Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Phylogenetic Relationship and DNA Polymorphism of Boleophthalmus pectinirostris and Scartelaos gigas (Teleostei: Gobiidae) of Korea

한국산 짱뚱어(Boleophthalmus pectinirostris)와 남방짱뚱어(Scartelaos gigas) (Gobiidae)의 분자유전학적 계통연관과 DNA 다형화

  • Choi, Ki Ho (Korea Fisheries Resources Agence West Sea Branch) ;
  • Chung, Ee Yung (Korea Marine Environment & Ecosystem Research Institute, Dive Korea) ;
  • Park, Gab Man (Department of Environmental Medical Biology, Kwandong University College of Medicine)
  • 최기호 (한국수산자원관리공단 서해지사) ;
  • 정의영 (다이브코리아 부설 한국해양환경생태연구소) ;
  • 박갑만 (관동대학교 의학과)
  • Received : 2013.06.10
  • Accepted : 2013.09.10
  • Published : 2013.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Phylogenetic relationships and DNA polymorphism among local populations of two Korean gobiidae species: Boleophthalmus pectinirostris and Scartelaos gigas were investigated based on 12S and 16S mitochondrial DNA and mitochondrial cytochrome b DNA sequences. DNA polymorphisms of B. pectinirostris between Suncheon and Gunsan populations were 100% identity from 434 bp segment of 12S rRNA gene and from 444 bp segment of mitochondrial cytochrome b genes, and 99.6% (2 bp different) identity from 484 bp segments of 16S rRNA genes. These results indicated the long period of geographic isolation between two populations of B. pectinirostris in Korea caused such high degrees of DNA polymorphisms. Based on the phylogenetic tree constructed from the two gobiid species in Korea, two genetically distinct groups of B. pectinirostris and S. gigas groups were recognized.

우리나라 망둑어과 어류 중 말뚝망둥어아과에 속하며 형태학적으로 흡사한 짱뚱어 (B. pectinirostris)와 남방짱뚱어(S. gigas)를 대상으로 12S rRNA, 16S rRNA 및 mitochondria cytochrome b 유전자의 염기서열 분석을 통해, 종내 및 종간의 분자유전학적 특성을 파악하고자 하였다. 짱뚱어 종내 개체간 유전적 차이를 밝히기 위해 mitochondria내에 3가지 유전자를 분석한 결과, 순천지역과 군산지역간의 염기서열은 12S rRNA는 총 434 bp (base pair)의 염기서열을 얻었으며 이를 비교 분석한 결과, 순천지역과 군산지역의 집단 간에는 100% 동일한 염기서열을 보였고, 16S rRNA 유전자는 총 484 bp의 염기서열 중 99.6%의 차이로 단지 2 bp가 치환된 것으로 나타났고, mitochondria cytochrome b 유전자의 경우, 444 bp의 염기서열을 얻었으며 두 지역 간 염기서열은 100%의 일치도를 보였다. 한국산 짱뚱어를 대상으로 한 동일한 종내에서 서식지 (서해, 남해)에 따른 유전적 차이나 지리적 거리의 차이가 있을 것으로 예상하였으나 이상의 3가지 유전자를 비교해 본 결과를 종합해 보면, 종내 개체 변이와 지역별 변이는 일어나지 않은 것으로 밝혀졌다. 또한 짱뚱어와 남방짱뚱어 2종간 비교 (inter-species)에서는 순천지역 짱뚱어와 해남지역 남방짱뚱어를 12S rRNA 유전자와 16S rRNA 유전자 및 mitochondria cytochrome b 유전자 염기서열을 비교 분석한 결과, 각각 96.1% (17 bp), 94.0 (29 bp), 그리고 82.9% (76 bp)로 나타나 두 종의 유전학적 차이는 크게 나타났다. 따라서 본 연구에서 나타난 짱뚱어와 남방짱뚱어 두 종간에 3.9~17.1%를 보여 서로 다른 종으로 볼 수 있을 것으로 사료되었다.

Keywords

References

  1. 김익수. 1997. 한국동식물도감. 제37권 동물편 (담수어류). 교육부, 629pp.
  2. 한상훈.김병직.김화정.이승기.안정현. 2012. 국가 생물종 목록집[척추동물]. 국립생물자원관, 484pp.
  3. Cantatore, P., M. Roberi, G. Pesole, A. Ludovico, F. Milella, M.N. Candaleta and C. Saccone. 1994. Evolutionary analysis of chtochrome b sequences in some Perciformes: evidence for a slower rate of evolution than mammals. J. Mol. Evol., 39: 589-597. https://doi.org/10.1007/BF00160404
  4. Dotsu, R. and M. Nakano. 1982. A study on the proliferation and culture of Boleophtalmus chinensis. Bull. Fish. Nagasaki Univ., 35: 1-13.
  5. Eva, G.V., Z.I. Izquierdo and J. Perez. 2006. Genetic variation at ribosomal genes supports the existence of two different European subspecies in the megrim Lepidorhombus whiffiagonis. J. Sea Res., 56: 59-64. https://doi.org/10.1016/j.seares.2006.04.001
  6. Garcia-Vazquez, C., J.I. Izquierdo and J. Perez. 2006. Genetic variation at ribosomal genes supports the existence of two different European subspecies in the megrim Lepidorhombus whiffiagonis. J. Sea Res., 56: 59-64. https://doi.org/10.1016/j.seares.2006.04.001
  7. Hillis, D.M., C. Moritz and B.K. Mable. 1996. Molecular Systematics. Sinauer Associaties, Inc, pp. 205-247.
  8. Huyse, T., J.V. Houdt and F.A.M. Volckaert. 2004. Paleoclimatic history and vicariant speciation in the "sand goby" group (Gobiidae, Teleostei). Mol. Phylogen. Evol., 32: 324-336. https://doi.org/10.1016/j.ympev.2003.11.007
  9. Iwata, A. and S.R. Jeon. 1987. First record of four gobiid fishes from Korea. Kor. J. Limnol., 20: 1-12.
  10. Jeong, S.J., K.H. Han, J.K. Kim and D.S. Sim. 2004. Age and growth of the blue spot mudskipper (Boleophtalmus pectinirostri) in the mud flat of Southwestern Korea. J. Kor. Fish. Soc., 37: 44-50.
  11. Keith, P., T. Galewski, G. Cattaneo-Berrebi, T. Hoareau and P. Berrebi. 2005. Ubiquity of Sicyopterus lagocephalus (Teleostei: Gobioidei) and phylogeography of the genus Sicyopterus in the Indo-Pacific area inferred from mitochondrial cytochrome b gene. Mol. Phylogen. Evol., 37: 721-732. https://doi.org/10.1016/j.ympev.2005.07.023
  12. Kim, I.C., H.S. Kweon, Y.J. Kim, C.B. Kim, M.C. Gye, W.O. Lee, Y.S. Lee and J.S. Lee. 2004. The complete mitochondrial genome of the javeline goby Acanthogobius hasta (Perciformes, Gobiidae) and phylogenetic considerations. Gene, 336: 147-153. https://doi.org/10.1016/j.gene.2004.04.009
  13. Kim, J.J., J.Y. Park and Y.U. Kim. 2003. Sequence variation in the mitochondrial cytochrome b genes in three mullets (Mugilidae, Pisces). Kor. J. Ichthyol., 15: 232-240.
  14. Kim, M.J., S.H. Han, H.Y. Yang, M.R. Jo, S.C. Chung and C.B. Song. 2006. Evolutionary relationship of Liobagrus mediadiposalis (Teleostei: Amblycipitidae) populations in Korea inferred from cytochrome b DNA sequences. Kor. J. Ichthyol., 18: 329-338.
  15. Kim, Y.J., W.O. Lee, J.A. Kim and J.S. Lee. 2004. Molecular phylogeny of the Gobioidei (Perciformes) from Korea inferred from mitochondrial cytochrome b gene. Kor. J. Ichthyol., 16: 51-59.
  16. Koga, H., S. Noda, T. Noguchi and I. Aoto, 1989. Studies on artificial propagation of mud skipper Boleophthalmus pectinirostris (Linnaeus) - III. Hatching and larval rearing. Bull. Saga Prefect. Ariake Fish. Exp. Stn., 11: 17-28. (in Japanese)
  17. Kumer, S., K. Tamura and M. Nei. 2004. Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinf., 5: 150-163. https://doi.org/10.1093/bib/5.2.150
  18. Lee, H.J., J.Y. Park, W.J. Kim, K.S. Min, Y. Kim, M.A. Yoo and W.H. Lee, 1999. Genetic study of the subfamily Salmoninae based upon mitochondrial DNA control region sequences. Kor. J. Ichthyol., 11: 163-171.
  19. Orti, G., M.A. Be, T.E. Reimchen and A. Meyer. 1994. Global surbey of mitochondrial DNA sequences in the threespin stickleback: evidence for recent migration. Evolution, 48: 608-622. https://doi.org/10.2307/2410473
  20. Park, G.M. and H.B. Song. 2010. Phylogenetic divergence in the sourth torrent catfish, Liobagrus mediadiposalis (Pisces; Amblycipitidae) of Korea. J. Fisher. Soc. Taiwan, 37: 173-181.
  21. Peng, L., W.O. Lee, J.H. Im, S.J. Lee, Y.K. Nam and D.S. Kim. 2004. Genetic analysis of long snout bullhead (Leiocassis longirostris) using mitochondrial cytochrome c oxidase subunit I and 12S rRNA genes. Korean J. Ichthyol., 16: 109-115.
  22. Perdices, A., C. Cunha and M.M. Coelho. 2004. Phylogenetic of Zacco platypus (Teleostei, Cyprinidae) populations on the upper and middle Chang Jiang (=Yangtze) drainage inferred from cytochrome b sequences. Mol. Phylo. Evol., 31: 192-203. https://doi.org/10.1016/j.ympev.2003.07.001
  23. Ryu, B.S., I.S. Kim and Y. Choi, 1995 Ecology and life history of Boleophthalmus pectinirostris in Korea. J. Kor. Fish. Soc., 28: 316-324.
  24. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning. Laboratory Manual. 2nd. Cold Spring Harbor Laboratory Press. USA, 655pp.
  25. Sota, T., M. Takahiko, S. Toshihiko, S. Hitoshi and Y. Kenichiro. 2005. Genetic differentiation of the gobies Gymnogobius castaneus and G. taranetizi in the region surrounding the Sea of Japan as inferred from a mitochondrial gene genealogy. Zool. Sci., 22: 87-93. https://doi.org/10.2108/zsj.22.87
  26. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL-X window interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nuc. Acids Res., 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  27. Wang, H.Y., M.P. Tsai, J. Dean and S.C. Lee. 2001. Molecular phylogeny of gobioid fishes (Perciformes: Gobioidei) based on mitochondrial 12S rRNA sequences. Mol. Phylogen. Evol., 20: 390-408. https://doi.org/10.1006/mpev.2001.0957