• Food Science of Animal Resources
• /
• v.27 no.1
• /
• pp.95-101
• /
• 2007

We have investigated the immunomodulatory activity of water extracts of Acanthopanax divaricatus var. alveofructus in male ICR mice. Mice were administrated with fermented milk containing freeze-dried extract 3 mg/Kg (A), 9 mg/kg (B), 27 mg/Kg (C) per body weight with A. divaricatus var. alveofructus (loots : leaves : stem) : Codonopsis lanceolata = (5 : 2 : 1.5) : 1.5 for 7 and 10 weeks, respectively. Body weight, relative organ weight, cellularity of lymphoid organs, plaque- forming cell (PFC) assay, agglutination (AGG) test and lymphoproliferation were examined in various groups of animals. Any significant differences of body weight gain were not recorded in the tested ICR mice. There was significant different (p<0.05) in the spleen index in B group of 10 weeks and C group of 7 weeks fed mouse. The thymus gain weight was increased during administration of the extract, but there was no significant increase on other organs gain. Humoral immunity as measured by PFC showed more decreased PFC level in 10 weeks than in 7 weeks. In the HT, A. divaricatus var. albeofructus extract also showed a significant increase (p<0.05) in C group of 10 weeks. Administration of extracts from A. divaricatus var. albeofructus increased significantly in the production of IgG antibodies on the mice immunized with SRBC in B group of 7 and 10 weeks (p<0.05).

• Research in Plant Disease
• /
• v.14 no.3
• /
• pp.159-164
• /
• 2008

Crown gall of rose (Rosa hybrida) was observed in greenhouse during 2003-2007. The average disease incidence was up to 38.1 % and was more severe in hydroponic culture as compared to soil culture. The typical gall symptom occurred mainly on the root, crown, or both, and resulted on poor rooting, growth retardation and yield loss. The reduction rate of rooting influenced by crown gall was 57.5% as compared to healthy plants on nursery stock. The location of gall formation in the plant influenced growth vigor resulting in symptoms such as poor shooting. Healthy plants produced 19.1 flowers/$m^2$, while diseased plants produced 9.5 flowers/$m^2$ during the same cultivation period. Moreover, the number of days to flowering was longer for the diseased plants than for healthy plants - 51.2 days and 39.8 days for first harvest, and 60.6 days and 52.1 days for the second harvest, respectively. Conclusively, infection on the basal stem caused serious loss of the number of shoot formation; yield loss of cut flower was 38.7% due to crown gall infection and delay of harvesting time about 8-10 days.

• Korean Journal of Soil Science and Fertilizer
• /
• v.34 no.5
• /
• pp.358-364
• /
• 2001

To obtain information for the proper fertilization management of cut-flower roses, the amount of macro and micro elements absorbed by cut-flower roses from soil for a year was investigated. Three cut-flower rose cultivars which are called 'Grandgara', 'Nobless', and 'Centina' were transplanted to a sandy loam soil, N-P-K standard fertilization was applied to the soil, and drip irrigation was done at the level of 10 kPa soil moisture tension. There was not significantly different in the harvest amount of cut-flower rose between 'Grandgara' and 'Nobless', but the harvest yield of 'Centina' was about 63% level when compared to that of 'Grandgara'. Considering seasonal changes in the content of nutrients in plant, parts, the uptake of untrients was higher in winter season than that in spring and summer seasons. Except for 'Centina', the nutrient amount removed from plant parts of 'Grandgara' and 'Nobless' increased with the sequence of floral part < stem < leaf, indicating that it is more dependent on biomass yield than on the content of nutrients in each plant part. The ratio of N/K amount absorbed by 'Nobless' and 'Centina' was 1.13 and 1.28. respectively, lower than 1.68 of 'Grandgara', showing that the requirement for K is greater in 'Nobless' and 'Centina' than in 'Grandgara'. The use efficiency of nutrients by cut-flower roses ranged from 39 to 64% in nitrogen, 5 to 9% in phosphorus, and 37 to 67% in potassium. It suggests that the requirement for P in cut-flower roses is very low.

• Journal of the Korean Wood Science and Technology
• /
• v.37 no.1
• /
• pp.19-28
• /
• 2009

Some wood anatomical characteristics of seven chestnut cultivars (Tanzawa, Ibuki, Arima, Ginyose, Tsukuba, Riheiguri, and Mansung) grown in Korea were examined in this study. We investigated the radial variation of wood components such as diameter and number of earlywood vessel element and ray density in cross section and number and height of ray in tangential section. Discs were taken from the stem of seven chestnut cultivars at breath height and cut into the radial strip. Cross, radial, and tangential sections, 10 to $20{\mu}m$ thick, were sliced with a sliding microtome. These sections were stained, dehydrated, and mounted with Canada balsam according to common procedure. Measurement and observation were performed with a light microscopy. In all chestnut cultivars, radial and tangential diameter of vessel element increased but number of vessel element per $mm^2$ decreased with increasing tree age. Ray spacing (per mm) in cross section from pith to bark showed no significant variation, while number of ray per $mm^2$ increased but ray height decreased with the increase of tree age. Conclusively, the results obtained in present study were thought to be some valuable information for separating juvenile and adult wood as well as identifying chestnut cultivars.

• Korean Journal of Plant Resources
• /
• v.26 no.4
• /
• pp.503-510
• /
• 2013

This study was conducted to determine the optimum intensity of root pruning and the initial seedling size of 1-year-old Quercus serrata for producing 2-year-old healthy seedlings. Large( > 26 cm hight) and small size ( < 24 cm hight) seedlings were pruned their root by the length of 5, 10, and 15cm and then transplanted on nursery. Stem height, root-collar diameter(RCD), height to RCD ratio(H/D ratio), shoot dry weight(DW) and root development were measured during first growing season after transplanting. Seedling size and pruning intensity influenced on shoot growth such as height, RCD, H/D ratio and DW after transplanting. In addition, the development of lateral roots such as number, length, DW and diameter were affected by the seedling size. Taproot pruned by 15cm was shown excellent above-ground growth in large seedling group, but pruned by 10 and 15cm showed respectively better shoot growth in small seedling group. Large seedlings increased their above-ground growth, taproot regrowth, and number of lateral roots more than those of the small seedlings. But the length and DW of lateral roots were increased in the small seedlings. In conclusion, in order to produce high quality 2-year-old seedlings, it is important to produce large size of Q. serrata seedlings. Also root pruning length of 1-year-old Q. serrata was reasonable on 15 cm in taller than 26 cm or 10cm in less than the height.

• Journal of Korean Society of Forest Science
• /
• v.99 no.3
• /
• pp.337-343
• /
• 2010

The purpose of this study was to understand water stress development in pine seedlings inoculated with Bursaphelenchus xylophilus. Leaf water potentials of four years old Pinus densiflora seedlings inoculated with the nematode were measured with a plant moisture system at predawn and midday. The midday leaf water potentials on the 35th day after the inoculation were -1.04 MPa in the infected seedlings, while -0.94 MPa in the non-infected seedlings. The predawn water potentials on the 56th day were -0.71 MPa in the infected, while -0.26 MPa in the non-infected. At this time the leaves of the infected seedlings became yellow and resin did not exude on the cut basal stem. Density of the nematode was maximum on the 28th day, but dropped sharply on the 68th day. The phloem and pith tissues became brown and the resin exudation reduced in the infected seedlings. In conclusion, pinewood nematode inoculation developed water stress in pine seedlings in a month.

• Korean Journal of Plant Taxonomy
• /
• v.42 no.2
• /
• pp.141-149
• /
• 2012

To clarify some ambiguous diagnostic characters of Callicarpa japonica Thunb. and Callicarpa dichotoma (Lour.) K. Koch in Korea (Verbenaceae), the external morphology and micromorphology (leaf, anther, pollen, fruit, in particular endocarp surface and structure) of two taxa are studied and described in detail. It is confirmed that the following characteristics (e.g., the stem outline in a cross-section, the bud shape and length, the corolla tube length and the lobe length, the pattern of the anther dehiscence and length, and the endocarp morphology) are useful for distinguishing these two taxa. In particular, following three characteristics are most useful for an identification: (1) the corolla tube length of C. japonica (2.5-4.6 mm) is longer than that of C. dichotoma (0.7-1.0 mm); (2) the anther is dehiscing by an apical pore at the anthesis of C. japonica, while it is opening by a long fissure from the apex to the base in C. dichotoma; and (3) the edge of the endocarp (lateral view) in C. japonica is concave, while in C. dichotoma it is shown to be flat. On the other hand, the teeth state on the leaf margin and the position of inflorescence are not good diagnostic characteristics for identification. Additionally, the keys for all known Callicarpa taxa in Korea are provided.

• Polymer(Korea)
• /
• v.29 no.5
• /
• pp.501-507
• /
• 2005

Small intestinal submucosa (SIS) had been widely used as a biomaterial without immune rejection responses. SIS sponges prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). SIS powders dissolved in $3\%(v/v)$ acetic acid aqueous solution for 48hrs and freeze-dried. EDC solution ($H_2O$ : ethanol = 5 : 95) as a crosslink agent was used in concentration of 100mM. In vitro, rat-BMSCs seeded in SIS sponges and induced the osteogenesis for 28 days. We have characterized the osteogenic potential of rat-BMSCs in SIS sponges by alkaline phosphatase activity(ALP), n assay, SEM and RT-PCR for osteogenic phenotype. In SEM, all morphology of SIS sponges was regular and showed interconnected pore structure. By RT-PCR analysis, we observed type I collagen expression. These results demonstrate osteogenic differentiation of rat-BMSCs. In conclusion, we confirmed that the morphology of surface, cross-section, and side of SIS sponges were highly porous with good interconnections between each pores, which can support the surface of cell growth, proliferation, and differentiation. This result indicates that SIS sponge is useful for osteogenesis of BMSCs.

• Korean Journal of Food Science and Technology
• /
• v.42 no.2
• /
• pp.190-197
• /
• 2010

Changes in the quality of buckwheat sprout treated with organic acid solutions such as ascorbic, citric and acetic acid were examined during storage in order to judge the feasibility of acid dipping as a pretreatment for extending shelf-life. Quality attributes of buckwheat sprout stored at $5^{\circ}C$ were measured in terms of microbial cell count, moisture content, soluble solids content, chromaticity, and sensory evaluation over different storage times. For microbial growth inhibition, combinations of 0.05% acetic acid and 0.5% citric acid or 1% ascorbic acid and 0.5% citric acid were more effective than other treatments. Slight changes were observed in moisture content among the treatments, whereas soluble solids content of each treatment was increased during storage. In chromaticity, the Hunter's a value of buckwheat sprout treated with acetic acid alone or acetic acid combinations showed the largest increase. Dipping treatment with ascorbic acid and citric acid produced only slight changes in color of the sprout. In the sensory evaluation of discoloration (head, stem and root), wilting and overall quality, ascorbic acid and citric acid treatments gave the highest scores. These results suggest that dipping treatment of buckwheat sprout with citric acid can confer more positive effects on storage stability than others.

• Korean Journal of Food Science and Technology
• /
• v.10 no.3
• /
• pp.306-312
• /
• 1978

Residue levels of an organochlorine insecticide, BHC, were determined for brown rice samples produced in Korea by collection and field experiments to obtain the following results. 1) Residue levels of total BHC in 112 samples (an Indica type variety Tongil) produced in 1976 and collected from 9 provinces in proportion to the production level of rice ranged from 0.0010 to 0.0762 ppm, the average being 0.0061 ppm. 2) The residue levels in samples from Jeonbook, Kyongnam and Choongnam provinces were higher than the average for whole samples. BHC isomers were found in the order of ${\alpha}>{\gamma}>{\beta}>{\delta}$ isomers, and the detection frequency by range were 89.3% below 0.01 ppm, 9.8% for $0.01{\sim}0.05{\;}ppm$ and 0.9% for $0.05{\sim}0.10{\;}ppm$. 3) In field experiments according to conventional agronomical practices, the brown rice samples after spraying of BHC at the first generation time of rice stem borer (4 kg of 6% granular formulation/10 a paddy field) showed a little higher residue level than control samples, but not more than 0.035 ppm, whereas samples after spraying at the second generation time (3 kg/10 a) contained in the range of $0.04{\sim}0.19\;ppm$.