• Molecules and Cells
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• 제42권11호
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• pp.810-819
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• 2019

For physiological or pathological understanding of bone disease caused by abnormal behavior of osteoclasts (OCs), functional studies of molecules that regulate the generation and action of OCs are required. In a microarray approach, we found the suppression of tumorigenicity 5 (ST5) gene is upregulated by receptor activator of nuclear $factor-{\kappa}B$ ligand (RANKL), the OC differentiation factor. Although the roles of ST5 in cancer and ${\beta}-cells$ have been reported, the function of ST5 in bone cells has not yet been investigated. Knockdown of ST5 by siRNA reduced OC differentiation from primary precursors. Moreover, ST5 downregulation decreased expression of NFATc1, a key transcription factor for osteoclastogenesis. In contrast, overexpression of ST5 resulted in the opposite phenotype of ST5 knockdown. In immunocytochemistry experiments, the ST5 protein is colocalized with Src in RANKL-committed cells. In addition, ST5 enhanced activation of Src and Syk, a Src substrate, in response to RANKL. ST5 reduction caused a decrease in RANKL-evoked calcium oscillation and inhibited translocation of NFATc1 into the nucleus. Taken together, these findings provide the first evidence of ST5 involvement in positive regulation of osteoclastogenesis via Src/Syk/calcium signaling.

• 한국구조물진단유지관리공학회지
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• 제16권1호
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• pp.19-27
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• 2012

• 콘크리트학회논문집
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• 제19권4호
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• pp.401-409
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• 2007

본 연구는 국내 현장에서 적용되고 있는 SRC 기둥-RC 보 강축 방향 접합부의 구조 성능을 평가하기 위하여 국내 현장 조사 및 기존 연구 자료를 기초로 접합부 상세를 분류하였고, 6개의 실험체를 제작하여 구조 실험을 수행하였다. 실험 결과로부터 하중-변위 이력 및 최대 내력 도달 이후의 내력 저하 등에 대한 거동을 비교 분석하였다. 그 결과 넓은 보 단면 (RCW-P, RCW-W, RCW-F) 및 T형 보 단면 (RCT-W) 실험체들이 H형강 브라켓 부착형 실험체(HBR-L, HBR-S)에 비해 대체로 우수한 구조적 거동을 보여주었으며, 설계 내력을 상회하는 내력을 보유하고 있었다. 또한 현장에서 적용하여도 무리가 없는 것으로 판단된다. 특히, T형 보 단면 (RCT-W)을 갖는 실험체가 최대 내력 및 최대 내력 도달 이후 강도 저하 등 전반적으로 다른 접합 형태를 갖는 실험체에 비해 우수한 구조 성능을 보유하고 있었다. 그리고 H형강 브라켓 부착형 실험체들 (HBR-L, HBR-S)은 설계 내력에 미치지 못한 최대 내력을 보여 주었고 최대 내력 도달 이후 급격한 하중 저하를 나타내며 취성적인 거동을 보였다.

• 한국전산구조공학회논문집
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• 제26권5호
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• pp.335-342
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• 2013

최근 환경오염 문제에 대한 심각성이 대두되며 전 세계적으로 환경부하 저감을 위해 다양한 노력을 쏟고 있다. 특히 환경 저해 산업의 하나인 건설분야에서는 $CO_2$배출량과 에너지 소비량을 줄이기 위해 활발한 연구를 진행해 왔다. 그러나 건설분야의 기존 연구들은 대부분 $CO_2$배출량이 가장 큰 사용 및 유지관리 단계에만 집중하고 있으며, 설계단계에 대한 연구는 2D의 철근콘크리트 부재 및 구조물에 대해서만 실행되었을 정도로 초기단계이다. 사실, LCA적 관점에서 친환경적 건설산업을 이루기 위해서는 건물의 초기설계 단계에서부터 $CO_2$배출량을 저감시키기 위한 방향으로 설계를 유도할 수 있어야 하며, 구조 엔지니어로서 환경성을 고려한 설계안을 제시할 수 있어야 한다. 그러므로 본 연구에서는 매입형 합성기둥(SRC)을 대상으로 $CO_2$최적화 기법을 제시하였으며, 이를 통해 얻은 여러 설계단면을 이용하여 SRC기둥의 $CO_2$배출량에 영향을 미치는 3가지 요소((1) 강재 크기, (2) 콘크리트 압축강도, (3) 작용 하중 크기)에 대한 영향관계를 분석하였다.

• BMB Reports
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• 제48권3호
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• pp.184-189
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• 2015

Src homology 2-containing protein tyrosine phosphatase 2 (SHP-2) is known to protect neurons from neurodegeneration during ischemia/reperfusion injury. We recently reported that ROS-mediated oxidative stress promotes phosphorylation of endogenous SHP-2 in astrocytes and complex formation between caveolin-1 and SHP-2 in response to oxidative stress. To examine the region of SHP-2 participating in complex formation with caveolin-1, we generated three deletion mutant constructs and six point mutation constructs of SHP-2. Compared with wild-type SHP-2, binding of the N-SH2 domain deletion mutant of SHP-2 to p-caveolin-1 was reduced greatly, using flow cytometric competitive binding assays and surface plasmon resonance (SPR). Moreover, deletion of the N-SH2 domain of SHP-2 affected $H_2O_2$-mediated ERK phosphorylation and Src phosphorylation at Tyr 419 in primary astrocytes, suggesting that N-SH2 domain of SHP-2 is responsible for the binding of caveolin-1 and contributes to the regulation of Src phosphorylation and activation following ROS-induced oxidative stress in brain astrocytes.

• Steel and Composite Structures
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• 제26권4호
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• pp.407-420
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• 2018

Experiments were performed to explore the hysteretic performance of steel reinforced concrete (SRC) cross-shaped columns. Nine specimens were designed and tested under the combined action of compression, flexure, shear and torsion. Torsion-bending ratio (i.e., 0, 0.14, 0.21) and steel forms (i.e., Solid - web steel, T - shaped steel, Channel steel) were considered in the test. Both failure processes and modes were obtained during the whole loading procedure. Based on experimental data, seismic indexes, such as bearing capacity, ductility and energy dissipation were investigated in detail. Experimental results suggest that depending on the torsion-bending ratio, failure modes of SRC cross-shaped columns are bending failure, flexure-torsion failure and torsion-shear failure. Shear - displacement hysteretic loops are fuller than torque - twist angle hysteretic curves. SRC cross-shaped columns exhibit good ductility and deformation capacity. In the range of test parameters, the existence of torque does not reduce the shear force but it reduces the displacement and bending energy dissipation capacity. What is more, the bending energy dissipation capacity increases with the rising of displacement level, while the torsion energy dissipation capacity decreases.

• Structural Engineering and Mechanics
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• 제68권2호
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• pp.193-201
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• 2018

This paper describes an experimental study on the seismic performance of steel reinforced concrete (SRC) T-shaped columns. The lateral loads were applied along the web of the column with different loading histories, such as monotonic loading, mixed loading of variable amplitude cyclic loading and monotonic loading, constant amplitude cyclic loading and variable amplitude cyclic loading. The failure modes, load-displacement curves, characteristic loads and displacements, ductility, strength and stiffness degradations and energy dissipation capacity of the column were analyzed. The effects of loading history on the seismic performance were focused on. The test results show that the specimens behaved differently in the aspects of the failure mode subject to different loading history, although all the failure modes can be summarized as flexural failure. The hysteretic loops of specimens are plump, and minimum values of the failure drift angles and ductility coefficients are 1/24 and 4.64, respectively, which reflect good seismic performance of SRC T-shaped column. With the increasing numbers of loading cycles, the column reveals lower bearing capacity and ductility. The strength and stiffness of the column with variable amplitude cyclic loading degrades more rapidly than that with constant amplitude cyclic loading, and the total cumulative dissipated energy of the former is less.

• Steel and Composite Structures
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• 제13권5호
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• pp.473-487
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• 2012

An experimental study was performed to investigate the seismic performance of steel reinforced concrete (SRC) special-shaped columns. For this purpose, 17 steel reinforced concrete special-shaped column specimens under low-cyclic reversed load were tested, load process and failure patterns of the specimens with different steel reinforcement were observed. The test results showed that the failure patterns of these columns include shear-diagonal compression failure, shear-bond failure, shear-flexure failure and flexural failure. The failure mechanisms and characteristics of SRC special-shaped columns were also analyzed. For different SRC special-shaped columns, based on the failure characteristics and mechanism observed from the test, formulas for calculating ultimate shear capacity in shear-diagonal compression failure and shear-bond failure under horizontal axis and oblique load were derived. The calculated results were compared with the test results. Both the theoretical analysis and the experimental results showed that, the shear capacity of T, L shaped columns under oblique load are larger than that under horizontal axis load, whereas the shear capacity of +-shaped columns under oblique load are less than that under horizontal axis load.

• 한국자기공명학회논문지
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• 제14권2호
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• pp.105-116
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• 2010

SH3YL1, a novel protein containing one Src homology 3 domain at the carboxyl terminus was first detected in mouse anagen skin cDNA. This protein had a significant homology with YHRO 16c/Ysc 84, the yeast Src homology 3 domain-containing protein. The sequence identity was remarkable at the carboxyl and amino-terminal Src homology 3 domain, suggesting that the novel protein is a mouse homolog of the yeast protein and thus was termed as SH3YL1. SH3YL1 is composed of two domains, a DUF500 at N-termini and a SH3 domain at C-termini. In our study we cloned the SH3 domain in bacterial expression system in Escherichia coli using pET32a vector with TEV protease cleavage site and purified as a monomer using affinity chromatography. The N-terminal poly-Histidine tag was cleaved with TEV protease and target protein was used for backbone studies. Our study showed that SH3 domain primarily consists of $\beta$-sheet which is in consistence with previous result performed on the truncated SH3 domain of SH3YL1.

• 한국강구조학회 논문집
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• 제26권5호
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• pp.441-452
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• 2014

본 논문에서는 중력하중을 받는 SRC기둥과 합성보 접합부의 정적실험을 수행하였다. 합성보는 H형단면과 U형단면으로 구성되어 있다. 모두 5개의 실대형 실험체를 설계하여 실험변수, 즉, H형단면 크기, 스터드커넥터의 유무, 스티프너와 상부근의 유무 등이 접합부의 거동에 미치는 영향을 조사하였다. 또한 H형단면과 U형단면의 용접접합부의 구조성능을 초기강성, 내력 및 변형능력을 중심으로 기술하였다.