• 한국자원식물학회지
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• 제24권3호
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• pp.272-279
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• 2011

It is very crucial to evaluate the genetic diversity of peanut genetic resources for identification of peanut germplasm accessions and variety improvement. Cultivated peanut generally has two subspecies, hypogaea and fastigiata. In this study, we identified peanut into three plant types, virginia (var. hypogaea), spanish (var. vulgaris), and valencia (var. fastigiata). Former one belongs to ssp. hypogaea and latter two are involved in ssp. fastigiata. Twenty SSR markers were used to assess the genetic variation of three sets, hypogaea, vulgaris, and fastigiata, respectively. Out of variety-specific SSR primers tried in this study, ten pairs of SSR primers showed polymorphisms. Each accession could be identified by a specific set of polymorphic SSR primers, and allele number was evaluated among accessions, with an average of 6.7 in var. hypogaea and 5.4 in var. vulgaris and fastigiata. For evaluation of genetic diversity, gene diversity ranged from 0.336 to 0.844 and PIC (polymorphism information contents) ranged from 0.324 to 0.827 were investigated. Dendrograms based on genetic distances were constructed, which showed the existence of three different clusters. And these three different clusters might be associated with the genes involved in three plant types. The results also suggested that there were plentiful SSR polymorphisms among peanut germplasm accessions in RDA (Rural Development Administration, Korea) Genebank and SSRs might play an important role in evaluating peanut accessions and cultivar improvement.

• 생명과학회지
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• 제20권12호
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• pp.1764-1771
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• 2010

홍화(Carthamus tinctorius L.)는 세계 여러 나라에 분포하고 있는 초본류이다. 이 종은 경제적으로 중요한데 홍화는 약용, 적색소, 노랑 색소로 이용된다. RAPD 기법으로 홍화의 26 집단 간 유연관계와 유전적 다양성을 조사하였다. 모든 집단에서 123개 밴드를 얻었으며 시발체(primer) 당 평균 9.5개 밴드를 나타내었다. 홍화의 유전적 다양도는 집단 내에 대부분 귀속되며 높은 집단 간 분화를 나타내었다. OPC18-01 밴드는 시리아 그룹에 특이 밴드였으며 다른 나라 집단에서는 발견되지 않았다. 이런 7개 특이 마크(SCAR)를 발견하였다. 비록 홍화의 분석한 개체 수가 적고 각 나라의 대표성을 의미하지 않지만 본 연구 결과 지중해의 지역(모로코, 시리아, 터키)이 인도를 제외한 다른 지역보다 변이가 높았다. 단순히 RAPD만으로 단정하기 어렵지만 홍화의 기원 센터의 후보군으로 지중해 연안으로 추정된다. 인도 역시 홍화의 2차 센터의 후보군이다. RAPD 마커는 홍화의 자연 집단을 분류하는데 효과적이었다.

• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.567-573
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• 2011

We investigated the phylogenetic diversity of ammoniaoxidizing bacteria (AOB) in Yellow Sea continental shelf sediment by the cloning and sequencing of PCR-amplified amoA and 16S rRNA genes. Phylogenetic analysis of the amoA-related clones revealed that the diversity of AOB was extremely low at the study site. The majority (92.7%) of amoA clones obtained belonged to a single cluster, environmental amoA cluster-3, the taxonomic position of which was previously unknown. Phylogenetic analysis on AOB-specific 16S rRNA gene sequences also demonstrated a very low diversity. All of the cloned 16S rRNA gene sequences comprised a single phylotype that belonged to the members of uncultured Nitrosospira cluster-1, suggesting that AOB belonging to the uncultured Nitrosospira cluster-1 could carry amoA sequences of environmental amoA cluster-3.

• 한국약용작물학회지
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• 제13권5호
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• pp.262-269
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• 2005

The objective of this study was to assess genetic diversity among 6 different wild ginseng populations from New York, Kentucky, North Carolina, Pennsylvania, Tennessee and Virginia, and to compare these wild populations to one cultivated population. RAPD markers were used to estimate the genetic difference among samples from the 7 populations. The 64 random primers were screened, and 15 primers were selected which exhibited the 124 highly reproducible polymorphic markers. The ratio of discordant bands to total bands scored was used to estimate the genetic distance within and among populations. Multidimensional scaling (MDS) of the relation matrix showed distinctive separation between wild and cultivated populations. The MDS result was confirmed using pooled chi-square tests for fragment homogeneity. This study suggests that RAPD markers can be used as population-specific markers for American ginseng.

• Asian-Australasian Journal of Animal Sciences
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• 제22권7호
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• pp.955-961
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• 2009

The aim of this study was to determine the specific polymorphic sites in cattle breeds and inter- and interbreed genetic variation among breeds and to develop a databank of Turkish native cattle mtDNA using sequence analysis. The entire D-loop region was analyzed based on DNA sequences in Turkish Grey, East Anatolian Red, South Anatolian Red, and Anatolian Black native breeds. In total, 68 nucleotide differences were observed at 26 different sites. The variable positions consisted of 22 transitions, two transversions, and two insertions, but no deletions. Haplotype number, haplotype diversity, nucleotide diversity, and mean number of pairwise difference values were found to be 17, 0.993, 0.00478, and 4.275, respectively. In addition, a phylogeny was developed by comparison among cattle populations for which the entire D-loop sequence was available. A high level of genetic variation was observed within and among the native cattle breeds.

• Mass Spectrometry Letters
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• 제7권3호
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• pp.74-78
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• 2016

Targeted glycoproteomics is an effective way to discover disease-associated glycoproteins in proteomics and serial affinity chromatography (SAC) using lectin and glycan-targeting antibodies shows glycan diversity on the captured glycoproteins. This study suggests a way to determine glycan heterogeneity and structural analysis on the post-translationally modified proteins through serial affinity column set (SACS) using four Lycopersicon esculentum lectin (LEL) columns. The great advantage of this method is that it differentiates between glycoproteins on the basis of their binding affinity. Through this study, some proteins were identified to have glycoforms with different affinity on a single glycoprotein. It will be particularly useful in determining biomarkers in which the disease-specific feature is a unique glycan, or a group of glycans.

• 한국통신학회논문지
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• 제27권6B호
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• pp.569-578
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• 2002

This paper proposes a new multicarrier code division multiple access (CDMA) system. The proposed multicarrier CDMA system provides the advantages that the transmission bandwidth is more efficiently utilized by using divided spreading sequence, time and frequency diversity is achieved in frequency selective nultipath (acting channel, and inter-carrier interference (ICI) can be minimized by using specific data and code pattern. In this system, transmitted data bits are serial-to-parallel converted to some parallel branches. On each branch each bit is direct-sequence spread-spectrum modulated by divided spreading sequences and transmitted using orthogonal carriers. The receiver providers a Rake for each carrier, and the outputs of Rakes are combined to get time and frequency diversity. This multicarrier CDMA system allows additional flexibility in the choice of system parameters. Upon varying system parameters, bit error rate (BER) performance is examined for the proposed multicarrier CDMA system. Simulation results show that the proposed multicarrier CDMA scheme can achieve better performance than the other types of conventional multicarrier CDMA systems.

• Current Research on Agriculture and Life Sciences
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• 제22권
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• pp.29-39
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• 2004

This study was conducted to provide a fundamental information for commercial, medical usage and mushroom gene preservation and to investigate the diversity of indigenous mushroom on Bonghwa-Gun. A total of 3 classes 2 subclasses 13 orders 45 families 64 genera and 118 species including saprophytic and ectomycorrhizal fungi was investigated. The mushroooms are classified into 17 families 32 genera and 80 species in Agaricales, 13 families 17 genera and 22 species in Aphllophorales, 2 families 2 genera and 2 species in Heterobasidiomycetes, 9 families 9 genera and 10 species in Gasteromycetidae. There were edible mushroom of 46 species at Moonsu and Chungryang Mountain. The mushroom occurrence was closely related to vegetation and soil environmental conditions.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.124-127
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• 2023

Among certain animals, gut microbiomes demonstrate species-specific patterns of beta diversity. This host-specificity is a potent driver of exogenous microbial exclusion. To overcome persistent translational limitations, translational microbiome research and therapeutic development must account for host-specific patterns of microbial engraftment. This commentary seeks to highlight the important implications of host-specificity for microbial ecology, Fecal Microbiota Transplantation (FMT), next-generation probiotics, and translational microbiota research.

• Journal of Contemporary Eastern Asia
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• 제16권1호
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• pp.22-45
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• 2017

The successful management of software (SW) projects is a continuous concern to managers, which is attributed to the contradictory demands that most projects are facing; meeting user requirements within time and budget limit while flexibly dealing risks during the progress of the projects. The present study asserts project performance and risk mitigation are not tradeoff but to be achieved simultaneously, which is called SW project ambidexterity. Drawing on the literature on organizational behavior, hypotheses are developed speculating the relation among project performance, risk mitigation, team collective improvisation, team behavioral integration, and team diversity. Using empirical data collected from 102 SW project teams of 507 team members in South Korea, empirical analysis indicates team collective improvisation is a significant antecedent to SW project ambidexterity, playing a pivotal role to balance the contradictory demands. Furthermore, team behavioral integration positively influences the degree of team collective improvisation, and the magnitude of the relation is partially contingent on the team members' age and major diversity. The present study advances theory by providing a context specific explanation about the SW project ambidexterity and its precedents.