를 이용한 양갱 제조 및 품질 특성 (Quality Characteristics of Yanggaeng Supplemented with Sanghwang Mushroom (Phellinus linteus) Mycelia)

  • 홍성수;정은경;김애정
    • 대한영양사협회학술지
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    • 제19권3호
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    • pp.253-264
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    • 2013
  • The principal objective of this study was to examine the quality characteristics of yanggaeng supplemented with powder derived from Sanghwang mushroom (Phellinus linteus) mycelia. We analyzed the potential of utilizing Phellinus linteus mycelia as a functional food material by estimating total polyphenol and flavonoid contents, electron-donating abilities, as well as antioxidative activities of the water and ethanol extracts of Sanghwang mushroom mycelia. The total phenol and flavonoid contents of ethanol extracts from Phellinus linteus mycelia were 0.69 mg/ml and 0.16 mg/ml, respectively, while the contents from the water extract of Phellinus linteus mycelia were 0.66 mg/ml and 0.22 mg/ml, respectively. The electron-donating abilities of ethanol and water extracts from Phellinus linteus mycelia were 88.64 and 90.29%, respectively. The ABTS radical scavenging activities of ethanol and water extracts from Phellinus linteus mycelia were 89.74 and 71.35%, respectively. In terms of color values, as the level of powder increased, the value of L (lightness) decreased, whereas those of a (redness) and b (yellowness) increased. In regard to the mechanical properties of the samples, we noted significant differences in hardness, springiness, chewiness, and gumminess (P<0.05). The results of the sensory evaluation showed that the score from SHM (Sanghwang mushroom mycelia) with 4% powder was significantly higher than other samples in terms of sweetness, color, taste, texture and overall quality (P<0.05). Taken together, the recommended level of Phellinus linteus mycelia powder in yanggaeng is 4% for optimal sensory characteristics.

Hangover relieving effect of Sanghwang mushroom mycelium cultured in germinated buckwheat

  • An, Yoo-Jin;Cho, Sung-Min;Kim, Min-Su;Moon, Hae-Hee;Park, Dong-Soo;Jeon, Nam-Gen;Lee, oungjae;Han, Chang-Hoon
    • 대한수의학회지
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    • 제57권3호
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    • pp.147-154
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    • 2017
  • The present study was performed to evaluate the hangover relieving effect of germinated buckwheat (GB) and Sanghwang mushroom mycelium cultured in GB (SGB). Both GB and SGB showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities and significantly increased (p < 0.001) aldehyde dehydrogenase (ALDH) activities; up to 140% increase at concentrations of $16{\mu}L/mL$. Locomotor activity test results from alcohol-SGB and alcohol-GB groups showed improved motor activities over that of the alcohol-water group at 90 min post-administration. Both alcohol-GB and alcohol-SGB groups had significantly reduced (p < 0.001) alcohol ($40.02{\pm}33.38{\mu}g/mL$, $66.01{\pm}22.04{\mu}g/mL$, respectively) and aldehyde ($5.72{\pm}0.47{\mu}g/mL$, $6.72{\pm}1.70{\mu}g/mL$, respectively) concentrations in blood compared to those in the alcohol-water group ($199.75{\pm}33.83{\mu}g/mL$, $50.43{\pm}13.88{\mu}g/mL$, respectively) at 90 min post-administration. Based on cDNA microarray analysis, expressions of ALDH genes ALDH1a7 and ALDH18a1 and cytochrome P450 (CY450) gene CYP4a30b were upregulated in the alcohol-GB and alcohol-SGB groups compared to levels in the control group. Overall, the results suggest that both GB and SGB have hangover relieving effects by reducing blood acetaldehyde levels. The molecular mechanisms may involve ALDH activation and upregulated expression of alcohol metabolism-related genes such as ALDH and CYP450.

상황버섯 균사체 추출물의 숙취해소 효과에 관한 연구 (Hangover relieving effect of Sanghwang mushroom mycelium extract)

  • 김민수;안유진;이재철;박가령;박동수;전남근;이영재;한창훈
    • 대한수의학회지
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    • 제56권4호
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    • pp.241-247
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    • 2016
  • This study was conducted to evaluate the hangover relieving effect of Sanghwang mushroom mycelium extract (SME). The extract showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging effect in a concentration-dependent manner and high antioxidant capacity ($56.67{\pm}1.77%$) when administered at $120{\mu}g/mL$. In addition, SME significantly increased (p < 0.005) the aldehyde dehydronase (ALDH) activity ($126.03{\pm}9.11%$) when applied at 8 or $16{\mu}L/mL$. A locomotor activity test showed that the alcohol-water treated group showed significantly decreased motor activity at 90 min post-administration. However, the alcohol-SME treated group showed a 20-fold higher motor activity than that observed in the alcohol-water treated group at 90 min post-administration. Blood was harvested from each mouse at 90 min post-administration, and both alcohol and aldehyde concentrations were measured. The alcohol-SME treated group showed significantly lower (p < 0.5) alcohol ($120.13{\pm}12.83{\mu}g/mL$) and aldehyde ($7.26{\pm}1.22{\mu}g/mL$) concentrations than the values observed in the alcohol-water treated group. These results suggest that the hangover relieving effect of SME results from increased ALDH activity, which reduces the aldehyde concentration in the blood.

상황버섯, Phellinus linteus 계통과 Phellinus baumii의 항 혈소판 응집 효과 (Inhibitory effects of Sanghwang mushrooms, Phellinus linteus strains and Phellinus baumii, on platelet aggregation)

  • 강희완;이만휘;여운형
    • 한국버섯학회지
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    • 제15권3호
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    • pp.124-128
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    • 2017
  • 국내 상황버섯 재배 종인 Phellinus baumii (PB, Jangsu sanghwang), P. linteus (PLK, Korea Sangwhang), 그리고 P. linteus HN00k9 (PLH) 자실체의 methanol 추출물의 혈소판 응집 억제와 ATP release 감소효과를 조사하였다. PB, PLK, PLH methanol 추출물을 전 처리하고 혈소판 응집촉진물인 collagen (2.5 ug/ml), ADP (10 uM), thrombin (0.1 U/ml) 처리에 50ug/ml에서 200 ug/ml 농도에서 50%에서 95%의 혈소판응집억제 효과가 나타났으며 ATP release 감소효과는 200 ug/ml 농도에서 ADP(10 uM) 처리 혈소판에서 60%에서 50%로 나타났으며 이는 Phospholipase C의 inhibitor인 U73122 (4 uM)과 calcium chelator인 EDTA (1 mM)에 비하여 20%이상 높게 나타났다. 본 결과로부터 상황버섯 PB, PLK, PLH 메탄올 추출물은 심혈관계질병을 예방 하는 유용한 소재로 활용 가능 할 것으로 사료 된다.

항암성과 향미가 개선된 재래식 버섯균사체메주의 제조 (Preparation of Mushroom Mycelia-cultured Traditional Meju with Enhanced Anticaricinogenicity and Sensory Quality)

  • 김영숙;박철우;김석종;박숙자;류충호;조현종;김정옥;임동길;하영래
    • 한국식품영양과학회지
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    • 제31권6호
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    • pp.986-993
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    • 2002
  • 항암성과 향미가 개선된 기능성 재래식 버섯균사체메주는 버섯균을 액체배양하여 재래식메주의 각 면에 5개씩 만든 구멍(1X3 cm)에 버섯균배양액을 메주 무게의 10%를 접종한후 $25^{\circ}C$에서 4주간 배양하여 제조하였다. 제조한 버섯균사체 메주 중 상황, 영지, 또는 신령버섯균사체메주가 항암성과 향미완화능이 우수하였다.

상황버섯 열수추출물의 항산화 활성과 식후 혈당 상승 억제 효과 (Antioxidant and Anti-hyperglycemic Effects of a Sanghwang Mushroom(Phellinus linteusau) Water Extract)

  • 최황용;하경수;조성훈;가은혜;장흥배;권영인
    • 한국식품영양학회지
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    • 제25권2호
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    • pp.239-245
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    • 2012
  • The inhibitory activities of a water extract of Sanghwang mushroom(Phellinus linteusau)(SWE) against ${\alpha}$-glucosidases were evaluated in this study. Inhibiting these enzymes involved in the absorption of disaccharides significantly decreases the postprandial increase in blood glucose level after a mixed carbohydrate diet. Oxygen radical absorbance capacity and 1,1-diphenyl-2-picryl hydrazyl scavenging activities of the SWE were evaluated to investigate the antioxidant activity of the SWE associated with complications of long-term diabetes. Furthermore, the postprandial blood glucose lowering effect of SWE was compared to a known type 2 diabetes drug($Acarbose^{(R)}$) in a Sprague-Dawley rat model. SWE significantly reduced the blood glucose increase after sucrose loading. These results suggest that SWE, which has high ${\alpha}$-glucosidase inhibitory activity and high antioxidant activities, has the potential to contribute to a useful dietary strategy for controlling postprandial hyperglycemia.

Phellinus linteus KACC 93057P, '한경상황버섯'인공재배 자실체 페놀추출물의 항산화 활성 및 화학적 동정 (Chemical identification and antioxidant activity of phenolic compounds extracted from the fruiting body of 'Hankyong Sanghwang', Phellinus linteus KACC 93057P)

  • 민경진;정이은;윤봉식;강희완
    • 한국버섯학회지
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    • 제16권4호
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    • pp.311-317
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    • 2018
  • 참나무 단목원목으로 재배한 P. linteus KACC93057P(PLHS)2년산 자실체 60%에탄올 추출물의 총 페놀함량은 $19.05{\pm}0.32mg\;GAE/g$로 다른 비교구 버섯 종에 비하여 4배에서 10배이상의 높게 나타났다. $125{\mu}g/mL$농도에서 DPPH와 ABTS 라디칼 소거능은 62%와 100%를 나타냈으며 FRAP 환원력은 $0.7{\mu}MFeSO_4/g$으로 비교 구 버섯종 보다 2에서 10배이상의 유효활성이 검출 되었다. PLHS 자실체 ethyl acetate분배 추출물의 HPLC분석에서 styrylpyrone 계열의 화합물 davallialactone, hispidin, hypholomine B와 phenylpropanoid 계열의 화합물인 caffeic acid가 동정 되었다.

재래식 버섯균사체 된장의 항종양성과 항돌연변이성 (Enhanced Antitumorigenicity and Antimutagenicity of Doenjang Prepared from Mushroom Mycelia-cultured Traditional Mejus)

  • 김석종;박철우;박숙자;김영숙;조현종;임동길;김정옥;이주희;하영래
    • 한국식품영양과학회지
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    • 제32권1호
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    • pp.143-148
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    • 2003
  • 재래식 버섯균사체메주로부터 제조된 재래식 버섯균사체 된장의 항종양성, 항산화성 및 항돌연변이성을 연구하였다. 항종양성 (S-180 세포로 유발한 mouse ascites cancer) 에서는 느타리, 영지, 상황, 신령 버섯균사체 된장이 각각 33, 30, 26, 25% 억제하여 재래식 된장(11%)에 비해 매우 우수한 항종양성을 나타내었다. 버섯균사체 된장은 S. typhimurium TA98에 대한 IQ와 AFB$_1$에 의한 돌연변이성을 각각 39.7~46.1%, 38.0~39.7% 억제하여 대조된장(32.0, 33.2%)에 비해 항돌연변이성이 우수하였다. S. fyphimurium TA 100에 대해서는 IQ와 AFB$_1$의 돌연변이성을 각각 31.9~35.6%, 24.9~30.4% 억제하여 대조된장(27.5, 22.5%)에 비해 항돌연변이성이 좋았다. 항산화성 역시 버섯균사체된장이 대조된장에 비해 효과가 우수하였다 관련 물질로서 $\beta$-D-g1ucan 함량은 버섯균사체된장의 경우 재래식 된장에 비하여 3~8배, 총 Phenol성 화합물과 isoflavone 함량은 느타리버섯균사체된장과 신령버섯균사체가 가장 높았다.