• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2012년도 제38회 춘계학술대회논문집
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• pp.451-453
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• 2012

7톤급 로켓엔진 가스발생기에 대한 개념설계와 제작방법에 대해서 기술하였다. 엔진 시스템 설계의 결과로서 연소실 압력, 혼합비, 전체유량이 각각 6 MPa, 0.321, 1kg/s로 결정되었다. 이 변수들을 기본으로 가스발생기의 개념설계가 수행되었고 외형 치수는 대략 ${\Phi}100{\times}250mm$ 정도이다. 가스발생기 대부분의 부품들은 브레이징이나 TIG 용접을 통해 서로 결합되며 가능한 모든 단계에서 강도/기밀시험을 수행하여 용접 부를 점검한다.

• 한국식품저장유통학회지
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• 제5권4호
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• pp.386-391
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• 1998

김치로부터 분리 동정된 Lactobacillus plantarum KLAB21 균주에 있어서 항돌연변이 활성물질 생산을 위한 최적 조건을 조사하였다. 탄소원으로서 glucose 첨가시 가장 높은 항돌연변이 활성을 나타내었으며, 질소원으로서는 yeast extract와 bactopeptone 첨가시 활성이 우수하였다. 탄소원인 glucose의 농도는 2%에서, 질소원인 yeast extract와 bactopeptone의 농도는 1%에서 가장 우수한 항돌연변이 활성을 나타내었다. 항돌연변이 활성의 최적 배양 조건은 초기 pH 배양온도, 배양속도 각각 7.0, 37$^{\circ}C$, 150rpm이었다. 상기의 최적 조건에서 36시간 배양시 가장 높은 항돌연변이 활성을 나타내었는데 S. typhimurium TA100과 S. typhimurium TA98을 이용한 조사결과 항돌연변이 활성이 각각 73.95%, 59.47%이었다.

• 생명과학회지
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• 제32권7호
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• pp.491-500
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• 2022

HK표고버섯균사체(HK shiitake mushroom mycelium, HKSMM)는 간 건강 개별 인정 건강기능식품이다. LPS로 활성화된 RAW 264.7 세포에서 HKSMM50 (HKSMM의 50% ethanol 수용액 추출물)의 항염증효과를 연구하였다. AHCC는 positive control로 사용하였다. LPS로 활성화된 RAW 264.7 세포에 HKSMM50 및 AHCC를 처리(0, 20, 100, 500 ㎍/ml)하고 24시간 배양하여 배양물의 염증 관련 인자는 ELISA kits로, 세포에 함유된 iNOS와 COX-2 protein 발현은 Western blotting으로 측정하였다. HKSMM50는 LPS 처리에 비해 농도 의존적으로 NF-κB 함량을 낮추었고, iNOS와 COX-2 protein 발현을 억제하여 NO와 PGE₂ 함량을 낮추었다. 더불어 HKSMM50는 LPS 처리에 비해 IL-1β, TNF-α, IL-4 및 IL-6의 함량을 낮추었으나 SOD와 CAT의 활성은 증가시켰다. AHCC도 HKSSM50 처리와 비슷한 효과를 나타내었다. 이 결과는 HKSMM50이 LPS로 활성화된 RAW 264.7 세포에서 NF-κB 신호전달을 억제하여 항염증효과를 나타내었으며, HKSMM은 면역기능증진에 도움을 줄 수 있는 건강기능식품원료로 사용할 수 있을 것이다.

• 한국식생활문화학회지
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• 제36권5호
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• pp.502-511
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• 2021

In this study, the content of water-soluble vitamins such as vitamin B₁ (thiamin), B₂ (riboflavin), B₃ (niacin), B₅ (pantothenic acid), B₆ (pyridoxine), B₇ (biotin), B₁₂ (cyanocobalamin), and C (ascorbic acid) in dishes containing meat and seafood consumed in Korea were determined by high-performance liquid chromatography (HPLC) with ultraviolet and fluorescence detection. All analyses were performed under strict quality control of vitamins B₁, B₂, B₃, B₅, B₆, B₇, B₁₂, and C. The highest content of vitamin B₁ was observed in Bugeo-gangjeong (1.373 mg/100 g) and the highest level of vitamin B₂ (5.162 mg/100 g) was found in pig liver. Bugeo-gangjeong showed the highest content of vitamin B₃ (21.676 mg/100 g), and kkomak-muchim contained considerable amounts (43.310 mg/100 g) of vitamin B₅. Vitamin B₆ was not detected in most seafood dishes except for yangnyeom myeongran-jeot (0.274 mg/100 g) and was present at low levels or not present at all in meat dishes. The highest content of vitamin B₇ was 6.506 ㎍/100 g in saeu-jeon and kkomak-muchim showed the highest content (21.132 ㎍/100 g) of vitamin B₁₂. The highest content of vitamin C was in yangnyeom myeongran-jeot (84.508 mg/100 g). In addition, the analysis methods of each water-soluble vitamin were verified. These results showed that seafood-based ingredients in several dishes could be a good source of water-soluble vitamins.

• Journal of Nutrition and Health
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• 제4권1호
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• pp.1-19
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• 1971

Nutritional anemia is an important nutritional problem affecting large population groups in most developing countries. Nutritional anemia is caused by the absence of any dietary essential involed in hemoglobin formation or by poor absorption of these dietary components. The most likely causes are lack of dietary iron, and folate, vitamin $B_{12}$ and high qualify protein. Anemia is considered to be a late mainfeastation of nutritional deficiencies, and even mild anemia is not the earilest sign of such a deficiency. Therefore, the object of therapy is to correct underlying deficiency rather than merely its manifestation. Iron deficiency anemia is generally much the most common form of anemia. And it is very prevalent particularly in pregnant women and young children, especially under five year of life. According to the rapid growth rate of infants, dietary iron should he provided for infants over three months of age in adequate amounts for the synthesis of hemoglobin required by the increasing blood volume and for the demands of newly formed cells. The principal causes of iron deficiency anemia are an inadequate dietary iron content, interference with absorption of iron from the intestine, excessive losses of iron from the body, disturbance of iron metabolism by infection, and social and cultural environments. The present study is planned to obtain informations concerning nutritional anemia through anthropometric and biochemical determinations for the assessment of nutriture in pre-school children. Determination was taken in 226 pre-school children in ruraI arae in 1968, 122 pre-school children in 1970, and 1526 hospitalized pre-school children in 1970. The results of this study are as follows; (1) According to Iowa Malnutrition Borderline (85 percentile) for weight, the proportions of underweighed pre-school boys and girls in rural area were 47.2% and 46.2% in1968, and were 36.1% and 51.8% in 1970. According to Iowa Malnutrition Borderline for height, the proportions of underheight boys and girls in rural area were 30.5% and 33.7%, and were 26.2% and 21.8% in 1970. Malnutrition scores of underweight for height values of boys and girls in rural area were 19.3 and 17.3 in 1968, and the scores of boys and girls were 15.6 and 15.5 in 1970. (2) The mean hemoglobin values of boys and girls in rural area were $11.2{\pm}1.8g/100ml\;and\;11.4{\pm}1.6g/100ml$ in 1968. In 1970, the mean values of boys and girls in rural area were $11.3{\pm}1.3g/100ml\;and\;11.7{\pm}2.4g/100ml$. The mean hemoglobin values of hospitalized boys and girls were $11.9{\pm}2.2g/100ml\;and\;11.7{\pm}2.4g/100ml$ in 1970. It is found that 92 of 215 children (42.7%) in rural area had concentrations of hemoglobin less than 11.0g/100ml in 1968. In 1970, 55 of 121 children (45.4%) in rural area and 559 of 1526 hospitalized children (36.6%) had concentrations of hemoglobin less than 11.0g/100ml. (3) The mean hematocrit levels of hospitalized boys and girls were $35{\pm}26.8%\;and\;35.4{\pm}6.4%$ in 1970. And 443 of 1334 hospitalized children (33.2%) had hematocrit values below 33%. (4) The average mean corpuscular hemoglobin concentration levels of hospitalized boys and girls were $32.4{\pm}2.2\;and\;32.3{\pm}2.2$ in 1970. And 1016 of 1352 hospitalized children (75.1%) had the mean corpuscular hemoglobin values below 34. (5) The mean iron values of young children in rural area and hospitalized children were $62.0{\pm}6.3{\mu}g/100ml\;and\;60.7{\pm}22.8{\mu}g/100ml$. The proportions of anemia cases below $50{\mu}g/100ml$ in rural area was 37.9%, and 34.3% in hospitalized children. (6) The mean total iron binding capacity of young children in rural area was $376{\pm}57.88{\mu}g/100ml,\;and\;342.2{\pm}6.15{\mu}g/100ml$ in hospitalized children. (7) The average transferrin saturation percentage of young children in rural area was $16.9{\pm}4.7%,\;and\;18.0{\pm}8.4%$ in hospitalized children. The proportions of anemia cases below 15% of young chi1dren in rural area and hospitalized children were 48.3% and 41.2%. Therefore, authors wish to recommend that the following further studies should be undertaken: (1) Standardization of simplied laboratory examination of nutritional anemia. (2) The prevalence of nutritional anemia and the requirements of iron, folate, and vitamin $B_{12}$ of pre-school children. (3) The content and absorption of iron in Korean food. (4) The pathogenesis of nutritional anemia and prevention of parasitic disease. (5) Maternal health and nutrition education.

• KSBB Journal
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• 제15권1호
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• pp.106-111
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• 2000

복숭아심식나방, 사고애모무늬잎말이나방 그리고 왕풍뎅이 는 전국에 퍼져있는 주요 사과원 해충들이다. 본 연구에서는 국내에서 분리된 고충병원성 성충인 Steinernema carpocapsae 와 Steunernema glaseri종을 이용하여 이들 사과원 병해충에 대한 방제효과를 조사하였다. 복숭아심식나방의 경우 S.carpocapsae를 유충 한 마리당 80마리의 농도로 처리했을 때 24시간 후에 100%의 치사율을 보였으나 S. glaseri의 경우 유충 한 마리당 50마리의 농도로 처리했을 때 48시간 후에도 $83.3\pm5.8$%의 치사율을 보였다. 사과애모무늬잎말이나방의 경우 50마리/mL 선충농도로 처리하였을 때 48시간 후에 S.carpocapsae와 S.glaseri의 처리구는 각각 100%와 43.3$\pm$5.8%의 치사율을 보였다. 그러나 왕풍뎅이 5~6령충의 경우 유츙 한 마리당 200~800마리까지 S.carpocapsae와 S.glaseri를 처리하였으나 병원성이 없는 것으로 나타났다. 선충의 토양 침투력은 유충 한 마리당 선충 접종농도가 증가할수록, 그리고 온도가 높을수록 증가되어 $25^{\circ}C$, 50마리/mL 처리구에서 S.carpocapsae가 10cm 깊이까지는 100%의 치사율을, 16~15cm깊이에서는 56.7$\pm$5.8%의 치사율을 보였다.

• 한국약용작물학회지
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• 제10권2호
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• pp.139-143
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• 2002

각 균주에 대하여 씀바귀 분말 시료들이 독성을 나타내지 않는 범위에서 강력한 발암물질로써 직접 변이원으로 사용된 $MNNG(0.4{\mu}g/plate)$의 경우 S. typhymurium TA100 균주에서 시료농도 $100{\mu}g/plate$에서 메탄올 추출물이 84.51%로 가장 높은 억제효과가 나타났으며, $4NQO(0.15{\mu}g/plate)$에 대한 S. typhymurium TA98 균주에서 시료농도 $100{\mu}g/plate$에서는 물 분획물이 86.73%로 가장 높은 억제효과를 나타내었다 또한, TA100균주의 경우에는 부탄올 분획물에서 88.93%로 가장 높은 억제효과를 나타내었다. 간접변이원인 B(a)P를 사용한 실험에서 TA98 균주에서는 메탄올 추출물이 86.98%로 가장 높은 억제효과를 보였으며, TA100 균주에서는 부탄올 분획물이 75.35%로 가장 높은 억제효과를 나타냈다. 암세포주(A549, Hep3B, MCF-7)에 대한 씀바귀 분말의 증식 억제효과를 알아보기 위하여 실험한 결과, 폐암세포주인 A549세포에서는 핵산 분획물$(500{\mu}g/ml)$이 87.91%로 가장 높은 억제효과를 나타내었으며, 간암 세포주인 Hep3B 에서는 핵산 분획물$(375{\mu}g/ml)$이 70.64%로 가장 높은 억제효과를 나타냈다. 유방암 세포주인 MCF-7에서도 핵산 분획물$(375{\mu}g/ml)$이 82.89%로 가장 높은 억제효과를 보였으며, 인간 정상 간세포 293에 대해서는 24% 이하의 생육억제율을 보였다. 이는 암세포주에 대한 높은 억제효과에 비해 정상세포에 대해서는 비교적 낮은 증식 억제효과를 나타냄을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권7호
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• pp.1019-1025
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• 2011

Toll-like receptors (TLRs) play an important role in the recognition of invading pathogens and the modulation of innate immune responses in mammals. The TLR4 and TLR7 are well known to recognize the bacterial lipopolysaccharide (LPS) and single stranded (ssRNA) ligands, respectively and play important role in host defense against Gram-negative bacteria and ssRNA viruses. In the present study, coding exon fragments of these two TLRs were identified, cloned, sequenced and analyzed in terms of insertion-deletion polymorphism, within bovine TLRs 4 and 7, thereby facilitating future TLR signaling and association studies relevant to bovine innate immunity. Comparative sequence analysis of TLR 4 exons revealed that this gene is more variable, particularly the coding frame (E3P1), while other parts showed percent identity of 95.7% to 100% at nucleotide and amino acid level, respectivley with other Bos indicus and Bos taurus breeds from different parts of the world. In comparison to TLR4, sequence analysis of TLR7 showed more conservation among different B. indicus and B. taurus breeds, except single point mutation at 324 nucleotide position (AAA to AAM) altering a single amino acid at 108 position (K to X). Percent identity of TLR7 sequences (all 3 exons) was between 99.2% to 100% at nucleotide and amino acid level, when compared with available sequence database of B. indicus and B. taurus. Simple Modular Architecture Research Tool (SMART) analysis showed variations in the exon fragments located in the Leucine Rich Repeat (LRR) region, which is responsible for binding with the microbial associated molecular patterns and further, downstream signaling to initiate anti-microbial response. Considering importance of TLR polymorphism in terms of innate immunity, further research is warranted.

• 한국산학기술학회논문지
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• 제16권7호
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• pp.5029-5037
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• 2015

본 연구의 목적인 우리나라 일부 학교의 석면함유 건축자재을 조사하고 EPA AHERA rule과 ASTM rule을 적용하여 위해성평가를 실시하였다. 총 100개 학교를 2010년 1월부터 12월까지 조사하였다. 건축년도에 따른 ACM 검출율은 1980년대 이전 건축물은 100%, 1990년대 이전은 94.1%, 2000년대 이전은 100%, 2000대 이후는 62.5%로 나타났다. 학교별 ACM 검출율은 유치원과 고등학교는 100%, 초등학교는 97.1%, 중학교는 92.9%, 특수학교는 80%로 나타났다. 천장 텍스는 2~8 %의 백석면과 갈석면, 밤라이트 보드는 6~11 %의 백석면이 검출되었다. 또한 개스킷과 슬레이트 지붕은 각각 16~17 %, 10~13 %의 백석면이 검출되었다. EPA AHERA rule을 이용하여 ACM의 위해성 평가를 실시한 결과, 모든 건축자재는 "Pool" 등급이 나왔으며, ASTM rule 평가에서는 모든 ACM이 "Q&M program"이 필요한 것으로 나타났다.

• 한국운동역학회지
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• 제17권4호
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• pp.149-156
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• 2007

The purpose of this study was to compare the world class women's hurdlers with kinematic variables Lee Yeon-Kyoung's in the 100m hurdle. Among korea elite female hurdler, Lee Yeon-Kyoung was participated as a subject. Eight JVC video cameras(GR-HD1KR) were used to film the performance of Lee Yeon-Kyoung at a frame rate of 60fields/s. The real-life three-dimensional coordinate data of digitized body landmarks were smoothed using a fourth order Butterworth low pass recursive digital filter with an estimated optimum cutoff frequency of 7.4Hz. After analyzing and comparing Lee Yeon Kyung's kinematic variables with the world top class hurdlers in the woman's 100m hurdle run, the following conclusions were obtained. 1. Lee should be able to increase the speed with over 5.4m/s from start to first hurdle and then maintain the speed range from 8.33m/s to 8.67m/s until 10th hurdle. Lee should have to maintain the speed with 8.51m/s from 10th hurdle to finish line. 2. Lee has to reach her maximum running speed at 5th hurdle and then has to shorten running time with 0.5sec between hurdles. 3. Lee should be able to run around 2.5sec from start to frist hurdle and then maintain under 1.00sec following phases. Lee should be able to maintain under 1.10sec from 10th hurdle to finish line. 4. Lee needs to control a consistent takeoff and landing distance pattern, Lee needs to lower the height of the center of gravity of the body with 0.33m when she clears the hurdles.