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Effects of SIS Sponge and Bone Marrow-Derived Stem Cells on the Osteogenic Differentiation for Tissue Engineered Bone  

Park Ki Suk (Department of Polymer Nano Science and Technology, Chonbuk National University)
Jin Chae Moon (Department of Advanced Organic Materials, Chonbuk National University)
Yun Sun Jung (Department of Advanced Organic Materials, Chonbuk National University)
Hong Keum Duck (Department of Advanced Organic Materials, Chonbuk National University)
Kim Soon Hee (Department of Advanced Organic Materials, Chonbuk National University)
Kim Moon Suk (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Rhee John M. (Department of Polymer Nano Science and Technology, Chonbuk National University)
Khang Gilson (Department of Advanced Organic Materials, Chonbuk National University, Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Lee Hai Bang (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Publication Information
Polymer(Korea) / v.29, no.5, 2005 , pp. 501-507 More about this Journal
Abstract
Small intestinal submucosa (SIS) had been widely used as a biomaterial without immune rejection responses. SIS sponges prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). SIS powders dissolved in $3\%(v/v)$ acetic acid aqueous solution for 48hrs and freeze-dried. EDC solution ($H_2O$ : ethanol = 5 : 95) as a crosslink agent was used in concentration of 100mM. In vitro, rat-BMSCs seeded in SIS sponges and induced the osteogenesis for 28 days. We have characterized the osteogenic potential of rat-BMSCs in SIS sponges by alkaline phosphatase activity(ALP), n assay, SEM and RT-PCR for osteogenic phenotype. In SEM, all morphology of SIS sponges was regular and showed interconnected pore structure. By RT-PCR analysis, we observed type I collagen expression. These results demonstrate osteogenic differentiation of rat-BMSCs. In conclusion, we confirmed that the morphology of surface, cross-section, and side of SIS sponges were highly porous with good interconnections between each pores, which can support the surface of cell growth, proliferation, and differentiation. This result indicates that SIS sponge is useful for osteogenesis of BMSCs.
Keywords
small intestinal submucosa; sponge; osteogenesis; BMSCs.;
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