• Ocean and Polar Research
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• 제44권1호
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• pp.29-38
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• 2022

Halophytes are plants that live in harsh environments in coastal regions and are known for their diverse chemical compositions. Limonium tetragonum, a halophyte endemic to Korean shores, is known for its bioactive compounds and is utilized in folk medicine. In this study L. tetragonum extract (LHE) was used to determine and evaluate its anti-osteoporotic properties. Pre-adipocyte and pre-osteoblasts were induced to differentiate along with LHE treatment, and their differentiation was evaluated using differentiation markers. LHE treatment decreased lipid accumulation in 3T3-L1 preadipocytes during adipogenesis. Results indicated that the LHE treatment also decreased the levels of key adipogenic transcription factors: PPARγ, SREBP1c, and C/EBPα. Enhancing osteoblastogenesis by LHE treatment was confirmed in osteoblastogenesis-induced MC3T3-E1 pre-osteoblasts. Cells treated with LHE resulted in increased calcification and alkaline phosphatase (ALP) activity compared with osteoblasts without LHE treatment. Pro-osteogenic and anti-adipogenic effects were also confirmed in D1 murine mesenchymal stromal cells which are capable of differentiation into both adipocytes and osteoblasts. LHE hindered adipogenesis and enhanced osteoblastogenesis in D1 MSCs in a similar fashion. In conclusion, L. tetragonum is believed to possess the potential to be utilized as a nutraceutical ingredient against osteoporotic conditions.

• Clinical and Experimental Reproductive Medicine
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• 제49권2호
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• pp.117-126
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• 2022

Objective: This study examined whether the addition of triple antioxidants (3A)-10 µM acetyl-L-carnitine, 10 µM N-acetyl-L-cysteine, and 5 µM α-lipoic acid-in freezing-thawing medium during human sperm cryopreservation using the sucrose vitrification (SuV) and liquid nitrogen vapor (Vapor) techniques could improve post-thaw survival of spermatozoa. Methods: We analyzed 30 samples from healthy human sperm donors. Each sample was allocated into one of five groups: fresh control, SuV, SuV+3A, Vapor, and Vapor+3A. The sperm motility, morphology, viability, intracellular and extracellular reactive oxygen species (ROS) levels, and sperm DNA fragmentation (SDF) were evaluated. Results: The cryopreserved spermatozoa had significantly reduced percentages of motility (p<0.05) and viability (p<0.05). Antioxidant supplementation non-significantly improved these parameters (p>0.05). No significant differences were found in sperm morphology between the fresh and frozen-thawed groups (p>0.05). After freezing, the extracellular ROS levels in the frozen-thawed groups were significantly higher (p<0.05) than in the fresh group. However, we did not find any differences in intracellular ROS parameters among these groups (p>0.05). The SDF was higher in the SuV and Vapor groups than in the fresh group, but without statistical significance (p=0.075 and p=0.077, respectively). Conclusion: Cryopreservation had detrimental effects on sperm motility, viability, and extracellular ROS levels, without changing the morphology or intracellular ROS levels. Antioxidant supplementation was slightly effective in preventing SDF in frozen-thawed spermatozoa.

• 한국식생활문화학회지
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• 제36권2호
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• pp.143-157
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• 2021

This study aimed to analyze the periodic prevalence of the vegetables during the Joseon era with JoseonWangjoSilrok as a reference. The JoseonWangjoSilrok articles were collected from the Guksapyeonchanwewonhwe site, using web-crawling techniques to extract the relevant information. Out of 384,582 search results, 9,560 articles with vegetable-related keywords were found. According to the annual average vegetable recordings during the regimes of various kings, there were two peaking curves in the 15^th and 18^th centuryJoseon. The found was: 2,750 in the 18^th century, 2,529 in the 15^th century, 1,424 in the 16^th century, and 1,018 in the 19^th century. A Variable Interest Index was designed to ascertain the interestin vegetables of the 27 Joseon kings. The king most interested in vegetables was the 19^th king Sookjong. The second most interested king was Youngjo. There were 5,105 vegetable-related findings within the JoseonWangjoSilrok related to specific species and categories of vegetables. Among the words found: 1,194 were stem-leaves vegetables (23.39%), 1,017 were root vegetables (19.92%), 1,148 were flower-fruit vegetables (22.49%), 1,144 were spice vegetables (22.41%), 95 were mushrooms (1.86%), and 507 were seaweeds (9.93%). Statistical analysis using ANOVA revealed the chronological factors that affected the vegetables' prevalence index.

• 대한본초학회지
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• 제28권1호
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• pp.73-82
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• 2013

Objectives : Provides research basis on steaming processing method (SPM) as described in literatures 'Roegongpojaron', 'Pojadaebub', and 'Susajinam'. Methods : The following issues were considered for current investigation regarding herbal drugs: (i) categorizing SPM-applied drugs, (ii) protocols on the use of the supporting materials, (iii) kind of supporting materials, (iv) processing periods, (v) part of the herb plants, (vi) herbal efficacy, (vii) intrinsic properties and tastes of the herbal drugs, (viii) meridian tropism of the herbal drugs. Result : The number of herbal drugs was 70 species from 'Roegongpojaron', 73 from 'Pojadaebub', and 66 from 'Susajinam'. The abundance of supporting materials was in the order of alcohol, honey and tofu. The herbal parts of the roots, stem, fruit, and seed were most widely used. Based on herbal efficacy, a drug supplementing invigoration was used most, and a drug eliminating heat followed next. Based on four spirit features, herbs showing warm, cold, and mild features were used. In considering five tastes, herbs showing bitter and sweet tastes were used most. In considering meridian tropism, herbs converging to liver and kidneys were used, and drugs converging to 'Samcho, and gallbladder were not used. Conclusion : The present survey on SPM as described in 'Roegongpojaron', Pojadaebub', and 'Susajinam' indicates that there is a principal specialty on the use of supporting materials, steaming periods, steaming parts and efficacy.

• Nutrition Research and Practice
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• 제16권4호
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• pp.435-449
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• 2022

BACKGROUND/OBJECTIVES: Cheonggukjang is a traditional fermented soybean paste with significant health-promoting effects. On the other hand, there have been insufficient studies on the safety and efficacy of Cheonggukjang, which is produced using traditional methods containing toxins and biogenic amines (BAs). This study compared the laxative effect of Cheonggukjang, containing high or low levels of toxins and BAs (HTBC or LTBC) in a loperamide (Lop)-induced constipation mouse model. MATERIALS/METHODS: To induce constipation, Lop (5 mg/kg) was administered orally to ICR mice twice a day for 4 days, and the dose was increased to 8 mg/kg after a 3-day rest period. Cheonggukjang (500 mg/kg, HTBC, or LTBC respectively) was administered for four weeks before the Lop treatment. RESULTS: The number of stools, fecal weight, water contents, gastrointestinal transit, and histological alterations were recovered significantly in the HTBC or LTBC groups. HTBC and LTBC administration did not induce significant changes in body weight, dietary intake, and behavior. The opioid-receptor downstream signaling pathway in colon tissues was also evaluated. The c-Kit, stem cell kinase, and mitogen-activated protein kinases subfamilies, including extracellular signal-regulated kinase 1/2, c-Jun N-terminal kinases, and p38, were all downregulated in the HTBC or LTBC-administered mice colon compared to the Lop group. CONCLUSION: These results show that Cheonggukjang, containing high levels of toxins and BAs, have a similar laxative effect in a mouse model of Lop-induced constipation.

• 한국환경과학회지
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• 제30권11호
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• pp.957-965
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• 2021

This research was carried out to investigate seed germination and growth of the perennial plant Apocynum lancifolium under different NaCl concentrations, with a view for future cultivation on reclaimed land. Initial characterization revealed that the average length and weight of A. lancifolium seed pods was 133.6 mm and 0.23 g, respectively, and the thousand-grain weight was 0.59 g. Upon examining the effects of light on seed germination, we found germination to be 1.7% higher under light conditions (90%) than under dark conditions (88.3%). In terms of the response to salt stress, we found that 90% of seeds germinated in the 0.00%, 0.25%, and 0.50% salt treatment groups. Although salt treatment up to a concentration of 0.5% was found to have little effect on seed germination, the rate of germination decreased at higher concentrations and was completely inhibited in the 2% treatment. We also established that germination rates were higher in seeds sown in horticultural topsoil than in the coarse sandy soil found in the plant's natural habitats. Although the growth of A. lancifolium tends to decrease with an increase in salt concentration, we found that the stem thickness, fresh weight, and dry weight of A. lancifolium seedlings subjected to 0.25%-1.0% salt were comparable to those of the control seedling that were not exposed to salt. Furthermore, in contrast to those plants subjected to 2.0% salt, these plants continued to grow and remained viable.

• 한국수학교육학회지시리즈E:수학교육논문집
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• 제36권3호
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• pp.439-467
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• 2022

미래 사회에는 지식뿐만 아니라 창의성과 협동심, 융합적 사고 등을 포함하는 다양한 역량이 필요하다. 본 연구는 중요한 수학 교과 역량인 수학 문제해결력, 의사소통 능력 등의 함양을 기대하며 수학 정보과학 융합을 위한 프로그램을 개발하였다. 선행지식이 크게 요구되지 않고, 일상언어와 쉽게 접할 수 있는 도구만으로 동기유발이 가능하며 다자간 협력이 필수적인 창의적 문제해결 활동 기반 프로그램이다. 활동의 참가자 수가 증가함에 따라 수학의 유용성과 엄밀성을 경험할 수 있으며, 이론적 원리는 유한체 위에서의 행렬 이론을 바탕으로 한다. 또한 정보과학에서 주요 주제 중 하나인 오류정정코드와의 관련성을 강조할 수 있도록 구성하였다. 본 프로그램의 실세계 맥락이 수학적 의사소통 능력의 함양과 수학의 가치 경험 기회 제공에 도움이 되기를 바라고, 코딩을 수반하지 않는다는 점에서 교사들의 접근성이 높기를 기대한다.

• BMB Reports
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• 제55권6호
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• pp.281-286
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• 2022

Hepatocellular carcinoma is a major health burden, and though various treatments through much research are available, difficulties in early diagnosis and drug resistance to chemotherapy-based treatments render several ineffective. Cancer stem cell model has been used to explain formation of heterogeneous cell population within tumor mass, which is one of the underlying causes of high recurrence rate and acquired chemoresistance, highlighting the importance of CSC identification and understanding the molecular mechanisms of CSC drivers. Extracellular CSC-markers such as CD133, CD90 and EpCAM have been used successfully in CSC isolation, but studies have indicated that increasingly complex combinations are required for accurate identification. Pseudogene-derived long non-coding RNAs are useful candidates as intracellular CSC markers - factors that regulate pluripotency and self-renewal - given their cancer-specific expression and versatile regulation across several levels. Here, we present the use of microarray data to identify stemness-associated factors in liver cancer, and selection of sole pseudogene-derived lncRNA ZNF204P for experimental validation. ZNF204P knockdown impairs cell proliferation and migration/invasion. As the cytosolic ZNF204P shares miRNA binding sites with OCT4 and SOX2, well-known drivers of pluripotency and self-renewal, we propose that ZNF204P promotes tumorigenesis through the miRNA-145-5p/OCT4, SOX2 axis.

• 현장농수산연구지
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• 제14권1호
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• pp.119-130
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• 2012

상추의 채종효율을 증진하기 위해서는 잎상추의 경우는 14엽기에 GA₃ 20mg/ℓ, 결구상추의 경우 혼합처리인 8+14엽기의 GA₃ 20mg/ℓ처리가 우수한 것으로 나타났다. GA₃처리에 의한 고사율의 감소로 채종량이 엽상추는 50.9 → 14엽기 61.7ℓ/10a로 21% 증수 할 수 있었으며, 결구상추는 그 효과는 적었으나 8+14엽기에 GA₃ 20mg/ℓ처리시 무처리에 비해 14.2 →21.1ℓ/10a로 14.9% 증수효과가 있었다. 특히 결구상추 채종을 위해서는 6월 이후에 파종하는 것이 무름병과 균핵병에 의한 부패율을 경감시킬 수 있었다.

• 한국응용곤충학회지
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• 제25권1호
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• pp.37-40
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• 1986

필리핀에 있는 국제미작연구소(國際米作硏究所) 논 포장(圃場)에서 이앙(移秧)후 $15{\sim}20$일(日) 된 묘(苗)에서 Scirpophaga incertulas의 난(卵)을 1984년(年) 7월 부터 10월 까지 채집(採集)하여 난기생봉(卵寄生蜂)의 기생율(寄生率) 및 생태(生態)를 조사한 결과(結果)는 다음과 같다. ScirpoPhaga incertulas 의 난(卵)에서 나온 벌목 천적(天敵)으로 Tetrasticus schoenobii, Telenomus rowani, Trichogramma japonicum 3종(三種)이 발견(發見)되었고 기생봉(寄生蜂)의 생물적(生物的)인 습성(習性)에 기초(基礎)를 둔 기생율(寄生率) 계산(計算)방법을 소개하였다. 난기생봉(卵寄生蜂) 3종(三種)의 공기생(共寄生) 조합(組合)은 T. rowani+T. japonicum 조합(組合)이 가장 많았고 T. rowani 는 단기생성(單寄生性) 천적(天敵)으로 우화성충(羽化成蟲) 수(數)가 가장 많았지만 3종(三種)의 기생봉중(寄生蜂中) T. schoenobii 는 유충기간(幼蟲期間) 중 Scirpophaga incertulas 의 난(卵)을 $2{\sim}4$개(個) 소모(消耗)시키므로 가장 유용(有用)한 천적(天敵)이었으며 이 천적(天敵)의 1세대기간(世代期間)은 $10{\sim}14$일(日) 이었다.