• 한국항공운항학회지
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• 제20권4호
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• pp.91-105
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• 2012

This study, the cause of an aviation security employees' job satisfaction and dissatisfaction will be understood and the data can be used as evidence to explain the position of the employees. Moreover, the results of this study shall be conducted to increase the level of service delivery and also to remove unsatisfactory factors. Thus, according to this research survey shows the understanding of the job satisfaction and service delivery influences on the overall job satisfaction of a secure enterprise's security screeners. As a results, by improving the job satisfaction of aviation security screeners, the world's most safe, convenient service and quick airport service delivery could be achieved. Therefore, in order to improve the level of Korea and the international competitiveness of airlines, the promotion of job satisfaction of employees would be very important. Moreover based on the results of this study, the advanced research on the recognition differences for service evaluation between the customers and security screeners are needed.

• Journal of Pharmaceutical Investigation
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• 제30권1호
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• pp.1-12
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• 2000

Gene therapy is a method for the treatment of diseases with introducing the gene-engineered materials into a patient with gene-deficiency disease (e.g. cystic fibrosis) or cancer to produce a therapeutic protein in a patient's cells. Successful gene therapy requires establishing both gene expression systems and delivery systems. Viral and non-viral vectors have been used for gene delivery. Viral vectors have a high transfection efficiency, but are limited in relations to issues of safety, toxicity and immunogenecity. Non-viral vectors are easy to prepare and relatively safe. However, non-viral vectors have a low transfection efficiency. Cationic liposomes are the most available among non-viral vectors. Cationic liposomes have been used to transfect cells both in vitro and in vivo experiments. Besides, several formulations containing cationic lipid are being used in clinical trials in cases of cystic fibrosis or cancer. A crucial subject to the further development of gene delivery vectors will be a long-term gene expression with following characteristics; protecting and deliverying DNA efficiently, non-toxic and non-immunogenic, and easy to produce in large scale.

• 한국어병학회지
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• 제34권2호
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• pp.177-184
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• 2021

Vaccines based on single-cycle viruses that are replication-incompetent due to knockout of replication-related structural gene(s) are more immunogenic than inactivated or subunit vaccines and can be used as delivery vehicles for foreign antigens without concerns on the reverting to virulent forms. The aim of this study was to develop a delivery vehicle for nervous necrosis virus (NNV)-like particles (VLPs) using G gene deleted single-cycle VHSV (rVHSV-𝚫G). Recombinant single-cycle VHSVs carrying NNV capsid protein gene between N and P gene of rVHSV-𝚫G genome (rVHSV-𝚫G-NNV_Cap) were rescued by reverse genetic technology. The successful expression of NNV capsid protein in cells infected with rVHSV-𝚫G-NNV_Cap was demonstrated by Western blot analysis, and the production of NNV VLPs in infected cells was confirmed using an electron microscopy. The results suggest that single-cycle VHSVs can be used as a safe delivery vehicle for NNV VLPs, and can be extended to other pathogens for the development of prophylactic vaccines.

• BMB Reports
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• 제52권5호
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• pp.324-329
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• 2019

Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols.

• International Journal of Internet, Broadcasting and Communication
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• 제13권4호
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• pp.90-96
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• 2021

The logistics market is continuously growing due to the development of technology and the growth of the online market. In addition, the social atmosphere that emphasizes non-face-to-face due to the pandemic situation is accelerating the growth of logistics. Delivery of goods ordered online requires delivery process through courier worker. In order for the courier worker to ship the product, the work of loading the product on the truck must be preceded. The accident caused by such delivery and loading work is increasing and it is emerging as a social problem. This study proposes a robot-based automated loading system to efficiently handle the increasing volume of courier service and to construct a more efficient and safe working environment by replacing the physical labor that was overloaded to courier workers. The proposed system replaces the loading of the courier worker and proposes the optimal loading function through the automation system.

• ETRI Journal
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• 제45권2호
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• pp.329-337
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• 2023

For safe last-mile autonomous robot delivery services in complex environments, rapid and accurate collision prediction and detection is vital. This study proposes a suitable neural network model that relies on multiple navigation sensors. A light detection and ranging technique is used to measure the relative distances to potential collision obstacles along the robot's path of motion, and an accelerometer is used to detect impacts. The proposed method tightly couples relative distance and acceleration time-series data in a complementary fashion to minimize errors. A long short-term memory, fully connected layer, and SoftMax function are integrated to train and classify the rapidly changing collision countermeasure state during robot motion. Simulation results show that the proposed method effectively performs collision prediction and detection for various obstacles.

• 대한안전경영과학회지
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• 제25권2호
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• pp.91-97
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• 2023

Ensuring the safe arrival of delivery cargo at its intended destination is of utmost importance. Truck drivers play a crucial role in guaranteeing the secure delivery of cargo without any mishaps. However, there are various factors that may lead to delayed arrival of trucks at their destination, such as late departures or prolonged loading operations. The timely departure of cargo transportation is contingent upon several variables, including the driver's experience, cargo volume, and loading time. If the transportation commencement is delayed, it may increase the risk of accidents due to an elevated operating speed. Consequently, we conducted a study to investigate the correlation between cargo loading time, cargo volume, driving experience, and the likelihood of accidents. Our findings indicate that both cargo volume and driver experience can impact the likelihood of vehicle accidents. Furthermore, all factors can have an interactive effect on the occurrence of accidents. However, extending the loading time may mitigate the impact on the likelihood of accidents.

• 대한영양사협회학술지
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• 제28권3호
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• pp.182-194
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• 2022

After the onset of the COVID-19 pandemic, there has been an explosive increase in restaurant meal delivery or takeout. The purpose of this study was to analyze the consumer perception of food safety and its influence on the purchase of delivery or takeout food from restaurants. This study, the 2020 Consumer Behavior Survey for Food (CBSF), was conducted from June 10 to August 21 2020. A total of 6,355 responses were used for the analysis. The results were as follows: First, the differences in consumer perception about food safety were analyzed according to whether they used delivery or takeout. Concern about food safety, satisfaction with dietary habits, and the ability to maintain safe dietary habits were higher in the non-user group. Except for food at home, the perception about food safety at other locations was higher in the user group. Food hazards such as antibiotics were perceived to be safer in the user group. Second, the perception of food safety affecting use of delivery or takeout was analyzed. It was found that the usage of delivery or takeout increased when the perception of the safety of home meal replacement (HMR), delivery or takeout food, and the ability to be informed about the harmful factors of agricultural products increased. The findings of this study may offer the basis for the food and food service industry to consider safety issues seriously and develop strategies to lead to feasible practices. Further, this study also supports the direction of the government toward strengthening the safety of new segments which have shown explosive growth in the COVID-19 era.

• Bulletin of the Korean Chemical Society
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• 제25권1호
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• pp.122-126
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• 2004

Biofunctional nanohybrids are synthesized from layered double hydroxide (LDH) and the vitamins such as ascorbic acid and topopherol acid succinate. Either ion exchange or copricipitaion leads to successful intercalation of the vitamins into gallery space of LDH that offers a new route to safe preservation of bioactivity as well as controlled release. Intercalations of vitamins are clearly reflected on the increase in the basal spacing of ZnAl-(Nitrate) LDH from 8.5 ${\AA}$ to 10.5 ${AA}$ for ascorbate, and 49.0 ${AA}$ for tocopherol acid succinate, respectively. No significant change in UV-Vis and IR absorption characteristics of the intercalated vitamins strongly supports the safe maintenance of their bioactivities without any deterioration of chemical and structural integrity. Furthermore, it is shown that the hybridized vitamins could be discharged in a controlled kinetics.

• Journal of Plant Biotechnology
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• 제37권3호
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• pp.250-261
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• 2010

The expression of vaccine antigens in transgenic plants has the potential to provide a convenient, stable, safe approach for oral vaccination alternative to traditional parenteral vaccines. Over the past two decades, many different vaccine antigens expressed via the plant nuclear genome have elicited appropriate immunoglobulin responses and have conferred protection upon oral delivery. Up to date, efforts to produce antigen proteins in plants have focused on potato, tobacco, tomato, banana, and seed (maize, rice, soybean, etc). The choice of promoters affects transgene transcription, resulting in changes not only in concentration, but also in the stage tissue and cell specificity of its expression. Inclusion of mucosal adjuvants during immunization with the vaccine antigen has been an important step towards the success of plant-derived vaccines. In animal and Phase I clinical trials several plant-derived vaccine antigens have been found to be safe and induce sufficiently high immune response. Future areas of research should further characterize the induction of the mucosal immune response and appropriate dosage for delivery system of animal and human vaccines. This article reviews the current status of development in the area of the use of plant for the development of oral vaccines.