• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2007년도 정기 학술대회 논문집
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• pp.481-486
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• 2007

The Zienkiewicz-Zhu(Z/Z) error estimate is slightly modified for the hierarchical p-refinement, and is then applied to L-shaped plates subjected to bending to demonstrate its effectiveness. An adaptive procedure in finite element analysis is presented by p-refinement of meshes in conjunction with a posteriori error estimator that is based on the superconvergent patch recovery(SPR) technique. The modified Z/Z error estimate p-refinement is different from the conventional approach because the high order shape functions based on integrals of Legendre polynomials are used to interpolate displacements within an element, on the other hand, the same order of basis function based on Pascal's triangle tree is also used to interpolate recovered stresses. The least-square method is used to fit a polynomial to the stresses computed at the sampling points. The strategy of finding a nearly optimal distribution of polynomial degrees on a fixed finite element mesh is discussed such that a particular element has to be refined automatically to obtain an acceptable level of accuracy by increasing p-levels non-uniformly or selectively. It is noted that the error decreases rapidly with an increase in the number of degrees of freedom and the sequences of p-distributions obtained by the proposed error indicator closely follow the optimal trajectory.

• 한국소음진동공학회논문집
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• 제25권8호
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• pp.568-574
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• 2015

The present study was conducted by using stereo pattern recognition method(SPR method) to measure the displacement and vibration of an airplane wing in flight condition. A SPR based measurement system was developed using two visible light stereo cameras. The visible light stereo images were processed to obtain marker points by adaptive threshold method and marker filtering technique. The marker points were used to reconstruct 3D point, displacement, and vibration data. The SPR system was installed on F-16 fighter. The wing displacement and vibration were measured in flight condition. Therefore, this paper presents a possibility that SPR based measurement system using visible light stereo camera can be very useful for measuring displacement and vibration of an airplane in flight condition.

• 한국분무공학회지
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• 제26권4호
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• pp.212-218
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• 2021

The present study aims to develop the Surface Plasmon Resonance (SPR) imaging system facilitating the real-time measurement of the concentration of evaporating binary mixture droplet (BMD). We introduce the theoretical background of the SPR imaging technique and its methodology for concentration measurement. The SPR imaging system established in the present study consists of a LED light source, a polarizer, a lens, and a band pass filter for the collimated light of a 589 nm wavelength, and a CCD camera. Based on the Fresnel multiple-layer reflection theory, SPR imaging can capture the change of refractive index of evaporating BMD. For example, the present study exhibits the visualization process of ethylene glycol (EG)-water (W) BMD and measures real-time concentration change. Since the water component is more volatile than the ethylene glycol component, the refractive index of EG-W BMD varies with its mixture composition during BMD evaporation. We successfully measured the ethylene glycol concentration within the evaporating BMD by using SPR imaging.

• Tuberculosis and Respiratory Diseases
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• 제60권4호
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• pp.412-418
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• 2006

연구배경 : 클라미디아 감염의 진단은 혈청검사로 이루어진다. 현재 표준 방법은 MIF(microimmunofluorescence)이나 이 방법은 주관적이고 시간이 많이 걸리는 단점이 있다. 최근을 SPR(surface plasmon resonance) 센서를 이용한 단백질 칩이 감염의 새로운 진단 방법으로 제시되고 있다. 클라미디아 감염의 진단을 위한 단백질 칩 개발을 위하여 금 칩 표면에 세균을 고정하고 클라미디아 균에 대한 항체와 표면 위 세균과의 반응을 SPR 센서를 이용하여 측정하고자 하였다. 방법 : 표면 항원으로 배양한 Chlamydophila pneumoniae LKK1의 EB를 정제하였다. 양전하를 띤 PDDA (polydiallyldimethylammonium chloride)를 이용하여 전하를 이용한 단백질 칩을 제작하였다. 클라미디아 균을 고정시킨 후에 atomic force microscopy를 이용하여 표면을 관찰하였다. 클라미디아 균에 대한 항체를 투여하고 나서 자체 제작한 SPR 센서를 이용하여 항원 항체 반응을 SPR 파장 변화로 측정하였다. 결과 : 양전하를 띤 PDDA 표면위에서 클라미디아 균이 고정되었음을 확인 하였다. 그리고, 항체를 투여한 후에 SPR 파장의 증가를 확인하였다. 파장 변화는 항원의 농도와 관련이 있었다. 결론 : 전하를 이용하여 클라미디아 폐렴균의 EB를 단백질 칩에 고정하였고, 단백질 칩 위에서의 항원 항체 반응을 확인하였다. 비정형 폐렴의 진단에 SPR 센서가 기여할 수 있을 것으로 사료되나, 실제 임상 시료에의 적용을 위해서는 좀더 연구가 필요할 것으로 사료된다.

• 한국안전학회지
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• 제29권4호
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• pp.9-14
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• 2014

Self-piercing riveting(SPR) is a mechanical fastening technique which is put pressure on the rivet for joining the sheets. Unlike a spot welding, SPR joining does not make the harmful gas and $CO_2$ and needs less energy consumption. In this study, static and fatigue tests were conducted using tensile-shear specimens with Al-5052 plates for evaluation of fatigue strength of the SPR joints. During SPR joining process for the specimen, using the current sheet thickness and a rivet, the optimal applied punching force was found to be 21 kN. And, the maximum static strength of the specimen produced at the optimal punching force was 3430 N. During the fatigue tests for the specimens, interface failure mode occurred on the top substrate close to the rivet head in the most high-loading range region, but on the bottom substrate close to the rivet tail in the low -loading range region. There was a relationship between applied load amplitude $P_{amp}$ and lifetime of cycle N for the tensile-shear, $P_{amp}=3395.5{\times}N^{-0.078}$. Using the stress-strain curve of the Al-5052 from tensile test, the simulations for fatigue specimens have been carried out using the implicit finite element code ABAQUS. The relation between von-Mises equivalent stress amplitude and number of cycles was found to be ${\sigma}_{eq}=514.7{\times}N^{-0.033}$.

• 분석과학
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• 제18권6호
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• pp.460-468
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• 2005

The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. $60{\AA}$ thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and $7.5{\times}10^{-12}mol/cm^2$, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

• 센서학회지
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• 제20권6호
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• pp.434-438
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• 2011

A portable surface plasmon resonance(SPR) based immunosensor using thiolated protein G and protein G was developed for the detection of immunoglobulin G(IgG). The protein G has specific affinity with Fc fragment of IgG and was thiolated by 2-Iminothiolane for introduction of thiol groups. Anti-IgG, bovine serum albumin(BSA), and IgG have been sequently injected after surface modification of gold sensor chip with protein G and thiolated protein G. The output signal was increased with the injection of each protein and the actual signal was measured by subtracting signal of reference channel from signal of sample injected channel. The experimental results showed the higher detection capability of IgG using thiolated protein G compared with protein G. From these results, we can conclude that the current surface modification technique and the portable SPR sensor system can be applied to various immunosensors for diagnosis.

• Journal of the Optical Society of Korea
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• 제13권3호
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• pp.392-397
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• 2009

We designed a wavelength interrogation-based surface plasmon resonance (SPR) biosensor to detect avian influenza DNA (AI-DNA). Hybridization reactions between target AI-DNA probes and capture probes immobilized on a gold surface were monitored quantitatively by measuring the resonance wavelength in the visible waveband. The experimental results were consistent with numerical calculations. Although the SPR detection technique does not require the DNA to be labeled, we also evaluated fluorescently-labeled targets to verify the hybridization behavior of the AI-DNA. Changes in resonance were found to be linearly proportional to the amount of bound analyte. A wavelength interrogation-type SPR biosensor can be used for rapid measurement and high-throughput detection of highly pathogenic AI viruses.

• 한국콘텐츠학회논문지
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• 제16권8호
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• pp.209-216
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• 2016

강력범죄사건에서 발견할 수 있는 증거물인 테이프는 접착면에 지문이 쉽게 유류될 수 있다. 청 테이프에 유류된 지문을 현출하고자 사삼산화철($Fe_3O_4$)기반의 Small Particle Reagents(SPR)를 사용하여 SPR의 제조비율 및 현출에 영향을 주는 요인에 대해 연구하였다. SPR이 지문현출에 영향을 주는 요인은 제조시 첨가되는 분말의 양, 계면활성제의 양, 계면활성제의 HLB(hydrophile-lipophile balance)에 의해 영향을 받는다. 증류수 100ml를 기준으로 분말의 양은 0.5g~1.0g일 때 융선에 적절한 양이 흡착되었으며 계면활성제의 양은 0.5ml이상이면 배경에 흡착된 분말들을 잘 씻어냈다. 또한 계면활성제의 HLB가 배경과 융선과의 대조비를 높이는 중요한 요인이었으며 유류된 지문의 지질 성분을 용해시키지 않는 11~18사이의 HLB가 뛰어난 현출력을 보였다.

• 한국항공우주학회지
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• 제48권8호
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• pp.597-609
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• 2020

동축 로터 시스템은 기존의 헬리콥터뿐만 아니라 드론, PAV, 차세대 고속 회전익기 및 화성탐사용 헬리콥터에 적용되는 등, 활용 분야가 점점 넓어지고 있다. 로터 시스템의 성능 연구는 기존 연구에서 동축 로터를 대상으로도 여러 차례 수행되었으나, 로터 블레이드의 변형에 대한 연구는 주로 단일 로터 시스템만을 대상으로 진행되었다. 하지만 동축 로터 시스템에서는 주변에서 발생하는 유동이 복잡하며, 두 로터의 간격이 로터 시스템 전체의 성능에 주요한 영향을 주므로 블레이드의 변형 연구가 더욱 중요하다. 이에 본 연구에서는 최신식 고속 영상촬영 기법 중 하나인 SPR(Stereo Pattern Recognition) 기법을 사용해 동축 로터 블레이드의 변형 측정에 대한 실험적 연구를 수행하였다. 본 연구에는 한국항공우주연구원에서 개발한 축소 동축 로터 시험장치가 사용되었으며, 로터 블레이드의 변형과 성능의 연관성을 고찰하기 위해 동축 로터 시스템의 성능시험이 선행되었다. 해당 시스템의 로터 블레이드 변형을 SPR 기법으로 측정한 결과는 사전에 진행된 성능시험 결과와 함께 본 논문에 제시되었다.