• 생물환경조절학회지
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• 제22권3호
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• pp.228-233
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• 2013

본 실험은 식물공장시스템에서 인공광원인 백색 LED의 광도와 광주기에 따른 '선홍적축면'의 생육조건을 구명하고자 수행하였다. 그리고 각각의 챔버에 백색 LED를 설치 후 완전임의배치법으로 $20cm{\times}20cm$ 간격으로 재식하였다. 광도는 100, 200, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$로 처리하였고, 광주기는 12/12, 18/6, 24/0(명기/암기)으로 처리하였다. 온도는 $21{\pm}2^{\circ}C$, 상대습도는 $60{\pm}10%$로 조절하여 22일간 재배하였다. 엽폭과 엽장, 생체중과 건물중, 총 안토시아닌 함량은 24/0(명기/암기) 처리에서 좋았다. 최대근장, 생체중과 건물중, 엽수는 100, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$보다 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ 처리에서 좋았다. 엽록소는 광주기 18/6과 24/0 처리 보다 12/12(명기/암기) 처리에서 높았다. 본 실험의 결과로 광도는 200, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기는 12/12 또는 18/6(명기/암기) 처리를 하는 것이 좋을 것으로 판단된다.

• 대한전자공학회논문지SD
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• 제41권9호
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• pp.25-30
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• 2004

본 논문에서는 0.1㎛ InGaAs/InAlAs/GaAs Metamorphic HEMT (High Electron Mobility Transistor)를 이용하여 100 GHz 대역의 MIMIC(Millimeter-wave Monolithic Integrated Circuit) 증폭기를 설계 및 제작하였다. MIMIC 증폭기의 제작을 위해 Metamorphic HEMT(MHEMT)를 설계 및 제작하였으며, 제작된 MHEMT의 드레인 전류 밀도는 640 mA/mm 최대 전달컨덕턴스(Gm)는 653 mS/mm를 얻었으며, RF 특성으로 fT는 173 GHz, fmax는 271 GHz의 양호한 성능을 나타내었다. 100 GHz MIMIC 증폭기의 개발을 위해 MHEMT의 소신호 모델과 CPW 라이브러리를 구축하였으며, 이를 이용하여 MIMIC 증폭기를 설계하였다. 설계된 증폭기는 본 연구에서 개발된 MHEMT MIMIC 공정을 이용해 제작되었으며, 100 GHz MIMIC 증폭기의 측정결과, 100 GHz에서 10.1 dB 및 97.8 Gllz에서 12.74 dB의 양호한 S21 이득 특성을 나타내었다.

• IMMUNE NETWORK
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• 제2권3호
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• pp.175-181
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• 2002

Background: S100A6 is a calcium-binding protein overexpressed in several tumor cell lines including melanoma with high metastatic activity and involved in various cellular processes such as cell division and differentiation. To detect S100A6 protein in patient' samples (ex, blood or tissue), it is essential to produce a monoclonal antibody specific to the protein. Methods: First, cDNA coding for ORF region of human S100A6 gene was amplified and cloned into the expression vector for GST fusion protein. We have produced recombinant S100A6 protein and subsequently, monoclonal antibodies to the protein. The specificity of anti-S100A6 monoclonal antibody was confirmed using recombinant S100A recombinant proteins of other S100A family (GST-S100A1, GST-S100A2 and GST-S100A4) and the cell lysates of several human cell lines. Also, to identify the specific recognition site of the monoclonal antibody, we have performed the immunoblot analysis with serially deleted S100A6 recombinant proteins. Results: GST-S100A6 recombinant protein was induced and purified. And then S100A6 protein excluding GST protein was obtained and monoclonal antibody to the protein was produced. Monoclonal antibody (K02C12-1; patent number, 330311) has no cross-reaction to several other S100 family proteins. It appears that anti-S100A6 monoclonal antibody reacts with the region containing the amino acid sequence from 46 to 61 of S100A6 protein. Conclusion: These data suggest that anti-S100A6 monoclonal antibody produced can be very useful in development of diagnostic system for S100A6 protein.

• 미생물학회지
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• 제20권1호
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• pp.11-20
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• 1982

A study was undertaken to examine the effect of curing agents on the stability, curing and segregation of R plasmid pSL100. And also the stability, transfer frequency, and recombination of its segregants obtained from curing agent treatment were studied. Ethidium bromide, acridine orange, and mitomycin-C were used as curing agent. The results obtained were as follows ; 1. The curing agent ethidium bromide, acridine orange, and mitomycin-C were not effective for curing the multiple antibiotic resistant determinant of pSL100 in Salmonella typhimurium and Escherichia coli. However, they induced plasmid segregation with high frequency in S.typhimuruim LT-2strains. TcApSmCm, TcSmCmKm, TcApCm, TcAp, TcKm, Tc segregants were obtained. 2. The resistant markers of the segregents were transferred to S.typhimurium LT-2 strains with high frequencies whereas they were transferred to E.coli K-12 only with low frequencies. 3. The transconjugants obtained from conjugation between two different S.typhimurium segregants were similar to the phenotype of the original R factor pSL100 and the resistant markers were transferred to the S.typhimurium LT-2 or E.coli strain with equal frequencies, indicating that they are recombinants. 4. The transconjugants obtained from conjugation between pSL100 segrgants and pKM101, or pBR322 possessed the resistant markers of the two parental plasmids and they were transferred to both S.typhimurium and E.coli K-12 strains with the same frequencies and maintained stably, suggesting that they are also recombinants. 5. The recombinant pSL100 could be also obtained in rec A-strains of E.coli, suggesting that the gene function of rec A is required for the recombination of pSL100 segregants in E.coli.

• JSTS:Journal of Semiconductor Technology and Science
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• 제3권4호
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• pp.211-216
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• 2003

This work describes a 3 V 12b 100 MS/s CMOS digital-to-analog converter (DAC) for high-speed communication system applications. The proposed DAC is composed of a unit current-cell matrix for 8 MSBs and a binary-weighted array for 4 LSBs, trading-off linearity, power consumption, chip area, and glitch energy with this process. The low-glitch switch driving circuits are employed to improve linearity and dynamic performance. Current sources of the DAC are laid out separately from the current-cell switch matrix core block to reduce transient noise coupling. The prototype DAC is implemented in a 0.35 um n-well single-poly quad-metal CMOS technology and the measured DNL and INL are within ${\pm}0.75$ LSB and ${\pm}1.73$ LSB at 12b, respectively. The spurious-free dynamic range (SFDR) is 64 dB at 100 MS/s with a 10 MHz input sinewave. The DAC dissipates 91 mW at 3 V and occupies the active die area of $2.2{\;}mm{\;}{\times}{\;}2.0{\;}mm$

• Toxicological Research
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• 제19권3호
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• pp.181-187
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• 2003

Effects of repeated treatment with butylated hydroxyanisole (BHA) on the induction of glutathione S-transferases (GSTs) and teratogenicity of cyclophosphamide were investigated in rats. Pregnant rats were orally treated with BHA (50 mg/kg) for 7 days, from days 6 to 12 of gestation, and intraperitoneally challenged with cyclophosphamide (15 mg/kg) 2 hr after the final treatment. On day 20 of gestation, the maternal and fetal abnormalities were examined. Separately, a part of rats was sacrificed for the assay of hepatic and placental GSTs activities on day 12 of gestation following 7-day treatment with BHA. Cyclophosphamide, administered on day 12 of gestation, induced 43.2% of fetal death and resorption, and 100% of malformations in live fetuses, in contrast to low fetal resorption (8.7%) and malformations (8%) in control group. The malformations include cranial defect and exencephaly (100%), micrognathia and tongue extrusion (100%), limb defects (40%), renal pelvic dilatation (39%), and cleft palate (15%). Interestingly, BHA induced GSTs activities by 62% and 46% over the control in liver and placenta, respectively, and remarkably reduced the fetal resorption (13.9%) and malformations, resulting in 62% of cranial defect and exencephaly, 68% of micrognathia and tongue extrusion, 29% of limb defects, and 14% of renal pelvic dilatation. Taken together, it is suggested that a long-term pretreatment with BHA could substantially prevent fetuses from abortion and malformations following intrauterine exposure to teratogens including cyclophosphamide by inducing phase II antioxidant enzymes such as GSTs.

• International Journal of Internet, Broadcasting and Communication
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• 제15권4호
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• pp.107-114
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• 2023

In this paper, the results of experiments conducted in indoor wired and outdoor wireless environments to reduce the interference impact of 5G mobile communication on fixed satellite services using RF Interference Reduction Module (IRM) are presented. Test results for interference signals with a 100MHz bandwidth in a wired environment demonstrate that RF IRM can reduce interference by more than 20dB in a 100MHz bandwidth. The performance of RF IRM was also tested in the S-band wireless environments. In the S-band wireless environments with interference signals having a 20MHz bandwidth, RF IRM exhibits interference reduction performance of 12-15dB, for signals with a 50MHz bandwidth, it shows 10-12dB interference reduction, and for signals with a 100MHz bandwidth, it demonstrates 5-10dB interference reduction. This paper shows that RF IRM can be used not only within transceivers but also in wireless environments to mitigate interference.

• 국제초고층학회논문집
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• 제12권1호
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• pp.1-9
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• 2023

The last 30 years have seen a rapid growth in the number of supertall buildings across the world, with newly completed buildings continuing to rank among the tallest 100 every year. Chinese projects notably play a pivotal role in these constant updates. It is caused by the interweaving of population and urbanization, economic considerations, and further, a series of accompanying urban problems. This paper focuses on the world's 100 tallest buildings in the last 30 years, and compares this collection of projects across five years via the dimensions of height, distribution, function and structural material. The intention is to discuss and interpret the influence factors and developing trends, some of which have been apparent over a dozen years, while others are just beginning to take shape, thus to provide an opportunity to preview the types of supertall buildings in the future.

• 대한전자공학회논문지SD
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• 제40권2호
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• pp.104-110
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• 2003

새로운 직접 디지털 주파수 합성기(DDFS)가 제안되었다. 제안된 DDFS는 기존의 DDFS 에서의 각 롬(ROM)들을 양자화롬과 오차롬으로 나누어 저장하는 새로운 롬 압축 방식을 사용한다. 제안된 DDFS에서의 전체 롬 크기는 기존의 롬에 비하여 상당히 줄어들었다. 12비트 출력 데이터를 가지는 DDFS의 경우, 롬 압축률은 78분의 1에 이른다. 성능 검증을 위하여 사인 함수의 12비트 출력 데이터를 가지는 DDFS가 0.35㎛ CMOS 공정으로 구현되었다. 3.3V전원과 100㎒ 클럭에서의 소모 전력은 9.36㎽이고 최고 동작 클럭 주파수는 330㎒이다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권1호
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• pp.44-51
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• 2012

Calcium ions play an important role in the establishment and maintenance of pregnancy, but molecular and cellular regulatory mechanisms of calcium ion action in the uterine endometrium are not fully understood in pigs. Previously, we have shown that calcium regulatory molecules, transient receptor potential vanilloid type 5 (TRPV6) and calbindin-D9k (S100G), are expressed in the uterine endometrium during the estrous cycle and pregnancy in a pregnancy status- and stage-specific manner, and that estrogen of conceptus origin increases endometrial TRPV6 expression. However, regulation of S100G expression in the uterine endometrium and conceptus expression of S100G has been not determined during early pregnancy. Thus, we investigated regulation of S100G expression by estrogen and interleukin-$1{\beta}$ (IL1B) in the uterine endometrium and conceptus expression of S100G during early pregnancy in pigs. We obtained uterine endometrial tissues from day (D) 12 of the estrous cycle and treated with combinations of steroid hormones, estradiol-$17{\beta}$ ($E_2$) and progesterone ($P_4$), and increasing doses of IL1B. Real-time RT-PCR analysis showed that $E_2$ and IL1B increased S100G mRNA levels in the uterine endometrium, and conceptuses expressed S100G mRNA during early pregnancy, as determined by RT-PCR analysis. To determine if endometrial expression of S100G mRNA during the implantation period was affected by the somatic cell nuclear transfer (SCNT) procedure, we compared S100G mRNA levels in the uterine endometrium from gilts with SCNT-derived conceptuses with those from gilts with conceptuses derived from natural mating on D12 of pregnancy. Real-time RT-PCR analysis showed that levels of S100G mRNA in the uterine endometrium from gilts carrying SCNT-derived conceptuses was significantly lower than those from gilts carrying conceptuses derived from natural mating. These results showed that S100G expression in the uterine endometrium was regulated by estrogen and IL1B of conceptus origin, and affected by the SCNT procedure during early pregnancy. These suggest that conceptus signals regulate S100G, an intracellular calcium transport protein, for the establishment of pregnancy in pigs.