• Title/Summary/Keyword: Saline agricultural land

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Isolation and Identification of Alkali-tolerant Bacteria from Near-Shore Soils in Dokdo Island

  • Namirimu, Teddy;Kim, Jinnam;Zo, Young-Gun
    • Microbiology and Biotechnology Letters
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    • v.47 no.1
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    • pp.105-115
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    • 2019
  • Saline or alkaline condition in soil inhibits growth of most crop plants and limits crop yields in many parts of the world. Augmenting an alkaline soil with alkali-tolerant bacteria capable of promoting plant growth can be a promising approach in expanding fertile agricultural land. Near-shore environments of Dokdo Island, a remote island located in the middle of the East Sea, appear to have patches of seawater-influenced haloalkaline soil that is unsupportive for growth of conventional plants. To exploit metabolic capacities of alkali-tolerant bacteria for promoting plant growth in saline or alkaline soils, we isolated of alkali-tolerant bacteria from near-shore soil samples in Dokdo and investigated properties of the isolates. Alkali-tolerant bacteria were selectively cultivated by inoculating suspended and diluted soil samples on a plate medium adjusted to pH 10. Fifty colonies were identified based on their $GTG_5$-PCR genomic fingerprints and 16S rRNA gene sequences. Most isolates were affiliated to alkali-tolerant and/or halotolerant genera or species of the phyla Firmicutes (68%), Proteobacteria (30%) and Actinobacteria (2%). Unlike the typical soil bacterial flora in the island, alkali-tolerant isolates belonged to only certain taxa of terrestrial origin under the three phyla, which have traits of plant growth promoting activities including detoxification, phytohormone production, disease/pest control, nitrogen-fixation, phosphate solubilization or siderophore production. However, Firmicutes of marine origin generally dominated the alkali-tolerant community. Results of this study suggest that haloalkaline environments like Dokdo shore soils are important sources for plant growth promoting bacteria that can be employed in bio-augmentation of vegetation-poor alkaline soils.

Potential Productivity and Soil Morphological Characteristics of Paddy Land (답토양(沓土壤)의 형태적(形態的) 특성(特性)과 잠재생산력(潛在生産力)과의 관계(關係))

  • Moon, Joon;Um, Ki-Tae;Youn, Kwan-Hee
    • Korean Journal of Soil Science and Fertilizer
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    • v.14 no.4
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    • pp.174-178
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    • 1982
  • These studies were conducted to investigate the relation among soil morphological characteristics, soil group for management, suitability class and rice yield on nonfertilizer for four years (1976 to 1979) in paddy soils. The obtained results were summarized as follow ; 1. The best soil characteristics were that drainage was imperfectly drained, taxture family was fine loamy, and available soil depth was more than 100cm. 2. The mean of relative unhulled rice yield per 10a for highly productive, non-paddification, sandy, poorly drained and saline soil were 525, 492, 484, 474 and 263kg, respectively. 3. Those for 1st, 2nd, 3rd and 4th suitability class were 532, 510, 464 and 349kg, respectively.

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The Change of Physico-Chemical Properties of Paddy Soil in Reclaimed Tidal Land (간척지 논토양의 물리화학성 변동에 관한 연구)

  • Yang, Chang-Hyu;Yoo, Chul-Hyun;Jung, Ji-Ho;Kim, Byeong-Su;Park, Woo-Kyun;Ryu, Jin-Hee;Kim, Taek-kyum;Kim, Jae-Duk;Kim, Seong-Jo;Baek, Seung-Hwa
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.2
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    • pp.94-102
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    • 2008
  • The physico-chemical properties of ten reclaimed saline soils in five soil series of west-south Korea were analyzed according to the years past after reclamation. The soil samples were collected at the same sites two times in 2000 and 2004. The physico-chemical properties in 2000 had been changed in 2004 as follows. Soil salinity was the highest in Podu and desalinization period was the shortest in Munpo and Yeompo. Seasonal ground water level were above 100 cm in all regions that were 30 years old reclaimed tidal land, which was the same results of normal paddy field. In the case of soil physical changes, bulk density increased in fine textured soil (Poseung and Podu) but decreased in coarse textured soil (Gwanghwal, Munpo, and Yeompo). Porosity decreased in fine textured soil(Poseung and Podu) but increased in coarse textured soil. These reason were as follows. Fine textured soil were increased in solid phase but decreased in liquid and gaseous phase. Coarse textured soil, Gwanghwal and Munpo except for Yempo, were increased in gaseous phase but decreased in solid and liquid phase. Yempo that have low water table level were increased in liquid phase but decreased in solid and gaseous phase. Soil hardness increased in 4 soil series except for Munpo. In the case of chemical property changes, although there were more or less difference, it showed decreasing tendencies. Soil pH, the content of organic matter, available phosphate, and available silicate of five soil series were decreased during the four years. The content of exchangeable cation also decreased except for magnesium.

Changes of Soil Salinity due to Flooding in Newly Reclaimed Saline Soil (신간척지 토양에서 담수에 의한 토양염도 변화에 대한 개관)

  • Ryu, J.H.;Yang, C.H.;Kim, T.K.;Lee, S.B.;Kim, S.;Baek, N.H.;Choi, W.Y.;Kim, S.J.;Chung, D.Y.
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.spc
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    • pp.45-46
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    • 2009
  • This study was carried out to identify the changes of EC during desalinization due to flooding in newly reclaimed saline soil. To do this, experimental plots were made of rotary tillage+water exchanging plot, flooding plot and rainfall flooding plot. In rotary tillage+water exchanging plot, drainage, rotary tillage and flooding were conducted at the interval of 7 days. In rotary tillage+water exchanging plot and flooding plot, plots were irrigated at the height of 10 cm. After 38 days desalinization, changes of EC values at top soil (0~20 cm) were as follows. In rotary tillage+water exchanging plot, EC decreased from $21.38dS\;m^{-1}$ to $2.16dS\;m^{-1}$ and in flooding plot, EC decreased from $13.97dS\;m^{-1}$ to $2.22dS\;m^{-1}$. In rotary tillage+water exchanging plot and flooding plot, EC values decreased below the EC criterion ($4.0dS\;m^{-1}$) of saline soil. In rainfall flooding plot, EC values decreased or increased according to amounts of rainfall and rainfall time. After 38 days, EC decreased from $16.7dS\;m^{-1}$ to $12.35dS\;m^{-1}$. In flooding plot, changes of EC due to soil depth were investigated. After 38 days desalinization, changes of EC due to soil depth were as follows. At 0~10 cm depth, EC value decreased from $13.08dS\;m^{-1}$ to $0.74dS\;m^{-1}$ (94.3% of salt was desalinized). At 10~20 cm depth, EC value decreased from $14.80dS\;m^{-1}$ to $3.69dS\;m^{-1}$ (75.2% of salt was desalinized). At 20~30 cm depth, soil was desalinized slowly compared with upper soil, EC value decreased from $13.57dS\;m^{-1}$ to $6.93dS\;m^{-1}$ (48.9% of salt was desalinized).

Fresh-water Algae Occurred in Paddy Rice Fields III. Identification and Propagation of Green Algae (논발생(發生) 담수조류(淡水藻類)에 관(關)한 연구(硏究) III. 녹조류(綠藻類)의 동정(同定) 및 번식생태(繁殖生態))

  • Lee, H.K.;Park, J.E.;Ryu, G.H.;Lee, J.O.;Park, Y.S.
    • Korean Journal of Weed Science
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    • v.12 no.4
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    • pp.335-351
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    • 1992
  • The identification of green algae which were collected from paddy rice fields of the whole nation was conducted in 1991, and also the reproduction processes of three important green algae were investigated. The green algae identified were 46 species in 16 families including 4 species in the Volvocaceae, 4 species in the Palmellaceae, 3 species in the Ulotrichaceae, 3 species in the Oocystaceae and 6 species in the Scenedesmaceae. It was recognized that the algae which have caused a large injury to the flooded direct-seeded rice in Seosan reclaimed saline land was the genus Cladophora. The green water blooms on paddy water were induced mostly by the concentrated populations of suspended green algae such as the genera, Chlamydomonas and Stichococcus. A rapid propagation of Hydrodictyon was resulted from the asexual reproduction forming autocolony in each mother cell. The sexual and asexual reproduction processes of Oedogonium and the sexual reproduction process of Spirogyra were observed.

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Monitoring of Particulate Matter Concentration for Forage Crop Cultivation during Winter Season in Saemangeum (새만금 내 동계 사료작물 재배에 따른 미세먼지 농도 변화 모니터링)

  • Lee, Seong-Won;Kang, Bang-Hun;Seo, Il-Hwan
    • Journal of Bio-Environment Control
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    • v.31 no.2
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    • pp.114-124
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    • 2022
  • The Saemangeum has a dry surface characteristic with a low moisture content ratio due to the saline and silt soil, so the vegetation cover is low compared to other areas. In areas with low vegetation cover, wind erosion has a high probability of scattering dust. If the vegetation cover is increased by cultivating crops that can withstand the Saemangeum reclaimed environment, scattering dust can be reduced by reducing the flow rate at the bottom. Thus, the purpose of this study is to analyze the effect of suppressing the generation of fine dust and scattering dust by cultivating winter forage crops on the Saemangeum reclaimed land. While growing 0.5 ha of barley and 0.5 ha of triticale in Saemangeum reclaimed land, the concentration of fine dust was monitored according to agricultural work and growth stage. Changes in the concentrations of PM-10, PM-2.5, and PM-1.0 were monitored on the leeward, the windward and centering on the crop field. As a result of monitoring, PM-1.0 had little effect on crop cultivation. the concentration of PM-10 and PM-2.5 increased according to tillage and harvesting, and tillage had a higher increasing the concentration of PM-10 and PM-2.5 than that of harvesting. According to the growth stage of crops, the effect of suppressing scattering dust was shown, and the effect of suppressing scattering dust was higher in the heading stage than in the seedling stage. So, it was found that there was an effect of suppressing scattering dust other than the effect of land covering. Through this study, it was possible to know about the generation and suppression effect of scattering dust according to crop cultivation.

Corrosion Rate of Structural Pipes for Greenhouse (온실 구조용 파이프의 부식속도 검토)

  • Yun, Sung-Wook;Choi, Man Kwon;Lee, Si Young;Moon, Sung Dong;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.24 no.4
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    • pp.333-340
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    • 2015
  • Because soils in reclaimed lands nearby coastal areas have much higher salinity and moisture content than soils in inland area, parts of greenhouses embedded in such soils are exposed to highly corrosive environments. Owing to the accelerated corrosion of galvanized steel pipes for substrucrture and structure of greenhouses in saline environments, repair and reinforcement technologies and efficient maintenance and management for the construction materials in such facilities are required. In this study, we measured the corrosion rates of the parts used for greenhouse construction that are exposed to the saline environment to obtain a basic database for the establishment of maintenance and reinforcement standards for greenhouse construction in reclaimed lands with soils with high salinity. All the test pipes were exposed to soil and water environments with 0, 0.1, 0.3, and 0.5% salinity during the observation period of 480 days. At the end of the observation period, salinity-dependent differences of corrosion rate between black-surface corrosion and relatively regular corrosion were clearly manifested in a visual assessment. For the soils in rice paddies, the corrosion growth rate increased with salinity (0.008, 0.027, 0.036, and $0.043mm{\cdot}yr^{-1}$ at 0, 0.1, 0.3, and 0.5% salinity, respectively). The results for the soils in agricultural fields are 0.0002, 0.039, 0.040, and $0.039mm{\cdot}yr^{-1}$ at 0, 0.1, 0.3, and 0.5% salinity, respectively. The higher corrosion rate of rice-paddy soil was associated with the relatively high proportion of fine particles in it, reflecting the general tendency of soils with evenly distributed fine particles. Hence, it was concluded that thorough measures should be taken to counteract pipe corrosion, given that besides high salinity, the soils in reclaimed lands are expected to have a higher proportion of fine particles than those in inland rice paddies and agricultural fields.

Patterns of Leaching and Distribution of Cations in Reclaimed Soil according to Gypsum Incorporation Rate (석고 혼합량에 따른 간척지토양의 양이온 용탈 및 분포)

  • Ryu, Jin-Hee;Chung, Doug-Young;Hwang, Seon-Woong;Lee, Kyeng-Do;Lee, Sang-Bok;Choi, Weon-Young;Ha, Sang-Keun;Kim, Si-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.5
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    • pp.596-601
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    • 2010
  • Serious problems in reclaimed land for agriculture are high soil salinity and very poor vertical drainage. However, desalinization in these soils is very difficult. To identify the change of soil permeability by the gypsum incorporation in soils we observed elution patterns and salt distribution of the soil using soil columns packed with reclaimed saline soil with various rates of gypsum amendment. Saturated hydraulic conductivity ($K_{sat}$) of the top soil of reclaimed saline soils without gypsum incorporation was close 0 cm $hr^{-1}$ while $K_{sat}$ increased up to 0.3 cm $hr^{-1}$ with increasing amount of gypsum for 0.4% or more gypsum. Also $K_{sat}$ of the reclaimed saline soils for top soil was drastically increased to 1.0 cm $hr^{-1}$ or slightly greater after 141 hour's elution experiment. The cumulative amount of cation recovered in the effluent also increased in the order of gypsum incorporation rate 0.8% > 0.6% > 0.4 > 0.2%. Soil EC in soil columns decreased from initial 33.9 dS $m^{-1}$ to less than 0.4 dS $m^{-1}$ and exchangeable Ca 2+ increased by 32~140% according to gypsum incorporation rate.

Selection and Genetic Relationship of Salt Tolerant Rice Mutants by in vitro Mutagenesis

  • Song, Jae Young;Kim, Dong Sub;Lee, Myung-Chul;Lee, Kyung Jun;Kim, Jin-Baek;Kim, Sang Hoon;Yun, Song Joong;Kang, Si-Yong
    • Journal of Radiation Industry
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    • v.4 no.4
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    • pp.307-312
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    • 2010
  • Plants have evolved physiological, biochemical and metabolic mechanisms to increase their survival under the adverse conditions. This present study has been performed to select salt-tolerant rice mutant lines through in vivo and in vitro mutagenesis with gamma-rays. For the selection of the salt-tolerant rice mutants, we conducted three times of selection procedure using 1,500 gamma ray mutant lines resulted from an embryo culture of the original rice cv. Dongan (wild-type, WT): first, selection in the a nutrient solution with 171 mM NaCl; second, selection under in vitro condition with 171 mM NaCl; and third, selection in a reclaimed saline land. Based on a growth comparison of the entries, out of the mutant lines, two putative 2 salt tolerant (ST) rice mutant lines, ST-87 and ST-301, were finally selected. The survival rate of the WT, ST-87 and ST-301 were 36.6%, 60% and 66.3% after 7 days in 171 mM NaCl treatment, respectively. The WT and two salt tolerant mutant lines were used to analyze their genetic variations. A total of 21 EcoRI and Msel primer combinations were used to analyze the genetic relationship of among the two salt-tolerant lines and the WT using the ABI3130 capillary electrophoresis system. In the AFLP analysis, a total of 1469 bands were produced by the 21 primer combinations, and 700 (47.6%) of them were identified as having polymorphism. The genetic similarity coefficients were ranged from 0.52 between the ST-87 and WT to 0.24 between the ST-301 and the WT. These rice mutant lines will be used as a control plot for physiological analysis and genetic research on salt tolerance.

Yield Response of Chinese Cabbage to Compost, Gypsum, and Phosphate Treatments under the Saline-sodic Soil Conditions of Reclaimed Tidal Land (퇴비, 석고, 인산으로 개량한 염류-나트륨성 간척지 토양에서 배추의 생육)

  • Lee, Jeong-Eun;Seo, Dong-Hyuk;Ro, Hee-Myong;Yun, Seok-In
    • Horticultural Science & Technology
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    • v.34 no.4
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    • pp.587-595
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    • 2016
  • Salt stress in crops in reclaimed tidal lands can be reduced by applying soil amendments. To evaluate the effects of compost, gypsum, and phosphate on the growth of Chinese cabbage in saline-sodic soil conditions, we conducted a pot experiment in 2013 and 2014. The treatments consisted of a standard fertilizer application of a mix of compost and N-P-K fertilizer (S) and standard fertilizer applications with additional compost (S + C), gypsum (S + G), phosphate (S+P), and gypsum and phosphate (S + GP). The mean dry matter yield of cabbage in 2014 was three times as great as that in 2013, although soil EC (Electrical conductivity) in 2014 was not decreased. However, the mean ratio of sodium ion in soil solution ($SAR_{1:5}$) significantly decreased from $17.3{\pm}1.1$ in 2013 to $11.2{\pm}2.7$ in 2014. Application of gypsum had the greatest positive impact on the growth of Chinese cabbage. The S + G treatment increased dry matter yield by 7.0 (48.2) and 7.9 g/plant (16.6%) in 2013 and 2014, respectively, compared to the S treatment. Applying gypsum increased soil EC, but decreased $SAR_{1:5}$ by 14 and 38% in 2013 and 2014, respectively. The application of compost and phosphate had a small effect on the growth of Chinese cabbage. These results suggest that applying gypsum in reclaimed tidal lands can reduce the sodicity of the soil and improve crop growth.