• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2290-2294
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• 2014

For the present study, rhEGF was encapsulated into solid lipid nanoparticles (SLNs). The SLNs were prepared by the $W_1/O/W_2$ double emulsification method combined with the high pressure homogenization method and the physical properties such as particle size, zeta-potential and encapsulation efficiency were measured. The overall particle morphology of SLNs was investigated using a transmission electron microscopy (TEM). The percutaneous skin permeation and accumulation property of rhEGF was evaluated using Franz diffusion cell system along with confocal laser scanning microscopy (CLSM). The mean particle size of rhEGF-loaded SLNs was $104.00{\pm}3.99nm$ and the zeta-potential value was in the range of -$36.99{\pm}0.54mV$, providing a good colloidal stability. The TEM image revealed a spherical shape of SLNs about 100 nm and the encapsulation efficiency was $18.47{\pm}0.22%$. The skin accumulation of rhEGF was enhanced by SLNs. CLSM image analysis provided that the rhEGF rat skin accumulation is facilitated by an entry of SLNs through the pores of skin.

• Asian Pacific Journal of Cancer Prevention
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• 제17권sup3호
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• pp.317-321
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• 2016

Detection of micrometastasis in sentinel lymph nodes (SLNs) is a very useful tool for appropriate assessment of the clinical stage of disease in breast cancer patients. Early identification of clinically relevant disease could lead to early treatment or staging approaches for breast cancer patient. Micrometastases in SLNs of women with invasive breast cancer are of great significance in this context. In this study we examined SLN biopsies considered to have small numbers of cancerous cells by real time RT-PCR. All of the samples underwent immunohistochemical staining for cytokeratin for confirmation of the presence or absence of micrometastases. BUB1b expression assay of selected patients with and without metastasis showed overexpression in the former, but not in normal breast and lymph node tissue. Our results may be taken into account in the discussion about the merits of routine use of molecular assessment in pathogenetic studies of SLNs.

• 대한방사성의약품학회지
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• 제1권1호
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• pp.62-73
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• 2015

We developed an evans blue-indocyanine green-$^{99m}Tc$-human serum albumin conjugate for sentinel lymph node mapping and we describe its unique potential usage for clinical implications. This conjugate has combined the strengths of visible blue dye, near-infrared fluorescence and radioisotope into one single conjugate without any additional weakness/disadvantage. All the components of evans blue-indocyanine green-$^{99m}Tc$-human serum albumin are safe and of low cost, and they have already been clinically used. This conjugate was stable in the serum, it showed a long retention time in the lymphatic system and the lymph nodes showed a much higher signal-to-noise ratio after the conjugate was injected intradermally into the paw of mice. Both the single-photon emission computed tomography and near-infrared fluorescent images of the mice were successfully obtained at the same time as the excised sentinel lymph nodes showed blue color. The visual color, near-infrared fluorescence and gamma ray from this agent could be complementary for each other in all the steps of sentinel lymph node sampling: exploring and planning sentinel lymph node before excision with visualization of the exact sentinel lymph node location during an operation. Therefore, the triple modal agent will possibly be very ideal for sentinel lymph node mapping because of the high signal-to-noise ratio for non-invasive imaging and its complementary multimodal nature, easy preparation and safety. It is promising for clinical applications and it may have great advantages over the traditional single modal methods.

• International Journal of Oral Biology
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• 제45권1호
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• pp.25-31
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• 2020

Enamel knot (EK)-a signaling center-refers to a transient morphological structure comprising epithelial tissue. EK is believed to regulate tooth development in early organogenesis without its own cellular alterations, including proliferation and differentiation. EKs show a very simple but conserved structure and share functions with teeth of recently evolved vertebrates, suggesting conserved signaling in certain organs, such as functional teeth, through the course of evolution. In this study, we examined the expression patterns of key EK-specific genes including Dusp26, Fat4, Meis2, Sln, and Zpld1 during mice embryogenesis. Expression patterns of these genes may reveal putative differentiation mechanisms underlying tooth morphogenesis.

• 한국정보과학회논문지:소프트웨어및응용
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• 제34권7호
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• pp.585-594
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• 2007

오류역전파 방법을 이용하는 신경망들은 패턴들의 학습시간이 매우 오래 걸리고 또한 추가학습과 반복학습의 한계를 가지며, 이런 단점을 보완할 수 있는 이진신경망(Binary Neural Network, BNN)이 Aleksander에 의해 제안되었다. 그러나 BNN도 반복학습에 있어서는 단점을 가지고 있으며, 일반화 패턴을 추출하기 어렵다. 본 논문에서는 BNN의 구조를 개선하여 반복학습과 추가학습이 가능할 뿐 아니라, 특징점들까지 추출할 수 있는 다중 판별자를 가지는 삼차원 뉴로 시스템을 제안한다. 제안된 모델은 기존의 BNN을 기반으로 하여 만들어진 이차원 특징을 가지는 Single Layer Network(SLN)에 귀환회로가 추가되어 특징점들을 누적할 수 있는 삼차원 신경망이다. 학습을 통해 누적된 정보는 판별자의 각 신경세포에 임계치를 조정함으로써 일반화 패턴을 추출할 수 있다. 그리고 생성된 일반화 패턴을 인식에 재사용함으로써 반복학습의 효율성을 높였다. 최종 판정 단계에서는 Maximum Response Detector(MRD)를 이용하였다. 본 논문에서 제안한 시스템을 평가하기 위하여 NIST에서 제공하는 숫자 자료를 이용하였으며, 99.3%의 인식률을 얻었다.

• 대한방사성의약품학회지
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• 제5권1호
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• pp.54-60
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• 2019

Sentinel lymph node (SLN) imaging plays an important role in surgery of patients with breast cancer and melanoma. In this study, avidin (Av), a tetrameric protein glycosylated with mannose and N-acetylglucosamine molecules, was labeled with $^{64}Cu$ and then evaluated for LN imaging. $^{64}Cu$-Labeled $NeutrAvidin^{TM}$ (NAv), a non-glycosylated form of Av, was used for comparison. 1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)-conjugated Av and NAv were prepared from the corresponding proteins and DOTA-NHS ester, which were then labeled with copper-64 and purified using PD-10 columns. The numbers of DOTA molecules conjugated to Av and NAv were 4.9 and 3.3, respectively. [$^{64}Cu$]Cu-DOTA-conjugated Av and NAv were prepared in 93% and 73% radiochemical yields, respectively. In vitro serum stability study showed that copper-64 remained stable on all radiotracers for 24 h (>97%). MicroPET/CT images showed that high radioactivity was accumulated in LNs within 15 min after footpad-injection of radiotracers. Tissue distribution data of mice demonstrated significantly higher uptake in the popliteal (PO) LN than lumbar (LU) LN for $^{64}Cu$-labeled Av (relative % ID/g excluding the injection sites: 66.2% and 26.0%, respectively) compared with those of $^{64}Cu$-labeled NAv (43.0% and 49.2%, respectively). The results of this study suggest that mannose molecules on Av enabled the radiotracer to retain in the first LN after mouse footpad-injection.

• The Journal of Advanced Prosthodontics
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• 제11권1호
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• pp.65-74
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• 2019

PURPOSE. To evaluate and compare the effect of different materials and techniques on the shear bond strength of veneering ceramic materials to zirconia. MATERIALS AND METHODS. 136 sintered zirconia cubes were prepared and randomly divided into four study groups according to corresponding methods of surface treatment and materials: GLN (grinding followed by laser scanning using Noritake Cerabien ZR), SLN (sandblasting followed by laser scanning using Noritake Cerabien ZR), GLV (grinding followed by laser scanning using VITA VM 9), and SLV (sandblasting followed by laser scanning using VITA VM 9). Spraying technique was performed to coat the core. Profilometer, SEM, XRD, EDS, universal testing machine, and stereomicroscope were used to record surface roughness Ra, surface morphology, phase transformation, elemental compositions, shear bond strength SBS values, and failure types, respectively. Specimens were investigated in unaged (not immersed in artificial saliva) and aged (stored in artificial saliva for a month) conditions to evaluate SBS values. RESULTS. Grinding and GLN as first and second surface treatments provided satisfactory Ra values in both conditions ($1.05{\pm}0.24{\mu}m$, $1.30{\pm}0.21{\mu}m$) compared to sandblasting and other groups (P<.05). The group GLN showed the highest SBS values in both conditions ($30.97{\pm}3.12MPa$, $29.09{\pm}4.17MPa$), while group SLV recorded the lowest ($23.96{\pm}3.60MPa$, $22.95{\pm}3.68Mpa$) (P<.05). Sandblasting showed phase transformation from t-m. Mixed failure type was the commonest among all groups. CONCLUSION. GLN showed to be a reliable method which provided satisfactory bond strength between the veneer ceramic and zirconia. This method might preserve the integrity of fixed dental crowns.

• 대한의용생체공학회:의공학회지
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• 제37권1호
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• pp.7-14
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• 2016

Radiopharmaceutical agents for positron emission tomography (PET), such as $^{18}F$-FDG and $^{68}Ga$, have been used not only for whole-body PET imaging but also for intraoperative radionuclide-guided surgery due to their quantitative and sensitive imaging characteristics. Current intraoperative probes detect gamma or beta particles, but not both of them. Gamma probes have low sensitivities since a collimator has to be used to reduce backgrounds. Positron probes have a high tumor-to-background ratio, but they have a 1-2 mm depth limitation from the body surface. Most of current intraoperative probes produce only audible sounds proportional to count rates without providing tumor images. This research aims to detect both positrons and annihilation photons from $^{18}F$ using plastic scintillators and a GAGG scintillation crystal attached to silicon photomultiplier (SiPM). The depth-of-interaction (DOI) along the plastic scintillator can be used to obtain the 2-D images of tumors near the body surface. The front and rear part of the intraoperative probe consists of $4{\times}1$ plastic scintillators ($2.9{\times}2.0{\times}12.0mm^3$) for positron detection and a Ce:GAGG scintillation crystal ($12.0{\times}12.0{\times}9.0mm^3$) for annihilation photon detection, respectively. The DOI resolution of $4.4{\pm}1.6mm$ along the plastic scintillator was obtained by using the 3M enhanced specular reflector (ESR) with rectangular holes between the plastic scintillators, which showed the feasibility of a 2-D image pixel size of $2.9{\times}4.4mm^2$ (X-direction ${\times}$ Y-direction).

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10451-10456
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• 2015

Background: We defined melanoma distribution in a large series of Turkish patients and evaluated the prognostic parameters of melanomas. Materials and Methods: A total of 1574 patients' data was retrospectively collected at 18 centers in Turkey. Demographic characteristics were questioned and noted. Prognostic parametres were evaluated based on sentinel lymph node involvement. Results: Mean age was 56.7 (4-99) years. While 844 (53.6%) cases were male, 730 (46.4%) cases were female. One thousand four hundred forty-seven (92%) cases were invasive melanoma and 127 (8%) cases were in-situ melanoma. The most common histopathological form was the superficial spreading melanoma (SSM) which was found in 549 patients (37.9%). It was followed by nodular melanoma in 379 (26.2%), acral lentiginous melanoma (ALM) in 191 (13.2%) and lentigo maligna melanoma in 132 (9.1%), respectively. On univariate analysis, lymphovascular invasion (p<0.001), tumor thickness (p<0.001), histopathological subtype (p<0.001), Clark level (p=0.001), ulceration (p<0.001), ${\geq}6/mm^2$ mitosis (p=0.005), satellite formation (p=0.001) and gender (p=0.03) were found to be associated with sentinel lymph node positivity. Regression was associated with sentinel lymph node negativity (p=0.017). According to multivariate analysis, lymphovascular invasion and tumor thickness were significant independent predictive factors of SLN positivity. Patient age, tumor localization, precursor lesions, lymphocytic infiltration and neurotropism were not related with sentinel lymph node involvement. Conclusions: In this retrospective analysis, it was found that the prevalence of SSM is at a lower rate while the prevalence of ALM is at a higher rate when compared to western countries. According to Breslow index; most of the melanoma lesions' thickness were greater than 2 mm, corresponding Clark IV. Vascular invasion and tumor thickness are the most important factors for sentinel lymph node involvement.

• 한국언론정보학보
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• 제27권
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• pp.283-316
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• 2004

본 연구는 국책사업인 핵폐기장 유치와 관련해 지역간 경쟁과정에서 나타난 갈등적 이슈들을 해당 지역신문들이 어떻게 다루고 있는지를 뉴스프레임 분석을 통해 고찰했다. 연구대상인 광주일보, 매일신문, 전북일보의 뉴스기사 분석결과, 핵폐기장이라는 같은 사건에 대해 각각의 신문들이 사용하는 주요 프레임이 상이하게 나타났다. 우선 광주일보와 매일신문에서는 대항 프레임이 높게 사용되었으며, 이와 달리 전북일보에서는 지역발전 프레임이 가장 높게 집계되었다. 또한 헤드라인 주제어와 뉴스프레임 구성은 깊은 연관성이 있는 것으로 밝혀졌다. 광주일보와 매일신문의 대항프레임 기사에서는 헤드라인 주제어로 핵폐기장 사용 빈도가 높았으며, 지역발전 프레임의 전북일보기사에서는 헤드라인 주제어로 방사성폐기장이 압도적으로 높게 나타났다. 정부의 '양성자'와 '핵폐기장' 연계정책 발표 이후에도 광주일보와 매일신문에서의 뉴스 프레임은 그 이전과 비교하여 변화가 거의 없었다. 이에 반해 전북일보에서는 연계 정책 발표 이후, 대항 프레임이 현격히 감소한 대신에 정책 프레임은 크게 증가한 것으로 집계되었다. 동시에 전북일보의 헤드라인 주제어 사용에서 핵폐기장은 급격하게 감소하였으며, 방사성폐기장은 급격하게 증가한 것으로 나타났다. 이러한 결과에서 전북일보는 헤드라인 주제어로 방사성폐기장을 즐겨 사용하여 핵폐기장의 부정적 이미지를 최소화하고, 핵폐기장 유치가 곧 지역발전이라는 여론을 조성한 것이라 할 수 있다. 언론이 구성한 세계가 현실 그 자체가 된다는 그간의 프레임 연구 결과들은 당시 전북 부안으로 핵폐기장 유치가 결정된 것은 결코 우연이 아님을 보여주고 있다.