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Alteration of Immunoreactivity for SNARE Proteins in the Rat Hippocampus after Middle Cerebral Artery Occlusion  

Park, Jung-Sun (Department of Pharmacology and Medical Research Institute, College of Medicine, Ewha Womans University)
Huh, Pil-Woo (Department of Neurosurgery, The Catholic University of Korea, Uijeongbu St. Mary's Hospital)
Jung, Yeon-Joo (Department of Pharmacology and Medical Research Institute, College of Medicine, Ewha Womans University)
Park, Su-Jin (Department of Pharmacology and Medical Research Institute, College of Medicine, Ewha Womans University)
Lee, Kyung-Eun (Department of Pharmacology and Medical Research Institute, College of Medicine, Ewha Womans University)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.8, no.3, 2004 , pp. 141-146 More about this Journal
Abstract
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) proteins, composed of two presynaptic membrane proteins [synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin] and a presynaptic vesicular protein [vesicle-associated membrane protein (VAMP)], serve as a core of exocytotic fusion machinery, which can be affected by ischemia. Synaptic protein in core region, striatum and cortex has been shown to alter after focal ischemia, however, little is known in hippocampus. Hippocampus is remote from ischemic core, but it is one of the most vulnerable regions. Using immunohistochemistry, the present study was undertaken to investigate the alteration of expression of SNAP-25, syntaxin, and VAMP in the hippocampus of rats which were subjected to middle cerebral artery occlusion (MCAO) for 2h and allowed to reperfuse. At 2 weeks of reperfusion, the SNAP-25 and syntaxin immunoreactivity was increased in the stratum oriens of the CA1 and the stratum lucidum of the CA3 in the ipsilateral hippocampus. However, VAMP immunoreactivity didn't show significant change. These results demonstrate that the level of the presynatpic plasma membrane proteins (SNAP-25 and syntaxin) in the rat hippocampus is more sensitively affected by focal ischemia than that of the synaptic vesicle protein (VAMP).
Keywords
Middle cerebral artery occlusion; Hippocampus; SNAP25; Syntaxin; VAMP;
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