• Title/Summary/Keyword: SL

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Lipase-Catalyzed Synthesis of Structured Lipids with Capric and Conjugated Linoleic Acid in a Stirred-Batch Type Reactor (대두유로부터 Lipase를 이용한 재구성 지질의 합성 및 특성)

  • 신정아;이기택
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1175-1179
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    • 2004
  • Structured lipid (SL) was produced from soybean oil with molar ratio of 1:2:2 (soybean oil:capric acid:CLA) using Chirazyme L-2 lipase (4% by weight of total substrates). The reaction was conducted for 24 hr at 55$^{\circ}C$ in a 1 L stirred-batch type reactor. SL-soybean oil contained 4.9 mol% capric acid and 4.1 mol% CLA, respectively. Iodine value of SL-soybean oil was reduced than that of soybean oil due to the incorporated capric acids. Tocopherol content in SL-soybean oil was 18.2 mg/l00 g. SL-soybean oil appeared more yellowish color than soybean oil. Reverse-phase HPLC showed that SL-triacylglycerol species containing capric acid consisted of about 12.6 area%.

Investigation of Eye-Tracking on Learning Task Perceiving Process of Elementary Students with Different Motivation System on Science Learning (학습과제 인지 과정에 대한 과학학습 동기체계에 따른 초등학생의 시선이동 분석)

  • Yang, Il-Ho;Lim, Sung-Man;Kim, Yang-Hee
    • Journal of Korean Elementary Science Education
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    • v.34 no.1
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    • pp.86-94
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    • 2015
  • This study is to investigate how do elementary students' eye movements appear in cognitive process that they perceive science learning materials depending on motivation system of science learning. For this study we had a random sampling of 301, 6th grade students. And we had selected 32 students through the three tests for the final; motivation system test of science learning, a learning styles test, an Edinburgh inventory for handedness test. We were analysis the cognitive process during learning tasks for the selected student using eye-tracking equipment. The results of the research are as follows: First, when students see a science learning material, we found out that SL-BAS group that has tendency searched various areas than SL-BIS group in learning task. Second, results confirmed through the data of integrational count that students looked alternately texts, images and graphs in the learning material, SL-BIS group were more than SL-BAS group on integrated count, but they had a simple and insignificant eye movement. Especially SL-BAS students showed that integrated eye movement between texts, images and graphs in the learning material, and they explored important areas of the graph compared with SL-BIS group that there was no eye movement in graph. These results may be utilized as a useful resource for designing students' learning.

The use of SlAdh2 promoter as a novel fruit-specific promoter in transgenic tomato

  • Chung, Mi-Young;Naing, Aung Htay;Vrebalov, Julia;Shanmugam, Ashokraj;Lee, Do-Jin;Park, In Hwan;Kim, Chang Kil;Giovannon, James
    • Journal of Plant Biotechnology
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    • v.47 no.2
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    • pp.172-178
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    • 2020
  • Fruit-specific promoters play an important role in the improvement of traits, such as fruit quality through genetic engineering. In tomato, the development of fruit-specific promoters was previously reported, but less attention has been paid to the promoters involved in the fruit development stage. In this study, we characterized the gene expression patterns of tomato alcohol dehydrogenase 2 (SlAdh2) in various tissues of wild-type tomato (cv. Ailsa Craig). Our findings revealed that SlAdh2 expression levels were higher in the developing fruit than in the leaves, stems, and flowers. The ProSlAdh2 region, which is expressed at different stages of fruit development, was isolated from tomato genomic DNA. Following this, it was fused with a β-glucuronidase reporter gene (GUS) and introduced into wild-type tomato using Agrobacterium-mediated transformation to evaluate promoter activity in the various tissues of transgenic tomato. The ProSlAdh2:GUS promoter exhibited strong activity in the fruit and weak activity in the stems, but displayed undetectable activity in the leaves and flowers. Interestingly, the promoter was active from the appearance of the green fruit (1 cm in size) to the well-ripened stage in transgenic tomatoes, indicating its suitability for transgene expression during fruit development and ripening. Thus, our findings suggest that ProSlAdh2 may serve as a potential fruit-specific promoter for genetic-based improvement of tomato fruit quality.

Preliminary Survey of Age and Growth of the Short-necked Clam, Paphia undalate(Born), in Kwangyang Bay, Korea (광양만에 분포하는 농조개, Paphia undalata (Born)의 연령과 성장에 관한 기초연구)

  • 김영혜;장대수;박영철
    • The Korean Journal of Malacology
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    • v.17 no.1
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    • pp.7-12
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    • 2001
  • The age and growth of the short-necked clam, Paphia undalata, was investigated from 546 samples randomly collected in December 2000 in Kwangyang Bay, Korea. Ages were determined from ring radius of shell and the maximum age was observed to be 2 years. The relationship between shell length (SL) and shell height (SH) of Paphia undalata was SL = 0.2105 + 1.7569 $\times$ SH ($R^2$= 0.98), and the shell length (SL)-total weight (TW) relationship was TW = 2.5824 $\times$ 10$^{-4}$ $\times$ S $L^{2.6769}$ ($R^2$= 0.92). The von Bertalanffy growth parameters were estimated by the non-linear method, with values as follows: $L_{\infty}$ = 81.46 mm, K : 0.20/year, $t_{0}$ = -1.19 year. The von Bertalnanffy growth equation was $L_{t}$ = 81.46(1- $e^{-0}$.20(t+1.19)/), $W_{t}$ = 33.68(1- $e^{-0}$.20(t+1.19)/)$^{2.6769}$.

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Cis-acting Elements in the 3' Region of Potato virus X are Required for Host Protein Binding

  • Kwon, Sun-Jung;Kim, Kook-Hyung;Hemenway Cynthia
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.139-146
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    • 2006
  • The 3' region of Potato virus X (PVX) has the 74 nt 3'-nontranslated region (NTR) that is conserved among all potexviruses and contains several cis-acting elements for minus-strand and plus-strand RNA accumulation. Three stem-loop structures (SL1-SL3), especially formation of SL3 and U-rich sequence of SL2, and near upstream elements in the 3' NTR were previously demonstrated as important cis-acting elements. To Investigate the binding of these cis-acting elements within 3' end with host protein, we used the electrophoretic mobility shift assays (EMSA) and UV-cross linking analysis. The EMSA with cellular extracts from tobacco and RNA transcripts corresponding to the 150 nt of the 3' end of PVX RNA showed that the 3' end of PVX formed complexes with cellular proteins. The specificity of protein binding was confirmed through competition assay by using with 50-fold excess of specific and non-specific probes. We also conducted EMSA with RNAs containing various mutants on those cis-acting elements (${\Delta}10$10, SL3B, SL2A and ${\Delta}21$; J Mol Biol 326, 701-720) required for efficient PVX RNA accumulation. These analyses supported that these cis-acting elements are required for interaction with host protein(s). UV-cross linking analysis revealed that at least three major host proteins of about 28, 32, and 42 kDa in mass bound to these cis-elements. These results indicate that cis-acting elements from 3' end which are important for minus and plus-strand RNA accumulation are also required for host protein binding.

Weed Occurrence and Control in Simultaneous Wheat Sowing Culture with Rice Harvest under No-tilled Paddy Field (무경운(無耕耘) 벼 수확동시(收穫同時) 밀 파종에서 잡초(雜草)발생과 방제)

  • Kim, Dae-Ho;Kim, Su-Kyeong;Kim, Eun-Seok;Son, Beom-Young;Kang, Dong-Ju
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.186-190
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    • 1998
  • To establish weed control method in no-tilled and rice straw-covered wheat sown coincidentally with rice harvest using combine-attached drill seeder, several herbicides and their combination were tested for 2 years. Similar number of panicle to hand-weeding plot was secured at sprayed plot with Buta EC + Glyphosate SL mixture just after seeding. Water-foxtail(Alopeculus aequalis) and broadleaf weeds were controlled effectively by Buta EC + Glyphosate SL mixture, showing the control efficacy of 92 percent. In the 1st cropping year, weed species occurred was simple and water-foxtail predominated nearly half. However, weed species was diversified and water-foxtail predominated 83.5 percent in the 2nd cropping year. Wheat yield of Buta EC + Glyphosate SL plot showed no difference with hand-weeding plot. Dry weight of water-foxtail showed closer linear relationship with wheat yield reduction than that of broadleaf weed.

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Amorphous Cr-Ti Texture-inducing Layer Underlying (002) Textured bcc-Cr alloy Seed Layer for FePt-C Based Heat-assisted Magnetic Recording Media

  • Jeon, Seong-Jae;Hinata, Shintaro;Saito, Shin
    • Journal of Magnetics
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    • v.21 no.1
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    • pp.35-39
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    • 2016
  • $Cr_{100-x}Ti_x$ amorphous texture-inducing layers (TIL) were investigated to realize highly (002) oriented $L1_0$ FePt-C granular films through hetero-epitaxial growth on the (002) textured bcc-$Cr_{80}Mn_{20}$ seed layer (bcc-SL). As-deposited TILs showed the amorphous phase in Ti content of $30{\leq}x(at%){\leq}75$. Particularly, films with $40{\leq}x{\leq}60$ kept the amorphous phase against the heat treatment over $600^{\circ}C$. It was found that preference of the crystallographic texture for bcc-SLs is directly affected by the structural phase of TILs. (002) crystallographic texture was realized in bcc-SLs deposited on the amorphous TILs ($40{\leq}x{\leq}70$), whereas (110) texture was formed in bcc-SLs overlying on crystalline TILs (x < 30 and x > 70). Correlation between the angular distribution of (002) crystal orientation of bcc-SL evaluated by full width at half maximum of (002) diffraction (FWHM) and a grain diameter of bcc-SL indicated that while the development of the lateral growth for bcc-SL grain reduces FWHM, crystallization of amorphous TILs hinders FWHM. $L1_0$ FePt-C granular films were fabricated under the substrate heating process over $600^{\circ}C$ with having different FWHM of bcc-SL. Hysteresis loops showed that squareness ($M_r/M_s$) of the films increased from 0.87 to 0.95 when FWHM of bcc-SL decreased from $13.7^{\circ}$ to $3.8^{\circ}$. It is suggested that the reduction of (002) FWHM affects to the overlying MgO film as well as FePt-C granular film by means of the hetero-epitaxial growth.

Envelope-Function Equation and Motion of Wave Packet in a Semiconductor Superlattice Structure

  • Kim, Byoung-Whi;Jun, Young-Il;Jung, Hee-Bum
    • ETRI Journal
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    • v.21 no.1
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    • pp.1-27
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    • 1999
  • We present a new description of envelope-function equation of the superlattice (SL). The SL wave function and corresponding effective-mass equation are formulated in terms of a linear combination of Bloch states of the constituent material with smaller band gap. In this envelope-function formalism, we review the fundamental concept on the motion of a wave packet in the SL structure subjected to steady and uniform electric fields F. The review confirms that the average of SL crystal momentums K = ($k_x,k_y,q$), where ($K_x,k_y$) are bulk inplane wave vectors and q SL wave vector, included in a wave packet satisfies the equation of motion = $_0+Ft/h$; and that the velocity and acceleration theorems provide the same type of group velocity and definition of the effective mass tensor, respectively, as in the Bulk. Finally, Schlosser and Marcus's method for the band theory of metals has been by Altarelli to include the interface-matching condition in the variational calculation for the SL structure in the multi-band envelope-function approximation. We re-examine this procedure more thoroughly and present variational equations in both general and reduced forms for SLs, which agrees in form with the proposed envelope-function formalism. As an illustration of the application of the present work and also for a brief investigation of effects of band-parameter difference on the subband energy structure, we calculate by the proposed variational method energies of non-strained $GaAs/Al_{0.32}Ga_{0.68}As$ and strained $In_{0.63}Ga_{0.37}As/In_{0.73}Ga_{0.27}As_{0.58}P_{0.42}SLs$ with well/barrier widths of $60{\AA}/500{\AA}$ and 30${\AA}/30{\AA}$, respectively.

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Age and Growth of the Stone Flounder Rareius Bocolortus in Approaches to Kyongyolto of Yellow Sea Korea (한국 서해안 격열비열도 근해산 동가자미의 연령과 성장)

  • 박종수
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.2
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    • pp.121-126
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    • 1995
  • Age and growth of stone flounder kareius bicolortus in approaches to Kyongyolbiyolto of Yellow sea were studied by otolith reading of 248 fishes from September 1992 to August 1993. Examination of the ourter margins of the otoliths showed that th hyaline zone as annulus was formed once a year and that its formation was completed at the end of the spawning season(Jan. to Feb.). Growth of the fish was expressed by the von Bertalanffy's equation as Lt=313.7[1-exp{-0.463(t+0.0704)}] for males and Lt=478.6[1-exp{-0.286(t-0.1619)}] for females, where Lt is body length in mm and It is age in years. It was found that growth of males and females differs, with the females showing a higher growth than the males. Growth in body weight was also examined. The oldest fish were seven years old for males and females.

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Variance component analysis of growth and production traits in Vanaraja male line chickens using animal model

  • Ullengala, Rajkumar;Prince, L. Leslie Leo;Paswan, Chandan;Haunshi, Santosh;Chatterjee, Rudranath
    • Animal Bioscience
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    • v.34 no.4
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    • pp.471-481
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    • 2021
  • Objective: A comprehensive study was conducted to study the effects of partition of variance on accuracy of genetic parameters and genetic trends of economic traits in Vanaraja male line/project directorate-1 (PD-1) chicken. Methods: Variance component analysis utilizing restricted maximum likelihood animal model was carried out with five generations data to delineate the population status, direct additive, maternal genetic, permanent environmental effects, besides genetic trends and performance of economic traits in PD-1 chickens. Genetic trend was estimated by regression of the estimated average breeding values (BV) on generations. Results: The body weight (BW) and shank length (SL) varied significantly (p≤0.01) among the generations, hatches and sexes. The least squares mean of SL at six weeks, the primary trait was 77.44±0.05 mm. All the production traits, viz., BWs, age at sexual maturity, egg production (EP) and egg weight were significantly influenced by generation. Model four with additive, maternal permanent environmental and residual effects was the best model for juvenile growth traits, except for zero-day BW. The heritability estimates for BW and SL at six weeks (SL6) were 0.20±0.03 and 0.17±0.03, respectively. The BV of SL6 in the population increased linearly from 0.03 to 3.62 mm due to selection. Genetic trend was significant (p≤0.05) for SL6, BW6, and production traits. The average genetic gain of EP40 for each generation was significant (p≤0.05) with an average increase of 0.38 eggs per generation. The average inbreeding coefficient was 0.02 in PD-1 line. Conclusion: The population was in ideal condition with negligible inbreeding and the selection was quite effective with significant genetic gains in each generation for primary trait of selection. The animal model minimized the over-estimation of genetic parameters and improved the accuracy of the BV, thus enabling the breeder to select the suitable breeding strategy for genetic improvement.