• Computers and Concrete
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• 제24권3호
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• pp.237-247
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• 2019

The key issue for the finite element analysis (FEA) of section steel reinforced concrete (SRC) structure is how to consider the bond-slip performance. However, the bond-slip performance is hardly considered in the FEA of SRC structures because it is difficult to achieve in the finite element (FE) model. To this end, the software developed by Python can automatically add spring elements for the FE model in ABAQUS to considering bond-slip performance. The FE models of the push-out test were conducted by the software and calculated by ABAQUS. Comparing the calculated results with the experimental ones showed that: (1) the FE model of SRC structure with the bond-slip performance can be efficiently and accurately conducted by the software. For the specimen with a length of 1140 mm, 3565 spring elements were added to the FE model in just 6.46s. In addition, different bond-slip performance can also be set on the outer side, the inner side of the flange and the web. (2) The results of the FE analysis were verified against the corresponding experimental results in terms of the law of the occurrence and development of concrete cracks, the stress distribution on steel, concrete and steel bar, and the P-S curve of the loading and free end.

• Advances in concrete construction
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• 제8권3호
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• pp.173-185
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• 2019

This article presents a comparative study of the effect of steel layouts on the seismic behavior of transition steel-concrete composite connections, both experimental and analytical investigations of concrete filled steel tube-reinforced concrete (CFST-RC) and steel reinforecd concrete-reinforced concrete (SRC-RC) structures were conducted. The steel-concrete composite connections were subjected to combined constant axial load and lateral cyclic displacements. Tests were carried out on four full-scale connections extracted from a real project engineering with different levels of axial force. The effect of steel layouts on the mechanical behavior of the transition connections was evaluated by failure modes, hysteretic behavior, backbone curves, displacement ductility, energy dissipation capacity and stiffness degradation. Test results showed that different steel layouts led to significantly different failure modes. For CFST-RC transition specimens, the circular cracks of the concrete at the RC column base was followed by steel yielding at the bottom of the CFST column. While uncoordinated deformation could be observed between SRC and RC columns in SRC-RC transition specimens, the crushing and peeling damage of unconfined concrete at the SRC column base was more serious. The existences of I-shape steel and steel tube avoided the pinching phenomenon on the hysteresis curve, which was different from the hysteresis curve of the general reinforced concrete column. The hysteresis loops were spindle-shaped, indicating excellent seismic performance for these transition composite connections. The average values of equivalent viscous damping coefficients of the four specimens are 0.123, 0.186 and 0.304 corresponding to the yielding point, peak point and ultimate point, respectively. Those values demonstrate that the transition steel-concrete composite connections have great energy dissipating capacity. Based on the experimental research, a high-fidelity ABAQUS model was established to further study the influence of concrete strength, steel grade and longitudinal reinforcement ratio on the mechanical behavior of transition composite connections.

• 한국강구조학회 논문집
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• 제15권3호
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• pp.251-259
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• 2003

본 논문은 매립형 SRC기둥의 축력과 변형능력과의 관계를 찾아내기 위하여 해석적인 연구를 수행하였다. 해석 모델은 캔틸레버기둥으로 한정하였으며 SRC 기둥의 비탄성 거동에 영향을 미치는 변수들인 철골비, 매립철골형상과 전단스팬과 춤과의 비에 대하여 검토하였다. 일정회전각하에 축력과 반복수평력을 받는 SRC기둥의 안정적 거동을 보장하기 위한 최대한계축력이 있다는 것을 해석결과들은 보여 주고 있다. 반복 횡하중을 받는 기둥이 저항할 수 있는 최대축력은 소요회전각을 보장하는 안정한계축력으로서 정의된다. 해석결과에 따르면 안정한 계축력비는 강재의 강도가 증가함에 따라 콘크리트의 강도가 감소함에 따라 증가하는 것으로 나타났다. 또한 I형 단면이 매립형 철골로 사용될때 철골 단면적이 증가함에 따라 안정한계 축력비는 증가하며, 십자형 단면이 사용되는 경우에는 단면적에 의한 영향이 거의 없었다.

• 생약학회지
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• 제45권2호
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• pp.127-134
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• 2014

A great interest is emerging about green tea as a tool against human cancer proliferation or inflammation, as pointed out by recent reports describing the inhibitory action of epigallocatechin gallate (EGCG) on angiogenesis, urokinase, metalloproteinases, and induction of inducible nitric oxide synthase. We proposed that EGCG may regulate a multi target signaling having wider spectra of action than those actions of single enzymes. CSK (c-terminal Src kinase) protein is a non-receptor tyrosine kinase involved in the cross-talk and mediation of many signaling pathways that promote cell proliferation, adhesion, invasion, migration, and tumorigenesis. Based on the knowledge that CSK activation is important for cancer proliferation we hypothesized that CSK could be a target of EGCG. Here we showed that EGCG effectively suppressed the growth of CSK MEF cell when compare with CSK knockout MEF cell growth. These results indicate that EGCG could be used as a chemoprevention to modulate CSK signal pathway in inflammatory processes and tumor formation.

• 전기학회논문지
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• 제61권3호
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• pp.394-400
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• 2012

In this paper, charging modes of series resonant converter for a high voltage energy storage capacitor are compared in terms of charging time, peak resonant current, normalized peak resonant current and voltage in each operation mode. Operating principles of the full bridge series resonant converter with capacitor load are explained and analyzed in discontinuous and continuous operation mode. Based on the analysis and simulation result, $0.6{\omega}_r$ < ${\omega}_s$ < $0.75{\omega}_r$ and $1.3{\omega}_r$ < ${\omega}_s$ < $1.4{\omega}_r$ are evaluated to the best range of switching frequency for charging of an high voltage energy storage capacitor. 1.8 kJ/s SRC prototype is assembled with TI 28335 DSP controller and 40 kJ, 7 kV energy storage capacitor. Design rules based on the comparative analysis are verified by experiment.

• BMB Reports
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• 제30권5호
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• pp.303-307
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• 1997

Grb2, which is composed of a Src homology 2 (SH2) domain and two Src homology 3 (SH3) domains, is known to serve as an adaptor protein in signaling for Ras activation. Thus, a blocker of the Grb2 interactions with other proteins can be a potential candidate for an anticancer drug. In this study, we have developed a high throughput screening method for SH3 domain binding ligands and blockers. Firstly, we made and purified the glutathione S-transferase (GST)-fusion proteins with the Grb2 SH2 and SH3 domains, and the entire Grb2. This method measures the binding of a biotin-labeled oligopeptide, derived from a Grb2/SH3 binding motif in the hSos, to the GST-fusion proteins, which are precoated as glutathione S-transferase fusion protein on a solid phase. When $1\;{\mu}g$ of each fusion protein was used to coat the wells, both N- and C- terminal SH3 the domains as well as the whole of Grb2 were able to interact with the biotin-conjugated ligand peptide, while the SH2 domain and GST alone showed no binding affinity. Although N- and C- terminal SH3 domains showed an increase of binding to the ligand peptide in proportion to the amount of peptide, the GST fusion protein with Grb2 demonstrated much higher binding affinity. GST-Grb2 coating on the solid phase showed a saturation curve; 66 and 84% of the maximal binding was observed at 100 and 300 ng/$100\;{\mu}l$, respectively. This binding assay system was peptide sequence-specific, showing a dose-dependent inhibition with the unlabeled peptide of SH3 binding motif. Several other peptides, such as SH2 domain binding motifs and PTB domain binding motif, were ineffective to inhibit the binding to the biotin-conjugated ligand peptide. These results suggest that our method may be useful to screen for new anticancer drug candidates which can block the signaling pathways mediated by SH3 domain binding.

• 한국강구조학회 논문집
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• 제24권5호
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• pp.535-547
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• 2012

PSRC 기둥은 앵글을 콘크리트에 매입시킨 기둥으로, 단면의 외곽 코너에 배치되는 앵글이 기둥의 휨-압축에 저항하고, 횡철근은 기둥의 전단과 앵글-콘크리트 사이의 부착에 저항한다. 본 연구에서는 KBC 2009에 따라 PSRC 합성기둥의 휨, 전단, 부착 설계방법을 정립하고, 단순지지된 2/3 스케일의 PSRC 보와 SRC 보의 2점 가력 휨실험을 통하여 제안된 설계법을 검증하고 PSRC 합성기둥의 파괴특성을 분석하였다. 단면의 강재비와 횡철근 간격을 실험 변수로 고려하였다. 실험결과, KBC 2009으로 예측한 PSRC 합성기둥의 휨, 전단, 부착 강도는 실험결과와 잘 일치하였다. 고강도 앵글이 기둥 단면의 외곽에 배치되므로 PSRC 합성기둥은 동일한 강재비를 갖는 일반 SRC 합성기둥 단면에 비하여 매우 우수한 휨저항 성능을 나타냈다. 그러나 앵글과 콘크리트 사이의 부착강도가 충분히 학보되지 못한 경우 합성기둥 단면의 휨항복강도를 발휘하기 이전에 앵글의 부착파괴, 피복콘크리트 파괴, 횡철근의 파단 등이 발생하였다. 또한 앵글 용접성 및 인성이 부족할 경우 앵글-횡철근 용접부에서 앵글의 파단에 의해 실험체가 파괴되었다.

• 한국구조물진단유지관리공학회 논문집
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• 제18권3호
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• pp.40-48
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• 2014

본 연구에서는 기존 철근콘크리트 건물의 보-기둥 접합부의 내진성능의 개선을 위해 보-기둥 접합부 영역을 FRP보강재(매입형 CFRP Rod, CFRP시트)를 사용하여 보강한 후 내진성능을 평가하였다. 총 6개의 실험체를 제작하고 실험을 수행하여 내진성능을 평가하였으며, 본 연구의 실험결과를 근거로 다음과 같은 결론을 얻었다. 기존 철근콘크리트 보-기둥 접합부의 접합부 영역을 보강한 결과, 초기 재하시 접합부 영역의 균열억제 효과와 재하 전 과정을 통하여 보강재의 구속효과로 인하여 균열억제 효과가 커서 안정적인 파괴형태 및 내력향상 효과를 나타내었다. 매입형 CFRP Rod와 CFRP시트를 활용한 RC 외부 보-기둥 접합부 실험체 RBCJ-SRC2는 표준실험체 RBCJ와 비교하여 변위연성4, 7에서 각각 최대 내력은 1.97배, 에너지소산능력은 2.08배 증가하였다. 또한, 실험체 RBCJ-SR시리즈와 비교하여 최대내력이 1.09~1.11배 증가하였다. 그리고 실험체 RBCJ-CS, RBCJ-SR시리즈, RBCJ-SRC2는 변위연성 5, 6에서 표준실험체 RBCJ 보다 에너지소산능력이 1.10~2.30배 증가하였다. 그리고 에너지소산능력은 변위연성 4에서 13.0~14.4% 증가하였다.

• 한국안광학회지
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• 제21권1호
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• pp.69-76
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• 2016

목적: 본 연구는 망막색소상피층에 색소가 존재하는 mouse에서 청색광으로 인해 광수용세포 손상이 일어날 수 있는지 확인하고, 광수용세포 중 특이적 세포에서 세포사멸이 유도되는지 조사하여 청색광에 의해 야기될 수 있는 연령관련 황반변성의 기전 규명과 치료제 개발에 도움이 되고자 진행되었다. 방법: C57black mice를 24시간 암순응 시켜 463 nm의 청색광을 $2800{\pm}10lux$로 조사한 후 1일, 3일, 7일째에 안구를 적출하였다. 청색광의 자극은 GFAP(Glial fibrillary acidic protein)단백질의 발현을 이용하여 확인하였고, 광수용세포의 세포사멸은 TUNEL(Terminal deoxynucleotidyl transferase dUTP nick end labeling)을 사용하여 분석하였다. Western blotting으로 ERK(Extracellular signal-regulated kinases), c-JUN, SRC(Sarcoma) 단백질 발현을 확인하였고, 막대세포와 원뿔세포의 손상 정도를 비교하기 위해 면역염색으로 분석하였다. 결과: 청색광을 조사한 후 1, 3, 7일이 지난 망막은 대조군 보다 전체적으로 두께가 감소하였고, 각 얼기층보다 핵층에서 두께 감소를 확인할 수 있었다. 또한 청색광을 조사한 후 1일 지난 Muller glia에서 GFAP 단백질이 증가하는 것을 확인하였다. TUNEL 염색에서는 청색광을 조사한 후 1일 지난 망막의 광수용세포에서 가장 많은 발현을 보였다. 세포사멸 기전 과정 중 하나임을 확인하기 위해 ERK, c-JUN, SRC 단백질 활성을 확인한 결과 청색광을 조사한 망막에서 phosphorylated ERK는 증가하였고 phosphorylated SRC는 조사 후 1일에서만 증가를 나타내었으며, 반대로 phosphorylated c-JUN은 조사 후 1일에서만 감소하였다. 청색광을 조사한 망막에서 막대세포 발색단인 로돕신과 원뿔세포의 발색단인 옵신이 감소하였으며, 옵신의 감소량은 로돕신의 감소량보다 큰 것을 확인하였다. 결론: 본 연구는 청색광이 망막에 손상자극을 주고, ERK와 SRC 신호전달과 관련하여 광수용세포의 세포사멸을 일으킬 수 있으며 청색광이 광수용세포 중 원뿔세포의 세포사멸을 직접적으로 유도하여 망막 손상을 야기할 수 있다는 가능성을 제시하였다.

• Genomics & Informatics
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• 제9권3호
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• pp.102-113
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• 2011

C-terminal SRC kinase (CSK) is a ubiquitously expressed, cytosolic enzyme that phosphorylates and inactivates several SRC family protein tyrosine kinases. Recent genomewide association studies have implicated CSK in the regulation of blood pressure. The current study aim is to determine the blood pressure association of the genes regulated by CSK down-regulation. The CSK mRNA expression was downregulated in vascular smooth muscle cells using small interfering RNA (siRNA). CSK mRNA levels fell by 90% in cells that were treated with CSK siRNA; the RNA from these cells was examined by microarray using the Illumina HumanRef-8 v3 platform, which comprises 24,526 reference mRNA probes. On treatment with CSK siRNA, 19 genes were downregulated by more than 2-fold and 13 genes were upregulated by more than 2-fold. Three (CANX, SLC30A7, and HMOX1) of them revealed more than 3 fold differential expression. Interestingly, the HMOX1 SNPs were associated with diastolic blood pressure in the 7551 Koreans using Korea Association REsource data, and the result was supported by the other reports that HMOX1 linked to blood vessel maintenance. Among the remaining 29 differentially expressed genes, seven (SSBP1, CDH2, YWHAE, ME2, PFTK1, G3BP2, and TUFT1) revealed association with both systolic and diastolic blood pressures. The CDH2 gene was linked to blood pressures. Conclusively, we identified 32 differentially expressed genes which were regulated by CSK reduction, and two (HOMX1 and CDH2) of them might influence the blood pressure regulation through CSK pathway.