Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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The Effect of Salicylic Acid (SA) on the Photosynthetic Activity in Commelina communis L.

Salicylic acid가 닭의장풀의 광합성에 미치는 영향

  • 이준상 (상지대학교 생명과학과)
  • Published : 1999.09.01
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Abstract

The effect of Salicylic acid (SA) on the leaf growth, chlorophyll content, photosynthesis, stomatal conductance in Commelina communis L. was investigated. C. communis which was grown in Hoagland solution containing 1 mM SA during 3 weeks resulted in a significant reduction of leaf length, width and area. 1 mM SA reduced about 10% of the leaf area at 1 week and 2 weeks, but inhibited 22% of the leaf area at 3 weeks. SA also showed great effect on the reduction of chlorophyll content. SA reduced 47% and 53% of chlorophyll content each at 2 weeks and 3 weeks when it was compared with the control. Chlorophyll a/b ratio in SA treated sample was increased at 3 weeks representing that SA was sensitive to chlorophyll b. The treatment of SA did not inhibit the activity of PSII＋PSI, PSII and PSI. This result indicates that SA did not show direct effect oil the photosynthetic electron transport activity. However, when C. communis was grown in Hoagland solution containing I mM SA fer long period, SA showed clear inhibition of photosyhthesis in intact leaves. The treatment of SA for 3 weeks showed about 23~65% inhibition of photosynthetic activity at various light intensity (100~1000$\mu$mo1 m­$^2$S­$^1$). Similar effect was found on stomatal conductance. The treatment of SA caused an almost closure of stomata. Similar effects of stomatal conductance at various light intensity indicate a loss of normal control on stomatal mechanism. These results indicate that the effect of SA on photo-synthetic activity was not by SA itself but by indirect metabolic pathway.

살리실릭산(SA)이 닭의장풀의 잎의 생장, 엽록소 함량, 광합성능, 기공전도도에 미치는 영향을 조사하였다. Hoagland 용액에 SA를 처리한 후 3주 동안 배양한 닭의장풀은 잎의 길이, 너비, 면적이 크게 감소하였다. 1 mM SA를 1, 2주 처리 시에 엽면적 생장이 약 10%억제되었으며, 3주 째에는 22%억제하였다. SA는 또한 2주 째는 47%그리고 3주 째는 53%엽록소 함량을 감소시켰다. SA를 처리한 샘플의 엽록소 a/b는 3주 째에 약 3으로 크게 증가하였다. SA를 분리 엽록체에 직접 처리하여 전자전달활성을 직접 측정하였다. PSII＋PSI, PSII 그리고 PSI활성에 SA가 영향을 주지 않았다. 이는 SA가 직접 광합성 기작에는 영향을 주지 않는 다는 것을 나타낸다. 그러나, SA를 오랜 기간 처리하면 광합성의 감소는 매우 뚜렷하게 관찰되었다. Hoagland 용액에 SA를 처리한 후 3주 동안 배양한 닭의장풀에 다양한 빛 광도(100~1000$\mu$mo1 m­$^2$S­$^1$)를 조사하면 약 23~65%의 광합성능의 저하가 나타났다. 기공전도도가 유사한 반응을 보여주었다. SA처리구의 기공은 거의 닫혔다. 여러 광조건에서 기공전도도가 같다는 것은 SA가 기공의 조절 기능을 상실하게 한 것으로 보여진다. 이들 결과들로부터 광합성능에 대한 SA의 효과는 SA자체에 의한 것이 아니라 간접적인 대사 경로를 통해 매개되는 것으로 추측된다.

Keywords

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