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http://dx.doi.org/10.5369/JSST.2011.20.6.434

Detection of IgG Using Thiolated Protein G Modified SPR Sensor Chip  

Sin, Eun-Jung (Department of Biomedical Engineering, Catholic University of Daegu)
Lee, Yeon-Kyung (Department of Biomedical Engineering, Catholic University of Daegu)
Sohn, Young-Soo (Department of Biomedical Engineering, Catholic University of Daegu)
Publication Information
Journal of Sensor Science and Technology / v.20, no.6, 2011 , pp. 434-438 More about this Journal
Abstract
A portable surface plasmon resonance(SPR) based immunosensor using thiolated protein G and protein G was developed for the detection of immunoglobulin G(IgG). The protein G has specific affinity with Fc fragment of IgG and was thiolated by 2-Iminothiolane for introduction of thiol groups. Anti-IgG, bovine serum albumin(BSA), and IgG have been sequently injected after surface modification of gold sensor chip with protein G and thiolated protein G. The output signal was increased with the injection of each protein and the actual signal was measured by subtracting signal of reference channel from signal of sample injected channel. The experimental results showed the higher detection capability of IgG using thiolated protein G compared with protein G. From these results, we can conclude that the current surface modification technique and the portable SPR sensor system can be applied to various immunosensors for diagnosis.
Keywords
Surface Plasmon Resonance; Immunosensor; Protein G; Thiolation; Immunoglobulin G;
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Times Cited By KSCI : 5  (Citation Analysis)
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