• Title/Summary/Keyword: SALT STRESS

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Prevalence of surgical affections with their clinical management in crocodiles (Crocodylus porosus) in Bangladesh

  • Arif, Abu S.M.;Hashim, M.A.;Runa, R.A.;Chowdhury, E.H.;Rahman, Md Siddiqur;Song, Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.33 no.3
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    • pp.313-317
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    • 2010
  • This study was conducted in the saltwater crocodile (Crocodylus porosus) for the occurrence of various surgical affections with their clinical management. During the period of January 2006 to June 2008, a total of 68 adult crocodiles were considered as reference population from the Reptiles Farm Ltd. at Bhaluka, Mymensingh. The occurrence of surgical affections of adult female (88.9%) was significantly (P<0.01) higher than male (11.1%). Prevalence of common surgical affections were wound: 72.2%, fibroma: 11.1% abscess: 5.6%, fracture: 5.6%, tail necrosis: 2.8%, myiasis: 2.8%. Wound was the highest among the affections. Among the identified wounds, biting wound was higher. In summer (57.7%) the occurrence of wound was higher compared to other seasons. In winter season (7.7%) the occurrence of abscess, myiasis, fibroma were higher compared to other seasons. Wounds and fibroma are the major surgical affections in salt water crocodile. The most common site of surgical affection was recorded in crocodile's tail (30.6%). It was suggested that minimal stress, allowing constant and easy access to water, removal of roots of the tree in the basking land of crocodiles pen, basking land of the pen is better filling up by sand, clinical surgical managements are essential for better health and production.

Development of the Booklet ″Prevention and Management of Hypertension for Older Adults″

  • Kim, Kyungwon;Hyunjoo Kang;Eunmi Shin;Kim, Hee-Seon
    • Journal of Community Nutrition
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    • v.6 no.1
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    • pp.26-34
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    • 2004
  • Hypertension is prevalent among older adults, and nutrition is important for hypertension management. However, there are few nutrition education materials developed for older adults. The purpose of this study was to develop a booklet for prevention and management of hypertension for older adults. Based on lesson plans, topics for the booklet included introduction to hypertension, hypertension prevention through weight management, nutritional management of hypertension, and lifestyle modifications. After several revisions of the draft were made, illustrations and icons appropriate to the text were designed by a graphic designer using Illustrator 9.0 and Photoshop 6.0. The booklet consisted of four chapters and 40 pages. The first chapter dealt with information on hypertension, risk factors, complications and dietary guidelines. These were mainly explained by illustrations and characters of older adults. The second chapter included assessment of obesity, reducing fat intake and behavioral change strategies. The third chapter focused on practical tips for reducing salt, eating more fiber and calcium, and sample menus. The fourth chapter presented information for quitting smoking and drinking, and stress management. Finally, games, meal planning and quizes, were presented as reinforcement. Based on pilot testing with 10 adults aged 50 and older, minor changes were made in wording, expressions and information. This booklet is characterized by using simple and specific messages, providing tips for dietary and lifestyle changes, and using illustrations and characters of older adults to increase understanding. The revised booklet is self-explanatory and can be used by older adults or in nutrition education for older adults.

Measuring Leaf Areas with a Structured-Light 3D Scanner (3차원 구조광 스캐너를 이용한 식물의 잎 면적 측정 방법)

  • Nam, Kyong-Hee;Ko, Eun Mi;Mun, Saeromi;Kim, Chang-Gi
    • Korean Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.232-238
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    • 2014
  • We have developed a non-destructive, touch-free method for estimating leaf areas with a structured-light three-dimensional (3D) scanner. When the surfaces of soybean leaves were analyzed with both the 3D scanner and a leaf area meter, the results were linearly related ($R^2=0.90$). The strong correlation ($R^2=0.98$) was calculated between shoot fresh weights and leaf areas when the scanner was employed during growth stages V1 to V4. We also found that leaf areas measured by the scanner could be used to detect changes in growth responses to abiotic stress. Whereas under control conditions the areas increased over time, salt and drought treatments were associated with reductions in those values after 14 d and 12 d, respectively. Based on our findings, we propose that a structured-light 3D scanner can be used to obtain reliable estimates of leaf area and plant biomass.

Antioxidant and Neuroprotective Effects of Doenjang Prepared with Rhizopus, Pichia, and Bacillus

  • Kang, Su Jin;Seo, Ji Yeon;Cho, Kye Man;Lee, Chang Kwon;Kim, Jeong Hwan;Kim, Jong-Sang
    • Preventive Nutrition and Food Science
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    • v.21 no.3
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    • pp.221-226
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    • 2016
  • A new type of doenjang was manufactured by mixing soaked soybean, koji (Rhizopus oryzae), cheonggukjang (Bacillus amyloliquefaciens MJ1-4 and B. amyloliquefaciens EMD17), and Pichia farinosa SY80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with R. oryzae. The mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. The extract of mixed culture doenjang showed higher antioxidant activity than the commercial doenjang as evaluated by the ferric reducing antioxidant power assay although it was not significantly different from the commercial product in 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities. Further, the mixed culture doenjang reduced intracellular reactive oxygen species levels and protected cells from glutamate-induced cytotoxicity more efficiently in human hippocampal HT22 neuroblastoma cells than the commercial doenjang. In conclusion, a newly-developed mixed culture doenjang had a strong antioxidant activity in vitro and cultured cell model systems, exhibited a potential to prevent oxidative stress-associated disorders although animal and clinical studies are needed to confirm its in vivo efficacy.

Isolation and Characterization of Glycolate Oxidase Gene from Panax ginseng C. A. Meyer

  • Parvin, Shohana;Pulla, Rama Krishna;Kim, Yu-Jin;Sathiyaraj, Gayathri;Jung, Seok-Kyu;Khorolragchaa, Altanzul;In, Jun-Gyo;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.33 no.4
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    • pp.249-255
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    • 2009
  • The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.

Rheological Properties of Biopolymer Produced by Alkali-Tolerant Bacillus sp. (알카리 내성 Bacillus sp.가 생산하는 생물 고분자의 리올로지적 성질)

  • Lee, Shin-Young;Kim, Jin-Young;Shim, Chang-Sub
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.538-544
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    • 1996
  • A highly viscous biopolymer from alkali-tolerant Bacillus sp. was purified and its rheological properties were studied. 1% (w/v) solution of purified biopolymer showed pseudoplastic fluid behavior with the yield stress similar to those of xanthan and guar gum, and its consistency index was exponentially dependent on concentration and temperature. The concentration dependency of consistency index exhibited two rectilinear plots with different slopes at 1% concentration and pseudoplastic property increased with the increase of biopolymer concentration. The biopolymer solution exhibited a low temperature dependency and the activation energy of flow was 1.16 kacl/g mol. The apparent viscosity was very dependent on the change of pH and the addition of salt. However, no organic solvent effects were observed effects of viscosity synergism with the addition of viscosifier were not observed.

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Cathodic Protection of Reinforced Concrete Slab with Zn-Mesh in Marine Environment (해양환경 중 Zn-mesh를 적용한 콘크리트 슬랩의 음극방식 특성)

  • Kim, Ki-Joon;Jeong, Jin-A;Lee, Woo-Chul
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.1065-1068
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    • 2008
  • Marine bridges are readily deteriorated due to the exposure to marine environment. The concrete deterioration occurred by corrosion of steel in concrete is mainly relevant to chloride in seawater. Chloride ions penetrate through porous concrete, and then reach to the reinforcing steel, and finally corroded them. The corrosion by-products(rusts) increase the volume as much as 6 to 10 times of origin steel. this creates expanding pressure and tensile stress, which cause the structures cracking and spalling. Sometimes the rebar corrosion is accelerated, and then collapsed catastrophically. In order to prevent corrosion damage, it is important to understand well regarding the reason of concrete corrosion, the quantification of its damage, and protection method/system to stop or to mitigate the corrosion. In this study, slab specimens were fabricated to evaluate the effect of cathodic protection which was simulated to marine bridges, and/or port structures. Zn-mesh sacrificial anode has been applied as a chathodic protection system and accelerated test conditions, i.e. temperature and salt concentration have been used in this study.

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Ultrastructural and Histochemical Changes of Mucous Cells in the Gill Epithelium of the Seawater-Adapted Guppy (Poecilia reticulatus) (해수에 적용된 Guppy (Poecilia reticulatus) 아가미 점액세포의 미세구조)

  • 문영화
    • The Korean Journal of Zoology
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    • v.38 no.4
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    • pp.570-579
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    • 1995
  • Ultrastructural and histochemical changes of mucous cells In the freshwater and seawater-adapted guppy (Poecflia reticulatus) gills were observed by the light, scanning-and transmisslon~lectron microscopes. The mucous cells were usually located in the epithelium of primary lameilne projected from the gill arch. The rough endoplasmic retIculum and Golgi complex were hIghly developed In immature mucous cells. The mature mucous cells were nearly filled with the mucous granules. In the freshwater guppy, the histochemical properties of the mucous cells were a mixture of the neutral mucin, sialomucin and sulfomucin. When guppy was adapted to the seawater, the content of acid glycoproteins (slalomucln and sulfomucin) was decreased. In addition, the number of mucous cells in the seawater-adapted group was less than a third of those in the freshwater one. These results suggest that the seawater-adapted guppy would react to the changed osmotic stress of the seawater. And also, the environmental change by the increased salt concentraion might lead to reduce the chance of infections.

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Development of EST-SSRs and Assessment of Genetic Diversity in Little Millet (Panicum sumatrense) Germplasm

  • Ali, Asjad;Choi, Yu-Mi;Hyun, Do-Yoon;Lee, Sukyeung;Kim, Jin-Hee;Oh, Sejong;Lee, Myung Chul
    • Korean Journal of Plant Resources
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    • v.30 no.3
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    • pp.287-297
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    • 2017
  • Little millet (Panicum sumatrense) is well known for its salt and drought stress tolerance and high nutritional value, but very limited knowledge of genetic variation and genomic information is available. In this study, a total of 779 primer pairs were designed from the 22,961 EST sequences of switchgrass (Pancium virgatum), of which 48 EST-SSR markers were developed based on the trials of transferability of these primers in little millet. The EST-SSR amplicons showed reproducible single band polymorphism and produced a total of 160 alleles with an average of 3.3 alleles per locus in 37 accessions of little millet. The average values of expected and observed heterozygosities were 0.266 and 0.123, respectively. The polymorphic information content (PIC) values were observed in range of 0.026 to 0.549 with an average of 0.240. The genetic relatedness among the little millet accessions was evaluated by neighbor-joining dendrogram, which grouped all accessions into two distinct groups. The validation thus demonstrated the utility of the switchgrass EST-SSR markers in assessing genomic relationships in little millet. The findings from this study could be useful for designing strategies for the identification of diverse germplasm for conservation and future molecular breeding programs for little millet.

Study on Antioxidant and Anti-inflammatory Activities of Persicaria tinctoria (쪽의 항산화 및 항염증 활성에 대한 연구)

  • Kim, Soo-Jeung;Jang, Tae Won;Kim, Do-Wan;Park, Jae Ho
    • The Korea Journal of Herbology
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    • v.30 no.6
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    • pp.17-24
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    • 2015
  • Objectives : Persicaria tinctoria belongs to the Polygonaceae family and it has been used as the natural dye traditionally. Also, it is well known that the Persicaria tinctoria is used for treating the following symptoms such as fever, inflammation and edema. The purpose of this study is to investigate the effective source of antioxidants and anti-inflammatory agent from various parts of Persicaria tinctoria.Methods : We investigated the antioxidative and anti-inflammatory properties of the Persicaria tinctoria extracts. Antioxidant activities were measured by 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2, 2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, Fe2+ chelating activity and Reducing power of Persicaria tinctoria extracts. And its inhibitory effect against oxidative DNA damage was evaluated in non-cellular system using φX-174 RF I plasmin DNA. The anti-inflammatory effect of Persicaria tinctoria was measured by using the inhibitory efficacy for the amount of nitric-oxide (NO) produced in LPS induced RAW264.7 cells.Results : The extracts from stem part showed better DPPH scavenging activity compared to those of the leaf and root extracts. Their IC50s were measured as 7.17, 144.40 and 165.07 ug/ml, respectively. These results were similar to that of ABTS radical scavenging assay and reducing power. Also, Persicaria tinctoria showed the protective effects of DNA damage against oxidative stress and anti-inflammatory effect by suppression of NO production in LPS induced RAW264.7 cells.Conclusions : These results showed that various parts of Persicaria tinctoria can be used as an effective source of antioxidants and anti-inflammatory agents via antioxidative activities and anti-inflammatory effect.