• 한국미생물·생명공학회지
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• 제26권4호
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• pp.369-372
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• 1998

Microbial insecticide formulations were prepared by liquid and semi-solid fermentations using Bacillus thuringiensis subsp. kurstaki, HL-106 (BTK-HL106), B. thuringiensis subsp. israelensis HL-63 (BTI-HL63) and B. sphaericus 1593 (BS-1593) strains. The liquid fermentation medium contained molasses 2%, dextrose 1.5%, peptone 2%, D-xylose 0.025%, CaCl$_2$ 0.1%, K$_2$HPO$_4$ 0.1%, KH$_2$PO$_4$ 0.1%, MgSO$_4$$.$7H$_2$O 0.03%, FeSO$_4$$.$7H$_2$O 0.002%, ZnSO$_4$$.$7H$_2$O 0.02%. The composition of the semi-solid fermentation medium was rice bran 45.2%, zeolite 31%, yeast powder 0.02%, corn powder 5%, dextrose 3%, lime 0.3%, NaCl 0.06%, CaCl$_2$ 0.02%, and H$_2$O 15.42%. Insecticide formulations produced in the liquid fermentation named BTK-HL106, BTI-HL63 and BS-1593 pesticides and those in the semi-solid fermentation were designated as BTK-HL106-1, BTI-HL63-1 and BS-1593-1 pesticides, respectively. The number of spore (endotoxin crystals) was 2.65${\times}$10$\^$9/ spores per $m\ell$ in the BTK-HL106 and 3.5${\times}$10$\^$10/ in the BTK-HL106-1 3.8${\times}$10$\^$9/ spores in the BTI-HL63 and 7.0${\times}$10$\^$10/ in the BTI-HL63-1, and 7.5${\times}$10$\^$9/ in the BS-1593 and 1.4${\times}$10$\^$10/ in the BS-1593-1. The spores in the BS-1593 formulation was produced two times more than the other formulations. The spores in the BTI-HL63-1 were contained twice than those in the BTK-HL106-1, and five times than those in the BS-1593-1. The results indicated that spore (endotoxin crystals) productions in the semi-solid fermentation increased about ten times than those in the liquid fermentations. $LC_{50}$s of the BTI-HL63 and BS-1593 were 4.5 $\mu\textrm{g}$, and those of the BTI-HL63-1 and BS-1593-1 were 1.5 $\mu\textrm{g}$. $LC_{50}$ of the BTK-HL106 was 1.5 mg and that of the BTK-HL106-1 was 0.9 mg. The $LC_{50}$s of the formulations in the semi-solid fermentations showed about two to three times higher than those in the liquid fermentations.

• 한국식품저장유통학회지
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• 제10권2호
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• pp.241-245
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• 2003

기능성 발효음료를 개발하기 위하여 홍차, 감잎차, 솔잎, 쑥, 눈꽃 동충하초와 번데기 동충하초의 균사체와 자실체를 부재료로 첨가한 배지에 tea-fungus를 접종하여 3$0^{\circ}C$에서 2주일간 발효시킨 tea-fungus 음료를 조제하였다. Tea-fungus 음료의 기능성을 조사하기 위하여 음료의 원액과 그 5배 희석액으로 나누어 항균활성, 항산화능과 아질산염소거능을 조사하였다. Tea-fungus 음료의 항균활성은 솔잎과 눈꽃동충하초의 균사체와 자실체를 첨가한 경우에 E. coli와 S. aureus에 대하여 모두 우수한 항균활성을 나타내었으나 감잎차를 첨가한 음료는 항균활성을 나타내지 않았다. Tea-fungus 음료의 5배 희석액은 두 균주 모두에 대하여 항균활성을 나타내지 않았다. 음료의 전자공여능은 41-87%, 5배로 희석한 tea-fungus음료는 11-63%로서 솔잎과 감잎차를 첨가한 음료의 전자공여능이 높았고, 번데기동충하초의 균사체와 자실체를 첨가한 음료에서 낮았다. Tea-fungus 음료의 아질산염소거능홍차를 첨가한 음료에서 63%, 감잎차 44%로서 높았으며 다른 부재료를 첨가한 경우에는 전체적으로 낮은 소거능을 나타내었다.

• 한국조명전기설비학회지:조명전기설비
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• 제1권1호
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• pp.55-60
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• 1987

본 연구의 목적은 스펙트럼의 재흡수를 이용하여, 수은-아르곤 방전중의 63P 여기원자 농도를 결정하는 데 있다. 먼저, 평면거울을 사용한 경우의 재흡수 이론을 전개한 다음, 이를 수은-아르곤 방전에 적용시켜, 복잡한 미세구조를 갖는 수은의 visible-triplet lines에 있어서의 스펙트럼의 흡수계수와 측정된 흡수율사이의 수치적 관계를 구하였다. 이와함께, 흡수계수와 흡수원자농도사이의 관계를 이용하여, 방전관의 관벽온도를 변화시킨 경우와 방전전류를 변화시킨 경우에 있어서, 측정 흡수율에 상응하는 수은의 63P 여기원자 농도를 구하였고 다음과 같은 결과를 얻었다. ⅰ) 측정범위내에서 준안정원자의 농도는 63P1 상태의 농도보다 높다. ⅱ) 방전전류를 증가시킴에 따라 435.8[nm]의 재흡수가 급격히 증가하고, 그 결과 63P1 여기상태의 농도는 준안정원자에 비해 상대적으로 높은 증가속도를 보인다. ⅲ) 방전전류 증가시 준안정원자와 전자사이의 충돌이 빈번해져서 준안정원자의 증가속도가 둔화하는 대신 축적여기 또는 전리의 확률이 높아진다.

• 동의신경정신과학회지
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• 제15권1호
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• pp.127-141
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• 2004

This is 63 cases report of neurotic patient which have gone to Dep. of Neuropsychiatry of Dongshin University sunchun oriental hospital from October, 2002 to October, 2003. Objective : To find out distributional characteristics of neurotic patient Result : 1. The distribution of sex is Female and of age is thirty, fourty is large. 2. The distribution of a history of case is chronic patient is large. 3. The distributional ratio of psychosomatic : musculoskeletal : gastrointestine symptom of chief complain was 48.7: 25.3: 14.9. 4. The distribution of yanghyulansin ondam: soganhaewul gudam : bohyulansin description was 41.3: 15.9: 9.5. Conclusion : This 63 cases report is relatively small cases about analysis and comparison of neurotic patient. So, it's needed to abundant study of this, afterwards.

• Clinical and Experimental Pediatrics
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• 제63권11호
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• pp.451-453
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• 2020

• 대한의생명과학회지
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• 제27권3호
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• pp.182-186
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• 2021

Parkinson disease (PD) is becoming one of the most neurodegenerative disorder worldwide. The deposited aggregates have been connected in the pathophysiology of PD, which are degraded either by ubiquitin-proteasomal system (UPS) or autophagy-lysosomal pathway (ALP). Leucin-rich repeat kinase 2 (LRRK2), one of the neurodegenerative proteins of PD is also stringently controlled by both UPS and ALP degradation as well. However, the polyubiquitination pattern of LRRK2 aggregates is largely unknown. Here, we found that K63-linked polyubiquitinations of G2019S mutant, most familial variant for PD, is highly enhanced compared to those of wild type LRRK2 (WT). In addition, in the presence of overexpressed p62/SQSTM-1, ubiquitination of LRRK2 WT or D1994A was reduced, whereas G2019S mutant was not diminished significantly. Therefore, we propose that degradation of G2019S via UPS is more involved with K63-linked ubiquitination than K48-linked ubiquitination, and overexpressed p62/SQSTM-1 does not enhance degradative effect on G2019S variant.

• Journal of Plant Biotechnology
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• 제38권1호
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• pp.62-68
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• 2011

원예작물의 생육을 저하시키는 각종 병충해로 인한 과도한 농약과 화학비료의 사용은 환경오염뿐만 아니라 작물의 생산량에도 큰 영향을 미치고 있다. 식물생명공학기술을 이용하여 농약이나 화학비료 사용량을 획기적으로 줄일 수 있는 식물체의 개발, 즉 형질전환을 이용한 분자 육종기술은 병충해 내성 농작물을 개발하여 과도한 화학비료의 사용에 따르는 여러가지 문제점들을 극복할 수 있는 대안으로 대두되고 있다. 본 연구에서는 모델식물인 애기장대에서 분리한 식물생체방어 신호전달에 관련된 AtCBP63 유전자를 감자에 과발현시켰고, 이러한 형질 전환 감자에서 병저항성에 관여하는 유전자인 PR-2, PR-3, PR-5 유전자들의 발현이 증가되어 지속적으로 식물 방어 기작이 활성화되어 있음을 확인하였다. 또한, 감자에서 무름병 (soft rot disease)을 일으켜 막대한 피해를 유발하는 병원성 세균인 Erwinia carotovora subsp. Carotovora (Ecc)를 이용하여 AtCBP63 유전자를 과발현한 감자에 감염시켰을 때, 병 저항성이 증가한다는 사실을 검증하였다. 앞으로, 다양한 곰팡이 균에 대응하여 AtCBP63 유전자를 과발현한 감자에 저항성을 검증하고자 한다.

• 대한치과보철학회지
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• 제43권3호
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• pp.393-408
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• 2005

Statement of problem. In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. Purpose. This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). Materials and methods. MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit & acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). Results. The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. Conclusion. The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.

• Journal of Microbiology and Biotechnology
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• 제21권4호
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• pp.379-386
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• 2011

The objective of this work was to investigate the ability of the plant growth-promoting rhizobacterium Pseudomonas aureofaciens 63-28 to induce plant defense systems, including defense-related enzyme levels and expression of defense-related isoenzymes, and isoflavone production, leading to improved resistance to the phytopathogen Rhizoctonia solani AG-4 in soybean seedlings. Seven-day-old soybean seedlings were inoculated with P. aureofaciens 63-28, R. solani AG-4, or P. aureofaciens 63-28 plus R. solani AG-4 (P+R), or not inoculated (control). After 7 days of incubation, roots treated with R. solani AG-4 had obvious damping-off symptoms, but P+R-treated soybean plants had less disease development, indicating suppression of R. solani AG-4 in soybean seedlings. Superoxide dismutase (SOD) and catalase (CAT) activities of R. solani AG-4-treated roots increased by 24.6% and 54.0%, respectively, compared with control roots. Ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) activities of R. solani AG-4-treated roots were increased by 75.1% and 23.6%, respectively. Polyphenol oxidase (PPO) activity in soybean roots challenged with P. aureofaciens 63-28 and P+R increased by 25.0% and 11.6%, respectively. Mn-SOD (S1 band on gel) and Fe-SOD (S2) were strongly induced in P+R-treated roots, whereas one CAT (C1) and one APX (A3) were strongly induced in R. solani AG-4- treated roots. The total isoflavone concentration in P+Rtreated shoots was 27.2% greater than the control treatment. The isoflavone yield of R. solani AG-4-treated shoots was 60.9% less than the control.

• 한국학교ㆍ지역보건교육학회지
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• 제2권2호
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• pp.23-38
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• 2001

The purpose of this study was to suggest a stratege of promoting school health. This study examined the historical aspacts of school health and conducted a mail questionnaire survey for 24 school health specialists who work in school and educational administration from November 1 to November 30, 2000 and the reply rate was 79.2%(19 persons). The results were as follows. The most important fields in schools were answered health related field. The most important field of school health were health education(89.5%), the supervisor of school health project should be office of school health ward in Educational administration(42.1%), and problems in conducting health project in school were lack of policy(63.5%), awareness of the importance of school health(63.2%), and budget(63.2%). They answered that the cause of food poisoning in school were negligence of sanitation of cook(42.1%) and prevention methods were thorough inspection of food stuffs(31.6%). 72.2% replied that school health project were not being operated in a proper way. They answered that tasks of promoting school health were development of school health policy, increase of man-power for school health, expansion of school health budget, systematic health education, and development of independent health subject program, connection with local society. 94.7% of those replied answered that school health organization is necessary. Common sense on health and sex education are needs to be handled most importantly in health education. 63.2% of those replied answered that appropriate time of education for health service is more than once a week. The person appropriate for health education were school nurse(63.2%). In conclusion to improve the problems of school health and to activate it, development and support of policy of health project and preparation of various conditions that can establish health courses independently is, above all, immediately required. Many efforts need to be made to make the president of schools and education authorities recognize the importance of health in schools. These efforts need to link to the transformation of awareness, and process of development of concrete method of practicing various school health education and school health is necessary.