• Journal of Bio-Environment Control
• /
• v.22 no.3
• /
• pp.228-233
• /
• 2013

This study was conducted to examine the effect of light intensity and photoperiod of white LEDs as the artificial light source on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings, transplanted at a density of $20cm{\times}20cm$ in a completely randomized design, were grown under white LEDs (FC Poibe Co. Ltd., Korea), at one of the 3 light intensities (100, 200, or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), and each with one of 3 photoperiods [12/12, 18/6, or 24/0 (Light/Dark)]. Plants were cultured for 22 days under the condition of $21{\pm}2^{\circ}C$, $60{\pm}10%$ RH, and $400{\pm}50{\mu}mol{\cdot}mol^{-1}\;CO_2$. The greatest leaf length and width, fresh and dry weights, and total anthocyanin content were obtained in the 24/0 photoperiod, regardless of the light intensity. Length of the longest root, fresh and dry weights, and number of leaves were greater in light intensity of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ than 100 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Chlorophyll value was the greatest in the photoperiod 12/12 than 18/6 or 24/0. The results obtained suggest that plant grew the best kept by light intensity at 200 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and photoperiod of 12/12 or 18/6.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.41 no.9
• /
• pp.25-30
• /
• 2004

In this Paper, the 0.1 w InGaAs/InAlAs/GaAs Metamorphic HEMT, which is applicable to MIMIC, and a 100 GHz MIMIC amplifier were designed and fabricated. The DC characteristics of MHEMT are 640 mA/mm of drain current density, 653 mS/mm of maximum transconductance. The current gain cut-off frequency(fT) is 173 GHz and the maximum oscillation frequency(fmax) is 271 GHz. A 100 GHz amplifier was designed using 0.1${\mu}{\textrm}{m}$ MHEMT and CPW technology. The measured results from the 100 GHz MIMIC amplifiers show good S21 gain of 10.1 dB and 12.74 dB at 100 GHz and 97.8 GHz, respectively.

• IMMUNE NETWORK
• /
• v.2 no.3
• /
• pp.175-181
• /
• 2002

Background: S100A6 is a calcium-binding protein overexpressed in several tumor cell lines including melanoma with high metastatic activity and involved in various cellular processes such as cell division and differentiation. To detect S100A6 protein in patient' samples (ex, blood or tissue), it is essential to produce a monoclonal antibody specific to the protein. Methods: First, cDNA coding for ORF region of human S100A6 gene was amplified and cloned into the expression vector for GST fusion protein. We have produced recombinant S100A6 protein and subsequently, monoclonal antibodies to the protein. The specificity of anti-S100A6 monoclonal antibody was confirmed using recombinant S100A recombinant proteins of other S100A family (GST-S100A1, GST-S100A2 and GST-S100A4) and the cell lysates of several human cell lines. Also, to identify the specific recognition site of the monoclonal antibody, we have performed the immunoblot analysis with serially deleted S100A6 recombinant proteins. Results: GST-S100A6 recombinant protein was induced and purified. And then S100A6 protein excluding GST protein was obtained and monoclonal antibody to the protein was produced. Monoclonal antibody (K02C12-1; patent number, 330311) has no cross-reaction to several other S100 family proteins. It appears that anti-S100A6 monoclonal antibody reacts with the region containing the amino acid sequence from 46 to 61 of S100A6 protein. Conclusion: These data suggest that anti-S100A6 monoclonal antibody produced can be very useful in development of diagnostic system for S100A6 protein.

• Korean Journal of Microbiology
• /
• v.20 no.1
• /
• pp.11-20
• /
• 1982

A study was undertaken to examine the effect of curing agents on the stability, curing and segregation of R plasmid pSL100. And also the stability, transfer frequency, and recombination of its segregants obtained from curing agent treatment were studied. Ethidium bromide, acridine orange, and mitomycin-C were used as curing agent. The results obtained were as follows ; 1. The curing agent ethidium bromide, acridine orange, and mitomycin-C were not effective for curing the multiple antibiotic resistant determinant of pSL100 in Salmonella typhimurium and Escherichia coli. However, they induced plasmid segregation with high frequency in S.typhimuruim LT-2strains. TcApSmCm, TcSmCmKm, TcApCm, TcAp, TcKm, Tc segregants were obtained. 2. The resistant markers of the segregents were transferred to S.typhimurium LT-2 strains with high frequencies whereas they were transferred to E.coli K-12 only with low frequencies. 3. The transconjugants obtained from conjugation between two different S.typhimurium segregants were similar to the phenotype of the original R factor pSL100 and the resistant markers were transferred to the S.typhimurium LT-2 or E.coli strain with equal frequencies, indicating that they are recombinants. 4. The transconjugants obtained from conjugation between pSL100 segrgants and pKM101, or pBR322 possessed the resistant markers of the two parental plasmids and they were transferred to both S.typhimurium and E.coli K-12 strains with the same frequencies and maintained stably, suggesting that they are also recombinants. 5. The recombinant pSL100 could be also obtained in rec A-strains of E.coli, suggesting that the gene function of rec A is required for the recombination of pSL100 segregants in E.coli.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.3 no.4
• /
• pp.211-216
• /
• 2003

This work describes a 3 V 12b 100 MS/s CMOS digital-to-analog converter (DAC) for high-speed communication system applications. The proposed DAC is composed of a unit current-cell matrix for 8 MSBs and a binary-weighted array for 4 LSBs, trading-off linearity, power consumption, chip area, and glitch energy with this process. The low-glitch switch driving circuits are employed to improve linearity and dynamic performance. Current sources of the DAC are laid out separately from the current-cell switch matrix core block to reduce transient noise coupling. The prototype DAC is implemented in a 0.35 um n-well single-poly quad-metal CMOS technology and the measured DNL and INL are within ${\pm}0.75$ LSB and ${\pm}1.73$ LSB at 12b, respectively. The spurious-free dynamic range (SFDR) is 64 dB at 100 MS/s with a 10 MHz input sinewave. The DAC dissipates 91 mW at 3 V and occupies the active die area of $2.2{\;}mm{\;}{\times}{\;}2.0{\;}mm$

• Toxicological Research
• /
• v.19 no.3
• /
• pp.181-187
• /
• 2003

Effects of repeated treatment with butylated hydroxyanisole (BHA) on the induction of glutathione S-transferases (GSTs) and teratogenicity of cyclophosphamide were investigated in rats. Pregnant rats were orally treated with BHA (50 mg/kg) for 7 days, from days 6 to 12 of gestation, and intraperitoneally challenged with cyclophosphamide (15 mg/kg) 2 hr after the final treatment. On day 20 of gestation, the maternal and fetal abnormalities were examined. Separately, a part of rats was sacrificed for the assay of hepatic and placental GSTs activities on day 12 of gestation following 7-day treatment with BHA. Cyclophosphamide, administered on day 12 of gestation, induced 43.2% of fetal death and resorption, and 100% of malformations in live fetuses, in contrast to low fetal resorption (8.7%) and malformations (8%) in control group. The malformations include cranial defect and exencephaly (100%), micrognathia and tongue extrusion (100%), limb defects (40%), renal pelvic dilatation (39%), and cleft palate (15%). Interestingly, BHA induced GSTs activities by 62% and 46% over the control in liver and placenta, respectively, and remarkably reduced the fetal resorption (13.9%) and malformations, resulting in 62% of cranial defect and exencephaly, 68% of micrognathia and tongue extrusion, 29% of limb defects, and 14% of renal pelvic dilatation. Taken together, it is suggested that a long-term pretreatment with BHA could substantially prevent fetuses from abortion and malformations following intrauterine exposure to teratogens including cyclophosphamide by inducing phase II antioxidant enzymes such as GSTs.

• International Journal of Internet, Broadcasting and Communication
• /
• v.15 no.4
• /
• pp.107-114
• /
• 2023

In this paper, the results of experiments conducted in indoor wired and outdoor wireless environments to reduce the interference impact of 5G mobile communication on fixed satellite services using RF Interference Reduction Module (IRM) are presented. Test results for interference signals with a 100MHz bandwidth in a wired environment demonstrate that RF IRM can reduce interference by more than 20dB in a 100MHz bandwidth. The performance of RF IRM was also tested in the S-band wireless environments. In the S-band wireless environments with interference signals having a 20MHz bandwidth, RF IRM exhibits interference reduction performance of 12-15dB, for signals with a 50MHz bandwidth, it shows 10-12dB interference reduction, and for signals with a 100MHz bandwidth, it demonstrates 5-10dB interference reduction. This paper shows that RF IRM can be used not only within transceivers but also in wireless environments to mitigate interference.

• International Journal of High-Rise Buildings
• /
• v.12 no.1
• /
• pp.1-9
• /
• 2023

The last 30 years have seen a rapid growth in the number of supertall buildings across the world, with newly completed buildings continuing to rank among the tallest 100 every year. Chinese projects notably play a pivotal role in these constant updates. It is caused by the interweaving of population and urbanization, economic considerations, and further, a series of accompanying urban problems. This paper focuses on the world's 100 tallest buildings in the last 30 years, and compares this collection of projects across five years via the dimensions of height, distribution, function and structural material. The intention is to discuss and interpret the influence factors and developing trends, some of which have been apparent over a dozen years, while others are just beginning to take shape, thus to provide an opportunity to preview the types of supertall buildings in the future.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.40 no.2
• /
• pp.104-110
• /
• 2003

A new direct digital frequency synthesizer (DDFS) is proposed. The DDFS uses a new ROM compression method that divides each ROM in the conventional DDFS into two ROMs (a quantization ROM and an error ROM). The total size of the ROMs in the proposed DDFS is significantly reduced compared to the original ROM. The ROM compression ratio of 78 is achieved for a DDFS with 12bit output data. A DDFS with 12bit output data for sine function was implemented in a 0.35${\mu}{\textrm}{m}$ CMOS technology. The power dissipation is 9.56㎽ at 100MHz with 3.3V and the maximum operating clock frequency is 330MHz.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.1
• /
• pp.44-51
• /
• 2012

Calcium ions play an important role in the establishment and maintenance of pregnancy, but molecular and cellular regulatory mechanisms of calcium ion action in the uterine endometrium are not fully understood in pigs. Previously, we have shown that calcium regulatory molecules, transient receptor potential vanilloid type 5 (TRPV6) and calbindin-D9k (S100G), are expressed in the uterine endometrium during the estrous cycle and pregnancy in a pregnancy status- and stage-specific manner, and that estrogen of conceptus origin increases endometrial TRPV6 expression. However, regulation of S100G expression in the uterine endometrium and conceptus expression of S100G has been not determined during early pregnancy. Thus, we investigated regulation of S100G expression by estrogen and interleukin-$1{\beta}$ (IL1B) in the uterine endometrium and conceptus expression of S100G during early pregnancy in pigs. We obtained uterine endometrial tissues from day (D) 12 of the estrous cycle and treated with combinations of steroid hormones, estradiol-$17{\beta}$ ($E_2$) and progesterone ($P_4$), and increasing doses of IL1B. Real-time RT-PCR analysis showed that $E_2$ and IL1B increased S100G mRNA levels in the uterine endometrium, and conceptuses expressed S100G mRNA during early pregnancy, as determined by RT-PCR analysis. To determine if endometrial expression of S100G mRNA during the implantation period was affected by the somatic cell nuclear transfer (SCNT) procedure, we compared S100G mRNA levels in the uterine endometrium from gilts with SCNT-derived conceptuses with those from gilts with conceptuses derived from natural mating on D12 of pregnancy. Real-time RT-PCR analysis showed that levels of S100G mRNA in the uterine endometrium from gilts carrying SCNT-derived conceptuses was significantly lower than those from gilts carrying conceptuses derived from natural mating. These results showed that S100G expression in the uterine endometrium was regulated by estrogen and IL1B of conceptus origin, and affected by the SCNT procedure during early pregnancy. These suggest that conceptus signals regulate S100G, an intracellular calcium transport protein, for the establishment of pregnancy in pigs.