• 제목/요약/키워드: Sample pre-treatment

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시료 중 잔류 항생제 분석 방법: II. 액상 시료 전처리 방법 (Determination of Antibiotic Residues: II. Extraction and Clean-up Methods for Liquid Samples_A Review)

  • 김찬식;류홍덕;정유진;김용석;류덕희
    • 한국물환경학회지
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    • 제32권6호
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    • pp.628-648
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    • 2016
  • Increased attention has been paid to the presence of veterinary antibiotics in various environmental matrices due to their toxicological behavior in the ecosystem and development of antibiotic-resistant strains of pathogenic bacteria. In the this review, 37 target antimicrobials were selected based on annual sales of antibiotics for livestock in South Korea 2014. Also, extraction and clean-up methods for the determination of the antibiotic residues in liquid samples including water, milk, and honey were comprehensively reviewed in the literature. Solid-phase extraction (SPE) was commonly used as a pre-treatment method for the samples. Most of the analytes were extracted in acidic conditions (2.5~4.0) except for aminoglycosides, which were extracted in neutral conditions (7.0~8.0). ${\beta}-Lactams$ showed the highest recoveries in neutral pH due to their degradation characteristics in acidic media. Starta-X, Oasis HLB, and Oasis MCX were frequently applied as an SPE cartridge and Oasis HLB showed the highest recoveries for the majority of antibiotic classes. The homogenized honey and milk were extracted by mixing with acids for deproteinization. Solids and other interfering substances in the extract were eliminated by centrifugation followed by membrane filtration or SPE before injection into HPLC.

Effects of Mechanically Activated Milling and Calcination Process on the Phase Stability and Particle Morphology of Monoclinic Zirconia Synthesized by Hydrolysis of ZrOCl2 Solution

  • Lee, Young-Geun;Ur, Soon-Chul;Mahmud, Iqbal;Yoon, Man-Soon
    • 한국재료학회지
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    • 제23권10호
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    • pp.543-549
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    • 2013
  • The purpose of this paper was to investigate the effect of a high-energy milling (HEM) process on the particle morphology and the correlation between a thermal treatment and tetragonal/monoclinic nanostructured zirconia powders obtained by a precipitation process. To eliminate chloride residue ions from hydrous zirconia, a modified washing method was used. It was found that the used washing method was effective in removing the chloride from the precipitated gel. In order to investigate the effect of a pre-milling process on the particle morphology of the precipitate, dried $Zr(OH)_4$ was milled using a HEM machine with distilled water. The particle size of the $Zr(OH)_4$ powder exposed to HEM reduced to 100~150 nm, whereas that of fresh $Zr(OH)_4$ powder without a pre-milling process had a large and irregular size of 100 nm~1.5 ${\mu}m$. Additionally, modified heat treatment process was proposed to achieve nano-sized zirconia having a pure monoclinic phase. It was evident that two-step calcining process was effective in perfectly eliminating the tetragonal phase, having a small average particle of ~100 nm with good uniformity compared to the sample calcined by a single-step process, showing a large average particle size of ~300 nm with an irregular particle shape and a broad particle size distribution. The modified method is considered to be a promising process for nano-sized zirconia having a fully monoclinic phase.

Influence of late removal after treatment on the removal torque of microimplants

  • Kim, Ho-Jin;Park, Hyo-Sang
    • 대한치과교정학회지
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    • 제52권3호
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    • pp.201-209
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    • 2022
  • Objective: To compare the removal torque of microimplants upon post-use removal and post-retention removal and to assess the influencing factors. Methods: The sample group included 241 patients (age, 30.25 ± 12.2 years) with 568 microimplants. They were divided into the post-use (microimplants removed immediately after use or treatment) and post-retention (microimplants removed during the retention period) removal groups. The removal torque in both groups was assessed according to sex, age, placement site and method, and microimplant size. Pearson correlation and multiple linear regression analyses were performed for evaluating variables influencing the removal torque. Results: The mean period of total in-bone stay of microimplants in the post-retention removal group (1,237 days) was approximately two times longer than that in the post-use removal group (656.28 days). The removal torques in the post-retention removal group (range, 4-5 N cm) were also higher than those in the post-use removal group. The mandible and pre-drilling groups demonstrated higher placement and removal torques than did the maxilla and no-drilling groups, respectively. In the no-drilling post-use removal group, the placement torque and microimplant length positively correlated with the removal torque. In the post-retention removal group, unloading in-bone stay period and microimplant diameter positively correlated with the removal torque in the no-drilling and pre-drilling methods, respectively. Conclusions: The removal torques differed according to the orthodontic loading and removal time of microimplants. With prolonged retention of microimplants inserted using the no-drilling method, the removal torque was clinically acceptable and positively correlated with the unloading in-bone stay period.

도시 저소득층 주부의 영양태도, 영양지식도 및 식생활을 통해서 본 영양 교육의 효과 (Effect of Nutrition Education Program on Nutrition Behavior of Housewives in a Low-Income Urbon Area)

  • 강명희
    • Journal of Nutrition and Health
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    • 제25권2호
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    • pp.162-178
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    • 1992
  • This study evaluated the effectiveness of affected-based program in changing the nutrition attitudes and behavior of a group of low-income housewives in a urban area near industrial complex. using pre- post- and one month retention test measures of nutrition attitudes knowledge and nutrition behavior. The sample consisted of treatment group(n=58) and control group(n=68, 66 or 61) The treatment group participated in a four-week nutrition course of eight hours offered by faculty members of department of Food and Nutition in Han Nam University. At the conclusion of the program the respondents had improved nutrition attitudes increa-sed nutrition knowledge and improved dietary diversity and dietary quality scores. Nutrition attitude scores rose significnatly from the pretest to post-test for the treatment group only However this improvement was not maintained one month after education. Nutrition know-ledge score of the treatment group increased significnatly from the pretest to posttest and pretest to retention test. Nutrition behavior was measured by food practice dietary diversity and dietary quality scores, The mean food practice score dietary diversity and dietary quality scores were signifi-cantly increased from pretest to posttest for the treatment group. Only dietary diversity scores was maintained from posttest for the retention test for the treatment group. There was a good correlation between nutrition knowlege and attitude scores at the pretest. However no significant corrlation was found between nutrition knowledge and attitude scores at the posttest. At the pretest nutrition knowledge or attitude and behavior were not correlated bu there were significant correations between nutrition knowledge or attitude and behavior at the posttest. It was concluded that a four-week nutrition education program of eight hours duration for the target people can lead to ac hange in dietary behaviors as well as nutrition knowledge and attitude.

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중장년 남성 급성심근경색증 환자의 심혈관위험인자 인식 및 치료추구 장애요인 (Perceptions of Barriers to Cardiovascular Risk Factors and Decision to Seek Treatment among Middle-aged Men with Acute Myocardial Infarction)

  • 황선영;권영란;김애리
    • 성인간호학회지
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    • 제22권5호
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    • pp.537-551
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    • 2010
  • Purpose: This study was designed to identify meaningful themes related to the recognition of lifestyle risk factors and barriers in seeking treatment following an acute event of first-time acute myocardial infarction. Methods: A methodological mixed method of thematic content analysis and a quantitative analysis was used. The sample consisted of 120 male patients < 65 years of age who agreed to be in the study were interviewed using a semistructured during 2008-2009. Data were analyzed according to the procedure of thematic content analysis and the meaningful themes were coded into SPSS data for quantitative analysis. Results: Pre-hospital delay greater than three hours reported by 58.3% (n=70) of the sample and similarly 63.3% had no recognition about their symptoms as cardiac in origin. The mean number of risk factors was $3.9{\pm}1.8$ out of 11 when lifestyle and psychosocial factors were included. From the interview data among the 70 patients delayed greater than three hours, thirty-five themes categorized into 12 main themes influenced the delayed decision which was identified according to personal-cognitive, socio-cultural, and contextual factors. Conclusion: Health care providers should consider these themes in designing individual interventions to make lifestyle changes and to facilitate more prompt decisions to seek care.

Analysis of midpalatal miniscrew-assisted maxillary molar distalization patterns with simultaneous use of fixed appliances: A preliminary study

  • Mah, Su-Jung;Kim, Ji-Eun;Ahn, Eun Jin;Nam, Jong-Hyun;Kim, Ji-Young;Kang, Yoon-Goo
    • 대한치과교정학회지
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    • 제46권1호
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    • pp.55-61
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    • 2016
  • Skeletal anchorage-assisted upper molar distalization has become one of the standard treatment modalities for the correction of Class II malocclusion. The purpose of this study was to analyze maxillary molar movement patterns according to appliance design, with the simultaneous use of buccal fixed orthodontic appliances. The authors devised two distinct types of midpalatal miniscrew-assisted maxillary molar distalizers, a lingual arch type and a pendulum type. Fourteen patients treated with one of the two types of distalizers were enrolled in the study, and the patterns of tooth movement associated with each type were compared. Pre- and post-treatment lateral cephalograms were analyzed. The lingual arch type was associated with relatively bodily upper molar distalization, while the pendulum type was associated with distal tipping with intrusion of the upper molar. Clinicians should be aware of the expected tooth movement associated with each appliance design. Further well designed studies with larger sample sizes are required.

Low Income and Rural County of Residence Increase Mortality from Bone and Joint Sarcomas

  • Cheung, Min Rex
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권9호
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    • pp.5043-5047
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    • 2013
  • Background: This is a part of a larger effort to characterize the effects on socio-economic factors (SEFs) on cancer outcome. Surveillance, Epidemiology and End Result (SEER) bone and joint sarcoma (BJS) data were used to identify potential disparities in cause specific survival (CSS). Materials and Methods: This study analyzed SEFs in conjunction with biologic and treatment factors. Absolute BJS specific risks were calculated and the areas under the receiver operating characteristic (ROC) curve were computed for predictors. Actuarial survival analysis was performed with Kaplan-Meier method. Kolmogorov-Smirnov's 2-sample test was used to for comparing two survival curves. Cox proportional hazard model was used for multivariate analysis. Results: There were 13501 patients diagnosed BJS from 1973 to 2009. The mean follow up time (SD) was 75.6 (90.1) months. Staging was the highest predictive factor of outcome (ROC area of 0.68). SEER stage, histology, primary site and sex were highly significant pre-treatment predictors of CSS. Under multivariate analysis, patients living in low income neighborhoods and rural areas had a 2% and 5% disadvantage in cause specific survival respectively. Conclusions: This study has found 2-5% decrement of CSS of BJS due to SEFs. These data may be used to generate testable hypothesis for future clinical trials to eliminate BJS outcome disparities.

The Effect of Peer Scaffolding on College Students' Writing Skills in EFL

  • Wooyoung Kim
    • International Journal of Advanced Culture Technology
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    • 제11권3호
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    • pp.156-162
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    • 2023
  • The purpose of this study was to examine the effects of peer scaffolding on the writing fluency of English language learners. This study was intended to confirm that peer scaffolding in English as a foreign language (EFL) writing classes can improve students' English proficiency. An analysis of 20 EFL learners studying at a university in Gyeonggi Province was conducted based on the English Proficiency Test. In this study, 20 intermediate learners with similar proficiency levels were included in the sample. Randomly, 10 students were designated as members of the control group, and 10 students were designated as members of the experimental group. In the experimental group, students practiced essay writing, while a skilled student provided scaffolding for a less skilled student. A variety of tools were used to gather data, including tests, questionnaires, and interviews Statistical analyses of quantitative data were conducted using t-tests for independent samples, whereas analyses of qualitative data were conducted based on themes. Pre-test results indicated a significant value of sig. =0.87, which was higher than α = 0.05. According to the results of this study, the writing performance of both experimental and control groups of students was equal and homogeneous prior to treatment. However, there were significant differences between the writing of students in the two groups after the completion of the program. Due to the post-test analysis of the writing test, the test resulted in a sig. =0 .043, a value lower than α = 0.05. As a result, the experimental group participants showed a marked improvement in their writing abilities after treatment.

고형연료제품의 유해중금속 분석을 위한 마이크로파 산 분해법의 개선 (Improvement of Acid Digestion Method by Microwave for Hazardous Heavy Metal Analysis of Solid Refuse Fuel)

  • 양원석;박호연;강준구;이영진;이영기;윤영욱;전태완
    • 한국폐기물자원순환학회지
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    • 제35권7호
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    • pp.616-626
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    • 2018
  • The quality standards of solid refuse fuel (SRF) define the values for 12 physico-chemical properties, including moisture, lower heating value, and metal compounds, according to Article 20 of the Enforcement Rules of the Act on Resource Saving and Recycling Promotion. These parameters are evaluated via various SRF Quality Test Methods, but problems related to the heavy metal content have been observed in the microwave acid digestion method. Therefore, these methods and their applicability need improvement. In this study, the appropriate testing conditions were derived by varying the parameters of microwave acid digestion, such as microwave power and pre-treatment time. The pre-treatment of SRF as a function of the microwave power revealed an incomplete decomposition of the sample at 600 W, and the heavy metal content analysis was difficult to perform under 9 mL of nitric acid and 3 mL of hydrochloric acid. The experiments with the reference materials under nitric acid at 600 W lasted 30 minutes, and 1,000 W for 20 or 30 minutes were considered optimal conditions. The results confirmed that a mixture of SRF and an acid would take about 20 minutes to reach $180^{\circ}C$, requiring at least 30 minutes of pre-treatment. The accuracy was within 30% of the standard deviation, with a precision of 70 ~ 130% of the heavy metal recovery rate. By applying these conditions to SRF, the results for each condition were not significantly different and the heavy metal standards for As, Pb, Cd, and Cr were satisfied.

고병원성 바이러스 검사를 위한 분자진단검사실 구축 (Designing a Molecular Diagnostic Laboratory for Testing Highly Pathogenic Viruses)

  • 정태원;정재영;김성현;김영권
    • 대한임상검사과학회지
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    • 제53권2호
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    • pp.143-150
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    • 2021
  • 최근 SARS-CoV-2를 포함한 신종 및 변종 고병원성 바이러스의 확산과 이를 확진하기 위한 진단검사의 수요가 증가함에 따라 분자유전검사실 구축 시 필요한 공간별 기능을 조사하고 임상병리사의 안전성 확보를 위한 시설 지침을 마련하고자 하였다. 검사과정 중 검체 전처리 및 핵산 증폭 전실 및 후실 분리와 함께 음압설비가 필요하며, 핵산 증폭 전 공간을 분리해 단방향 작업 흐름을 고려해 공간 구성을 하여야 한다. 검사 진행과정 중 검체 전처리, 핵산 증폭 전 단계에서 실 분리와 함께 음압시설이 필요하며, 공간구성은 핵산증폭 전실 및 후실 공간을 분리하고 검사진행 방향을 단방향 검사흐름(unidirectional work flow)을 고려하여 계획하여야 한다. 안전관리 시설은 생물학적 안전 기준 2등급으로 지정하고 그에 따른 안전보호구를 배치하고, 위험물을 취급하는 경우 생물학적 안전상자 내부에서 진행해야 하며, 전염성 오염물의 폐기를 위한 멸균기가 필요하다. 국내 분자유전검사실 사례의 공통점은 검체 전처리공간을 -2.5 Pa 이하의 음압으로 유지하고 있으며, 검사과정상 전처리와 시약준비 공간에 대해서는 다른 공간에서 진행하고 있었다. 본 연구는 검사과정의 세부흐름과 공간별 기능을 분리하고 안전관리 기준 적용한 검사실 구축 방향을 제시하였고, 임시시설과 결핵검사실을 변경하여 사용하는 사례를 소개함으로써 더욱 효율적인 방향을 모색할 수 있는 자료로 의의가 있다고 하겠다.