• Title/Summary/Keyword: Salt-tolerance

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Different Physiological Response to Salt in Salt Tolerant Rice Mutants Induced by Gamma-Mutagenesis

  • Jang, Duk-Soo;Song, Mira;Kim, Sun-hee;Kim, Jin-Baek;Kim, Sang Hoon;Ha, Bo-Keun;Kang, Si-Yong;Kim, Wook;Kim, Dong Sub
    • Journal of Radiation Industry
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    • v.5 no.3
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    • pp.259-266
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    • 2011
  • When plants undergo stress, Reactive oxygen species (ROS) which remove bad elements such as mildew and virus is activated in plant body. However, if ROS is excessively increased, plant will be harmed itself by destruction of cell and signal system and phenomenon of lipid peroxidation. In order to identify content of lipid peroxidation and activity of some enzymes scavenging ROS, phenotypical and physiological analysis was performed with two mutant lines, Till-II-877 and Till-II-894, comparing with cv. Dongan (WT). In phenotype analysis, two mutant lines give to well-conditioned growth better than WT in since 5 days after salt treatment. In enzyme activities, there was a modest difference in the content of catalase (CAT) and peroxidase (POD) between Till-II-877 and Till-II-894, two mutant lines showed high levels in CAT contents than WT. However, they express low levels in POD contents. In MDA analysis, the content of Till-II-877 was higher than that of WT, but Till-II-894 was lower. This result indicates that two mutants have different mechanism against salt stress.

Physiological Response to Salinity Stress of Japonica/Indica Lines Tolerant to Salt at Seedling Stage

  • Ko, Jong-Cheol;Lee, Kyu-Seong;Kim, Ki-Young;Choi, Weon-Young;Kim, Bo-Kyung;Shin, Woon-Cheol;Ko, Jae-Kwon;Yum, Song-Joong
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.391-398
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    • 2011
  • Physiological responses to salinity stress were evaluated in six rice genotypes differing in their tolerance to salinity at the seedling stage. Susceptible genotypes ('Dongjingbyeo', 'Hwayeongbyeo', and 'IR29') showed salt injury symptoms (mean 8.8) and higher visual score under salt stress than that of tolerant ones ('Pokkali', 'IR74009', and 'IR73571'). As salinity affects growth and physiological parameters, the six genotypes thus showed significant reduction because of salt stress. Tolerant Japonica/Indica bred lines ('IR74009', 'IR73571') showed lower reduction, 33.9%, 34.5%, and 50%, respectively, in plant seedling height, dry shoot weight and dry root weight than those of the susceptible Japonica varieties ('Dongjingbyeo', 'Hwayeongbyeo'), and the highest reduction under salt stress was observed in dry root weight, followed by dry shoot weight and seedling height, respectively. Shoot $Na^+$ concentration of IR74099 and IR73571 was lower than that of the susceptible varieties, 'Dongjinbyeo' and 'Hwayeongbyeo'. There were no significant differences among genotypes in root $Na^+$ concentration. Shoot $K^+$ concentration showed a reverse tendency compared to shoot $Na^+$ concentration. IR74009 and IR73571 had considerably lower ratio compared to 'Dongjinbyeo' and 'Hwayeongbyeo' in $Na^+/K^+$ ratio of their shoot and was not different the tolerant check, 'Pokkali'.

Isolation of salt-tolerant bacteria from rhizosphere and rhizoplane of halophyte plant Suaeda japonica in Gochang·Buan tidal flat (고창·부안 갯벌에 자생하는 염생식물 칠면초 근권 및 근면으로부터 내 염성 세균 분리와 그 특성화)

  • Ki, Min-Gyu;Lee, Hyeri;Cho, Ahyeon;Unno, Tatsuya;Lee, Ji-Hoon
    • Journal of Applied Biological Chemistry
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    • v.60 no.2
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    • pp.125-131
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    • 2017
  • Nine strains of high concentrations of salt-tolerant bacteria were isolated from the rhizosphere and rhizoplane of the halophyte plant Suaeda japonica grown in Gochang Buan tidal flat. The isolated bacteria were classified as genera Vibrio (strains JRS-1, -2, -3, -4, and -5, and JRL-1 and -4) and Bacillus (strains JRL-2 and -3) based on the 16S rRNA gene sequence similarity. The optical growth condition for salt concentration was examined on the selected, representative strains. Strain JRS-1 with the closest relative of Vibrio neocaledonicus showed the highest growth rate at the total salt concentration of 6% among the incubation conditions of 3-8% salt concentrations. Strain JRL-2 with the closest relative of Bacillus thuringiensis showed the tendency that growth rate increased with increasing salt concentrations and the maximum growth rate at 7% of the total salt concentration. The isolated bacteria showed salt-resistances to higher salt concentrations than their habitat soils with 3%. In addition, we identified evidences of potentially plant interaction-relevant enzymatic activities, from utilization of some substrates rich in plants, such as triglyceride, ${\rho}$-nitrophenyl-${\alpha}$,$\text\tiny{D}$-glucoside, and ${\rho}$-nitrophenyl-${\beta}$,$\text\tiny{D}$-glucoside.

The Desalinization Effects by Corn as a Cleaning Crop and Its Physiological Characteristics in Salt Accumulated Soil of the Plastic Film House Cultivation (염류집적 시설재배온실 토양에서의 Cleaning crop인 옥수수 생리적 특성과 제염효과)

  • Yoon Byeong-Sung;Jin Cheng-Wu;Cho Dong-Ha
    • Korean Journal of Organic Agriculture
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    • v.14 no.2
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    • pp.179-189
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    • 2006
  • After examining the difference in the photosynthesis rate of corn according to the planting distance, the distance of $40{\times}40cm$ showed the lowest rate by 23 days after transplanting (May 31); however, there was no significant difference in the photosynthesis rate due to increased salt tolerance in the plant as time went by. As for the difference in growth features of a plant, the planting distance of $40{\times}40cm$ showed a growing disorder due to the influence of salt by 23 days after trans-plantation (May 31); however, there was a desirable growth as time went by. For the difference in the salt content within a plant, the planting distance of $40{\times}40cm$ tended to be higher than other planting distances, and the K+ content is much higher than other kinds of salt after examining the difference in salt absorption. As for the correlation between saline components within a plant, there was a significant negative correlation among $K^{+},\;Ca^{2+},\;and\;Na^{+}$ while there was a significant positive correlation among $Mg^{2+},\;Ca^{2+}$, and Na+. After examining the effectiveness of salt removal from soil according to com cultivation, the planting distance of $30{\times}30cm$ showed a remarkable decrease by 37 days, compared with 23 days, after transplantation in $K^{+}$ by 28%, $Ca^{2+}$ by 36.6%, $Mg^{2+}$ by 30.6%, and $Na^{+}$ by 22.9%. And the salt content is higher in surface soil than in subsoil.

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The Responses of Antioxidative Enzymes and Salt Tolerance of Atriplex gmelini (Atriplex gmelini(가는갯능쟁이)의 내염성과 항산화 효소 반응)

  • 배정진;윤호성;추연식;송승달
    • The Korean Journal of Ecology
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    • v.26 no.5
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    • pp.273-280
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    • 2003
  • Saline conditions invoke oxidative stress attributed to the overproduction of reactive oxygen species (ROS). Changes in quantum efficiency and antioxidative enzyme activity upon salt treatment were examined in a salt-tolerant plant, Atriplex gmelini, to test the hypothesis that salt tolerance of A. gmelini is due to the increased activity of antioxidative enzymes. A. gmelini showed optimum growth at 100 mM NaCl producing 116% of the shoot dry weight over control plants in 0 mM NaCl treatment. Healthy growth persisted up to 300 mM NaCl treatment maintaining normal internal water content and dry weight. No photochemical stress or damages on antioxidative defense system was obvious in plants of 2 and 4 day salt treatment which was indicated by increased quantum efficiency (Fv/Fm value), decreased stress index (Fo/Fm value), and increased activity of antioxidative enzymes such as SOD, APX, GR. However, the plants treated with 400 mM NaCl showed decrease in growth and in antioxidative enzyme activity although the enzyme activity was still higher than that of the 0 mM NaCl treated plants (l31%, 114%, and 134% of the SOD, APX, and GR activity, respectively). Interestingly, another important antioridative enzyme that scavenges H₂O₂ in plant cells, CAT, showed rapid decrease in its activity as salt concentration increased; 38%, 22%, 15% of the 0 mM NaCl treated plants at 200, 300, 400 mM NaCl treatments, respectively. It appears that the enzymes in ascorbate-glutathione cycle such as APX and GR play the major roles in scavenging ROS produced by salt stress in A. gmelini. After 6 days of salt treatment, the damage in photochemical and antioxidative defense system was indicated by decreased Fv/Fm value and increased Fo/Fm value. A. gmelini appears to cope with short term salt treatment by enhanced activity of the antioxidative defense system, whereas long term stress invoke oxidative stress by increased ROS due to the damages in photochemical and antioxidative system.

Characteristics of Salt Tolerance in Tree Species(I) - Relationship between Tree Species Distribution and Soil Salt Concentration in East Coastal Forest - (수종간(樹種間)의 내염력(耐塩力) 특성(特性)에 관(關)한 연구(硏究)(I) - 동해안림(東海岸林)의 수종분포(樹種分布)와 염분농도(塩分濃度)와의 관계(關係) -)

  • Choi, Moon Gil
    • Journal of Korean Society of Forest Science
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    • v.73 no.1
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    • pp.1-8
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    • 1986
  • Relationship between distribution of tree species and salt concentration in soil was studied in order to understand the salt tolerance of tree species in the middle part of Korean east coast and its results were as follows; 1) The tree species in the area mostly consist of Pinus thunbergii, Pinus densiflora, Rosa rugosa, Lespedeza bicolor, Amorpha fruticosa, Quercus dentata, Rhododendron mucronulatum, Rohinia pseudoacacia and others. 2) Pinus thunbergii was dominant species and Rosa rugosa gradually disappeared at the distance from the beach line to 200 meters toward inland. Pinus thunbergii tended to disappear gradually and Pinus densiflora was dominant at the distance from 200 meters to 300 meters inland. 3) Pinus thunbergii was dominant below 50 meters in altitude while Pinus densiflora was dominant above 50 meters. 4) NaCl content tended to decrease as proceeding to inland, higher altitude and to shallow depth of soil. 5) Change in pH in terms of distance toward inland and altitude was not significant, but change in pH along soil depth was conspicuously decreasing from pH 7.0 of top soil to pH 6.5 50cm below. 6) Pinus thunbergii was densely distributed to the area where soil NaCl content was higher than 100 vpm, while Pinus densiflora dominated the area of less than 100 vpm. Rosa rugosa was shown to dominate the area of over 100 vpm soil NaCl content. 7) NaCl content in tree tended to decrease, as proceeding to inland, rapidly within 150 meters distance from the beach line and gradually at further distances. NaCl content in leaf was about 600 ppm, branch 350ppm and root 250 ppm.

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Salt tolerance and proline accumulation of potato (Solanum tuberosum L.) in vitro plants to NaCl treatment (NaCl 처리에 따른 감자 (Solanum tuberosum L.) 기내 식물체의 내염성 및 Proline 함량 변화)

  • Im, Ju Sung;Cho, Ji Hong;Cho, Kwang Soo;Chang, Dong Chil;Jin, Yong Ik;Yu, Hong Seob;Cheun, Chung Gi;Kim, Mee Ok;Han, Da Som;Lee, Seul Ki;Kim, Wha Yeong
    • Journal of Plant Biotechnology
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    • v.42 no.2
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    • pp.129-134
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    • 2015
  • In order to compare salt tolerance among potato cultivars (Solanum tuberosum L.), in vitro plants of each cultivar were cultured on the Murashige and Skoog's medium containing different levels of NaCl (0, 75, 150, and 225 mM). The suitable level of NaCl for a comparison of the shoot and root growth between potato cultivars was 75mM. Shoot length and weight were better in 'Dejima', 'Superior', 'Jayoung', and 'Haryeong' than in other cultivars such as 'Goun' and 'Atlantic'. Normal root growth was observed in 'Seohong', 'Superior', and 'Haryeong', while 'Goun', 'Atlantic', 'Dejima', 'Jowon', and 'Chuback' showed no root. Proline contents in all the cultivars increased as affected by NaCl levels, then the increase amount was fewer in the cultivars showed a better growth in both shoot and root than in others showed a poor growth. As a result of this study, 'Superior' and 'Haryeong' were determined as salt tolerant cultivars and therefore using these cultivars it would be possible to potato production at salt arable land such as reclaimed tidal.

Evaluation of Salt Tolerance of Liriope platyphylla and Pachysandra terminalis to Deicing Salt (CaCl2) Concentration in Winter (겨울철 제설제(CaCl2)농도처리에 따른 맥문동과 수호초의 내염성 평가)

  • Ju, Jin-Hee;Hui, Xu;Park, Ji-Yeon;Choi, Eun-Young;Yoon, Yong-Han
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.651-657
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    • 2016
  • It is important to know know deleterious impact of deicing salt on plants for guidelines of planting along roads. The aim of this study was to determine the effect of calcium chloride ($CaCl_2$) on the growth and physiological characteristics of Liriope platyphylla and Pachysandra terminalis. The plants were grown from November of 2015 to March of 2016 in pots containing growing media with $CaCl_2$ at 0% (Control), 0.5%, 1.0%, 3.0%, and 5.0% (based on the weight). While plant growth and photosynthetic activity were significantly decreased in both plant species grown on the media with $CaCl_2$, the degree of sensitivity to $CaCl_2$ differed. The plant growth of Liriope platyphylla was considerably injured under higher than 3.0% of $CaCl_2$, whereas Pachysandra terminalis was all dead under higher than 1.0% of $CaCl_2$. This results indicate that Liriope platyphylla has higher degree of tolerance to the deicing salt than Pachysandra terminalis.

Characteristics of Salt Tolerance in Woody Species (III) -Effects of Salt Treatment on Physiological Responses in the Leaves of Several Tree Species- (수종간(樹種間)의 내염력특성(耐鹽力特性)에 관(關)한 연구(硏究)(III) - 몇 수종(樹種)에 대(對)한 염분처리(鹽分處理)가 엽(葉)의 생리적(生理的) 반응(反應)에 미치는 영향(影響) -)

  • Choi, Moon Gil
    • Journal of Korean Society of Forest Science
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    • v.77 no.4
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    • pp.371-381
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    • 1988
  • The purpose of this study was to investigate the physiological response to salt treatments in the leaves of several tree species. The results obtained were as follows : 1. The water potential of tree leaves damaged with various salt concentrations did not change nearly for 10 hours after treatment. As time elapsed after treatment, however, the higher salt concentration in soils, the higher leaf water potential was observed. 2. Leaf water potential of species intolerant to salt was higher than tolerant species due to the severe dehydration from cells. 3. According to the water relation parameters obtained from P-V curves, the values of ${\pi}_{\sigma}$ and ${\pi}_{\rho}$ in the damaged leaves were higher, but those of $V_{\rho}/V_{\sigma}$ and Emax were lower than those of the intact leaves. 4. The photosynthesis rate of tree leaves decreased remarkably with increasing the salt concentrations in soils, and it decreased faster for species intolerant cintolerant to salt.

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Studies on Salt Injuries of Seedlings Growth-Sand Media Effect of Various Concentration of Added Salts upon 3 Kinds of Seedlings (수묘(樹苗)의 염해(鹽害)에 관(關)한 연구(硏究) -Nacl 농도(濃度)를 달리한 사경(砂耕)에서의 몇 수묘(樹苗)에 미치는 영향(影響))

  • Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.8 no.1
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    • pp.3-10
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    • 1968
  • (1) The present work is a study on the tolerance of salt concentration, salt injuries and growth, under varied conditions, of salt concentration of five kind silvicultural species. (2) Pinus thunbergii, which is tolerant of stat, has shown both low fresh leaf's pH and low gravity, while Pinus densiflora, Abies bolophylla and Pinus koraiensis have respectively shown high fresh leaf's pH and high gravity. (3) Pinus thunbergii, which is tolerant otosalt, has shown high water content ind its lead, hbile such speciesras Pinus densiflora an Abies lophylla, which are intolerant of salt have shownlow water content in their leaves. (4) A signifficance has ibeen found, in the nonreatment fresh les's pH and n gravity, among Pinusthunbergiis Pinu rigida, Pinus koraiensis, Pinus densiflora and Abies holophya. (5) The order of thetlolerance of salt among the tested species is as foohws : Pinus thunbergii, P. Korea iensis, Abies holopylla. (6) The signs of salt injuries have been observed at the tops of the trees 2~3 hours after the application of 1.5% salt solution in the case of Abiihs holop hylla which are intolerant of salt, while they have been of served at the same position 4~6 hours after the 1.5% application in the case of Pinus Koraiensis and Pinus thunbergii.

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