Anti-inflammatory Efficacy of HK Shiitake Mushroom Mycelium in LPS-treated RAW 264.7 Cells Through Down-regulation of NF-κB Activation
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Song, Chae Yeong
(HK Biotech. Co., Ltd.)
Oh, Tae Woo (Korea Institute of Oriental Medicine) Kim, Hoon Hwan (College of Veterinary Medicine, Gyeongsang National University) Lee, Yu Bin (HK Biotech. Co., Ltd.) Kim, Jeong Ok (HK Biotech. Co., Ltd.) Kim, Gon Sup (College of Veterinary Medicine, Gyeongsang National University) Ha, Yeong Lae (HK Biotech. Co., Ltd.) |
1 | Crespo, H., Guillen, H., de Pablo-Maiso, L., Gomez-Arrebola, C., Rodriguez, G., Glaria, I., de Andres, D. and Reina, R. 2017. Lentinula edodes β-glucan enriched diet induces proand anti-inflammatory macrophages in rabbit. Food Nutr. Res. 61, 141279. |
2 | Du, B., Lin, C., Bian, Z. and Xu, B. 2015. An insight into anti-inflammatory effects of fungal beta-glucan. Trands Food Sci. Technol. 41, 49-59. DOI |
3 | Garcia-Lafuente, A., Moro, C., Cillares, A., Guillamon, E., Rostagno, M. A., Arrigo, M. D. and Martinez, A. 2010. Mushrooms as a source of anti-inflammatory agents & anti-allergy agents. Med. Chem. 9. 125-141. |
4 | Takanari, J., Hirayama, Y., Homma, K., Miura, T., Nishioka, H. and Maeda, T. 2015. Effects of active hexose correlated compound on the seasonal variations of immune competence in healthy subjects. J. Evid. Based Complementary Altern. Med. 20, 28-34. DOI |
5 | Van, Q., Nayak, B., Reimer, M., Jones, P., Fulcher, R. and Rempel, C. 2009. Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay. J. Ethnopharmacol. 125, 487-493. DOI |
6 | Robaszkiewicz, A., Bartosz, G., Lawrynowicz, M. and Soszynski, M. 2010. The role of polyphenols, beta-cartene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms. J. Nutr. Metab. 2010, 173274. doi: 10.1155/2010/173274. DOI |
7 | Ha, Y. L., Kim, Y. S., Ahn, C. R., Kewon, J. M., Park, C. W., Ha, Y. K. and Kim, J. O. 2010. Mycelial culture of Lentinus edodes alleviates rat liver toxicity induced by carbon tetrachloride and ethanol. J. Life Sci. 20, 133-141. DOI |
8 | Kuo, M. C., Weng, C. Y., Ha, C. L. and Wu, M. J. 2006. Ganoderma lucidum mycelia enhance innate immunity by activating NF-κB. J. Ethnopharmacol. 103, 217-222. DOI |
9 | Lee, J. W. 2014. Antioxidant and immunological activities of polysaccharide extracted from cultured mycelia of Schizophyllum commune. J. Kor. Soc. Food Sci. Nutr. 49, 1334-1341. DOI |
10 | Sajon, S. R., Sana, S., Rana, S., Mushiur, S. M. and Nishi, X. M. 2018. Mushrooms: Natural factory of anti-oxidant, anti-inflammatory, analgesic and nutrition. J. Pharmacogn. Phytochem. 7, 464-475. |
11 | Raha, S., Lee, H. J., Hong, G. E., Venkatarame, V., Saralamma, G., Ha, Y. L., Kim, J. O., Kim, Y. S., Heo, J. D., Lee, S. J, Kim, E. H. and Kim, G. S. 2016. Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway. Life Sci. 161, 37-44. DOI |
12 | Xu, X., Yasuda, M., Mizuno, M. and Ashida, H. 2012. β-Glucan from Saccharomyces cerevisiae reduces lipopolysaccharide-induced inflammatory responses in RAW264.7 macrophages. Biochim. Biophys. Acta. 820, 1656-1663. |
13 | Yoon, H. M., Jang, K. J., Han, M. S., Jeong, J. W., Kim, G. Y., Lee, J. H. and Choi, Y. H. 2013. Ganoderma lucidum ethanol extract inhibits the inflammatory response by suppressing the NF-κB and toll-like receptor pathways in lipopolysaccharide-stimulated BV2 microglial cells. Exp. Therap. Med. 5, 957-963. DOI |
14 | Seo, K. H., Park, J. Y., Noh, H. J., Lee, J. Y., Lee, E. Y., Han, J. G., Kim, J. H. and Cheong, M. S. 2018. Anti-inflammatory effects of various mushrooms in LPS-stimulated RAW264.7 cells. Kor. J. Plant Res. 31, 478-488. DOI |
15 | Taofiq, O., Calhelha, R. C., Heleno, S., Barros, L., Martins, A., Santos-Buelga, C., Queiroz, M. J. R. and Ferreira, I. C. 2015. The contribution of phenolic acids to the anti-inflammatory activity of mushrooms: Screening in phenolic extracts, individual parent molecules and synthesized glucuronated and methylated derivatives. Food Res. Int. 76, 821-827. DOI |
16 | Trianthi, P. and Aggarwal, A. 2006. NF-kB transcription.. factor: a key player in the generation of immune response. Curr. Sci. 90, 519-531. |
17 | Xu, X., Yasuda, M., Nakamura-Tsuruta, S., Mizuno, M. and Ashida, H. 2012. beta-Glucan from Lentinus edodes inhibits nitric oxide and tumor necrosis factor-a production and phosphorylation of mitogen-activated protein kinases in lipopolysaccharide-stimulated murine RAW 264.7 macrophages. J. Biol. Chem. 287, 871-878. DOI |
18 | Du, C., Bhatia, M., Tang, S. C., Zhang, M. and Steiner, T. 2015. Mediators of inflammation: Inflammation in cancer, chronic diseases, and wound healing. Mediators Inflamm. 2015, 570653. |
19 | Bisen, P. S., Baghel, R. K., Sanodiya, B. S., Thakur, G. S. and Prasad, G. B. K. S. 2010. Lentinus edodes: A macrofungus with pharmacological activities. Curr. Med. Chem. 17, 2419-2430. DOI |
20 | Chien, R. C., Yang, Y. C., Lai, E. I. and Mau, J. L. 2016. Anti-inflammatory effects of extracts from the medicinal mushrooms Hypsizygus marmoreus and Pleurotus eryngii (Agaricomycetes). Int. J. Med. Mushrooms 18, 477-487. DOI |
21 | Finimundy, T. C., Jose, A., Dillon, P., Antonio. J., Henriques, P. and Ely, M. R. 2014. A review on general nutritional compounds and pharmacological properties of the Lentinula edodes mushroom. Food Nutr. Sci. 5, 1095-1105. DOI |
22 | Han, H. S. 2021. Effect of water extract of Sparasis crispa on the expression of TNF-α, iNOS and IL-1β genes in RAW 264.7 cells. J. Kor. Soc. Integr. Med. 9, 163-171. DOI |
23 | Fiorucci, S., Santucci, L., Wallace, J. L., Sardina, M., Romano, M., del Soldato, P. and Morelli, A. 2003. Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa. PNAS 100, 10937-10941. DOI |
24 | Guzik, T. J., Korbut, R. and Adamek-Guzik, T. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54, 469-487. |
25 | Gunawardena, D., Bennett, L., Shanmugam, K., King, K., Williams, R., Zabaras, D., Head, R., Ooi, L., Gyengesi, E. and Munch, G. 2014. Anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages. Food Chem. 148, 92-96. DOI |
26 | Carbonero, E. R., Gracher, A. H. P., Komura, D. L., Marcon, R., Freitas, C. S., Baggio, C. H. and Santos, A. R. S. 2008. Lentinus edodes heterogalactan: Antinociceptive and anti-inflammatory effects. Food Chem. 111, 531-537. DOI |
27 | Lin, J. T., Liu, C. W., Chen, Y. C., Hu, C. C., Juang, L. D., Shiesh, C. C. and Yang, D. J. 2014. Chemical composition, antioxidant and anti-inflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time. LWT-Food Sci. Technol. 55, 374-382. DOI |
28 | Liu, T., Zhang, L., Ju, D. and Sun, S. C. 2017. NF-kB signaling in inflammation. Signal Transduct. Target. Ther. 2, e17023. |
29 | Liu, Y. C., Zou, X. B., Chai, Y. F. and Yao, Y. M. 2014. Macrophage polarization in inflammatory diseases. Int. J. Biol. Sci. 10, 520-529. DOI |
30 | Jedinak, A., Dudhgaonkar, S., Wu, O. L., Simon, J. and Sliva1, D. 2011. Anti-inflammatory activity of edible oyster mushrooms is mediated through the inhibition of NF-kB and AP-1 signaling. Nutr. J. 10, 52. DOI: 10.1186/1475-2891-10-52. DOI |
31 | Jang, W. J, Kim, Y. S, Ha, Y. L, Park, C. W, Ha, Y. K. and Kim, O. K. 2010. Optimal level for the protection of carbon tetrachloride-induced Sprague-Dawley rat liver damage by mycelial cultures of Lentinus edodes. J. Life Sci. 20, 782-788. DOI |
32 | Libby, P., Ridker, P. M. and Maseri, A. 2002. Inflammation and atherosclerosis. Circulation 105, 1135-1143. DOI |
33 | Kim, J. I. 2009. Efficacy of the blood lipid improvement, antioxidant, and liver function improvement of shiitake mushroom mycelium powder in the high alcohol intake group. Inje University. Final report. |
34 | Kim, D. H., Park, S. R., Debnath, T., Hasnat, A., Pervin, M. and Lim, B. O. 2013. Evaluation of the antioxidant activity and anti-inflammatory effect Hericium erinaceus water extracts. Kor. J. Medicinal Crop Sci. 21, 112-117. DOI |
35 | Park, S. O. and Kim, J. M. 2012. Functional food for immune regulation beta-glucan. Food Sci. Ind. 45, 39-47. DOI |
36 | Nagappan, A., Varghese, J., Pranesh, G. T., Jeyaseelan, V. and Jacob, M. 2014. Indomethacin inhibits activation of endothelial nitric oxide synthase in the rat kidney: possible role of this effect in the pathogenesis of indomethacininduced renal damage. Chem. Biol. Interact. 25, 77-87. |
37 | Ma, L., Chen, H., Dong, P. and Lu, X. 2013. Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus. Food Chem. 139, 503-508. DOI |
38 | Mann, J. R., Backlund, M. G. and DuBois, R. N. 2005. Mechanisms of disease: Inflammatory mediators and cancer prevention. Nat. Clin. Pract. Oncol. 2, 202-210. DOI |
39 | Martinez, F. O., Sica, A., Mantovani, A. and Locati, M. 2008. Macrophage activation and polarization. Front. Biosci. 13, 453-461. DOI |
40 | Ministry of Food and Drug Safety. 2010. HK shiitake mushroom mycelium. Certificate of health functional food ingredient for liver health. 2010-35. |
41 | Park, Y. M., Won, J. H., Kim, Choi, W., Park, H. J. and Lee, K. T. 2005. In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus. J. Ethnopharmacol. 101, 120-128. DOI |
42 | Ministry of Food and Drug Safety. 2022. Standards and specifications of health functional foods. 3-25(237p), 3048 (361p) and 3-53(388p). |
43 | Parola, S., Chiodaroli, L., Orlandi, V., Vannini, C. and Panno, L. 2017. Lentinula edodes and Pleurotus ostreatus: functional food with antioxidant-antimicrobial activity and an important source of Vitamin D and medicinal compounds. Funct. Foods Health Dis. 7, 773-794. DOI |
44 | Rodrigues, M. L. 2018. The multifunctional fungal ergosterol. ASM J. 9, 01755-18. |
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