Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Targeting of BUB1b Gene Expression in Sentinel Lymph Node Biopsies of Invasive Breast Cancer in Iranian Female Patients

  • Mansouri, Neda (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital) ;
  • Movafagh, Abolfazl (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital) ;
  • Sayad, Arezou (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital) ;
  • Pour, Atefeh Heidary (Department of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University) ;
  • Taheri, Mohammad (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital) ;
  • Soleimani, Shahrzad (Department of Medical Genetics, School of Medicine, Shohadae Tajrish Hospital) ;
  • Mirzaei, Hamid Reza (Department of Radiation Oncology, Cancer Research Center, Shohadae Tajrish Hospital) ;
  • Shargh, Shohreh Alizadeh (Department of Medical Laboratory Science, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch) ;
  • Azargashb, Eznollah (Department of Epidemiology, School of Medicine, Shahid Beheshti University of Medical Sciences) ;
  • Bazmi, Haleh (Department of Biology Laboratory Science, Islamic Azad University of Roudehen Branch) ;
  • Moradi, Hossein Allah (Medical research fellow) ;
  • Zandnia, Fatemeh (Department of Biology, Shahre kord University of Islamic Azad University) ;
  • Hashemi, Mehrdad (Department of Molecular Genetics, Islamic Azad University) ;
  • Massoudi, Nilofar (Department of Anesthesiology, Shahid Beheshti University of Medical Sciences) ;
  • Mortazavi-Tabatabaei, SA (Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences)
  • Published : 2016.06.01
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Abstract

Detection of micrometastasis in sentinel lymph nodes (SLNs) is a very useful tool for appropriate assessment of the clinical stage of disease in breast cancer patients. Early identification of clinically relevant disease could lead to early treatment or staging approaches for breast cancer patient. Micrometastases in SLNs of women with invasive breast cancer are of great significance in this context. In this study we examined SLN biopsies considered to have small numbers of cancerous cells by real time RT-PCR. All of the samples underwent immunohistochemical staining for cytokeratin for confirmation of the presence or absence of micrometastases. BUB1b expression assay of selected patients with and without metastasis showed overexpression in the former, but not in normal breast and lymph node tissue. Our results may be taken into account in the discussion about the merits of routine use of molecular assessment in pathogenetic studies of SLNs.

Keywords

References

  1. Baker DJ, Jin F, Jeganathan KB, et al (2009). Whole chromosome instability caused by Bub1 insufficiency drives tumorigenesis through tumor suppressor gene loss of heterozygosity. Cancer Cell, 16, 475-86. https://doi.org/10.1016/j.ccr.2009.10.023
  2. Bonora M, Wieckowsk MR, Chinopoulos C, et al (2015). Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene, 34, 1608. https://doi.org/10.1038/onc.2014.462
  3. Colak D, Nofal A, AlBakheet A (2013). Age-specific gene expression signatures for breast tumors and cross-species conserved potential cancer progression markers in young women. culhane AC, ed. PLoS ONE, 8, e63204. https://doi.org/10.1371/journal.pone.0063204
  4. Daniele L, Annaratone L, Allia E, et al (2009). Technical limits of comparison of step-sectioning,immunohistochemistry and RT-PCR on breast cancer sentinel nodes: a study on methacarn-fixed tissue. J Cell Mol Med, 13, 4042-50. https://doi.org/10.1111/j.1582-4934.2008.00449.x
  5. Early Breast Cancer Trialists' Collaborative Group (EBCTCG) (2005). Effects of chemotherapy and hormonal therapy for early breast cancer on recurrence and15-year survival: an overview of the randomised trials. Lancet, 65, 1687-1717.
  6. Ellis MJ, Coop A, Singh B, et al (2001). Letrozole is more effective neoadjuvant endocrine therapy than tamoxifen for ErbB-1- and/or ErbB-2-positive, estrogen receptor-positive primary breast cancer: evidence from a phase III randomized trial. J Clin Oncol, 19, 3808-16. https://doi.org/10.1200/JCO.2001.19.18.3808
  7. Fardmanesh H, Shekari M, Movafagh A, et al (2016). Upregulation of the double-stranded RNA binding protein DGCR8 in invasive ductal breast carcinoma. Gene, 58, 146-51.
  8. Freedman RA, Kouri EM, West DW, et al (2015). Higher stage of disease is associated with bilateral mastectomy among patients with breast cancer: a population-based survey. Clin Breast Cancer, 16, 105-12.
  9. Gerber B, Heintze K, Stubert J, et al (2011). Axillary lymph node dissection in early-stage invasive breast cancer: is it still standard today? Breast Cancer Res Treat, 128, 613-24. https://doi.org/10.1007/s10549-011-1532-0
  10. Gillanders WE, Mikhitarian K, Hebert R, et al (2004). Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg J, 239, 828-37. https://doi.org/10.1097/01.sla.0000128687.59439.d6
  11. Goetz MP, Suman VJ, Ingle JN, et al (2006). A two-gene expression ratio of homeobox 13 and interleukin-17B receptor for prediction of recurrence and survival in women receiving adjuvant tamoxifen. Clin Cancer Res, 12, 2080-7. https://doi.org/10.1158/1078-0432.CCR-05-1263
  12. Guillen-Paredes MP, Carrasco-Gonzalez L, Chaves-Benito A, et al (2011). [One-step nucleic acid amplification (OSNA) assay for sentinel lymph node metastases as an alternative to conventional postoperative histology in breast cancer: A cost-benefit analysis]. Cir Esp, 89, 456-62. https://doi.org/10.1016/j.ciresp.2011.04.013
  13. Habel LA, Sakoda LC, Achacoso N, et al (2013). HOXB13:IL17BR and molecular grade index and risk of breast cancer death among patients with lymph node-negative invasive disease. Breast Cancer Res, 15, R24. https://doi.org/10.1186/bcr3402
  14. Heidari MH, Porghasem M, Mirzaei N, et al (2014). The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran. J Environ Radioact, 128, 64-7. https://doi.org/10.1016/j.jenvrad.2013.11.001
  15. Jaffrey RG, Pritchard SC, Clark C, et al (2000). Genomic instability at the BUB1 locus in colorectal cancer, but not in non-small cell lung cancer. Cancer Res, 60, 4349-52.
  16. Ma XJ, Salunga R, Dahiya S, et al (2008) . A five-gene molecular grade index and HOXB13:IL17BR are complementary prognostic factors in early stage breast cancer. Clin Cancer Res, 14, 2601-8. https://doi.org/10.1158/1078-0432.CCR-07-5026
  17. Movafagh A, Maleki F, Fadaie S, et al (2007). Persistent unstable chromosomal aberration in lymphocytes of radiotherapy workers after 1st mitotic division in Tehran, Iran. PJMS, 23, 254-60.
  18. Movafagh A, Mirfakhraei R, Mousavi-Jarrahi A et al (2011). Frequent incidence of double minute chromosomes in cancers, with special up-to-date reference to leukemia. Asian Pac J Cancer Prev, 12, 3453-6.
  19. Movafagh A, Hajifathali A, Isfahani F, et al (2012). Geographic heterogeneity of cytogenetic characteristics of acute myeloid leukemia in the early detection. Iran J Cancer Prev, 2, 85-9.
  20. Movafagh A, Hajifathali A, Zamani M (2011). Secondary chromosomal bnormalities of de novo acute myeloid leukemia-a first report from the Middle East. Asian Pac J Cancer Prev, 12, 2991-4.
  21. Nishidate T, Katagiri T, Lin ML, et al (2004). Genome-wide gene-expression profiles of breast-cancer cells purified with laser microbeam microdissection: identification of genes associated with progression and metastasis. Int J Oncol, 25, 797-819.
  22. Ouellette RJ, Richard D, Maicas E (2004). RT-PCR for mammaglobin genes, MGB1 and MGB2, identifies breast cancer micrometastases in sentinel lymph nodes. Am J Clin Pathol, 121, 637-43. https://doi.org/10.1309/MMACTXT55L8QTKC1
  23. Pappa KI, Rodolakis A, Christodoulou I, et al (2014). Comparative assessment of lymph node micrometastasis in cervical, endometrial and vulvar cancer: insights on the real time qRT-PCR approach versus immunohistochemistry, employing dual molecular markers. Biomed Res Int, 187684.
  24. Patani N, Mokbel K (2011). Clinical significance of sentinel lymph node isolated tumour cells in breast cancer. Breast Cancer Res Treat, 127, 325-34. https://doi.org/10.1007/s10549-011-1476-4
  25. Shargh SA, Sakizli M, Khalaj V, et al (2014). Downregulation of E-cadherin expression in breast cancer by promoter hypermethylation and its relation with progression and prognosis of tumor. Med Oncol, 31, 250. https://doi.org/10.1007/s12032-014-0250-y
  26. Seifi-Alan M, Shamsi R, Ghafouri-Fard S, et al (2014). Expression analysis of two cancer-testis genes, FBXO39 and TDRD4, in breast cancer tissues and cell lines. Asian Pac J Cancer Prev, 14, 6625-9.
  27. Varma N, Varma S, Movafagh A, et al (1995). Unusual clonal cytogenetic abnormalities in aplastic anemia. Am J Hematol, 49, 256-7. https://doi.org/10.1002/ajh.2830490319
  28. Wang Y, Klijn JG, Zhang Y, et al (2005). Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer. Lancet, 365, 671-9. https://doi.org/10.1016/S0140-6736(05)70933-8
  29. Wang Z, Spaulding B, Sienko A, et al (2004). Mammaglobin, a valuable diagnostic marker for metastatic breast carcinoma. Int J Clin Exp Pathol, 2, 384-9.

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