• Journal of Acupuncture Research
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• v.15 no.2
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• pp.287-300
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• 1998

The purpose of this study was finding the pain inhibitory effect of acupuncture at ST-36. The pain at dentes incisor was evoked by noxious electric stimulation and EMG changes based on time interval were measured. To do this, the opioid antagonist was administered intraperitoneally. Followings are some of the results. 1. The pain inhibitory effect of acupuncture at ST-36 was 93.82%, 87.00%, 75.30%, 69.76% at 10 20, 30 and 40 minutes respectively. All the data were calculated based on 100% at 0 minute. The effect was very significant at 40 minutes. 2. The continuous effect of acupuncture at ST-36 after taking out the needle was 77.46%, 79.66%, 87.60%, 91.50%, 95.14%, 99.48% at 50, 60, 70, 80, 90 and 100 minutes respectively. The overall continuous effect was shown its maximum 20 minutes after taking out the needle and reached to the initial value at 60 minutes. 3. The pain inhibitory effect following the naloxone administration and acupuncture application ST-36 was 93.44%, 94.58%, 90.80%, 88.04% at 10, 20, 30 and 40 minutes respectively. All values showed similar tendency to the initial data. 4. The continuous effect of acupuncture at ST-36 after taking out the needle which was preceded by the naloxone administration was 91.26%, 91.90%, 92.06%, 93.66%, 94.12%, 93.50% at 50, 60, 70, 80, 90 and 100 minutes respectively. This trend was not a significant fluctuation. It Was concluded that effect of acupuncture at ST-36 was shown in conjunction with secretion of analgesic substance. Therefore it is implied that the acupuncture application will play a major role in treating many diseases with more revelation of scientific acupuncture mechanism. Further studies of acupuncture manipulation are needed in the future based on our study.

• Journal of Acupuncture Research
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• v.23 no.6
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• pp.207-219
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• 2006

Objectives : This study was to investigate the effect of low frequency electro-acupuncture (EA) at ST36 acupoint on the Freund's Complete Adjuvant(FCA)-induced arthritis in rats. Methods : Arthritis was induced by intradermal injection of FCA into base of tail. Experimental groups were divided into 4 groups; Normal, Control, ST36 and Non-Acupoint(NA). ST36 group was treated by 2 Hz EA at ST36 acupoint. Body weight, paw edema volun1e and ankle joint thickness were measured after treatment. And we investigated the effects of 2 Hz EA via WBC count, segment neurophil, lymphocyte, PGE2 assay and NADPH-d histochemistry. Results and Conclusion : The mean of body weight and ankle joint thickness of ST36 group was increased compared with control group. The mean of paw edema volume, WBC count, segment neutrophil and lymphocyte of were not decreased with control group. The mean of PGE2 concentration and NADPH-d positive neurons were changed significantly compared with control group.

• Journal of Acupuncture Research
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• v.21 no.6
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• pp.167-176
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• 2004

Objective : This study was designed to investigate the effects of electro-acupuncture(EA) and moxibustion at CV13, CV12, CV10 and ST36 serum gastin level in rats. Methods : The present study was conducted to see the effects produced by combined electro-acupuncture(EA) and moxibustion using four different acupoints on serum gastrin level in rats. Electro-acupuncture (2 Hz, intensity ; 10 times muscle twitch threshold) was applied for 30 minutes to ST36 under halothane anesthesia. Moxibustion ($1.6{\pm}0.3mg$, 5 times of a day) was applied during 3 days to CV13, CV12, CV10 under halothane anesthesia. Results : Both of EA applied to the ST 36 and Moxibustion applied to the CV10, CV12 decreased serum gastrin level significantly. And the effect of combined EA at ST36 and moxibustion at CV10 was shown stronger effect than each singular stimulus of EA at ST36 and moxibustion at CV10. These data suggest that both of EA and moxibustion at specific acupoints produce change of serum gastrin level in rats and combined EA and moxibustion was shown quite effect than each singular stimulus.

• Journal of Acupuncture Research
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• v.29 no.2
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• pp.1-8
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• 2012

Objectives : This study was performed to compare the effects between Mountain-burning fire method and Heaven-penetrating cooling method on thermographic change. Materials and Methods : The experiment was conducted to 20 students of College of Oriental Medicine, Woosuk University from July 12, 2011 to July 26, 2011. Acupuncture stimulation was applied at left $Zusanli$($ST_{36}$) with Mountain-burning fire method in A group(n=10) and Heaven-penetrating cooling method in B group (n=10). To estimate thermographic change, temperature of left $Zusanli$($ST_{36}$), $Shuigou$($GV_{26}$), $Zhongwan$($CV_{12}$) were measured by DITI(digital infrared thermographic image) before acupuncture stimulation and 30sec, 10min after needle removal. Results : In A group, the temperature of left $Zusanli$($ST_{36}$), $Shuigou$($GV_{26}$), $Zhongwan$($CV_{12}$) were decreased at 30sec after needle removal, statistically. And the temperature of $Zusanli$($ST_{36}$), $Zhongwan$($CV_{12}$) were decreased at 10min after needle removal, statistically. In B group, the temperature of $Zusanli$($ST_{36}$), $Zhongwan$($CV_{12}$) were decreased at 10min after needle removal, statistically. But, there was no significant difference between two groups on thermographic change, statistically Conclusions : Acupuncture stimulation applied at left $Zusanli$($ST_{36}$) with Mountain-burning fire method has more widespread effects than Heaven-penetrating cooling method. But further studies will be needed such as thermographic change with no stimulation and with more subjects.

• Journal of Acupuncture Research
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• v.25 no.1
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• pp.25-55
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• 2008

Objectives : The aim of the present study was to observe the effect of Astragali Radix Herbal-Acupuncture Solution(AR-HAS) at $ST_{36}$(jok-samni, $Z\acute{u}s\bar{a}n$ Li) on collagen- II -induced arthritis(CIA) in mice. Methods : DBA/1J mice were immunized with bovine type II collagen(CII) on days 0 and 21 to induce arthritis. The mice were divided into 5 groups : normal group(no CIA), control group(CIA+no treatment), needle prick group(CIA+single prick with an injection needle), saline group(CIA+saline injection) and ARHA group(CIA+ R-HA treatment). The needle prick, saline injection, and AR-HA groups were injected on the right $ST_{36}$(jok-samni) of mice for 9 weeks, 3 times a week, beginning 4 weeks after the booster immunization. Results : 1. The incidence of arthritis, AI(arthritis index), and joint edema decreased in the AR-HA group. 2. Weight gain, hypertrophy of the spleen, adhesion of the tissues, and transformation of the joint were restrained in the AR-HA group. 3. The concentrations of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, $IFN-{\gamma}$ in CIA mouse serum and $IFN-{\gamma}$, IL-10 in the CIA mouse spleen cell culture decreased significantly at $ST_{36}$ in the AR-HA group. 4. Total cell counts increased significantly, and the ratio of $CD3e^+$ to $CD45R^+$, $CD4^+$ to $CD8^+$, and $CD4^+$ to $CD25^+$ cells decreased significantly at $ST_{36}$ in the CIA mouse spleen cell culture of the AR-HA group. 5. Total cell counts decreased significantly, and $CD4^+/CD25^+$ and $CD45R^+/CD69^+$ decreased significantly at $ST_{36}$ in the CIA mouse lymph nodes of the AR-HA group. 6. $CD3e^+/CD69^+$ and $CD11b^+/Gr-1^+$ cells decreased significantly at $ST_{36}$ in the CIA mouse joints of the AR-HA group. 7. The histological examination showed that cartilage destruction and synoviocyte proliferation decreased in the CIA mouse joints of the AR-HA group, and collagen fiber was expressed similar to that seen in the normal group. Conclusions : Our experiments show that at $ST_{36}$, an anti-inflammatory mechanism of AR-HA controls synovial cell proliferation and protects against cartilage destruction in rheumatoid arthritis.

• Journal of Acupuncture Research
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• v.17 no.2
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• pp.139-152
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• 2000

This study was designed to evaluate the analgesic effect of bee venom (BV) Acupuncture into different treatment points, Chok-samni (ST36) and blank loci of the gluteal muscle and back. We investigated the changes in formalin-induced pain behavior according to the pretreatment with different concentrations of BV, thirty minutes before the formalin injection. The results were summarized as follows: 1. The formalin-induced pain behavior was suppressed by pretreatment with BV into Chok-samni (ST36) in a dose dependent manner. During the early phase, 0.08mg/kg of BV showed a statistically significant suppression in the formalin-induced pain behavior. Moreover, 0.008mg/kg, 0.016mg/kg, and 0.08mg/kg of BV, except 0.0016mh/kg of BV, had significant suppresive effects on the formalin-induced pain behavior during the late phase. Therefore, these data indicated that the suppressive effect of BV acupuncture on the formalin-induced pain behavior was stronger in the late phase rather than the early phase 2. In order to investigate the analgesic effect of BV acupuncture into different treatment points, the experimental animals were divided into three groups: Chok-samni (ST36) group, gluteal group and back group. In the Chok-samni (ST36) group, the formalin-induced pain behavior during all the phases was significantly reduced as compared with that of the back group. However, as compared with that of the gluteal group, the formalin-induced pain behavior in the Chok-samni (ST36) group was decreased only in the late phase, not in the early phase. The formalin-induced pain behavior in the gluteal group was significantly reduced as compared with that of the back group in the late phase, not in the early phase. We suggested that the analgesic effect of BV acupuncture into Chok-samni (ST36) was most effective among Chok-samni (ST36), gluteal, and the back groups in formalin-induced pain behavior.

• Journal of Acupuncture Research
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• v.25 no.4
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• pp.95-103
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• 2008

Objectives : This study was to investigate the regulatory effect of electro-acupuncture on Jogsamni ($ST_{36}$) in bradygastric condition or gastroparesis which is a neuropathic complication of type 2 diabetes mellitus(T2DM), assessed using 4-channel electrogastrography (EGG). Methods : It was a case series study. Each patient was given electro-acupuncture(EA) one point, Jogsamni($ST_{36}$) or Gyeonu($LI_{15}$) bilaterally for thirty minutes with 2Hz bipolar square wave frequency and moderate intensity of stimulation. With attaching electrodes around stomach region throughout pre-acupuncture, acupuncture, and post-acupuncture session, these parameters were produced; percent rate of bradygastria power compared to sum of power in all kind of gastric rhythm(% bradygastria), dominant frequency(DF) and dominant power(DP). Parameter was analysed per each channel's variable change from pre-acupuncture session by paired t-test. Results : There was statistically significant decrease in % bradygastria parameters on one of the $ST_{36}$ treated patients from pre-acupuncture session and to post-acupuncture session(P=0.015, P=0.008 respectively). A marginal significant decrease of DP was shown in the other $ST_{36}$ treated patient from pre-acupuncture session through post-acupuncture session(P=0.049). Combined data of two $ST_{36}$ Treated patients showed that there was significant decrease of %bradygastria from pre-acupuncture session to acupuncture session(P=0.020), and was decrease of DP from pre-acupuncture session to post- acupuncture session(P=0.020). Conclusions : EA at $ST_{36}$ resulted in statistically significant decrease in %bradygastria and DP for dysrhythmic condition in T2DM gastroparesis patients. Considering this limited results, further study will be needed to elucidate the effect of multiple-acupuncture on gastric myoelectrical activities.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.672-676
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• 2003

To investigate the effect of acupuncture on Zusanli (ST 36) in this study, gastrin level of serum by radioimmunoassay was measured at 1, 3, 6 weeks in rats. In other groups, 1 mg/day omeprazole was administered for 1, 3, 6 weeks. Omeprazole increased significantly gastrin level of serum at 1, 3, 6 weeks in a time dependent manner. Acupuncture applied to the ST 36 acupoint decreased at 1, 3, 6 weeks. Furthermore, acupuncture applied to other acupoint, Yanglinquan (GB 34) did not produced significant effect. When the common peroneal nerve was dissected, acupuncture of ST 36 produced the change of gastrin level. These data suggest that acupuncture decreased gastrin level of serum in point specific way and that effect was not related with surrounding nerve.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1276-1280
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• 2003

To investigate the effect of acupuncture at Zushanli (ST 36) in this study, gastric endocrine cells (G cell) by avidin-biotinylated complex (ABC) technique and histological examinations (HE; periodic acid schiff, PAS; alcian blue stain) of the stomach were perfomed at 1, 3, 6 weeks in normal rats. In other groups, omeprazole were fed for 1, 3, 6 weeks to compare with acupuncture effect. Acupuncture applied to the ST 36 acupoint and the administration of omeprazole increased G cell significantly at 1, 3, 6 weeks in time dependant manner. Furthermore, acupuncture applied to the other acupoint on GB 34 did not produce significant effect. When the common peronial nerve was dissected, acupuncture of ST 36 acupoint produced change of G cell. These data suggest that acupuncture at ST 36 increased G cell in point specific way and that effect was not related with surrounding nerve.

• Korean Journal of Acupuncture
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• v.26 no.2
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• pp.53-60
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• 2009

Objectives: To investigate the effects of Hominis placenta pharmacopuncture on the blood picture and antioxidative activity in rats. Methods: Sprague-Dawley rats were divided into 3 groups; normal control (n=5), pharmacopuncture at CV12 (CV12 group, n=5), and pharmacopuncture at ST36 (ST36 group, n=5) once every other day for 4 weeks. Blood cell counting was performed and liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were analyzed. Results: Values of red blood cell and plasma cell volume were significantly higher in the ST36 group than the normal control. Values of hematocrit, total protein, and albumin were not significantly different among groups. White blood cell count and the percentage of neutrophils, lymphocytes, and eosinophils were not significantly different among groups. However, monocytes and basophils were significantly increased in the ST36, and CV12 groups, respectively. SOD and CAT in the CV12 and ST36 groups were significantly activated than in the normal control group, while the activity of GSH-Px showed no significant difference among groups. Conclusions: Based on the findings of this study, Hominis placenta pharmacopuncture may have positive impact on antioxidative capacity, thus activate various functions of the body.