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http://dx.doi.org/10.3839/jabc.2017.021

Isolation of salt-tolerant bacteria from rhizosphere and rhizoplane of halophyte plant Suaeda japonica in Gochang·Buan tidal flat  

Ki, Min-Gyu (Department of Bioenvironmental Chemistry, Chonbuk National University)
Lee, Hyeri (Department of Bioenvironmental Chemistry, Chonbuk National University)
Cho, Ahyeon (Department of Bioenvironmental Chemistry, Chonbuk National University)
Unno, Tatsuya (Faculty of Biotechnology, Jeju National University)
Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Chonbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.60, no.2, 2017 , pp. 125-131 More about this Journal
Abstract
Nine strains of high concentrations of salt-tolerant bacteria were isolated from the rhizosphere and rhizoplane of the halophyte plant Suaeda japonica grown in Gochang Buan tidal flat. The isolated bacteria were classified as genera Vibrio (strains JRS-1, -2, -3, -4, and -5, and JRL-1 and -4) and Bacillus (strains JRL-2 and -3) based on the 16S rRNA gene sequence similarity. The optical growth condition for salt concentration was examined on the selected, representative strains. Strain JRS-1 with the closest relative of Vibrio neocaledonicus showed the highest growth rate at the total salt concentration of 6% among the incubation conditions of 3-8% salt concentrations. Strain JRL-2 with the closest relative of Bacillus thuringiensis showed the tendency that growth rate increased with increasing salt concentrations and the maximum growth rate at 7% of the total salt concentration. The isolated bacteria showed salt-resistances to higher salt concentrations than their habitat soils with 3%. In addition, we identified evidences of potentially plant interaction-relevant enzymatic activities, from utilization of some substrates rich in plants, such as triglyceride, ${\rho}$-nitrophenyl-${\alpha}$,$\text\tiny{D}$-glucoside, and ${\rho}$-nitrophenyl-${\beta}$,$\text\tiny{D}$-glucoside.
Keywords
Halophyte; Rhizobacteria; Rhizoplane; Salt tolerance; Suaeda japonica;
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