• Title/Summary/Keyword: S100B

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Antimutagenic Effects of Browning Products Reacted with Polyphenol Oxidase Extracted from Apple (사과 Polyphenol Oxidase에 의한 효소갈변반응 생성물의 항돌연변이 효과)

  • Baik, Chang-Weon;Ham, Seung-Shi
    • Korean Journal of Food Science and Technology
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    • v.22 no.6
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    • pp.625-631
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    • 1990
  • This study was carried out to investigate the antimutagenic effects of five kinds of apple enzymatic browning reaction products(AEBRP) on mitomycin C (MMC), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide(4NQO), benzo(${alpha}$)pyrene(B(${alpha}$)P) and 3-amino-1,4-dimethyl-5H-pyri-do [4,3-b]indol (Trp-P-1). In spore rec-assay using B. subtilis Hl7($rec^+$) and M45($rec^-$), homocatechol-AEBRP and hydroquinone-AEBRP showed strong antimutagenic effects on MMC and MNNG as the concentration of AEBRP increased. In the Ames test using S. typhimurium TA98 and TA100, hydroxyhydroquinone-AEBRP and pyrogallol-AEBRP showed strong antimutagenic effects on Trp-p-1 and B(${alpha}$)p in TA98 and TA100 in the presence of S-9 mix. Most of AEBRPs suppressed about 50% to 80% the mutagenesis in S. typhimurium TA98 induced by MNNG, however, AEBRPs except hydroxyhydroquinone-AEBRP showed antimutagenic effects of about 94% in TA100. Antimutagenic effects of the five kinds of AEBRPs on 4-NQO were more or less weak, in particular homocatechol-AEBRP exhibited the inhibitory effect of about 48% in TA98, and homocatechol-AEBRP and hydroquinone-AEBRP showed inhibitory effects of about 46% to 58% in TA 100.

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Antimicrobial Activities of Marta Rosemary under Different Processing Conditions (가공조건에 따른 marta rosemary의 항균성)

  • Choi, Hye-Ryun;Son, Sun-Young;Choi, Eon-Ho
    • Korean Journal of Food Science and Technology
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    • v.37 no.1
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    • pp.50-54
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    • 2005
  • Antimicrobial activities of ethanol extracts of marta rosemary (Rosemarinus officinalis L.) against Bacillus subtilis and Salmonella typhimurium were investigated under conditions similar to processing of general food materials to develop natural food preservatives. The strongest antimicrobial activities were shown against the tested organisms at pH 7. In B. subtilis, the herbal extract extended the lag phase at low concentrations, and reduced the specific growth rate during logarithmic phase at high concentrations. In S. typhimurium the herbal extract reduced the specific growth rate even at lower concentrations. These trends were obtained at low pHs of culture solution. Antimicrobial substances of herbs were efficiently extracted with low polar solvents. The thermal stability of antimicrobial substances decreased with heating time at $100^{\circ}C$. S. typhimurium was more sensitive to herbal extracts at $100\;and\;121^{\circ}C$ than B. subtilis.

A Modification of A Microbiological Assay of Vitamin $B_{12}$ In Fermented Vegetables

  • Lim, Sook-Ja
    • Journal of Nutrition and Health
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    • v.15 no.3
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    • pp.202-211
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    • 1982
  • A modification of a microbiological assay of vitamin $B_{12}$ was made and used to determine the vita­min levels during kimchi fermentation. A cyanide-buffer solution of pH 6.0 replaced the metabisufite-buffer specified in the A.O.A.C. method. The vitamin $B_{12}$ activity was decreased by blending kimchi samples for 5 minutes and retained the activity by steaming for 10 minutes before blending. The alkali hydrolysis of kimchi at pH 12.0 for 30 minutes at $121^{\circ}C$ was sufficient to destroy the vitamin $B_{12}$ and permit the detection of analogs with the same assay organism. Vitamin $B_{12}$ reached a maximum of 47ng/100g during the fermentation of kimchi ${15}\;4^{\circ}C.$ Inoculation of the kimchi with Propionibacterium shermanii (ATCC 13673) increased the vitamin production to a maximum of 102ng/100g at 1 week of fermentation. Soy flour (0.5%) or beef extract (0.05%), which were regarded as protein sources, added to the inoculated kimchi further increased the vitamin $B_{12}$ activity to 197 and 203ng/100g.

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High-performance 94 GHz MMIC Low Noise Amplifier using Metamorphic HEMTs (Metamorphic HEMT를 이용한 우수한 성능의 94 GHz MMIC 저잡음 증폭기)

  • Kim, Sung-Chan;An, Dan;Rhee, Jin-Koo
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.8
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    • pp.48-53
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    • 2008
  • In this paper, we developed the MMIC low noise amplifier using 100 nm metamorphic HEMTs technology in combination with coplanar circuit topology for 94 GHz applications. The $100nm\times60{\mu}m$ MHEMT devices for the MMIC LNA exhibited DC characteristics with a drain current density of 655 mA/mm, an extrinsic transconductance of 720 mS/mm. The current gain cutoff frequency $(f_T)$ and maximum oscillation frequency $(f_{max})$ were 195 GHz and 305 GHz, respectively. The realized MMIC LNA represented $S_{21}$ gain of 14.8 dB and noise figure of 4.6 dB at 94 GHz with an over-all chip size of $1.8mm\times1.48mm$.

Appearance Characteristic of Waterbirds in Banseok Ecological Stream (생태하천으로 조성된 반석천의 물새류 출현특성)

  • Park, Seungki;Na, Sangsoo;Park, Daesoon;Han, Jaebong
    • Journal of Korean Society of Rural Planning
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    • v.26 no.1
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    • pp.89-99
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    • 2020
  • This study was conducted as a basic research to create a sound and vital ecological environment in the city compared to the appearance characteristics of Gap stream(G-stream), which are the main stream of Banseok stream(B-stream), by analyzing the characteristics of waterbirds, including Wild Spot-billed Duck (S-Duck), that live in B-stream built as ecological stream. The waterbird survey was conducted by the line census for 3.2km, Jukdong-bridge to Jamiseon-bridge, from January to August 2018. The analysis of the survey was conducted with Relative species density(RD) of the emerging waterbird species, the Species diversity and Density Per Unit area for 100㎡(DPU). The waterbird survey results of B-stream was conducted 65 times. The five types of water birds that appeared during the survey were Spot-billed Duck(Anas poecilorhyncha), Teal(Anas crecca), Little Egret(Egretta garzetta), Great Egret(Egretta alba), and Grey Heron(Ardea cinerea). As a result, for S-Duck at B-stream, RD was 89.9%, monthly species diversity was simple as 0.3801 in January, 0.5943 in February and 0.3501 in August. The DPU of the S-Duck was 0.165/100㎡ in the B-Stream survey section which was 4.9 times higher than the main stream section, G-stream. The non-freezing zone of the city's small stream is expected to play an important role as a winter stop for wild birds such as S-Duck during the freezing period of the huge stream. For this reason, considering the ecological characteristics of wild waterbirds such as S-Duck when creating ecological stream, a: space and linear selection of waterways which can minimize the impact of natural enemy and increasing the number of walkers, b: management water-friendly plants in the low flow channel, c: arrangement walking-bicycle road will be necessary.

Canine nervous-tissue tumors with features of peripheral nerve sheath tumor: histopathological and immunohistochemical findings (말초신경초 종양의 특징을 지닌 개 신경종의 조직병리학적 및 면역조직화학적 진단)

  • Lee, Sun-Gue;Lee, Jae-Ha;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
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    • v.41 no.1
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    • pp.57-61
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    • 2018
  • Canine peripheral nerve sheath tumors (PNSTs) are spindle cell tumors that arise from Schwann cells, perineural cells, fibroblasts or all of them. Based on the morphology and biologic behavior, PNSTs are divided into benign PNST (BPNST) and malignant PNST (MPNST) forms. The aim of this study is to diagnose the two cases of neoplastic tissue samples with features of PNSTs by the histopathology and immunohistochemistry. The study was performed using two specimens from small animal clinic. The first case, A was a mass, 3~4 cm in diameter, extruded from vaginal mucosa of 10-year-old spayed female mixed-breed dog. And the second case, B was a subcutaneous mass, 1.5 cm in diameter, which is originated from right hind leg of 9-year-old castrated male mixed-breed dog. Two cases were stained with hematoxylin and eosin (H&E) for histopathological examination. And also immunohistochemistry (IHC) was performed by the avidin-biotin peroxidase complex (ABC) method with antibodies specific for the following proteins: S-100 protein, smooth muscle actin (SMA) and epidermal growth factor receptor (EGFR). In results, Antoni B schwannoma pattern characterized by pleomorphic, round and fusiform polygonal cells was seen in A. In B, Antoni A pattern, densely packed spindle cells arranged in interlacing bundles was seen in addition to Antoni B pattern. In IHC, cytoplasms of neoplastic cells were diffusely labeled for S-100 expression in A and B. For SMA, both A and B show negative expression. And for EGFR, A shows negative expression but B shows partially positive expression in areas of Antoni B schwannoma pattern. The histopathologic features of two cases coupled with the S-100 immunoreactivity led to a diagnosis of PNST. For SMA, both A and B show negative expression. The diagnosis of A will be a BPNST with the negative result and B will be a MPNST with the positive result for EGFR.

A Range-Scaled 13b 100 MS/s 0.13 um CMOS SHA-Free ADC Based on a Single Reference

  • Hwang, Dong-Hyun;Song, Jung-Eun;Nam, Sang-Pil;Kim, Hyo-Jin;An, Tai-Ji;Kim, Kwang-Soo;Lee, Seung-Hoon
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.13 no.2
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    • pp.98-107
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    • 2013
  • This work describes a 13b 100 MS/s 0.13 um CMOS four-stage pipeline ADC for 3G communication systems. The proposed SHA-free ADC employs a range-scaling technique based on switched-capacitor circuits to properly handle a wide input range of $2V_{P-P}$ using a single on-chip reference of $1V_{P-P}$. The proposed range scaling makes the reference buffers keep a sufficient voltage headroom and doubles the offset tolerance of a latched comparator in the flash ADC1 with a doubled input range. A two-step reference selection technique in the back-end 5b flash ADC reduces both power dissipation and chip area by 50%. The prototype ADC in a 0.13 um CMOS demonstrates the measured differential and integral nonlinearities within 0.57 LSB and 0.99 LSB, respectively. The ADC shows a maximum signal-to-noise-and-distortion ratio of 64.6 dB and a maximum spurious-free dynamic range of 74.0 dB at 100 MS/s, respectively. The ADC with an active die area of 1.2 $mm^2$ consumes 145.6 mW including high-speed reference buffers and 91 mW excluding buffers at 100 MS/s and a 1.3 V supply voltage.

Phylogenetic Relationship and DNA Polymorphism of Boleophthalmus pectinirostris and Scartelaos gigas (Teleostei: Gobiidae) of Korea (한국산 짱뚱어(Boleophthalmus pectinirostris)와 남방짱뚱어(Scartelaos gigas) (Gobiidae)의 분자유전학적 계통연관과 DNA 다형화)

  • Choi, Ki Ho;Chung, Ee Yung;Park, Gab Man
    • Korean Journal of Ichthyology
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    • v.25 no.3
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    • pp.149-156
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    • 2013
  • Phylogenetic relationships and DNA polymorphism among local populations of two Korean gobiidae species: Boleophthalmus pectinirostris and Scartelaos gigas were investigated based on 12S and 16S mitochondrial DNA and mitochondrial cytochrome b DNA sequences. DNA polymorphisms of B. pectinirostris between Suncheon and Gunsan populations were 100% identity from 434 bp segment of 12S rRNA gene and from 444 bp segment of mitochondrial cytochrome b genes, and 99.6% (2 bp different) identity from 484 bp segments of 16S rRNA genes. These results indicated the long period of geographic isolation between two populations of B. pectinirostris in Korea caused such high degrees of DNA polymorphisms. Based on the phylogenetic tree constructed from the two gobiid species in Korea, two genetically distinct groups of B. pectinirostris and S. gigas groups were recognized.

Convective Heat Transfer in a Channel with Isothermal Rectangular Beams (등온사각빔이 부착된 채널에서의 대류열전달)

  • Ree, J.S.;Kwon, S.S.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.7 no.1
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    • pp.63-72
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    • 1995
  • Convective heat transfer in a two-dimensional horizontal and vertical channel with isothermal rectangular beams attached to one adiabatic wall is investigated from the numerical solution of Navier-Stokes and energy equations. The solutions have been obtained for dimensionless beam spacings, S/L=1~4, aspect ratios of beam, H/B=0.25~4, Reynolds numbers, Re=50~1000 and Grashof numbers, $Gr=0{\sim}5{\times}10^4$. The total mean Nusselt number, Nu_T for horizontal and vertical channels shows same value at Gr=0. As Gr increases, Nu_T for horizontal channel increases, but Nu_T for vertical channel shows similar value at S/L=2, H/B=0.25, Re=100. The total mean Nusselt number for horizontal channel is higher than that for vertical channel. As H/B increases, $Nu_T$ for both channel decrease at $Gr=10^4$, Re=100.

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