Browse > Article
http://dx.doi.org/10.5142/JGR.2009.33.4.249

Isolation and Characterization of Glycolate Oxidase Gene from Panax ginseng C. A. Meyer  

Parvin, Shohana (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Pulla, Rama Krishna (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yu-Jin (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Sathiyaraj, Gayathri (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Jung, Seok-Kyu (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Khorolragchaa, Altanzul (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
In, Jun-Gyo (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.33, no.4, 2009 , pp. 249-255 More about this Journal
Abstract
The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.
Keywords
abiotic stress; glycolate oxidase; gene expression; real-time PCR;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Verslues PE, Kim, YS, Zhu JK. Altered ABA, proline and hydrogen peroxide in an Arabidopsis glutamate: glyoxylate aminotransferase mutant. Plant Molecular Biology 64: 205- 217 (2007).   DOI
2 Somerville CR. An early Arabidopsis demonstration: resolving a few issues concerning photorespiration. Plant Physiology 125: 20-24 (2001).   DOI   ScienceOn
3 Ow DW, Wood KV, DeLuca M, DeWet JR, Helinski DR, Howell SH. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856-859 (1986).   DOI   ScienceOn
4 Borst P. Peroxisome biogenesis revisited. Biochim Biophys Acta 1008: 1-13 (1989).
5 Macheroux P, Massey V, Thiele DJ. Expression of spinach glycolate oxidase in Saccharomyces cerevisiae: Purification and characterization. Biochemistry 30: 4612-4619 (1991).   DOI   ScienceOn
6 Sharkey TD. Estimating the rate of photorespiration in leaves. Physiologia Plantaurm 73: 147-152 (1988).   DOI
7 Boldt R, Edner C, Kolukisaoglu U, Hagemann M, Weckwerth W, Wienkoop S, Morgenthal K and Bauwe H. D-Glycerate 3-kinase, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family. Plant Cell 17: 2413-2420 (2005).   DOI   ScienceOn
8 Keys AJ. The re-assimilation of ammonia produced by photorespiration and the nitrogen economy of C3 higher plants. Photosynthesis Research 87: 165-175 (2006).   DOI
9 Rachmilevitch S, Cousins AB and Bloom AJ. Nitrate assimilation in plant shoots depends on photorespiration. Proc Natl Acad Sci USA 101: 11506-11510 (2004).   DOI   ScienceOn
10 Moreno JI, Martin R, Castresana C. Arabidopsis SHMT1, a serine hydroxyl methyltransferase that functions in the photo respiratory pathway influences resistance to biotic and abiotic stress. Plant Journal 41: 451-463 (2005).   DOI   ScienceOn
11 Verniquet F, Gaillard J, Neuburger M, Douce R. Rapid inactivation of plant aconitase by hydrogen peroxide. Biochem J. 276: 643-648 (1991).
12 Holger F, Telma ES, Estela MV, Ulf-Ingo F, Veronica GM. Generation of hydrogen peroxide in chloroplasts of Arabidopsis overexpressing glycolate oxidase as an inducible system to study oxidative stress. Plant Physiology 148: 719-729 (2008).   DOI   ScienceOn
13 Simon B, Ali N, Yair H, Micha V. Transcriptional and posttranscriptional regulation of the glycolate oxidase gene in tobacco seedlings. Plant Molecular Biology 45: 399-407 (2001).   DOI   ScienceOn
14 Goyal A. Effects of water stress on glycolate metabolism in the leaves of rice seedlings (Oryza sativa). Physiol Plantarum 69: 289-294 (1987).   DOI
15 Kim JH and Nah SY. Effects of ginsenoside $Rg_3$ epimers on swine coronary artery contractions. J Ginseng Res. 29(3): 119-125 (2005).   DOI   ScienceOn
16 Kook S, Han HK, Kim GH, Choi K. The anti-hepatotoxic effect of ginseng in rats: meta–-analysis. J Ginseng Res. 32(2): 161-170 (2008).   DOI   ScienceOn
17 Parvin S, Pulla RK, Shim JS, Kim YJ, Jung DY, Yang DC. Isolation and Characterization of Cinnamoyl-CoA Reductase Gene from Panax ginseng C. A. Meyer. J Ginseng Res. 32(3): 232-237 (2008).   DOI   ScienceOn
18 Morris PC, Kumar A, Bowles DJ, Cuming AC. Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur J Biochem. 190: 625-30(1990).   DOI   ScienceOn
19 Gould SJ, Keller GA, Subramani S. Identification of a peroxisomal targeting signal at the carboxy terminus of firefly luciferase. J Cell Biol. 105: 2923-2931 (1987).   DOI
20 Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 157: 105-32 (1982).   DOI
21 Smith SM, Leaver CJ. Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination. Plant Physiol. 81: 762-767 (1986).   DOI   ScienceOn
22 Kindl H. 3-Oxidation of fatty acids by specific organelles. In: Stumpf PK, Conn EE (ed), The Biochemistry of Plants, Academic Press, London 31-52 (1987).
23 Kim YJ, Ham AR, Shim JS, Lee JH, Jung DY, In JG, Lee BS, Yang DC. Isolation and characterization of trepene synthase gene from Panax ginseng. J Ginseng Res. 32(2): 114-119 (2008).   DOI   ScienceOn
24 Beeching JR, Northcote DH. Nucleic acid (cDNA) and amino acid sequences of isocitrate lyase from castor bean. Plant Mol Biol. 8: 471-475 (1987).   DOI   ScienceOn
25 Greenler JM, Sloan JS, Schwartz BW, Becker WM. Isolation, characterization and sequence analysis of a full length cDNA clone encoding NADH-dependent hydroxypyruvate reductase from cucumber. Plant Mol Biol. 13: 139-150 (1989).   DOI
26 Shin K, Mary AC, Inna A, Davina B, Monica F, Elizabeth John C, Cushman C. Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum. Gene 341: 83-92 (2004).   DOI   ScienceOn
27 Thimm O, Essigmann B, Kloska S, Altmann T, Buckhou TJ. Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis. Plant Physiology 127: 1030-1043 (2001).   DOI
28 Park YS, Jin YH, Kim YC, Choi JD, Cho NJ. Effects of light on spinach glycolate oxidase gene expression. J Biochem Mol Biol. 28: 271-274 (1995).
29 Bertoni GP, Becker WM. Effects of light fluence and wavelength on expression of the gene encoding cucumber hydroxypyruvate reductase. Plant Physiol. 103: 933-941 (1993).   DOI
30 Tchang F, Lecharny A, Mazliak P. Photo stimulation of hydroxypyruvate reductase activity in peroxisomes of Pharbitisnil seedlings. I. Action spectrum. Plant Cell Physiol. 25: 1033-1037 (1984).
31 Mahour K, Saxena PN. Scavenging strategy of Panax ginseng against formed free radicals under stress of mercuric chloride in Ratus novegicus. J Ginseng Res. 32(2): 150-154 (2008).   DOI   ScienceOn