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Dry Matter Yield, Growth Characterics and Nutritive Value of Wild alfalfa (야생 알팔파의 건물수량, 생육특성 및 사료적인 가치)

  • Shin, C.N.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.155-158
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    • 1999
  • Alfalfa grows wild in some parts of Korea, but specific information is lacking as to its growth characteristics, nutritive value and dry matter yeild. The objective of this study was to evaluate the usefulness of wild alfalfa(Medicago sativa L.) as a forage. Wild alfalfa and 'Vernal' were field sown at Keongsan, Keongbuk in the spring of 1995. Emergence for 'Vernal' was better than for wild alfalfa. It was observed that the flowering date of the wild alfalfa was delayed by 8 days. Regrowth of 'Vernal' was better than that of the wild alfalfa at each harvesting. After the last harvesting date, September 22, there was poor regrowth of the wild alfalfa, but regrowth of 'Vernal' was moderately vigorous growth. Weed infestation in the wild alfalfa plots was higher than in the 'Vernal' plots. Thle dry matter yields per hectare were significantly(P<0.05) higher for 'Vernal' than for the wild alfalfa. The CP content was slightly higher in the wild alfalfa in comparison to 'Vernal'. NDF and ADF contents were lower in the third cut, but they were not different in the 1st and 2nd cuttings.

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Alteration of Immunoreactivity for SNARE Proteins in the Rat Hippocampus after Middle Cerebral Artery Occlusion

  • Park, Jung-Sun;Huh, Pil-Woo;Jung, Yeon-Joo;Park, Su-Jin;Lee, Kyung-Eun
    • The Korean Journal of Physiology and Pharmacology
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    • v.8 no.3
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    • pp.141-146
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    • 2004
  • Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins, composed of two presynaptic membrane proteins [synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin] and a presynaptic vesicular protein [vesicle-associated membrane protein (VAMP)], serve as a core of exocytotic fusion machinery, which can be affected by ischemia. Synaptic protein in core region, striatum and cortex has been shown to alter after focal ischemia, however, little is known in hippocampus. Hippocampus is remote from ischemic core, but it is one of the most vulnerable regions. Using immunohistochemistry, the present study was undertaken to investigate the alteration of expression of SNAP-25, syntaxin, and VAMP in the hippocampus of rats which were subjected to middle cerebral artery occlusion (MCAO) for 2h and allowed to reperfuse. At 2 weeks of reperfusion, the SNAP-25 and syntaxin immunoreactivity was increased in the stratum oriens of the CA1 and the stratum lucidum of the CA3 in the ipsilateral hippocampus. However, VAMP immunoreactivity didn't show significant change. These results demonstrate that the level of the presynatpic plasma membrane proteins (SNAP-25 and syntaxin) in the rat hippocampus is more sensitively affected by focal ischemia than that of the synaptic vesicle protein (VAMP).

Expression and Characterization of Human N-Acetylglucosaminyltransferases and ${\alpha}$2,3-Sialyltransferase in Insect Cells for In Vitro Glycosylation of Recombinant Erythropoietin

  • Kim, Na-Young;Kim, Hyung-Gu;Kim, Yang-Hyun;Chung, In-Sik;Yang, Jai-Myung
    • Journal of Microbiology and Biotechnology
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    • v.18 no.2
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    • pp.383-391
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    • 2008
  • The glycans linked to the insect cell-derived glycoproteins are known to differ from those expressed in mammalian cells, partly because of the low level or lack of glycosyltransferase activities. GnT II, GnT IV, GnT V, and ST3Gal IV, which play important roles in the synthesis of tetraantennarytype complex glycan structures in mammalian cells, were overexpressed in Trichoplusia ni cells by using a baculovirus expression vector. The glycosyltransferases, expressed as a fusion form with the IgG-binding domain, were secreted into the culture media and purified using IgG sepharose resin. The enzyme assay, performed using a pyridylaminated-sugar chain as an acceptor, indicated that the purified glycosyltransferases retained their enzyme activities. Human erythropoietin expressed in T. ni cells (rhEPO) was subjected to in vitro glycosylation by using recombinant glycosyltransferases and was converted into complex-type glycan with terminal sialic acid. The presence of Nacetylglucosamine, galactose, and sialic acid on the rhEPO moiety was detected by a lectin blot analysis, and the addition of galactose and sialic acid to rhEPO was confirmed by autoradiography using $UDP-^{14}C-Gal\;and\;CMP-^{14}C-Sia$ as donors. The in vitro glycosylated rhEPO was injected into mice, and the number of reticulocytes among the ed blood cells was counted using FACS. A significant increase in the number of reticulocytes was not observed in the mice injected with in vitro glycosylated rhEPO as compared with those injected with rhEPO.

The Mediating Effect of Ego-resilience on the Relation between Parental Attitude Perceived by Adolescence and Self-Regulated Learning Ability (청소년이 지각하는 부모양육태도와 자기조절학습능력과의 관계에서 자아탄력성의 매개효과)

  • Kim, Yoo-Lee
    • The Journal of the Korea Contents Association
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    • v.18 no.10
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    • pp.596-607
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    • 2018
  • The purpose of this study was to review the relationships among parental care and parental overprotection perceived by adolescence, their ego-resilience and self-regulated learning ability. For this purpose, a total 2,002 students were selected from the seventh data sets of the Korean Child and Youth Panel Survey(KCYPS). More specifically, samples were composed of 1st graders in middle schools(n=2,002). As a result, first, the perceived parental care had significant effects on the self-regulated learning ability both directly and indirectly through ego-resilience. Second, the perceived parental overprotection had effects on their self-regulated learning ability mediated by ego-resilience. But the effect of parental overprotection was less than the effect of parental care. This study implies that parental care attitude and ego-resilience play important roles in the continuous development of adolescents' self-regulated learning ability.

Changes of Texture and Plastic Strain Ratio of Asymmetrically Rolled and Annealed Cu Sheet (II) (비대칭 압연과 열처리한 Cu 판의 집합조직과 소성변형비 변화 (II))

  • Lee, C.W.;Jeong, J.H.;Lee, D.N.;Kim, I.
    • Transactions of Materials Processing
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    • v.29 no.2
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    • pp.69-75
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    • 2020
  • The plastic strain ratio is one of the factors that affect the deep drawability of metal sheets. The plastic strain ratio of fully annealed Cu sheet is low, due to its texture being {001}<100>. In this study, in order to increase the plastic strain ratio of Cu sheets we investigated the effect of two treatments: 1st the sheet was asymmetrically rolled and annealed, and 2nd the sheet was symmetrically and asymmetrically rolled and subsequently annealed. The average plastic strain ratio (Rm) of the initial Cu sheet was 0.95 and |Δr| was 1.27. After the 2nd treatment of 5.3% symmetric rolling and annealing of Cu sheet at 1000℃ for 60 min in Ar gas condition, the Rm was 2.29 times higher and the |Δr| was 1.44 times higher than that of initial Cu sheet specimen. After the 2nd treatment of 8.2% asymmetric rolling and annealing of Cu sheet at 1000℃ for 60 min in Ar gas conditions, the Rm was 2.51 times higher and |Δr| was 0.53 times lower than that of the initial Cu sheet specimen. These results can be attributed to the change in texture of the Cu sheets due to the differences in the two methods of rolling.

Oral Health Education Status of Elementary School in Gangwon-Do (강원도 초등학교 구강보건교육 실태)

  • Ryu, Da-Young;Ma, Deuk-Sang
    • Journal of the Korean Society of School Health
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    • v.21 no.1
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    • pp.33-41
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    • 2008
  • Purpose : The purpose of this study was to provide the basic data for p lanning oral health education in elementary schol. Methods conducted using a postal, self-administered questionnaire. A questionnaire containing 19 questions about oral health education which was provided by health teachers in elementary schol was used for data colle ction. Total response rate was 64.3% (119 out of 185). Results : Oral health education except one through textbooks was conduc of which taught oral health themselves. The contents of oral he alth education through special clas was mainly focusing on the tothbrushing method, dental caries, and the use of fluo ride. Acording to the grades clasified by the level of education, the order was the 3rd, 1st, and 2nd grade, which mea ns that the lower grades got more training than the h was acquired through the internet (72.2%), health-related organizations or academies (51.5%). Also, materi als for oral health education were obtained from health-related organizations or academies (67%), self-productio n (49.5%). According to the data during the past 5 years it was found that 13.4% health teachers had oral health-related that more traing neded to be conducted from 89.7% health tea chers. The most dificult problem in oral health education were insufficient time (56.7%). Conclusion : For effective oral health education, there needs a developmen t of targeted goal to achieve a systemic oral health education for each grades as wel as a ned for as health teachers to eficiently acquire knowledge and materials for oral health education, it is necessary to hold regular workshops for health teachers, and develop and distribute appro priate educational materials.

A Study of the Correlation between Academic Achievement and Personality test in a Nursing College (간호학생의 학업성적과 성격 검사와의 상관연구)

  • 이숙자
    • Journal of Korean Academy of Nursing
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    • v.7 no.2
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    • pp.95-106
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    • 1977
  • I. Purpose of the study An ultimate objectives of entrance examination for the selection of students by the nursing school is to evaluate their abilities and readiness for the further study as a competent and professional nurse at the college. As a method to measure the above mentioned achievement Inchon Nursing College conducts personality test in addition to the college made entrance examination. The study amid to find out the correlation between personality test and academic achievement including clinic nursing practice through analysing the data and affecting factors for the purpose of improvement and desirable entrance examination system. II. Content and Method of the study Selected experimental groups were 36 students who were admitted to the college in 1973 and 35 senior students who enrolled in the second semester in 1976. Gathered date, personality test achievement academic achievement record in the college and achievement of clinic nursing practice, from the total number of 71 students were analysed for the clarification of their correlation. III. Findings of the study 1. It was revealed that the correlation between the entrance examination achievement and personality test was meaningful. 2. Found no significant correlation between personality test and academic achievement through 3 years. 3. It was revealed the meaningful correl, n between personality test and clinic nursing practice. 4. The most dominant affecting elements for the academic achievement was the 2 nd year academes achievement showing 91.39%. Approximately 8% were affecting for the 1 st year and 2 nd year academic achievement by the entrance examination achievement and personality test. 5. Achievement of clinic nursing practice was greatly affected by the 2 nd year clinic nursing practice with 81.26%. Achievement of the 3 rd year clinic nursing practice and personality test were affecting with approximately 15%.

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A comparison of fixation methods using three-dimensional finite element analysis following anterior segmental osteotomy

  • Yun, Kyoung In;Park, Min-Kyu;Park, Myung-Kyun;Park, Je Uk
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.38 no.6
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    • pp.332-336
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    • 2012
  • Objectives: This study sought to evaluate fixation methods and determine the best method for the postoperative stabilization of maxillary osteotomy. For our analysis we performed a three-dimensional finite element analysis of stress distribution on the plate, screw, and surrounding bone, as well as displacement onto the plate. Materials and Methods: We generated a model using synthetic skull scan data; an initital surface model was changed to a solid model using software. Modified anterior segmental osteotomy (using Park's method) was made using the program, and four different types of fixation methods were used. An anterior load of 100 N was applied on the palatal surface of two central incisors. Results: The Type 1 (L-shaped) fixation method gave stresses of 187.8 MPa at the plate, 45.8 MPa at the screw, and 15.4 MPa at the bone around the plate. The Type 2 (I-shaped) fixation method gave stresses of 186.6 MPa at the plate, 75.7 MPa at the screw, and 13.8 MPa at the bone around the plate. The Type 3 (inverted L-shaped) fixation method gave stresses of 28.6 MPa at the plate, 29.9 MPa at the screw, and 15.3 MPa at the bone around the plate. The Type 4 (I-shaped) fixation method gave stresses of 34.8 MPa at the plate, 36.9 MPa at the screw, and 14.9 MPa at the bone around the plate. The deflection of the plates for the four fixation methods was 0.014 mm, 0.022 mm, 0.017 mm, and 0.018 mm, respectively. Conclusion: The Type 3 (inverted L-shaped) fixation method offers more stability than the other fixation methods. We therefore recommend this method for the postoperative stabilization of maxillary osteotomy.

The Research Trends on the Acupuncture Treatment of Lumbar Herniated Intervertebral Disc Using PubMed Database (PubMed 검색을 통한 요추 추간판 탈출증의 침치료 연구 동향)

  • Shin, Woo-Suk;Park, Won-Hyung;Cha, Yun-Yeop
    • Journal of Korean Medicine Rehabilitation
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    • v.24 no.4
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    • pp.49-60
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    • 2014
  • Objectives The purpose of this study is to research current trends of acupuncture treatment of lumbar herniated intervertebral disc using the PubMed database. Methods We set up the search strategy and investigated clinical trials on acupuncture treatment of lumbar herniated intervertebral disc through PubMed search. This study analyzed previous researched papers published from January 1st, 2000 to April 30th, 2014, and classified them by publication year, journal names, types of literature, treatment methods and evaluation scales. To assess the quality of the reviewed literature, randomized controlled trial (RCT) studies were assessed by Cochrane's risk of bias (ROB) tool and non-RCT studies were assessed by risk of bias for non-randomized studies (RoBANS). Results We found 35 studies on the acupuncture treatment of lumbar herniated intervertebral disc. Papers on this topic have been published, on average, three to four times annually in 9 journals since the mid-2000's. The journal with the largest number of publications was Chinese Acupuncture & Moxibustion, and most of articles were classified as RCT. Acupuncture treatment was performed individually or together with other treatments. The most frequently used pain evaluation index was visual analogue scale (VAS). The index of effective rate was used frequently but there was a lack of objectivity. In regards to the quality of the studies, outcome assessment in RCT showed that random sequence generation, allocation concealment, and the blinding of participants and personnel increase potential of risk of bias. For non-RCT assessment, outcome showed that confounding variable, measurement of intervention were at high risk of bias. Conclusions In order to obtain objective clinical evidence of acupuncture treatment of lumbar herniated intervertebral disc, further clinical studies should be designed to minimize the risk of bias, using STRICTA with larger sample sizes.

Pattern of Pharmacist Consultation among the Health Counseling Services via Internet Portal Sites: A Pilot Study (국내 포털사이트에서의 지식검색서비스를 이용한 약사와의 상담 패턴에 대한 시험적 연구)

  • Kim, Heejin;Park, Jun Ha;Ji, Eunhee
    • Korean Journal of Clinical Pharmacy
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    • v.26 no.4
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    • pp.324-329
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    • 2016
  • Background: With the development of information and communication technology, there is a growing number of people looking for health information on the internet. This pilot study was performed to analyze the pattern of pharmacist consultation on the internet portal site. Methods: Questions and answers posted on the portal site "Naver Jisik-iN" from March 1st to 31th in 2016 were collected. Medications asked were categorized into prescription drugs, nonprescription drugs, sanitary aids, emergency drugs, functional health foods, and others. Medications were subcategorized into 14 according to the anatomical therapeutic chemical classification system. Questions were divided into 10 categories based on Hepler's drug-related problems. Results: Of the 955 cases, females accounted for 59.5% and inquirers from 11 to 40 years old, 89.4%. The number of prescription drugs, nonprescription drugs, sanitary aids, emergency drugs, functional health foods, and others were 428 (44.8%), 328 (34.3%), 31 (3.3%), 2 (0.2%), 122 (12.8%), and 44 (4.6%), respectively. Questions for drugs acting on alimentary tract and metabolism took up 27.4% followed by those on nervous system, 13.6% and anti-infectives for systemic use, 12.2%. Pharmacist consultation regarding drug information, drug interaction, and adverse reaction occupied 47.9%, 15.2%, and 12.3%, respectively. Conclusion: Health counseling through online is predicted to increase steadily, so pharmacists should broaden their boundaries beyond off-line pharmacies to meet social needs.