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Construction of Bifunctional Fusion Enzyme between Maltooligosyltrehalose Synthase and Maltooligosyltrehalose Trehalohydrolase of Sulfolobus acidocaldarius and Overexpression in E. coli

  • Kim, Chung Ho
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.240-245
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    • 2000
  • Two genes encoding maltooligosyltrehalose synthase (SaMTS) and maltooligosyltrehalose trehalohydrolase (SaMTH) were isolated from a hyperthermophilic microorganism, Sulfolobus acidocaldarius (ATCC 49462). ORFs of the SaMTS and SaMTH genes are 2,163 and 1,671 bp long and encode 720 and 556 amino acid residues, respectively. A bifunctional fusion enzyme (SaMTSH) was constructed through the gene fusion of SaMTS and SaMTH. Recombinant SaMTS, SaMTH, and SaMTSH fusion enzyme were overexpressed in E. coli BL21. SaMTS and SaMTH produced trehalose and maltotriose from maltopentaose in a sequential reaction. SaMTSH fusion enzyme catalyzed the sequential reaction in which the formation of maltotriosyltrehalose was followed by hydrolysis leading to the synthesis of trehalose and maltotriose. The SaMTSH fusion enzyme showed the highest activity at pH 5.0-5.5 and $70-75^{\circ}C$. SaMTS, SaMTH, and SaMTSH fusion enzyme were active in soluble starch, which resulted in the production of trehalose.

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The Effect of Salicylic Acid (SA) on the Photosynthetic Activity in Commelina communis L. (Salicylic acid가 닭의장풀의 광합성에 미치는 영향)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.17 no.3
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    • pp.359-364
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    • 1999
  • The effect of Salicylic acid (SA) on the leaf growth, chlorophyll content, photosynthesis, stomatal conductance in Commelina communis L. was investigated. C. communis which was grown in Hoagland solution containing 1 mM SA during 3 weeks resulted in a significant reduction of leaf length, width and area. 1 mM SA reduced about 10% of the leaf area at 1 week and 2 weeks, but inhibited 22% of the leaf area at 3 weeks. SA also showed great effect on the reduction of chlorophyll content. SA reduced 47% and 53% of chlorophyll content each at 2 weeks and 3 weeks when it was compared with the control. Chlorophyll a/b ratio in SA treated sample was increased at 3 weeks representing that SA was sensitive to chlorophyll b. The treatment of SA did not inhibit the activity of PSII+PSI, PSII and PSI. This result indicates that SA did not show direct effect oil the photosynthetic electron transport activity. However, when C. communis was grown in Hoagland solution containing I mM SA fer long period, SA showed clear inhibition of photosyhthesis in intact leaves. The treatment of SA for 3 weeks showed about 23~65% inhibition of photosynthetic activity at various light intensity (100~1000$\mu$mo1 m­$^2$$^1$). Similar effect was found on stomatal conductance. The treatment of SA caused an almost closure of stomata. Similar effects of stomatal conductance at various light intensity indicate a loss of normal control on stomatal mechanism. These results indicate that the effect of SA on photo-synthetic activity was not by SA itself but by indirect metabolic pathway.

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잇사매와 함사매의 名稱

  • 김진구
    • The Research Journal of the Costume Culture
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    • v.7 no.2
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    • pp.220-224
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    • 1999
  • This study is concerned with the names of is sa mai(잇사매) and ham sa mai(함사매). Origins and meanings of these terms were traced and examined. Some significant findings can be summarized as follows : Korean word is sa mai(잇사매) was a compound word. Korean character is (잇) is derived from Chinese character i, and sa mai(사매) in Korean means sleeves 神 in Chinese character. The character ham in Ham sa mai(함사매) is its origin in Chinese : Korean character ham was Korean pronounciation of the Chinese character, han. ham sa mai is a compound word of ham(함) (Korean), (han ) (Chinese) and sa mai(사매) sleeves(Korean). Thus, first characters of is(잇) in is sa mai(잇사매) and ham(함) in ham sa mai(함사매) are originated from Chinese, where as the word sa mai in the end of is sa mai and ham sa mai is pure Korean. Both character i and ham(han, Chinese) in Chinese means a jaw. Thus, both is sa mai(잇사매) and ham sa mai(함사매) means “jawed sleeves”. That is long and wide round sleeves with narraw wrists.

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The Reverse Effect of Salicylic Acid on Cd-induced Growth, Chlorophyll, and Rubisco/Rubisco Activase in Tobacco (카드뮴에 의해 유도된 담배 식물의 생장, 엽록소와 rubisco/rubisco activase에 대한 salicylic acid의 전환 효과)

  • Wang, Yu Shan;Roh, Kwang-Soo
    • Journal of Life Science
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    • v.22 no.6
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    • pp.778-787
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    • 2012
  • The influence of salicylic acid (SA) on growth, chlorophyll, and rubisco/rubisco activase and effect of denaturator on rubisco/rubisco activase activity were studied in tobacco plants grown in vitro with cadmium (Cd) treatment. In order to find out the optimum concentration of SA, tobacco plants treated with $10^{-6}$ mM - $10^2$ mM of SA were grown in MS medium for 9 weeks, respectively. The most pronounced effect on in vitro growth was found at $10^{-4}$ mM SA. Among the control (not treated with Cd and SA), SA, Cd, and Cd + SA, the growth and content of chlorophyll were in the sequence of Cd < Cd + SA < control < SA, and significantly higher at SA compared with others. Similar results were also observed in the content and activity of rubisco and rubisco activase. These data suggest that inhibitory effect by Cd was reversed by SA. These results also indicate that SA has a positive effect on Cd. The effect of denaturants on rubisco activity showed in the sequence of Cd < Cd + SA < control < SA. Rubisco activity was promoted by L-cysteine and ${\beta}$-mercaptoethanol, not by urea, thiourea, and guanidium-HCl. These data suggest that urea, thiourea, and guanidium-HCl are able to act as denaturator, and L-cysteine and ${\beta}$-mercaptoethanol are not. None of the five denaturants affected the activity of rubisco activase.

A Study on Sa(紗) and Ra(羅) at the End of the Joseon Period (조선 말기 사ㆍ라에 관한 연구)

  • 이은진;조효숙
    • Journal of the Korean Society of Costume
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    • v.53 no.8
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    • pp.121-135
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    • 2003
  • The purpose of this study is to suggest a theoretical basis to name Sa(紗) and Ra(羅) remains properly by comparing and analyzing the name, usage, value, length and width of Sa(紗) and Ra(羅) recorded on documents made at the end period of Joseon. The features of Sa(紗) and Ra(羅) at the end of the Joseon Period are as follows. 1. The kinds of Sa(紗) are about 80, those of Ra(羅) are about 12, and those of Sa(紗) are significantly more than those of Ra(羅). In regard to the aspect of patterns, there were about 20 types of patterns in the case of Sa(紗), but no specific pattern for Ra(羅). 2. Some newly revealed patterns in the case of Sa (紗) are as follows. Baek -bok-mun(백복문) was a pattern full of ‘bats(박쥐[복])’, and Baek-jeop-mun(백접문) was a pattern full of ‘butterflies(나비[蝶])’ Jeop-mun(접문) was classified into ‘butterfly patterns(나비문[접문])’ and ‘traditional window flame patterns(창살문[접문])‘. 3. When considering the usages of Sa(紗) and Ra(羅), Sa(紗) was used for various detailed purposes according to their kinds and patterns, but Ra(羅) was mostly used for underwear. The most commonly used Sa(紗) was the Gab-sa type(甲紗類). On the contrary, the Go-sa type(庫紗類) was significantly less used than the Gab-sa type(甲紗類). However, it must have been of relatively high quality Sa(紗), shown by the fact that it was used for outer garments. In addition, the Gung-sa type(宮紗類) was the best quality Sa(紗), shown by the fact that it was used for court dress and official uniforms in the royal court. 4. Sa(紗) and Ra(羅) whose features have been examined we Gapsa(甲紗), Sun-in (純仁), Gosa(庫紗), Gwansa(官紗), Jusa(走紗), Eunjosa(은조사), Gwangsa(廣紗), Waesa(倭紗), Dorisa(도리사), Gong-yangsa(공양사), Rasa(羅紗), Danghangra(唐亢羅), Yanghangra(洋亢羅), Yunjura(윤주라), Eunra(銀羅), Jeohangra(저항라), Chura(秋羅). 5. Regarding the values of Sa(紗) and Ra(羅), they were high quality textures and its length and width of 1 Pil(疋), a roll of cloth, were not subdivided in detail such as in the case of plain weaved silks(平絹).

The Expression Patterns of AtBSMT1 and AtSAGT1 Encoding a Salicylic Acid (SA) Methyltransferase and a SA Glucosyltransferase, Respectively, in Arabidopsis Plants with Altered Defense Responses

  • Song, Jong Tae;Koo, Yeon Jong;Park, Jong-Beum;Seo, Yean Joo;Cho, Yeon-Jeong;Seo, Hak Soo;Choi, Yang Do
    • Molecules and Cells
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    • v.28 no.2
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    • pp.105-109
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    • 2009
  • We reported previously that overexpression of a salicylic acid (SA) methyltransferase1 gene from rice (OsBSMT1) or a SA glucosyltransferase1 gene from Arabidopsis thaliana (AtSAGT1) leads to increased susceptibility to Pseudomonas syringae due to reduced SA levels. To further examine their roles in the defense responses, we assayed the transcript levels of AtBSMT1 or AtSAGT1 in plants with altered levels of SA and/or other defense components. These data showed that AtSAGT1 expression is regulated partially by SA, or nonexpressor of pathogenesis related protein1, whereas AtBSMT1 expression was induced in SA-deficient mutant plants. In addition, we produced the transgenic Arabidopsis plants with RNAi-mediated inhibition of AtSAGT1 and isolated a null mutant of AtBSMT1, and then analyzed their phenotypes. A T-DNA insertion mutation in the AtBSMT1 resulted in reduced methyl salicylate (MeSA) levels upon P. syringae infection. However, accumulation of SA and glucosyl SA was similar in both the atbsmt1 and wild-type plants, indicating the presence of another SA methyltransferase or an alternative pathway for MeSA production. The AtSAGT1-RNAi line exhibited no altered phenotypes upon pathogen infection, compared to wild-type plants, suggesting that (an)other SA glucosyltransferase(s) in Arabidopsis plants may be important for the pathogenesis of P. syringae.

A Predictive Model of Situation Awareness with ACT-R

  • Kim, Junghwan;Myung, Rohae
    • Journal of the Ergonomics Society of Korea
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    • v.35 no.4
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    • pp.225-235
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    • 2016
  • Objective: The aim of this study is to model all levels of situation awareness (SA), which would be able to predict situation awareness quantitatively. Background: When measuring situation awareness, directly measuring SA methods such as SAGAT and SART have been utilized. Several approaches (cognitive modeling approaches) were introduced to model SA but level 3 SA was not completed. For real-life situation, however, it is necessary to detect the problematic level of SA rather than overall SA. Therefore, we proposed a new model of all levels of SA in this study. Method: In order to model all levels of SA, this study chose factors in ACT-R architecture through literature review. ATC (Air Traffic Control)-related simulation task was video-taped to analyze human behaviors in order to model all levels of SA including level 3. Results: As a result, regression analyses show that cognitive activities (neural activations) represented for all levels of SA were highly correlated with SAGAT. Conclusion: In conclusion, neural activations in ACT-R could be proved to be effective to model all levels of SA. Application: Our SA model could be used to predict all levels of SA quantitatively without directly measuring the SA of operators.

SA-SUPPLEMENT SUBMODULES

  • Durgun, Yilmaz
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.1
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    • pp.147-161
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    • 2021
  • In this paper, we introduced and studied sa-supplement submodules. A submodule U of a module V is called an sa-supplement submodule in V if there exists a submodule T of V such that V = T + U and U ∩ T is semiartinian. The class of sa-supplement sequences ������ is a proper class which is generated by socle-free modules injectively. We studied modules that have an sa-supplement in every extension, modules whose all submodules are sa-supplement and modules whose all sa-supplement submodules are direct summand. We provided new characterizations of right semiartinian rings and right SSI rings.

Improved Simulated-Annealing Technique for Sequence-Pair based Floorplan (Sequence-Pair 기반의 플로어플랜을 위한 개선된 Simulated-Annealing 기법)

  • Sung, Young-Tae;Hur, Sung-Woo
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.4
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    • pp.28-36
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    • 2009
  • Sequence-Pair(SP) model represents the topological relation between modules. In general, SP model based floorplanners search solutions using Simulated-Annealing(SA) algorithm. Several SA based floorplanning techniques using SP model have been published. To improve the performance of those techniques they tried to improve the speed for evaluation function for SP model, to find better scheduling methods and perturb functions for SA. In this paper we propose a two phase SA based algorithm. In the first phase, white space between modules is reduced by applying compaction technique to the floorplan obtained by an SP. From the compacted floorplan, the corresponding SP is determined. Solution space has been searched by changing the SP in the SA framework. When solutions converge to some threshold value, the first phase of the SA based search stops. Then using the typical SA based algorithm, ie, without using the compaction technique, the second phase of our algorithm continues to find optimal solutions. Experimental results with MCNC benchmark circuits show that how the proposed technique affects to the procedure for SA based floorplainning algorithm and that the results obtained by our technique is better than those obtained by existing SA-based algorithms.

Biochemistry of Salicylic Acid and its Role in Disease Resistance

  • Lee, Hyung-Il;Raskin, Ilya
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.4
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    • pp.233-238
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    • 1997
  • Salicylic acid (SA) is involved in the establishment of systemic acquired resistance (SAR) in many plant including tobacco. Considering the important role of SA in disease resistance, biosynthetic and metabolic pathways of SA in tobacco have been studied extensively: The initial step for biosynthetic pathway of SA is conversion of phenylalanine to trans-cinnamic acid, followed by decarboxylation of trans-cinnamic acid to benzoic acid and ie subsequent ring hydroxylation at the C-2 position to form SA. In TMV inoculated tobacco, most of the newly synthesized SA is glucosylated or methylated. Methyl salicylate has been identified as a biologically active, volatile signal. In contrast, the two glucosylated forms accumulate in the vicinity of lesions and consist of SA glucoside, a major metabolite, and SA glucose ester, a relatively minor from. Two enzymes involved in SA biosynthesis and metabolism have been purified and characterized : benzoic acid 2-hydroxylase which catalyzes conversion of benzoic acid to SA; UDP-Glucose: SA 1-O-D glucosyltransferase which converts SA to SA glucose ester. Further studies of the biosynthetic and metabolic pathways of SA will help to elucidate the SAR signal transduction pathway and provide potential tools for the manipulation of disease resistance.

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