• Title/Summary/Keyword: SPR technique

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p-Adaptive Mesh Refinement of Plate Bending Problem by Modified SPR Technique (수정 SPR 기법에 의한 휨을 받는 평판문제의 적응적 p-체눈 세분화)

  • Jo, Jun-Hyung;Lee, Hee-Jung;Woo, Kwang-Sung
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2007.04a
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    • pp.481-486
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    • 2007
  • The Zienkiewicz-Zhu(Z/Z) error estimate is slightly modified for the hierarchical p-refinement, and is then applied to L-shaped plates subjected to bending to demonstrate its effectiveness. An adaptive procedure in finite element analysis is presented by p-refinement of meshes in conjunction with a posteriori error estimator that is based on the superconvergent patch recovery(SPR) technique. The modified Z/Z error estimate p-refinement is different from the conventional approach because the high order shape functions based on integrals of Legendre polynomials are used to interpolate displacements within an element, on the other hand, the same order of basis function based on Pascal's triangle tree is also used to interpolate recovered stresses. The least-square method is used to fit a polynomial to the stresses computed at the sampling points. The strategy of finding a nearly optimal distribution of polynomial degrees on a fixed finite element mesh is discussed such that a particular element has to be refined automatically to obtain an acceptable level of accuracy by increasing p-levels non-uniformly or selectively. It is noted that the error decreases rapidly with an increase in the number of degrees of freedom and the sequences of p-distributions obtained by the proposed error indicator closely follow the optimal trajectory.

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Measurement of Aircraft Wing Deformation and Vibration Using Stereo Pattern Recognition Method (스테레오 영상을 이용한 비행 중인 항공기 날개의 변위 및 진동 측정)

  • Kim, Ho-Young;Yoon, Jong-Min;Han, Jae-Hung;Kwon, Hyuk-Jun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.25 no.8
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    • pp.568-574
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    • 2015
  • The present study was conducted by using stereo pattern recognition method(SPR method) to measure the displacement and vibration of an airplane wing in flight condition. A SPR based measurement system was developed using two visible light stereo cameras. The visible light stereo images were processed to obtain marker points by adaptive threshold method and marker filtering technique. The marker points were used to reconstruct 3D point, displacement, and vibration data. The SPR system was installed on F-16 fighter. The wing displacement and vibration were measured in flight condition. Therefore, this paper presents a possibility that SPR based measurement system using visible light stereo camera can be very useful for measuring displacement and vibration of an airplane in flight condition.

Development of the Real-time Concentration Measurement Method for Evaporating Binary Mixture Droplet using Surface Plasmon Resonance Imaging (표면플라즈몬공명 가시화 장치를 이용한 증발하는 이종혼합물 액적의 실시간 농도 가시화 기법 개발)

  • Jeong, Chan Ho;Lee, Hyung Ju;Choi, Chang Kyoung;Lee, Hyoungsoon;Lee, Seong Hyuk
    • Journal of ILASS-Korea
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    • v.26 no.4
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    • pp.212-218
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    • 2021
  • The present study aims to develop the Surface Plasmon Resonance (SPR) imaging system facilitating the real-time measurement of the concentration of evaporating binary mixture droplet (BMD). We introduce the theoretical background of the SPR imaging technique and its methodology for concentration measurement. The SPR imaging system established in the present study consists of a LED light source, a polarizer, a lens, and a band pass filter for the collimated light of a 589 nm wavelength, and a CCD camera. Based on the Fresnel multiple-layer reflection theory, SPR imaging can capture the change of refractive index of evaporating BMD. For example, the present study exhibits the visualization process of ethylene glycol (EG)-water (W) BMD and measures real-time concentration change. Since the water component is more volatile than the ethylene glycol component, the refractive index of EG-W BMD varies with its mixture composition during BMD evaporation. We successfully measured the ethylene glycol concentration within the evaporating BMD by using SPR imaging.

Development of Protein Chip for Diagnosis of Chlamydophia Pneumoniae (단백질 칩을 이용한 클라미디아 폐렴의 진단)

  • Kim, Woo Jin;Lee, Hui Young;Lee, Seung-Joon;Jung, Se-Hui;Yuk, Jong Seol;Ha, Kwon-Soo;Jung, Ki-Suck
    • Tuberculosis and Respiratory Diseases
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    • v.60 no.4
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    • pp.412-418
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    • 2006
  • Background; The diagnosis of chlamydial infection is based on serology. The current gold standard of diagnosis is MIF(microimmunofluorescence), but this modality is subjective and time-consuming. Protein microarray with using a SPR(surface plasmon resonance) sensor has recently been suggested as a method for detecting infection. For developing a protein chip to diagnose chlamydial infection, EBs(elementary bodies) were immobilized on a gold chip and the interaction between an antibody for Chlamydophila pneumoniae and the EBs(elementary bodies) immobilized on the surface of the gold chip was measured by using an SPR sensor. Methods; For the surface antigen, the EBs of Chlamydophila pneumoniae LKK1 were purified. Charged arrays were prepared by using PDDA(polydiallyldimethylammonium chloride) which has a positive charge. After immobilization of the chlamydial EBs on the PDDA surface, the investigation of the surface was done with using atomic force microscopy. After the antibody for C. pneumoniae was applied on chip, we monitored the SPR wavelength-shift to detect any antigen-antibody interaction with using a self-assembled SPR sensor. Results; The chlamydial EBs on the positively charged PDDA were visible on the surface with using atomic force microscopy. The SPR wavelength increased after interaction of antibody for C. pneumoniae with the EBs immobilized on charged gold surface. The wavelength-shift was correlated with the concentration of antigens. Conclusion; The surface immobilization of EBs on the gold surface with the charged arrays was identified and the antigen-antibody interaction on the gold chip was detected via the SPR sensor. Further investigations are needed to apply this technique to the clinical field.

Fatigue Strength of Al-5052 Tensile-Shear Specimens using a SPR Joining Method (SPR 접합법을 이용한 Al-5052 인장-전단 시험편의 피로강도)

  • Lee, Man Suk;Kim, Taek Young;Kang, Se Hyung;Kim, Ho Kyung
    • Journal of the Korean Society of Safety
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    • v.29 no.4
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    • pp.9-14
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    • 2014
  • Self-piercing riveting(SPR) is a mechanical fastening technique which is put pressure on the rivet for joining the sheets. Unlike a spot welding, SPR joining does not make the harmful gas and $CO_2$ and needs less energy consumption. In this study, static and fatigue tests were conducted using tensile-shear specimens with Al-5052 plates for evaluation of fatigue strength of the SPR joints. During SPR joining process for the specimen, using the current sheet thickness and a rivet, the optimal applied punching force was found to be 21 kN. And, the maximum static strength of the specimen produced at the optimal punching force was 3430 N. During the fatigue tests for the specimens, interface failure mode occurred on the top substrate close to the rivet head in the most high-loading range region, but on the bottom substrate close to the rivet tail in the low -loading range region. There was a relationship between applied load amplitude $P_{amp}$ and lifetime of cycle N for the tensile-shear, $P_{amp}=3395.5{\times}N^{-0.078}$. Using the stress-strain curve of the Al-5052 from tensile test, the simulations for fatigue specimens have been carried out using the implicit finite element code ABAQUS. The relation between von-Mises equivalent stress amplitude and number of cycles was found to be ${\sigma}_{eq}=514.7{\times}N^{-0.033}$.

Characterization of biotin-avidin recognition system constructed on the solid substrate

  • Lim, Jung-Hyurk
    • Analytical Science and Technology
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    • v.18 no.6
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    • pp.460-468
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    • 2005
  • The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. $60{\AA}$ thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and $7.5{\times}10^{-12}mol/cm^2$, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

Detection of IgG Using Thiolated Protein G Modified SPR Sensor Chip (Thiolated protein G로 개질된 SPR 센서 칩을 이용한 IgG 검출)

  • Sin, Eun-Jung;Lee, Yeon-Kyung;Sohn, Young-Soo
    • Journal of Sensor Science and Technology
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    • v.20 no.6
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    • pp.434-438
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    • 2011
  • A portable surface plasmon resonance(SPR) based immunosensor using thiolated protein G and protein G was developed for the detection of immunoglobulin G(IgG). The protein G has specific affinity with Fc fragment of IgG and was thiolated by 2-Iminothiolane for introduction of thiol groups. Anti-IgG, bovine serum albumin(BSA), and IgG have been sequently injected after surface modification of gold sensor chip with protein G and thiolated protein G. The output signal was increased with the injection of each protein and the actual signal was measured by subtracting signal of reference channel from signal of sample injected channel. The experimental results showed the higher detection capability of IgG using thiolated protein G compared with protein G. From these results, we can conclude that the current surface modification technique and the portable SPR sensor system can be applied to various immunosensors for diagnosis.

Detection of Avian Influenza-DNA Hybridization Using Wavelength-scanning Surface Plasmon Resonance Biosensor

  • Kim, Shin-Ae;Kim, Sung-June;Lee, Sang-Hun;Park, Tai-Hyun;Byun, Kyung-Min;Kim, Sung-Guk;Shuler, Michael L.
    • Journal of the Optical Society of Korea
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    • v.13 no.3
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    • pp.392-397
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    • 2009
  • We designed a wavelength interrogation-based surface plasmon resonance (SPR) biosensor to detect avian influenza DNA (AI-DNA). Hybridization reactions between target AI-DNA probes and capture probes immobilized on a gold surface were monitored quantitatively by measuring the resonance wavelength in the visible waveband. The experimental results were consistent with numerical calculations. Although the SPR detection technique does not require the DNA to be labeled, we also evaluated fluorescently-labeled targets to verify the hybridization behavior of the AI-DNA. Changes in resonance were found to be linearly proportional to the amount of bound analyte. A wavelength interrogation-type SPR biosensor can be used for rapid measurement and high-throughput detection of highly pathogenic AI viruses.

The Factors Influencing Latent Fingermark Development on Adhesive Side of Iron Oxide Powder-based Small Particle Reagent (사삼산화철 기반의 소립자시약(Small Particle Reagent)의 접착면 잠재지문 현출 효과에 영향을 미치는 요인)

  • Kim, Sun-Min;Go, Gang-Seok;Lee, Seul-Bi;Yu, Je-Seol
    • The Journal of the Korea Contents Association
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    • v.16 no.8
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    • pp.209-216
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    • 2016
  • Latent fingerprint left on the adhesive sides of tapes can be easily found at a crime scene. Small Particle Reagents(SPR) based on iron oxide($Fe_3O_4$) is a technique for the detection of a latent fingerprint adhesive surface. In this study, found out that the causes affecting the quality of the fingerprints developed when used SPR based on iron oxide. To a suspension of 0.5g of iron oxide in 100ml of distilled water, 0.5ml or more surfactant were added can be developed latent fingerprints of good quality. In addition, using surfactants HLB(hydrophile-lipophile balance) value of 11~18 showed good contrast to the background and latent fingerprint.

An Experimental Study on Blade Deformation of Coaxial Rotor System Using SPR(Stereo Pattern Recognition) Technique (SPR(Stereo Pattern Recognition) 기법을 이용한 동축 로터 블레이드의 변형에 대한 실험적 연구)

  • Yoo, Chanho;Yoon, Byung-Il;Chae, Sanghyun;Kim, Do-Hyung;Kim, Deog-Kwan
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.48 no.8
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    • pp.597-609
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    • 2020
  • These days, the coaxial rotor system is used for various purposes like UAVs, Mars exploration helicopters, and the next-generation high-speed rotorcraft. A number of research projects on aerodynamic performance of rotor systems, including the coaxial configuration have been made previously. On the contrary, research on rotor blade deformation has been mainly carried out regarding the single rotor system, where such effort has not been enough on the coaxial system. Nonetheless, in case of the coaxial system, blade deformation analysis is much more important because of the complex air flow around the rotors, and that the distance between the two rotors is a key factor affects aerodynamic performance of the entire system. For these reasons, an experimental study on rotor blade deformation of the coaxial system was conducted using the Stereo Pattern Recognition(SPR) technique, one of the state-of-the-art of photogrammetry method. In this research, a small-scale coaxial rotor test stand designed by Korea Aerospace Research Institute(KARI) was used. With the same test stand, performance of the coaxial configuration had been studied before the experimental study on blade deformation, in order to find the relation between performance and blade deformation of the rotor system. Results of the performance test and the deformation study are presented in this article.