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http://dx.doi.org/10.7314/APJCP.2016.17.S3.317

Targeting of BUB1b Gene Expression in Sentinel Lymph Node Biopsies of Invasive Breast Cancer in Iranian Female Patients  

Mansouri, Neda (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital)
Movafagh, Abolfazl (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital)
Sayad, Arezou (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital)
Pour, Atefeh Heidary (Department of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University)
Taheri, Mohammad (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital)
Soleimani, Shahrzad (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital)
Mirzaei, Hamid Reza (Department of Radiation Oncology, Cancer Research Center, Shohadae Tajrish Hospital)
Shargh, Shohreh Alizadeh (Department of Medical Laboratory Science, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch)
Azargashb, Eznollah (Department of Epidemiology, School of Medicine, Shahid Beheshti University of Medical Sciences)
Bazmi, Haleh (Department of Biology Laboratory Science, Islamic Azad University of Roudehen Branch)
Moradi, Hossein Allah (Medical research fellow)
Zandnia, Fatemeh (Department of Biology, Shahre kord University of Islamic Azad University)
Hashemi, Mehrdad (Department of Molecular Genetics, Islamic Azad University)
Massoudi, Nilofar (Department of Anesthesiology, Shahid Beheshti University of Medical Sciences)
Mortazavi-Tabatabaei, SA (Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.17, no.sup3, 2016 , pp. 317-321 More about this Journal
Abstract
Detection of micrometastasis in sentinel lymph nodes (SLNs) is a very useful tool for appropriate assessment of the clinical stage of disease in breast cancer patients. Early identification of clinically relevant disease could lead to early treatment or staging approaches for breast cancer patient. Micrometastases in SLNs of women with invasive breast cancer are of great significance in this context. In this study we examined SLN biopsies considered to have small numbers of cancerous cells by real time RT-PCR. All of the samples underwent immunohistochemical staining for cytokeratin for confirmation of the presence or absence of micrometastases. BUB1b expression assay of selected patients with and without metastasis showed overexpression in the former, but not in normal breast and lymph node tissue. Our results may be taken into account in the discussion about the merits of routine use of molecular assessment in pathogenetic studies of SLNs.
Keywords
Breast cancer; micro-metastasis; sentinel lymph node; real time PCR; Iranian population;
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