• Earthquakes and Structures
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• v.14 no.4
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• pp.337-347
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• 2018

This paper presents some shaking table tests conducted on a 1/4-scaled model with 5-story steel reinforced concrete (SRC) spatial frame with irregular section columns under a series of base excitations with gradually increasing acceleration peaks. The test frame was subjected to a sequence of seismic simulation tests including 10 white noise vibrations and 51 seismic simulations. Each seismic simulation was associated with a different level of seismic disaster. Dynamic characteristic, strain response, acceleration response, displacement response, base shear and hysteretic behavior were analyzed. The test results demonstrate that at the end of the loading process, the failure mechanism of SRC frame with irregular section columns is the beam-hinged failure mechanism, which satisfies the seismic code of "strong column-weak beam". With the increase of acceleration peaks, accumulated damage of the frame increases gradually, which induces that the intrinsic frequency decreases whereas the damping ratio increases, and the peaks of acceleration and displacement occur later. During the loading process, torsion deformation appears and the base shear grows fast firstly and then slowly. The hysteretic curves are symmetric and plump, which shows a good capacity of energy dissipation. In summary, SRC frame with irregular section columns can satisfy the seismic requirements of "no collapse under seldom earthquake", which indicates that this structural system is suitable for the construction in the high seismic intensity zone.

• Journal of Korean Society of Steel Construction
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• v.15 no.3
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• pp.251-259
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• 2003

In this paper, an analytical approach hwas been conductsed to clarify the relationships between the axial force and the deformation capacity of steel- encased reinforced- concrete beam-columns. The analytical model was defined as a cantilever. Several parameters influencing the inelastic performance of the beam-columns were selected, as follows: including encased steel area ratios, and sectional shapes of the encased steel, material strengths, and shear-span- to-depth ratios. The Analytical results of the analysis showed that the axial force had to have a maximum limit to ensure the stable behavior of a steel- encased reinforced- concrete beam-column when it was subjected to both axial and repeated lateral loading under a constant rotation angle amplitude. The maximum axial force of the beam-column to be resisted under cyclic lateral loading was defined as the stable-limit axial force to ensure the required rotation angle amplitude. The Analytical results of the analysis indicate that the stable-limit axial load ratio increases as the steel strength increases or as the compressive strength of the concrete decreases. The stable-limit axial load ratio decreases as the encased steel ' s sectional area increases in the case of a 1-shaped sections and it is almost not influenced by the steel sectional area in the case of a cross-shaped section.

• Structural Engineering and Mechanics
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• v.76 no.4
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• pp.465-477
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• 2020

This paper presents an investigation on the dynamic behavior of SRC columns with built-in cross-shaped steels under impact load. Seven 1/2 scaled SRC specimens were subjected to low-speed impact by a gravity drop hammer test system. Three main parameters, including the lateral impact height, the axial compression ratios and the stirrup spacing, were considered in the response analysis of the specimens. The failure mode, deformation, the absorbed energy of columns, as well as impact loads are discussed. The results are mainly characterized by bending-shear failure, meanwhile specimens can maintain an acceptable integrity. More than 33% of the input impact energy is dissipated, which demonstrates its excellent impact resistance. As the impact height increases, the flexural cracks and shear cracks observed on the surface of specimens were denser and wider. The recorded time-history of impact force and mid-span displacement confirmed the three stages of relative movement between the hammer and the column. Additionally, the displacements had a notable delay compared to the rapid changes observed in the measured impact load. The deflection of the mid-span did not exceed 5.90mm while the impact load reached peak value. The impact resistance of the specimen can be improved by proper design for stirrup ratios and increasing the axial load. However, the cracking and spalling of the concrete cover at the impact point was obvious with the increasing in stiffness.

• Experimental and Molecular Medicine
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• v.50 no.12
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• pp.11.1-11.9
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• 2018

Estrogen has diverse effects on cardiovascular function, including regulation of the contractile response to vasoactive substances such as serotonin. The serotonin system recently emerged as an important player in the regulation of vascular tone in humans. However, hyperreactivity to serotonin appears to be a critical factor for the pathophysiology of hypertension. In this study, we examined the modulatory mechanisms of estrogen in serotonin-induced vasoconstriction by using a combinatory approach of isometric tension measurements, molecular biology, and patch-clamp techniques. $17{\beta}$-Estradiol (E2) elicited a significant and concentration-dependent relaxation of serotonin-induced contraction in deendothelialized aortic strips isolated from male rats. E2 triggered a relaxation of serotonin-induced contraction even in the presence of tamoxifen, an estrogen receptor antagonist, suggesting that E2-induced changes are not mediated by estrogen receptor. Patch-clamp studies in rat arterial myocytes showed that E2 prevented Kv channel inhibition induced by serotonin. Serotonin increased Src activation in arterial smooth muscle required for contraction, which was significantly inhibited by E2. The estrogen receptor-independent inhibition of Src by E2 was confirmed in HEK293T cells that do not express estrogen receptor. Taken together, these results suggest that estrogen exerts vasodilatory effects on serotonin-precontracted arteries via Src, implying a critical role for estrogen in the prevention of vascular hyperreactivity to serotonin.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.4
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• pp.449-456
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• 2017

Beauvericin (BEA), a cyclic hexadepsipeptide produced by the fungus Beauveria bassiana, is known to have anti-cancer, anti-inflammatory, and anti-microbial actions. However, how BEA suppresses macrophage-induced inflammatory responses has not been fully elucidated. In this study, we explored the anti-inflammatory properties of BEA and the underlying molecular mechanisms using lipopolysaccharide (LPS)-treated macrophage-like RAW264.7 cells. Levels of nitric oxide (NO), mRNA levels of transcription factors and the inflammatory genes inducible NO synthase (iNOS) and interleukin (IL)-1, and protein levels of activated intracellular signaling molecules were determined by Griess assay, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), luciferase reporter gene assay, and immunoblotting analysis. BEA dose-dependently blocked the production of NO in LPS-treated RAW264.7 cells without inducing cell cytotoxicity. BEA also prevented LPS-triggered morphological changes. This compound significantly inhibited nuclear translocation of the $NF-{\kappa}B$ subunits p65 and p50. Luciferase reporter gene assays demonstrated that BEA suppresses MyD88-dependent NF-${\kappa}B$ activation. By analyzing upstream signaling events for $NF-{\kappa}B$ activation and overexpressing Src and Syk, these two enzymes were revealed to be targets of BEA. Together, these results suggest that BEA suppresses $NF-{\kappa}B$-dependent inflammatory responses by suppressing both Src and Syk.

• Steel and Composite Structures
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• v.16 no.3
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• pp.253-267
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• 2014

Based on the test on a 1/2.5-scaled model of a two-bay and three-story inner frame composed of reinforced concrete beams and lattice steel reinforced concrete (SRC) irregular section columns under low cyclic reversed loading, the failure process and the features of the frame were observed. The subsequence of plastic hinges of the structure, the load-displacement hysteresis loops and the skeleton curve, load bearing capacity, inter-story drift ratio, ductility, energy dissipation and stiffness degradation were analyzed. The results show that the lattice SRC inner frame is a typical strong column-weak beam structure. The hysteresis loops are spindle-shaped, and the stiffness degradation is insignificant. The elastic-plastic inter-story deformation capacity is high. Compared with the reinforced concrete frame with irregular section columns, the ductility and energy dissipation of the structure are better. The conclusions can be referred to for seismic design of this new kind of structure.

• Computers and Concrete
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• v.24 no.3
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• pp.237-247
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• 2019

The key issue for the finite element analysis (FEA) of section steel reinforced concrete (SRC) structure is how to consider the bond-slip performance. However, the bond-slip performance is hardly considered in the FEA of SRC structures because it is difficult to achieve in the finite element (FE) model. To this end, the software developed by Python can automatically add spring elements for the FE model in ABAQUS to considering bond-slip performance. The FE models of the push-out test were conducted by the software and calculated by ABAQUS. Comparing the calculated results with the experimental ones showed that: (1) the FE model of SRC structure with the bond-slip performance can be efficiently and accurately conducted by the software. For the specimen with a length of 1140 mm, 3565 spring elements were added to the FE model in just 6.46s. In addition, different bond-slip performance can also be set on the outer side, the inner side of the flange and the web. (2) The results of the FE analysis were verified against the corresponding experimental results in terms of the law of the occurrence and development of concrete cracks, the stress distribution on steel, concrete and steel bar, and the P-S curve of the loading and free end.

• Earthquakes and Structures
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• v.20 no.4
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• pp.417-430
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• 2021

Due to functional requirements, SRC column-RC beam abnormal joints with characteristics of strong beam weak column, variable column section, unequal beam height and staggered height exist in the Steel reinforced concrete (SRC) frame-bent main building structure of thermal power plant (TPP). This paper presents the experimental results of these abnormal joints through cyclic loading tests on five specimens with scaling factor of 1/5. The staggered height and whether adding H-shaped steel in beam or not were changing parameters of specimens. The failure patterns, bearing capacity, energy dissipation and ductile performance were analyzed. In addition, the stress mechanism of the abnormal joint was discussed based on the diagonal strut model. The research results showed that the abnormal exterior joints occurred shear failure and column end hinge flexural failure; reducing beam height through adding H-shaped steel in the beam of abnormal exterior joint could improve the crack resistance and ductility; the abnormal interior joints with different staggered heights occurred column ends flexural failure; the joint with larger staggered height had the higher bearing capacity and stiffness, but lower ductility. The concrete compression strut mechanism is still applicable to the abnormal joints in TPP, but it is affected by the abnormal characteristics.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.3
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• pp.394-400
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• 2012

In this paper, charging modes of series resonant converter for a high voltage energy storage capacitor are compared in terms of charging time, peak resonant current, normalized peak resonant current and voltage in each operation mode. Operating principles of the full bridge series resonant converter with capacitor load are explained and analyzed in discontinuous and continuous operation mode. Based on the analysis and simulation result, $0.6{\omega}_r$ < ${\omega}_s$ < $0.75{\omega}_r$ and $1.3{\omega}_r$ < ${\omega}_s$ < $1.4{\omega}_r$ are evaluated to the best range of switching frequency for charging of an high voltage energy storage capacitor. 1.8 kJ/s SRC prototype is assembled with TI 28335 DSP controller and 40 kJ, 7 kV energy storage capacitor. Design rules based on the comparative analysis are verified by experiment.

• Genomics & Informatics
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• v.9 no.3
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• pp.102-113
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• 2011

C-terminal SRC kinase (CSK) is a ubiquitously expressed, cytosolic enzyme that phosphorylates and inactivates several SRC family protein tyrosine kinases. Recent genomewide association studies have implicated CSK in the regulation of blood pressure. The current study aim is to determine the blood pressure association of the genes regulated by CSK down-regulation. The CSK mRNA expression was downregulated in vascular smooth muscle cells using small interfering RNA (siRNA). CSK mRNA levels fell by 90% in cells that were treated with CSK siRNA; the RNA from these cells was examined by microarray using the Illumina HumanRef-8 v3 platform, which comprises 24,526 reference mRNA probes. On treatment with CSK siRNA, 19 genes were downregulated by more than 2-fold and 13 genes were upregulated by more than 2-fold. Three (CANX, SLC30A7, and HMOX1) of them revealed more than 3 fold differential expression. Interestingly, the HMOX1 SNPs were associated with diastolic blood pressure in the 7551 Koreans using Korea Association REsource data, and the result was supported by the other reports that HMOX1 linked to blood vessel maintenance. Among the remaining 29 differentially expressed genes, seven (SSBP1, CDH2, YWHAE, ME2, PFTK1, G3BP2, and TUFT1) revealed association with both systolic and diastolic blood pressures. The CDH2 gene was linked to blood pressures. Conclusively, we identified 32 differentially expressed genes which were regulated by CSK reduction, and two (HOMX1 and CDH2) of them might influence the blood pressure regulation through CSK pathway.