• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1101-1106
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• 2009

Multivalent and multi-specific antibodies can provide valuable tools for bio-medical research, diagnosis and therapy. In antigen-antibody interactions, the avidity of antibodies depends on the affinity and the number of binding sites.$^1$ As artificial multivalent antibody agents, single chain Fv-streptavidin fusion tetramer proteins $(scFv-SA)_4$ have been previously tested.$^{1,\;2}$ Although, the Fab domain is known to be more stable than scFv in animal models,$^{3,\;4}$ it has never been used to make a multivalent agent with a streptavidin fusion. In this study, we prepared tetra-valent $(Fab-cSA)_4$ by fusing Fab with core streptavidin (cSA). This molecule was made using inclusion body production, refolding and chromatography purification. Affinities of the Fab-cSA tetramer and a scFv-cSA tetramer to a cell surface antigen were compared by ELISA using biotin-HRP. The Fab-cSA tetramer showed higher binding avidity than the scFv-cSA tetramer. The higher binding avidity of the Fab-cSA tetramer demonstrates its potential as a therapeutic agent for target-specific antibody therapy.

• The Journal of Korean Medicine
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• v.29 no.1
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• pp.156-166
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• 2008

Objective : Pamooltang (PM) and Sipjeondaebotang (SC) are used in Korea to treat many diseases such as sterility, menstrual disorder, and general prostration. We made a comparative study of PM and SC which are different in component ratio between Astragalus membranaceus BUNGE (AC) and Cinnamomum cassia PRESL. (CC). Methods : Anti-oxidation was studied by 1.1.-diphenyl-2-picrylhydrazyl (DPPH) assay and anti-inflammation was investigated by prostaglandin $E_2\;(PGE_2)$ and nitric oxide (NO) inhibition assay. For immune response activities, this study used NO synthesis on RAW 264.7 cells and splenocyte proliferation. Results : The results showed that PM and SC components had no significant effect of anti-oxidation or anti-inflammation. However, we observed their effects upon inducible NO synthesis in Raw 264.7 cells. The SC2 stimulated NO synthesis $11.42\pm1.36{\mu}M$ (control; $0.89\pm0.00{\mu}M$). PM and SC components had the effect of immune response which in a dose-dependent manner significantly induced the splenocyte proliferation. The splenocyte proliferation induced by SC2 was higher than others at the concentration of 1, 10, 100, 1000 and 2000 ${\mu}g/ml$. The SC8 was shown to up-regulate IgG, 100 ${\mu}g/ml$ 3.3 times, 1000 ${\mu}g/ml$ 2.6 times as a control. Conclusions : These results may have important implications for our understanding of the ratios of AC and CC in SC.

• Asian Journal of Turfgrass Science
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• v.19 no.1
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• pp.17-25
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• 2005

Pathogenicity of entomopathogenic nematde, Steinernema carpocapsae Pocheon strain (ScP) was evaluated against different larval stages(2nd, 3-4th and 5th) of Anomis commode and Anomis mesogona(Lepidoptera: Noctuidae) in petri dish and pot. The $LC_{50}$ values were increased in proportion to larval stage of A. commoda and A. mesogona. $LC_{50}$ value of ScP against 2nd instar of A. commoda and A. mesogona was 9.7 and 4.5, respectively. The 2nd instar of both species was also susceptible to ScP in pot test. That is, mortality of 2nd instar of A. commode and A. mesogona was higher representing $72.5\%\;and\;87.5\%$ 5 days later after treatment, respectively, when ScP was applied at the ,ate of 90,000 infective juveniles(Ijs) per $pot(=1\times10^9\;Ijs/ha)$. However, susceptibility was decreased from 3rd instar. Mortality of 5th instar of A. commoda and A. mesogona was $5.0\%\;and\;10.0\%$, respectively, at the rate of 90,000 Ijs/pot. When ScP was applied into pot including mixed larval stages from 2nd to 5th instar(10 larvae far 2nd instar, 10 larvae for 3rd - 4th instars, and 10 larvae for 5th instar) at the rate of 90,000 Ijs/pot, mortality of A. commoda and A. mesogona was $69.2\%\;and\;50.0\%$, respectively.

• Journal of the Korea institute for structural maintenance and inspection
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• v.10 no.1
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• pp.197-204
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• 2006

This experimental study of prominent section bending behavior beam without shear connector provides bond capacity between concrete profile and initial stiffness of SC beam by comparing the test result with a theological analysis result and an ANSYS(common structural analysis program) analysis result. The compared result provides a fundamental study for practical use of efficient SC beam. Test result indicates 88%-98% rate of theological result in moment capacity and composition ratio shows 30%-70%. In other words, the results are insufficient to make a complete composite action. Therefore, it is need to make pull shear connection of connection method.

• Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.275-281
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• 2012

Yellow croaker Larimichthys polyactis muscle oil was extracted using an environmental friendly solvent, supercritical carbon dioxide (SC-$CO_2$), in a semi-batch flow extraction process. SC-$CO_2$ was applied at temperature $35^{\circ}C$ to $45^{\circ}C$ and $150^{\circ}C$ to $250^{\circ}C$ bar of pressure. The flow rate of $CO_2$ (27.79 g/min) was constant throughout the entire 1.5 h extraction period. The oil extraction yield was influenced by the physical properties of SC-$CO_2$ at different temperatures and pressures. The extracted oil was analyzed by gas chromatography to determine the fatty acid composition. According to our results, the SC-$CO_2$ extracted oil was high in eicosapentaenoic acid and docosahexaenoic acid. In addition, the SC-$CO_2$ extracted oil showed greater stability than n-hexane extracted oil based on the peroxide value and acid value. Thus, the quality of yellow croaker oil obtained by SC-$CO_2$ extraction was slightly higher than that of oil obtained by n-hexane extraction.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.5
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• pp.550-556
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• 2016

Polar codes are one of the most favorable capacity-achieving codes due to their simple structure and low decoding complexity. However, because of the disappointing decoding performance realized using conventional successive cancellation (SC) decoders, polar codes cannot be used directly in practical applications. In contrast to conventional SC decoders, list SC (SCL) decoders with large list sizes (e.g. 32) achieve performances very close to those of maximum-likelihood (ML) decoders. In SCL decoders with large list sizes, however, hardware increase is a severe problem because an SCL decoder with list size L consists of L copies of an SC decoder. In this paper, we present a low-area SCL decoder architecture that applies the proposed merged processing element-sharing (MPES) algorithm. A merged processing element (MPE) is the basic processing unit in SC decoders, and the required number of MPEs is L(N-1) in conventional SCL decoders. Using the proposed algorithm reduces the number of MPEs by about 70% compared with conventional SCL decoders when the list size is larger than 32.

• Journal of the Korean Magnetics Society
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• v.17 no.4
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• pp.152-155
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• 2007

We have studied electronic structures and magnetic properties of $YMnO_3,\;ScManO_3$ with hexagonal structure using Full Potential Linearized Augmented Plane Wave (FLAPW) method based on LSDA method. LSDA calculation results show that multiferroic $YMnO_3$ shows energy gap due to hexagonal symmetry and magnetic interaction. Because of insulating gap and small Y ion, $YMnO_3$ shows magnetic and ferroelectric state. However, $ScMnO_3$ does not show the energy gap because of strong hybridization of Mn-O for LSDA calculation. We confirmed the stability of multiferroic state for $YMnO_3\;and\;ScManO_3$ using total energy calculations. The antiferromagnetic and ferroelectric states have the lowest energy about 100 meV.

• Journal of Oriental Neuropsychiatry
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• v.20 no.3
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• pp.89-119
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• 2009

Objectives : This study was to investigate the association of Pittsburgh Sleep Quality Index(PSQI), Insomnia Questionaire(IQ), Beck Depression Inventory(BDI) and Diagnos system of oriental medicine(DSOM) in the Insomnia Patients. Methods : For this study, we carried out PSQI, IQ, BDI and DSOM of 37 patients with insomnia who have come to Donguei oriental hospital of Donguei university from November 2008 to May 2009. And Using cross tabulation analysis, verified the association of PSQI, IQ, BDI and DSOM. Results : 1. The most Frequent Pathogenic Factor is blood-deficiency(血虛) in total patients. 2. The score of BDI has positive correlation with sc10 of deficiency of qi(氣虛), damp(濕), kdney(腎) in total patients. 3. The score of PSQI has positive correlation with zp of liver(肝) in total patients. 4. The score of PSQI has positive correlation with sc10 and zp of insufficiency of Yin(陰虛), and the score of BDI has positive correlation with sc10 of blood stasis(血瘀) and zp of liver(肝) in female patients. 5. The score of PSQI has positive correlation with sc10 of deficiency of qi(氣虛) and zp of heart(心), and the score of IQ has positive correlation with sc10 of liver(肝) in male patients. 6. The duration from onset in the group of 22-59years are longer than the group of 60-80 years and the duration of using hypnotics have positive correlation with total sleep time in 22-59years group. 7. The score of PSQI has positive correlation with zp of liver(肝) in 22-59years group. 8. The score of PSQI has positive correlation with the score of BDI in 60-80years group. 9. The score of BDI has positive correlation with sc10 of deficiency of qi(氣虛) and zp of blood-deficiency(血虛), and the score of IQ has positive correlation with zp of coldness(寒) in 60-80years group. 10. The score of IQ has positive correlation with sc10 and zp of dryness(燥) in below 6 Months Group. 11. The score of PSQI has positive correlation with the score of BDI in over 6 Months Group. 12. The score of PSQI has positive correlation with zp of liver(肝) in over 6 Months Group. 13. The score of IQ has positive correlation with sc10 of dryness(燥) in BDI 2nd Grade Group. 14. The score of BDI has positive correlation with sc10 of kidney(腎) and the age has positive correlation with zp of heart(心) in BDI 3rd Grade Group. 15. The age has positive correlation with sc10 of damp(濕) in BDI 4th Grade Group. Conclusions : This study provides insights into the complicated associations of the pattern of insomnia with depression and Diagnos system of oriental medicine. And especially this study showed apparent correlation between insomnia and depression in 60-80years group and over 6 months group.

• The Korean Journal of Food And Nutrition
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• v.24 no.2
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• pp.233-238
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• 2011

Smilax china L. rhizome extract(SCE), called 'Tobokreung' in Korean traditional medicine was investigated the influence on heavy metal contents(particularly Pb, Cd, As, and Hg) in Sprague-Dawley rats for 3 weeks. The test groups were divided into 4 group, normal, control, and SCE feeding groups, SC1(13 mg/kg) and SC2(26 mg/kg), respectively. The three group except normal group, were fed heavy metal such as Pb, Cd, As, and Hg. Body weight gain and the weight of target organs (liver and kidney) were determined and had not shown significant differences. Pb, Cd, and As contents in the kidney of SCE feeding groups were tended to decrease after 3 weeks, and SC2 group showed remarkably decrease of them. In the liver, the 3 heavy metal contents except Cd of SC2 group, were decreased rather than that of control group. Pb contents in the serum and the hair of the SC2 group showed significantly decreasing. All the taken together, we investigated the effect of SCE on the 4 heavy metal contents in rats for 3 weeks, and found out that more dosage of SCE made lower heavy metal contents in vivo, for the first time.

• Food Science of Animal Resources
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• v.29 no.3
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• pp.374-381
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• 2009

Low-fat pork sausages (LFPS) were prepared with 1% transglutaminase (TG) and 0.5% sodium caseinate (SC), and with or without different type of hydrocolloids (0.3%; acorn, AC or mungbean, MB) to evaluate the effects of these ingredients on the physicochemical and textural properties of LFPS with reduced salt. pH, moisture content (%) and lightness of low-fat/salt pork sausages (LFSPS) were affected by the addition of TG combined with SC (TG-SC) and acorn or mungbean powders affected the lightness and yellowness of LFSPS. However, cooking yield of LFSPS decreased, while textural properties were increased with the addition of TG-SC combination, which did not affect expressible moisture contents (%) of LFSPS. Both AC and MB tended to improve the cooking yield and water holding capacity of LFSPS, especially, MB rather than AC. However, these had no effect on the textural properties of LFSPS, except for textural chewiness. These results indicated that AC and MB powders could be used as a water binding agent in TG-SC combination of LFSPS.