• 제목/요약/키워드: S180

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Antitumor Effect of Young Radish Kimchi Prepared with Young Radish Cultivated in the Soil Containing Sulfur on Sarcoma-180 Tumor Cells Transplanted Mice (유황처리 열무로 제조한 열무김치의 Sarcoma-180 암세포에 대한 고형암 성장 억제효과)

  • Kong, Chang-Suk;Bak, Soon-Sun;Rhee, Sook-Hee;Kil, Jeung-Ha;Rho, Chi-Woong;Hwang, Hae-Jun;Kim, Nak-Ku;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.10
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    • pp.1520-1524
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    • 2005
  • Young radishes (YR, yeolmu in Korean) were cultivated in the soil with and without sulfur. Young radish kimchi-general (YR kimchi-G) was prepared with YR commonly cultivated in the soil without sulfur. Young radish kimchl-sulfur (YR kimchi-S) and young radish kimchi-sulfur with lime mortar (YR kimchi-SL) were prepared with the YR cultivated in the soil with sulfur an4 sulfur added lime mortar on it respectively. Antitumor effects of methanol extracts from the YR kimchis were investigated in sarcoma-180 tumor cell transplanted mice. The solid tumor growth was significantly inhibited by the YR kimchi-SL prepared with YR grown in the soil with sulfur (p < 0.05). The treatment of the methanol extracts from YR kimchi-S and -SL increased the glutathione S-transferase activities and glutathione contents in the livers, compared to thlose of YR kimchi-G and the control. One of the antitumor effects by the YR kimchis was due to the increased the glutathione levels and the glutathione S-transferase activity which is phase 2 enzyme. These results also suggested that the antitumor effects of YR kimchi can be enhanced by using YR cultivated differently in the presence of sulfur that can help to produce sulfur-containing compounds in YR.

Anticancer activity of subfractions containing pure compounds of Chaga mushroom (Inonotus obliquus) extract in human cancer cells and in Balbc/c mice bearing Sarcoma-180 cells

  • Chung, Mi-Ja;Chung, Cha-Kwon;Jeong, Yoon-Hwa;Ham, Seung-Shi
    • Nutrition Research and Practice
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    • v.4 no.3
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    • pp.177-182
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    • 2010
  • The Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat cancers. However, limited information exists on the underlying anticancer effects of the major component of I. obliquus in vivo. We hypothesize that the pure compounds ($3{\beta}$-hydroxy-lanosta-8,24-dien-21-al, inotodiol and lanosterol, respectively) separated from I. obliquus would inhibit tumor growth in Balbc/c mice bearing Sarcoma-180 cells (S-180) in vivo and growth of human carcinoma cells in vitro. To test this hypothesis, the growth inhibition of each subfraction isolated from I. obliquus on human carcinoma cell lines (lung carcinoma A-549 cells, stomach adenocarcinoma AGS cells, breast adenocarcinoma MCF-7 cells, and cervical adenocarcinoma HeLa cells) was tested in vitro. Then, after S-180 implantation, the mice were fed a normal chow supplemented with 0, 0.1 or 0.2 mg of subfraction 1, 2 or 3 per mouse per day. All of the subfractions isolated from I. obliquus showed significant cytotoxic activity against the selected cancer cell lines in vitro. Subfraction 1 was more active than subfraction 2 and subfraction 3 against the A549, AGS and MCF-7 cancer cell lines in vitro. In in vivo results, subfraction 1 isolated from I. obliquus at concentrations of 0.1 and 0.2 mg/mouse per day significantly decreased tumor volume by 23.96% and 33.71%, respectively, as compared with the control. Subfractions 2 and 3 also significantly inhibited tumor growth in mice bearing S-180 as compared with the control mouse tumor. Subfraction 1 isolated from I. obliquus showed greater inhibition of tumor growth than subfractions 2 and 3, which agrees well with the in vitro results. The results suggest that I. obliquus and its compounds in these subfractions isolated from I. obliquus could be used as natural anticancer ingredients in the food and/or pharmaceutical industry.

Medium Improvement for Chlrotetracycline Fermentation

  • 김성웅;유두영
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1975.12a
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    • pp.180.1-180
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    • 1975
  • Streptomyces aureofaciens를 이용하여 chlortetracycline 발효중 생산율을 높이는 방법에 대한 연구가 시행되었다. 지금가지 연구보고된 여러결과와는 달리 2가 금속 이온들은 어느 농도이상이 되면 각각 다른 수준으로 chlortetracycline 생산율을 저하시키는 영향이 있다는 관찰이 되었다.(중략)

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Safety Assessment of Lactiplantibacillus (formerly Lactobacillus) plantarum Q180

  • Kwon, Yoo Jin;Chun, Byung Hee;Jung, Hye Su;Chu, Jaeryang;Joung, Hyunchae;Park, Sung Yurb;Kim, Byoung Kook;Jeon, Che Ok
    • Journal of Microbiology and Biotechnology
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    • v.31 no.10
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    • pp.1420-1429
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    • 2021
  • The safety of the probiotic strain Q180, which exerts postprandial lipid-lowering effects, was bioinformatically and phenotypically evaluated. The genome of strain Q180 was completely sequenced, and single circular chromosome of 3,197,263 bp without any plasmid was generated. Phylogenetic and related analyses using16S rRNA gene and whole-genome sequences revealed that strain Q180 is a member of Lactiplantibacillus (Lp., formerly Lactobacillus) plantarum. Antimicrobial resistance (AMR) genes were bioinformatically analyzed using all Lp. plantarum genomes available in GenBank, which showed that AMR genes are present differently depending on Lp. plantarum strains. Bioinformatic analysis demonstrated that some mobile genetic elements such as prophages and insertion sequences were identified in the genome of strain Q180, but because they did not contain harmful genes such as AMR genes and virulence factor (VF)- and toxin-related genes, it was suggested that there is no transferability of harmful genes. The minimum inhibition concentrations of seven tested antibiotics suggested by the European Food Safety Authority guidelines were slightly lower than or equal to the microbiological cut-off values for Lp. plantarum. Strain Q180 did not show hemolytic and gelatinase activities and biogenic amine-producing ability. Taken together, this study demonstrated the safety of strain Q180 in terms of absence of AMR genes and VF- and toxin-related genes as a probiotic strain.

Effects of Phyllostachyos Folium (PF) on solid tumor in mice (죽엽이 생쥐의 복강암에 미치는 영향)

  • Song, Jin-Soo;Park, Soo-Yeon;Choi, Jeong-Hwa;Kim, Jong-Han
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.22 no.2
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    • pp.39-49
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    • 2009
  • Objective : Phyllostachyos Folium (PF) has been used to treat patients with febrile disease consuming the body fluids marked by fever with restlessness, thirst etc. In the theory of herbology, PF can clear away heat and promote the production of the body fluids, relieve restlessness. Recently PF is known to have anti-bacterial, anti-oxidantic effects. Methods : The present study was carried out to investigate the effects of PF on solid tumor in mice in terms of immune-potentiating and direct cytotoxic action of PF in vitro and vivo study. The present author investigated thymocyte and splenocyte proliferation to confirm immune-potentiating activity of PF and also investigated tumor/body weight ratio and survival rates in tumor bearing mice. Result : In this study, administration of PF decreased tumor/body weight ratio significantly, and prolonged survival duration compared to non-treated control. In addition, treatment with PF suppressed proliferation rate of Sarcoma 180 (S-180) cells significantly, and elevated proliferation rates of thymocytes isolated from normal mice. These results were co-related with in vivo study. Conclusion : In conclusion, these results suggest that PF is useful to treat patient with solid tumor, because PF has direct toxic action for tumor cell and immune -potentiating action for T cells. Further study will need to elucidate exact mechanisms related in anti-cancer action of PF.

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Effects of Iridoid Compounds on RNA and Protein Biosyntheses in Sarcoma 180 cells (Iridoid Compounds가 RNA 및 Protein 생합성에 미치는 영향)

  • Huh, S.O.;Kim, J.H.;Chang, I.M.
    • Korean Journal of Pharmacognosy
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    • v.16 no.2
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    • pp.99-104
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    • 1985
  • To investigate a possible biological activity of iridoid glucosides, six compounds, aucubin, catalpol, gardenoside, geniposide, rehmannioside and swertiamarin, were studied in relation with their potential influences in RNA and protein biosyntheses in murine tumor cell, sarcoma 180, in vitro. Protein biosynthesis was slightly inhibited by aucubin, gardenoside and swertiamarin. Degree of inhibition of RNA biosynthesis by those iridoid appeared to be more sensitive than that of protein biosynthesis. When aucubin was pretreated with ${\beta}-glucosidase$ to produce its genin form and the sarcoma 180 cells were exposed to this aucubigenin, the protein and RNA biosyntheses in the cells were profoundly inhibited. The results indicate that a biologically active from of iridoid compounds is the hydrolytic products of glycoside, i.e. genin form. It is also suggested that sarcoma 180 cells used in the experiments appear to lack of ${\beta}-glucosidase$, since the inhibitory actions of iridoid glucosides were so slight that those glucosides were not hydrolysed by the enzyme to their genin forms.

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Effects of Jeungson-Ojeok-Hwan-Bijukbang on Solid Tumor in Mice (증손오적환(增損五積丸) 비적방(脾積方)이 생쥐의 복강암에 미치는 영향)

  • Lee, Yu-Jin;Kim, Jong-Han;Park, Su-Yeon;Choi, Jeong-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.23 no.1
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    • pp.158-168
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    • 2010
  • Objective : Jeungson-Ojeok-Hwan Bijukbang(JOH) has been used to treat patients with tumor etc. In the theory of Korean medicine, JOH can treat Juk-Chui. JOH is known to have treat Juk-Chui. Juk-Chui is analogous to tumor. Methods : For these reasons, the present study was designed to investigate the effects of JOH on solid tumor in mice in terms of immune-potentiating and direct cytotoxic action of JOH in vitro and vivo study. The present author investigated thymocyte and splenocyte proliferation to confirm immune-potentiating activity of JOH and also investigated tumor/body weight ratio and survival rates in tumor bearing mice. Results : In this study, administration of JOH decreased tumor/body weight ratio significantly, and prolonged survival duration compared to non-treated control. In addition, treatment with JOH suppressed proliferation rate of Sarcoma 180 (S-180) cells significantly, and elevated proliferation rates of thymocytes isolated from normal mice. These results were co-related with in vivo study. Conclusion : these results suggest that JOH is useful to treat patient with solid tumor, because JOH has direct toxic action for tumor cell and immune-potentiating action for T cells. Further study will need to elucidate exact mechanisms related in anti-cancer action of JOH.