Browse > Article
http://dx.doi.org/10.5483/BMBRep.2022.55.6.001

ZNF204P is a stemness-associated oncogenic long non-coding RNA in hepatocellular carcinoma  

Hwang, Ji-Hyun (Interdisciplinary Program of Integrated OMICS for Biomedical Science, The Graduate School, Yonsei University)
Lee, Jungwoo (Interdisciplinary Program of Integrated OMICS for Biomedical Science, The Graduate School, Yonsei University)
Choi, Won-Young (Interdisciplinary Program of Integrated OMICS for Biomedical Science, The Graduate School, Yonsei University)
Kim, Min-Jung (Interdisciplinary Program of Integrated OMICS for Biomedical Science, The Graduate School, Yonsei University)
Lee, Jiyeon (Severance Biomedical Science Institute, Graduate School of Medical Science, Brain Korea 21 Project, Gangnam Severance Hospital, Yonsei University College of Medicine)
Chu, Khanh Hoang Bao (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
Kim, Lark Kyun (Severance Biomedical Science Institute, Graduate School of Medical Science, Brain Korea 21 Project, Gangnam Severance Hospital, Yonsei University College of Medicine)
Kim, Young-Joon (Interdisciplinary Program of Integrated OMICS for Biomedical Science, The Graduate School, Yonsei University)
Publication Information
BMB Reports / v.55, no.6, 2022 , pp. 281-286 More about this Journal
Abstract
Hepatocellular carcinoma is a major health burden, and though various treatments through much research are available, difficulties in early diagnosis and drug resistance to chemotherapy-based treatments render several ineffective. Cancer stem cell model has been used to explain formation of heterogeneous cell population within tumor mass, which is one of the underlying causes of high recurrence rate and acquired chemoresistance, highlighting the importance of CSC identification and understanding the molecular mechanisms of CSC drivers. Extracellular CSC-markers such as CD133, CD90 and EpCAM have been used successfully in CSC isolation, but studies have indicated that increasingly complex combinations are required for accurate identification. Pseudogene-derived long non-coding RNAs are useful candidates as intracellular CSC markers - factors that regulate pluripotency and self-renewal - given their cancer-specific expression and versatile regulation across several levels. Here, we present the use of microarray data to identify stemness-associated factors in liver cancer, and selection of sole pseudogene-derived lncRNA ZNF204P for experimental validation. ZNF204P knockdown impairs cell proliferation and migration/invasion. As the cytosolic ZNF204P shares miRNA binding sites with OCT4 and SOX2, well-known drivers of pluripotency and self-renewal, we propose that ZNF204P promotes tumorigenesis through the miRNA-145-5p/OCT4, SOX2 axis.
Keywords
Cancer stemness; lncRNA; Liver cancer; Pseudogene; ZNF204P;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Nio K, Yamashita T, and Kaneko S (2017) The evolving concept of liver cancer stem cells. Mol Cancer 16, 4   DOI
2 Yang L, Shi P, Zhao G et al (2020) Targeting cancer stem cell pathways for cancer therapy. Signal Transduct Target Ther 5, 8   DOI
3 Kim WT and Ryu CJ (2017) Cancer stem cell surface markers on normal stem cells. BMB Rep 50, 285-298   DOI
4 Chen S, Zhu J, Wang F et al (2017) LncRNAs and their role in cancer stem cells. Oncotarget 8, 110685-110692   DOI
5 Statello L, Guo CJ, Chen LL, and Huarte M (2021) Gene regulation by long non-coding RNAs and its biological functions. Nat Rev Mol Cell Biol 22, 96-118   DOI
6 Yan X, Hu Z, Feng Y et al (2015) Comprehensive genomic characterization of long non-coding RNAs across human cancers. Cancer Cell 28, 529-540   DOI
7 Yndestad S, Austreid E, Skaftnesmo KO, Lonning PE, and Eikesdal HP (2018) Divergent activity of the pseudogene PTENP1 in ER-positive and negative breast cancer. Mol Cancer Res 16, 78-89   DOI
8 Lou WY, Ding BS, and Fu PF (2020) Pseudogene-derived lncRNAs and their miRNA sponging mechanism in human Cancer. Front Cell Dev Biol 8, 85   DOI
9 Chiba T, Miyagi S, Saraya A et al (2008) The polycomb gene product BMI1 contributes to the maintenance of tumor-initiating side population cells in hepatocellular carcinoma. Cancer Res 68, 7742   DOI
10 Zhang J, Luo N, Luo Y, Peng Z, Zhang T, and Li S (2012) microRNA-150 inhibits human CD133-positive liver cancer stem cells through negative regulation of the transcription factor c-Myb. Int J Oncol 40, 747-756   DOI
11 Jia Y, Liu H, Zhuang Q et al (2012) Tumorigenicity of cancer stem-like cells derived from hepatocarcinoma is regulated by microRNA-145. Oncol Rep 27, 1865-1872   DOI
12 Lian Q, Wang S, Zhang G et al (2018) HCCDB: a database of hepatocellular carcinoma expression atlas. Genomics Proteomics Bioinformatics 16, 269-275 (Available from: http://lifeome.net/database/hccdb/download.html)   DOI
13 Gui X, Li H, Li T, Pu H, and Lu D (2015) Long noncoding RNA CUDR regulates HULC and β-Catenin to govern human liver stem cell malignant differentiation. Mol Ther 23, 1843-1853   DOI
14 Li H, An J, Wu M et al (2015) LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget 6, 27847-27864   DOI
15 Shi J, Levinson DF, Duan J et al (2009) Common variants on chromosome 6p22.1 are associated with schizophrenia. Nature 460, 753-757   DOI
16 Morelli E, Gulla A, Amodio N et al (2021) CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) to explore the oncogenic lncRNA network. Methods Mol Biol 2348, 189-204   DOI
17 Hon C-C, Ramilowski JA, Harshbarger J et al (2017) An atlas of human long non-coding RNAs with accurate 5' ends. Nature 543, 199-204   DOI
18 Lamb JR, Zhang C, Xie T et al (2011) Predictive genes in adjacent normal tissue are preferentially altered by sCNV during tumorigenesis in liver cancer and may rate limiting. PLoS One 6, e20090   DOI
19 Qin XY, Suzuki H, Honda M et al (2018) Prevention of hepatocellular carcinoma by targeting MYCN-positive liver cancer stem cells with acyclic retinoid. Proc Natl Acad Sci U S A 115, 4969-4974   DOI
20 Takahashi K, Tanabe K, Ohnuki M et al (2014) Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. Nat Commun 5, 3678   DOI
21 Chandrashekar DS, Bashel B, Balasubramanya SAH et al (2017) UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia (New York, N.Y.) 19, 649-658   DOI
22 Lu TP, Lee CY, Tsai MH et al (2012) miRSystem: an integrated system for characterizing enriched functions and pathways of microRNA targets. PLoS One 7, e42390   DOI
23 Zou S, Wang C, Liu J et al (2017) Sox12 is a cancer stem-like cell marker in hepatocellular carcinoma. Mol Cells 40, 847-854   DOI
24 Xie Z, Bailey A, Kuleshov MV et al (2021) Gene set knowledge discovery with enrichr. Curr Protoc 1, e90
25 Liberzon A, Birger C, Thorvaldsdottir H, Ghandi M, Mesirov JP, and Tamayo P (2015) The molecular signatures database (MSigDB) hallmark gene set collection. Cell Syst 1, 417-425   DOI
26 Labun K, Montague TG, Krause M, Torres Cleuren YN, Tjeldnes H, and Valen E (2019) CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Res 47, W171-W174   DOI
27 Zhang H and Wang ZZ (2008) Mechanisms that mediate stem cell self-renewal and differentiation. J Cell Biochem 103, 709-718   DOI
28 Shi G and Jin Y (2010) Role of Oct4 in maintaining and regaining stem cell pluripotency. Stem Cell Res Ther 1, 39   DOI
29 European Association for the Study of the Liver (2018) EASL Clinical Practice Guidelines: management of hepatocellular carcinoma. J Hepatol 69, 182-236   DOI
30 Ginestier C, Hur MH, Charafe-Jauffret E et al (2007) ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome. Cell Stem Cell 1, 555-567   DOI
31 Fatica A and Bozzoni I (2014) Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet 15, 7-21   DOI
32 Xu Y, Yu X, Wei C, Nie F, Huang M, and Sun M (2018) Over-expression of oncigenic pesudogene DUXAP10 promotes cell proliferation and invasion by regulating LATS1 and β-catenin in gastric cancer. J Exp Clin Cancer Res 37, 13   DOI
33 Tsui YM, Chan LK, and Ng IOL (2020) Cancer stemness in hepatocellular carcinoma: mechanisms and translational potential. Br J Cancer 122, 1428-1440   DOI
34 Xu N, Papagiannakopoulos T, Pan G, Thomson JA, and Kosik KS (2009) MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell 137, 647-658   DOI
35 Lin H, Qiu X, Zhang B, and Zhang J (2018) Identification of the predictive genes for the response of colorectal cancer patients to FOLFOX therapy. OncoTargets Ther 11, 5943-5955   DOI