• 한국산림과학회지
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• 제97권2호
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• pp.144-151
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• 2008

Using 8 isozyme polymorphic loci as gene markers, we studied the spatial distribution of genotypes in a naturally regenerated uneven-aged Eastern Siberian Fir (Abies nephrolepis Max.) stand (1ha, $100{\times}100m$) on Mt. Ohdae in northeastern South Korea. Gregorius' distograms and Moran's I correlograms revealed no evidence of significant genetic structure at three spatial classes of 5 m, 10 m, and 20 m. Extensive gene flow, due to the long distance dispersal of pollen and seeds in A. nephrolepis, may account for the lack of fine-scale spatial structure. Alternatives would be overlapping seed shadows caused by high densities of A. nephrolepis adult trees (160 trees/ha) and/or intraspecific competition resulting in extensive thinning within maternal half-sib groups.

• Journal of Ecology and Environment
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• 제37권1호
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• pp.21-29
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• 2014

Robinia pseudoacacia has become invasively naturalized in Japan. We investigated the role of sexual reproduction in the development of R. pseudoacacia riparian forests along the Chikumagawa River in Japan, by using five chloroplast (cpSSR) and seven nuclear (nSSR) markers. We identified eight chloroplast haplotypes and 147 nuclear genotypes from 619 R. pseudoacacia trees sampled in three plots (Plots A, B, and C) and along two line transects (Lines D and E). CpSSR analyses showed that multiple maternal lines were distributed along the river, and that some haplotypes from different populations overlapped. In addition, while Plots A and B were separated by a short distance, only these two plots exhibited genetic differentiation in the haplotypes. In the nSSR analysis, all pairwise $F_{ST}$ values among the three plots were significantly different from zero. Kinship analysis based on nSSR markers revealed that kinship connected many individuals to another individual from the same plot. These results indicate that seed dispersal near to mother trees contributes to the fine-scale genetic structure of R. pseudoacacia riparian forests. Our results indicate that sexual reproduction, in addition to asexual reproduction, is a major contributor to the fine-scale formation of R. pseudoacacia forests.

• 한국자원식물학회지
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• 제20권6호
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• pp.530-539
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• 2007

Genetic variation and structure of 9 subpopulations of Symplocarpus renifolius Schott ex Tzvelev on Mt. Cheonma, in Korea, were determined via starch-gel electrophoresis. The genetic diversity at 10 loci for 8 isozymes ($P_{99}=66%,\;A=2.26,\;H_o=0.212,\;H_e=0.230$) was found to be considerably higher than that seen in other long-lived perennial plants. On the whole, the genotype frequencies were in accordance with Hardy-Weinberg expectations. Approximately 5%($\theta=0.049$) of the total variability was among subpopulations. The high levels of observed genetic diversity in S. renifolius were attributed to a universal outcrossing system and other specific factors like differences in age classes and widely scattered individuals around the main distribution. Heterozygosity was highest at a mid-range of elevation($450m{\sim}600m$). The lowest heterozygosity at lower elevation was attributed to the possible origin of seeds transported by water from upstream regions during the monsoon season. Spatial structure in a subpopulation evidenced a strong autocorrelation between closer individuals within $3{\sim}4m$ of distance. This was assumed to be attributable to the restricted seed dispersal characteristics of S. renifolius. In accordance with the findings generated in this study, some implications regarding the conservation of S. renifolius at the Mt. Cheonma were also presented.

• The Korean Journal of Ecology
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• 제15권3호
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• pp.209-220
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• 1992

We investigated major plant communities and seed dispersal patterns in areas a(alt. 2,263 ~2,350m),b(alt. 2,350~2,420m), c(alt. 2,420m) with levels of a crater on mt. paektu. Thirty two species distributed around chunji consisted of 24 perennial herbs, 1 biennial herb and 7 alpine dwarf shurbs. Their fruit types were quite diverse; capsule(11), achene(10), grain(4), legume(2), follicle(2), schizocarp(2) and drupe(1). in area a, adjancent to the chunji(lake) water surface, gramineae sp., cyperaceae sp., chrysanthemum zawadskii and bistorta ochotensis were occured as members of the major plant communities. Species of gramineae and cyperaceae seem to have become dominant because their seeds float well. Astragalus membranaceus is legume and endures the winter cold and summer drought. in area c, alpine dwarf shrubs (e. g.rhododendron redowskianum, rhododendron aureum, empetrum nigrum) were dominant. In area c, compared to a and b, the weather is drier because of strong wind and lower water holding capacity of the soil. Therefore the pattern of plants distributing around chunji is different from that in western slope on mt.paektu. environmental factor such as wind, temperature and water may contribute to the pattern.

• 식물병연구
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• 제30권1호
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• pp.50-59
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• 2024

Charcoal rot disease, caused by the fungus Macrophomina phaseolina, is one of the most important diseases of Sesame (Sesamum indicum) all over the world. However, the population biology of M. phaseolina is poorly understood. In this study, M. phaseolina isolates from five different regions of Iran (Khuzestan, Fars, Bushehr, Hormozgan, and Kohgiluyeh & Boyer-Ahmad provinces) (n=200) were analyzed for genetic variation using inter simple sequence repeats marker. In total, 152 unique haplotypes were identified among the 200 M. phaseolina isolates, and gene diversity (H=0.46-0.84) and genotypic diversity were high in each of the regions. The structure analysis clustered five Iranian populations into two distinct groups, the individuals from group 1 were assigned to the Bushehr population and the individuals from Khuzestan, Fars, Hormozgan and Kohgiluyeh & Boyer-Ahmad were aggregated and formed group 2. The results matched with genetic differentiation and gene flow among regions. Analyses of the distribution of gene diversity within and among five Iranian populations were 61% and 39%, respectively. Our results showed that infected seeds are thought to be the dominant mechanism responsible for the spreading of the pathogen in southern parts of Iran. In summary, it is essential to have local quarantine and prevent seed exchanges between geographical populations to restrict the dispersal of pathogen over long distances and provide certified seeds in Iran.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권4호
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• pp.229-234
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• 2021

Iris dichotoma Pall. found on Daechung Island in Korea has been designated as an endangered species. To aid in conservation efforts of this species, this study investigated its germination characteristics and seed dormancy type. Four sets of seeds were incubated at different temperatures (4/1℃, 15/6℃, 20/10℃, and 25/15℃). One set of seeds was cold stratified (4 weeks at 4/1℃). The final germination rate and mean germination time showed that the optimal germination temperature was 25/15℃. Final germination rates were ~70%, showing no significant difference among temperature treatments. However, mean germination time were significantly different among all temperature treatments except for 4/1℃. Mean germination time for seeds with temperature treatments of 15/6℃, 20/10℃, and 25/15℃ were 3.2, 2.1, and 1.5 weeks, respectively. At 25/15℃, the mean germination time was half of that at 15/6℃. Seeds of I. dichotoma had fully developed embryos at the time of dispersal. No additional growth of the embryo was observed. Cold stratification did not affect the final germination rate or the mean germination time. This study shows that seeds of I. dichotoma have no physiological or morphological dormancy, unlike other members of the Iris genus known to have seed dormancy that needs a relatively high incubation temperature (≥25/15℃) for mass propagation to occur. These results will be useful for understanding ecophysiological mechanisms related to the species' habitat. They are also useful for mass propagation of I. dichotoma for the purpose of conserving this endangered species.

• 한국산림과학회지
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• 제90권4호
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• pp.565-572
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• 2001

본 연구는 오대산의 전나무 노령임분(老齡林分)내 숲틈에서 발생된 1~2년생 전나무 치수(416개체)의 공간적 유전구조를 파악하기 위하여 ISSR(inter-simple sequence repeats) 표지자 분석을 실시하였다. 대상 숲틈의 크기는 $1,500m^2(50m{\times}30m)$로 전나무이외 수종의 상층임관 일부와 중 하층임관이 제거되고, 전나무 성목은 입목고사(立木枯死) 혹은 수세가 불량한 상태이다. 31개의 다형성 ISSR 표지자를 이용한 공간의 자기상관성분석에서는 15.6m이내에 유전적 동질성을 갖으며, 이후 31.2m까지는 임의분포를 나타내었다. 숲틈내 전나무 성목의 평균수고(21.1m), 종자의 산포범위, 성목간 평균거리(23.7m)를 고려할 때, 전나무 치수의 유전적 군락 크기(genetic patch size)는 모수의 분포밀도에 따라서 제한받는 것으로 추정된다. 치수 산포에 대한 방향성 파악을 위하여 유전적 거리를 이용한 다차원척도법의 형상좌표를 '유전적 형상(genetic configuration)'으로 설정하고, 이를 이용한 분산도분석을 실시하였다. 지향성 분산도에서는 동서방향으로 거리의 증가에 따라 치수간 유전적 동질성이 계속 감소하는 것으로 나타났다. 오대산 전나무림의 막대한 종자생산량과 조사구내 치수 발생수의 임의분포와 임상(林床)의 균일성을 고려하면, 이러한 전나무 치수의 유전적 방향은 모수간 충실율 차이나 국소환경보다는 종자 산포의 방향성에 따른 것으로 생각된다.

• 한국환경생태학회지
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• 제30권4호
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• pp.658-675
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• 2016

분포지의 환경특성, 개체군구조, 생태적지위, 생활사 특성 그리고 개체군 통계적 연구는 효과적인 보전을 위해 필수적인 정보이다. 우리는 12년간의 분포조사를 통해 총 37개의 분포지를 확인하였고 그중 12개 지역에 있는 25개의 집단을 대상으로 생육지의 환경과 개체군 구조를 조사하였다. 강원도 평창군 대관령면의 8개 집단을 대상으로 4년간에 걸친 개체군동태를 조사하였다. 4년간의 재배실험과 화분매개곤충의 영향을 관찰하였다. 조사결과 산작약은 비교적 넓은 식생환경에 걸쳐 분포하였다. 조사가 이루어진 25개 집단의 개체군 구조는 비교적 안정적인 집단과 극단적으로 성체만이 존재하는 집단 등 상당한 차이를 나타내었다. 조사기간 동안 상층식생에 생성된 숲 틈이 개체군 동태에 영향을 미치는 요인으로는 추정되었다. 재배실험에서 유묘는 토양의 유기물이 상대적으로 적은 곳에서 잘 성장하였다. 화분매개곤충의 방문이 결실한 종자의 수와 결실률에서 높게 나타나는 경향이 있었다. 결론적으로, 산작약의 분포는 환경적 요인과 더불어 분포에 관여하는 다른 요인의 연구가 필요하며 그 중에서 종자의 운반체에 대한 연구의 필요성을 제안하였다. 현재의 산작약 개체군은 과거 급격한 분포지의 감소 이후 확대되는 과정으로 평가되었다. 멸종위기야생식물인 산작약의 효과적인 보전을 위해서는 시공간적 확장을 통한 종에 대한 이해와 이를 바탕으로 한 생태계의 건강성 유지를 제안하였다.

• 한국환경생태학회지
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• 제36권1호
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• pp.87-101
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• 2022

난온대 상록활엽수림의 변화과정을 장기 모니터링하기 위해 1996년 완도수목원에 설치된 16개 고정조사구(시험구, 대조구)를 대상으로 식생구조 변화를 분석했다. 특히 1996년도 복원기법으로 도입한 시험처리(솎아베기, 식물도입)가 식생 발달에 미친 영향을 심도 있게 논의했다. 교목층에 붉가시나무가 우점한 군락(고정조사구 1~3)에서는 이 종의 평균상대우점치(MIP)가 줄고, 외부에서 들어온 상록활엽수종의 MIP가 늘어나 장래 식생구조 변화가 예상된다. 붉가시나무-낙엽활엽수림(고정조사구 5, 7), 소나무림(고정조사구 9, 10)에서도 상록활엽수종의 세력 확장이 확인됐다. 솎아베기를 시행했던 시험구에서 조류 피식 산포형인 생달나무, 참식나무, 후박나무 등의 종 유입과 상록활엽수종의 세력 확장이 두드러져, 난온대림 식생 발달을 촉진하는데 유효했다고 본다. 시험구에 상록활엽수종을 심어 식생구조 변화를 꾀했지만, 식재 수량이 적어 식생구조 변화에 끼친 영향이 미약했던 것으로 보인다. 다른 식생유형보다 소나무림은 소나무, 곰솔의 쇠퇴에 따른 상록활엽수림으로의 식생구조 변화가 뚜렷했다.

• Journal of Ecology and Environment
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• 제35권1호
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• pp.27-34
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• 2012

Typha angustifolia has ecological characteristics of clonal growth similar to Phragmites australis. The plant spreads byclonal growth and seed dispersal. In this study, for the three stands which have different settlement age at the Baksilji wetland in Korea, genetic diversity was estimated by random amplification of polymorphic DNA analysis to evaluate the change in genetic diversity of T. angustifolia during stand development in the same population. Stand (ST) 1 was the oldest and ST 4 was the youngest. ST 5 was in a small ditch out of the Baksilji. Although the ST 1, ST 2, and ST 3 did not differ significantly in vegetational or physical environment, the genetic diversity estimated according to Nei's gene diversity (h) and the Shannon index (i) increased in the order of ST 1 < ST 2 < ST 3 contrary to formative age. The genetic diversity of ST 4 was much higher than that of the other three stands. ST 4 has similar abiotic environmental conditions with slight T. angustifolia dominance, and seems to be in the early establishment stage. ST 5 differed from the other stands in vegetational and soil environments, which can result in stressful cattail conditions. Even though the ST 5 stand was not younger than the ST 4 stand, ST 5 showed the highest genetic diversity. Our results indicate that after early settlement of the T. angustifolia population, genetic diversity within the species decreased over time and that the decreasing pattern of genetic diversity within T. angustifolia stands is not likely to occur under stressful conditions.