• Title/Summary/Keyword: Salmonella Enteritidis

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Identification of Salmonella pullorum Genomic Sequences Using Suppression Subtractive Hybridization

  • Li, Qiuchun;Xu, Yaohui;Jiao, Xinan
    • Journal of Microbiology and Biotechnology
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    • v.19 no.9
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    • pp.898-903
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    • 2009
  • Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respectively, resulting in the identification of 20 subtracted fragments. A sequence homology analysis then revealed three types of fragment: phage sequences, plasmid sequences, and sequences with an unknown function. As a result, several important virulence-related genes encoding the IpaJ protein, colicin Y, tailspike protein, excisionase, and Rhs protein were identified that may play a role in the pathogenesis of S. Pullorum.

Biochemical characteristics and serotypes of Salmonella spp isolated from domestic animal in western Gyeongbuk province (경북서부지방 가축에서 분리된 Salmonella 속균의 생물화학적 특성 및 혈청형)

  • 김규태;김정화;우정희;장영술;김대원;김봉환
    • Korean Journal of Veterinary Service
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    • v.25 no.3
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    • pp.259-273
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    • 2002
  • The present study was conducted to investigate the prevalence, biochemical properties and serotypes of Salmonella organisms in the domestic animals in Western Gyeongbuk province during the period from January to December 2000. Salmonella spp were isolated from 51(1.62%) of 3,141 cases of domestic animals. Serotypes of isolates were S enteritidis 17(29.6% of isolates), S agona 11(20.3%), S rissen 8(14.8%), S gallinarum 4(7.4%), S derby 3(5.5%), S typhimurium 2(3.7%), S travis 1(1.8%), S montevideo 1(1.8%) and untypable 4(12.9%), in order. The majority of isolates were highly susceptible to amikacin, ampicillin, ciprofloxacin, cephalothin, chloramphenicol, trimethoprim-sulfamethoxazole, norfloxacin and gentamicin, whereas all isolates were resistant to bacitracin, erythromycin, tiamulin and tylosin, and the majority of them were highly resistant to penicillin, streptomycin and tetracycline.

A Report on the Salmonella Cultures Isolated in Korea(1973) (1973년(年) 한국(韓國)에서 분리(分離)된 쌀모넬라균(菌)에 관(關)한 보고(報告))

  • Ryu, Young-Hat;Kim, Young-Ja;Suh, Young-Sook
    • The Journal of the Korean Society for Microbiology
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    • v.9 no.1
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    • pp.1-5
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    • 1974
  • The authors identified 426 cultures of the Genus Salmonella among 1644 suspectable cultures of enteric pathogens submitted, to be bacteriologically confirmed, by the eleven hygiene laboratories of cities and provincial level and some hospital laboratories in 1973. According to the results obtained from the physical and biochemical tests, and the antigenic structural analyses, eleven cultures of Salmonella paratyphi B, nineteen cultures of Salmonella typhimurium, one each culture of Salmonella potsdam and Salmonella newport, sixteen cultures of Salmonella enteritidis and three hundred thirty-four cultures of Salmonella typhi were confirmed. The drugs of choice for treating Salmonella infections were found to be chloramphenicol, ampicillin and gentamycin in Korea, although there were a few cultures appearing in a couple of previous years which showed resistant patterns by the In Vitro tests.

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국내산 무화과의 식중독균에 대한 항균활성

  • 정미란;차정단;이영은
    • Proceedings of the Korean Society of Food and Cookery Science Conference
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    • 2003.10a
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    • pp.96-96
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    • 2003
  • 무화과의 성숙정도에 따른 항균활성을 검색하고자 메탄올과 용매계통분획물에 대하여 8종의 식중독균(Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella enteritidis, Vibrio parahaemolyticus, Salmonella typhimurium, Escherichia coli)에 대한 성장억제 효과를 검색하였다. 미숙, I, II기 및 성숙 무화과의 메탄올추출물은 10mg/mLshd도에서 L. monocytogenes, V. parahaemolyticus, E. coli O157:H7과 E. coli에 대해 강한 저해활성을 나타내었다. (중략)

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육계농장에서의 살모넬라 위생대책

  • 윤병선
    • KOREAN POULTRY JOURNAL
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    • v.34 no.5 s.391
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    • pp.79-82
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    • 2002
  • 육계농장에서는 SE(Salmonella Enteritidis)감염도 있지만, 오히려 SE이외의 여러 가지 요인에 의한 혈청형 살모넬라에 오염되는 경우가 많다. 현재 육계농장에서는 계군을 살모넬라로부터 완전히 차단하는 것은 어렵다고 생각하고 있고, WHO에서도 살모넬라 오염을 가능한 한 감소시키는 쪽으로 살모넬라 대책의 지침으로 정하고 있다. 그러나, SE 및 ST(Salmonella Typhimurium)등 사람에게 병원성을 나타내는 혈청형에 의한 계군의 살모넬라 감염오염은 공중위생의 견지에서 완전히 제거하지 않으면 안되다. 여기에서 출하 및 수송시의 대책, 사료의 살모넬라 오염의 중요성 등 산란계와는 다른 점을 지적하면서 기하고자 한다. 일상의 위생관리에 관한 양계장에서의 대책과 살모넬라 검사법에 대하여는 제외하였다.

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Bioserotype and drug resistance of Salmonella spp isolated from feces in zoo animals (동물원(動物園)의 야생동물(野生動物) 분변(糞便)에서 분리(分離)한 살모넬라균의 생물형(生物型), 혈청형(血淸型) 및 약제내성(藥劑耐性))

  • Youn, En-sun;Park, Seog-gee;Oh, Young-hee;Kim, Tae-jong
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.267-273
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    • 1994
  • Feces samples, obtained from zoo animals around Seoul, were examined for the isolation of Salmonella species, bioserotype and drug resistance for the prevention and treatment of salmonellosis, Salmonella spp were isolated 19(4.7%) from 408 samples of zoo animals. The subspecies in 19 Salmonella were all subspecies 1. The serological identification of Salmonella isolated were 31.6% in Sal typhimurium, 26.3% in Sal hadar, 21.1% in Sal muenchen, 15.8% in Sal enteritidis and 5.3% in Sal ayinde. The antibiotic resistance of Salmonella isolated were 13(68.4%) strains. The multiple resistant patterns of antibiotics in Salmonella were 2 drugs- and 3 drugs-resistance 30.8% respectively. The transferred rate of resistance to recipients(E coli ML 1410 $NA^r$) in Salmonella was 38.5%.

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Evaluation on Efficacies of Sodium Hypochlorite and Benzalkonium Chloride against Listeria monocytogenes and Salmonella spp. (Listeria monocytogenes와 Salmonella spp.에 대한 Sodium Hypochlorite 및 Benzalkonium Chloride의 유효성 평가)

  • Kim, Hyung-Il;Yoon, Hae-Jung;Choi, Hyun-Cheol;Jeon, Dae-Hoon;Eom, Mi-Ok;Sung, Jun-Hyun;Park, Na-Young;Won, Sun-Ah;Kim, Nam-Hee;Sung, Deok-Wha;Kwak, Hyo-Sun;Kwon, Ki-Sung;Lee, Young-Ja
    • Journal of Food Hygiene and Safety
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    • v.22 no.2
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    • pp.132-136
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    • 2007
  • Disinfectants/sanitizers, showing the bactericidal activity of $5log_{10}$ reduction against E. coli ATCC 10536 and Staphylococcus aureus ATCC 6538 used far assessing disinfectants/sanitizers efficacies, were examined whether showing similar efficacies against Salmonella spp. and Listeria monocytogenes, the major food poisoning bacteria, isolated from foods in current Korean market. The bactericidal efficacies on sodium hypochlorite and benzalkonium chloride were assessed by quantitative suspension tests in both 'clean' and 'dirty' conditions, respectively. Test organisms were consisted of E. coli ATCC 10536, Staphylococcus aureus ATCC 6538, Salmonella typhimurium ATCC 13311, Salmonella Enteritidis ATCC 13076, Listeria monocytogenes ATCC 19111, 10 kinds of Salmonella spp. from foods, and 11 kinds of Listeria monocytogenes from foods. More than $5log_{10}$ reduction in viable count for all strains was only achieved with benzalkonium chloride at the tolerance exemption concentration under dirty conditions. However, all strains were achieved more than $5log_{10}$ reduction under clean conditions.

A Case of Empyema and Mediastinitis by Non-typhi Salmonella (비장티푸스성 살모넬라 감염으로 발생한 농흉과 종격동염 1예)

  • Yang, Suh Yoon;Kwak, Hee Won;Song, Ju Han;Jeon, Eun Ju;Choi, Jae Cheol;Shin, Jong Wook;Kim, Jae Yeol;Park, In Won;Choi, Byoung Whui
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.6
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    • pp.537-540
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    • 2008
  • There are few reports of the pleuropulmonary involvement of a non-typhi Salmonella infection in immunocompromised patients with AIDS, malignancy, collagen vascular diseases, extended use of corticosteroids, sickle cell disease, or diabetes. We report a case of a non-immunocompromised patient who presented with concomitant empyema and mediastinitis due to Salmonella without a comorbid disease. A 26-year-old male patient, with a history of pneumonia 5 years earlier and having lived abroad for several years, presented chronic cough and febrile sensation. Pneumonia, empyema and mediastinitis were noted in a chest CT scan and Salmonella enteritidis and ${\beta}-hemolytic$ streptococcus were identified from a culture of the pleural fluid. Initially, he was treated with cefepime, metronidazole and clarithromycin. He was cured clinically and radiographically after an 8 week treatment with antibiotics. In conclusion, this report suggests that S. enteritidis can cause empyema and mediastinitis, albeit rarely.

Antagonistic Action of Lactobacilli Toward Pathogenic Bacteria in Associative Cultures (Lactobacillus spp.에 의한 병원성 세균의 생육저해)

  • 강국희;성문희
    • Journal of Food Hygiene and Safety
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    • v.4 no.2
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    • pp.155-163
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    • 1989
  • Three species of lactobacilli (L. casei, L. acidophilus, L. bulgaricus) were tested for their antibacterial activity. They all were antagonistic to growth of enteropathogenic Escherichia coli and Salmonella enteritidis in associative cultures in YS-medium (0.1 % yeast extract + skimmilk). Sal. enteritidis was more sensitive to the inhibition than was E. coli. Control cultures of E. coli and Sal. enteritidis were pH 5.08 and 5.70 in 72 hrs of incubation and the associative cultures were pH 3.35-4.48. The increases in pH resulting from growth of the lactobacilli in the associative cultures appeared to be sufficient and mainly responsible for the antagonistic actions exerted on the pathogens.

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The Antibacterial Action of Garlic, Onion, Ginger and Red Pepper Juice (마늘, 양파, 생강, 고추즙의 항균작용)

  • 서화중
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.1
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    • pp.94-99
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    • 1999
  • This study was carried out to determine the inhibitory effect of juice of garlic, onion, ginger and red pepper against Staphylococcus aureus, Salmonella enteritidis, Vibrio parahaemolyticus and Enterobacter cloacae which are food born disease organisms. It was found that 1~2.5%(wt/vol.) garlic juice showed strong antimicrobial action against growth of all test bacteria at 1/20$\times$10-6 dilution. Especially 1% garlic juice completly inactivated Vibrio parahaemolyticus but approximately 71.9~88% of reduction at same concentration in other bacteria was occurred. 2.5% onion juice had 28.5% antimicrobial activity on Sal. enteritidis, but 50% reduction was occurred on St.aureus and V. parahaemolyticus at same concentration. Antibacterial activity of ginger juice was similar to that of onion juice. 2.5% ginger juice showed approximately 50% reduction on Sal. enteritidis and V.parahaemolyticus, but less antimicrobial activity was occured on St. aureus and E. cloacae. Red pepper juice showed the least antimicrobial activity on food born disease organism compared to that of other juices. 2.5% red pepper juice showed 11.3%, 18.7% and 8.1% reduction on St. aureus, Sal. enteritidis and V.parahaemolyticus, respectively.

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