• Asian-Australasian Journal of Animal Sciences
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• v.33 no.10
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• pp.1566-1572
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• 2020

Objective: The extensive breeding of commercial chickens has led to a sharp decrease in the resources of many indigenous chickens, especially the indigenous chickens in the southeastern coastal region, which are on the verge of extinction, and the indigenous chickens in the northwestern region of China, which are also at risk. However, there are few reports on the evaluation of genetic diversity and conservation of genetic resources of indigenous chickens in remote areas in the Northwest of China. Methods: In the present study, the genetic diversity and phylogenetic relationship of six indigenous chickens from different regions were studied based on variation in mitochondrial DNA control region (D-loop), and the degree of introgression from commercial breeds into these chickens was determined by the amount of haplotype sharing between indigenous and commercial breeds. Results: Twenty-five polymorphic sites and 25 haplotypes were detected in 206 individuals. Principal component analysis showed that the Jingning chicken had the highest genetic diversity among the six indigenous chickens. According to the degree of introgression, the six indigenous breeds may be involved in haplotype sharing with commercial breeds, and the introgression from commercial chickens into the Haidong chicken is the most serious. Conclusion: The genetic uniqueness of indigenous chickens has been eroded, so it is necessary to consider the protection of their genetic resources. Phylogenetic analysis suggests that the six indigenous chickens have two major matrilineal origins: one from Yunnan or its surrounding areas in China and the other from the Indian subcontinent.

• BMB Reports
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• v.38 no.4
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• pp.386-390
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• 2005

Based on the reported cDNA sequences of $BmK{\alpha}Txs$, the genes encoding toxin $BmK{\alpha}Tx11$ and $BmK{\alpha}Tx15$ were amplified by PCR from the Chinese scorpion Buthus martensii Karsch genomic DNA employing synthetic oligonucleotides. Sequences analysis of nucleotide showed that an intron about 500 bp length interrupts signal peptide coding regions of $BmK{\alpha}Tx11$ and $BmK{\alpha}Tx15$. Using cDNA sequence of $BmK{\alpha}Tx11$ as probe, southern hybridization of BmK genome total DNA was performed. The result indicates that $BmK{\alpha}Tx11$ is multicopy genes or belongs to multiple gene family with high homology genes. The similarity of $BmK{\alpha}$-toxin gene sequences and southern hybridization revealed the evolution trace of $BmK{\alpha}$-toxins: $BmK{\alpha}$-toxin genes evolve from a common progenitor, and the genes diversity is associated with a process of locus duplication and gene divergence.

• Journal of Korea Water Resources Association
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• v.41 no.2
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• pp.173-184
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• 2008

Climatic water balance has been applied to obtain quantity of various hydrologic components. Hydrologic information is estimated by comparison between rainfall and evapotranspiration under complex terrain condition. Water deficit is defined as that subtraction of actual supply from climatic demand. The water deficit will occur, when monthly evapotranspiration exceed monthly rainfall. Contrary water surplus is defined as that surplus water after meeting the demand by plants. The water surplus will be occurred when monthly rainfall exceeds monthly evapotranspiration. Finally, the discrete moisture indices were calculated and mapped for the whole watershed to estimate dryness and wetness status using the climatic water balance approach. The result of this study can properly interpret the real drought and non drought. Based upon the results, it can be concluded that the climatic water balance model is useful to monitor water conditions for the watershed.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.12
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• pp.5990-6008
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• 2019

Recognizing license plate from its traffic camera captured images is one of the most important aspects in many traffic management systems. Despite many sophisticated license plate recognition related algorithms available online, license plate recognition is still a hot research issue because license plates in each country all round the world lack of uniform format and their camera captured images are often affected by multiple adverse factors, such as low resolution, poor illumination effects, installation problem etc. A novel method is proposed in this paper to solve the inclination problem of license plates in their camera captured images through four parts: Firstly, special edge pixels of license plate are chosen to represent main information of license plates. Secondly, least square methods are used to compute the inclined angle of license plates. Then, coordinate rotation methods are used to rotate the license plate. At last, bilinear interpolation methods are used to improve the performance of license plate rotation. Several experimental results demonstrated that our proposed method can solve the inclination problem about license plate in visual aspect and can improve the recognition rate when used as the image preprocessing method.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.42 no.1
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• pp.250-256
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• 2017

Recently, the use of mobile devices has increased in order to provide a variety of services, and thus there has been a surge in the number of application malicious attacks on the Android platform. To resolve the problem, the domestic financial sector has been introducing the app anti-tamper solution based on cryptographic algorithms. However, since the capacity of apps installed in smartphones continues to increase and environments with limited resources as wearables and IoTs spread, there are limitations to the processing speed of the anti-tamper solutions. In this paper, we propose a novel anti-tamper solution by using lightweight hash function LEA and LSH. We also present the test results of a simulation program that implements this method and compare the performance with anti-tamper solutions based on the previous cryptographic algorithms.

• Journal of the Optical Society of Korea
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• v.20 no.3
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• pp.389-395
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• 2016

A Carbon Fiber Composite (CFC) framework was designed for a small lightweight space camera. According to the distribution characteristics of each optical element in the optical system, CFC (M40J) was chosen to accomplish the design of the framework. TC4 embedded parts were used to solve the low accuracy of the CFC framework interface problem. An integrated optimization method and the optimization strategy which combined a genetic global optimization algorithm with a downhill simplex local optimization algorithm were adopted to optimize the structure parameters of the framework. After optimization, the total weight of the CFC framework and the TC4 embedded parts is 15.6 kg, accounting for only 18.4% that of the camera. The first order frequency of the camera reaches 104.8 Hz. Finally, a mechanical environment test was performed, and the result demonstrates that the first order frequency of the camera is 102 Hz, which is consistent with the simulation result. It further verifies the rationality and correctness of the optimization result. The integrated optimization method mentioned in this paper can be applied to the structure design of other space cameras, which can greatly improve the structure design efficiency.

• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.19-25
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• 2014

An extracellular agarase was purified from Bacillus sp. BI-3, a thermophilic agar-degrading bacterium isolated from a hot spring in Indonesia. The purified agarase revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular mass of 58 kDa. The optimum pH and temperature of the agarase were 6.4 and $70^{\circ}C$, respectively. The activity of the agarase was stable at high temperatures, and more than 50% activity was retained at $80^{\circ}C$ for 15 min. Furthermore, the enzyme was stable in the pH range of 5.8-8.0, and more than 60% of the residual activity was retained. Significant activation of the agarase was observed in the presence of $K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, and $Sr^{2+}$; on the other hand, $Ba^{2+}$, $Zn^{2+}$, $Cu^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Fe^{2+}$, and EDTA inhibited or inactivated the enzyme activity. The components of the hydrolytic product analyzed by thin-layer chromatography showed that the agarase mainly produced neoagarobiose. This study is the first to present evidence of agarolytic activity in aerobic thermophilic bacteria.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1908-1917
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• 2016

Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. The cultivation time also decreased from 54 h to 36 h. Compared with the wild strain, a dramatic increase in enzyme activity was observed for the mutant strain, although the increase in biomass was limited. DNA and amino acid sequence alignment revealed 11 nucleotide substitutions and 10 amino acid substitutions between the sldAB of strains L-6 and I-2-239. Simulation of the 3-D structure and prediction of active site residues and PQQ binding site residues suggested that these mutations were mainly related to PQQ binding, which was speculated to be favorable for the catalyzing capacity of glycerol dehydrogenase. RT-qPCR assay indicated that the transcription levels of sldA and sldB in the mutant strain were respectively 4.8-fold and 5.4-fold higher than that in the wild strain, suggesting another possible reason for the increased DHA productivity of the mutant strain.

• Journal of Haehwa Medicine
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• v.4 no.2
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• pp.333-333
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• 1996

Chinese medicine is a precious treasure inherited from ancient ancestors. It is accredited for the prosperous growth of the Chinese nations. However, the descriptions of the herbs in the ancient herbal are not in detail and the great numbers of herbs used which grows in wide geographic areas together with various local folk names, new substitutes and new folk medicines had increased, many Chinese herbs are composed of herbs that are labeled with identical names but actually are of different origins and different grades. Similar situation had occurred in China, japan and Korea In Taiwan, misused Chinese crude drugs are also very common in the past. This phenomenon had caused a lot of confusion and had great influence the clinical efficacy of the treatment. In the past, Professor Hong Yen Hsu, Na Chi, Woei Song Kan and Kung Yin Yen had studied the origins of Chinese crude drugs in Taiwan based on the morphological identification and found that the origins of Ma-Tou-Ling, Pu-Kung-Yin, Tu-Chung, Wang-Pu-Liu-Hsing, Pan-lan-Ken, Niu-Chi, Fang-Chi, Huang-Chi, PienHsiu and Sha Wan-Tzi are different from that of the species used in mainland China. In order to assure the quality and clinical efficacy of the crude drugs, besides the traditional morphological methods, we bad recently combined modem chemical and pharma-cological methods to assess drug quality. Drugs that have been evaluated without effects should be abandoned. The species of those commonly misued crude drugs used in compound formula preparations are also identified Based on the pharmacological results, a suitable species is recommended so as to improve the clinical efficacy of those preparations. In this paper, we like to report our recent studies on Niu Chi(Achyranthis Bidentatae Radix, Cyathulae Radix and Strobilanthis Radix). Fang-Chi(Arstolochiae Fangchi Radix, Stephaniae Tetrandrae Radix and Cocculus Radix) and Huang-Chi(Astragali Radix and Hedysari Radix) using comparative pharmacognosy methods.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.41 no.10
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• pp.69-75
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• 2004

This work proposes an 8b 220 MS/s 230 mW 3-stage pipeline CMOS ADC with on-chip filers for temperature- and power- insensitive voltage references. The proposed RC low-pass filters improve switching noise performance and reduce reference settling time at heavy R & C loads without conventional off-chip large bypass capacitors. The prototype ABC fabricated in a 0.25 um CMOS occupies the active die area of 2.25 $\textrm{mm}^2$ and shows the measured DNL and INL of maximum 0.43 LSB and 0.82 LSB, respectively. The ADC maintains the SNDR of 43 dB and 41 dB up to the 110 MHz input at 200 MS/s and 220 MS/s, respectively, while the SNDR at the 500 MHz input is degraded as much as only 3 dB than the SNDR at the 110 MHz input.