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http://dx.doi.org/10.4014/jmb.1012.12001

Effects of Pseudomonas aureofaciens 63-28 on Defense Responses in Soybean Plants Infected by Rhizoctonia solani  

Jung, Woo-Jin (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
Mabood, Fazli (Department of Plant Science, Macdonald Campus of McGill University)
Souleimanov, Alfred (Department of Plant Science, Macdonald Campus of McGill University)
Smith, Donald L. (Department of Plant Science, Macdonald Campus of McGill University)
Publication Information
Journal of Microbiology and Biotechnology / v.21, no.4, 2011 , pp. 379-386 More about this Journal
Abstract
The objective of this work was to investigate the ability of the plant growth-promoting rhizobacterium Pseudomonas aureofaciens 63-28 to induce plant defense systems, including defense-related enzyme levels and expression of defense-related isoenzymes, and isoflavone production, leading to improved resistance to the phytopathogen Rhizoctonia solani AG-4 in soybean seedlings. Seven-day-old soybean seedlings were inoculated with P. aureofaciens 63-28, R. solani AG-4, or P. aureofaciens 63-28 plus R. solani AG-4 (P+R), or not inoculated (control). After 7 days of incubation, roots treated with R. solani AG-4 had obvious damping-off symptoms, but P+R-treated soybean plants had less disease development, indicating suppression of R. solani AG-4 in soybean seedlings. Superoxide dismutase (SOD) and catalase (CAT) activities of R. solani AG-4-treated roots increased by 24.6% and 54.0%, respectively, compared with control roots. Ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) activities of R. solani AG-4-treated roots were increased by 75.1% and 23.6%, respectively. Polyphenol oxidase (PPO) activity in soybean roots challenged with P. aureofaciens 63-28 and P+R increased by 25.0% and 11.6%, respectively. Mn-SOD (S1 band on gel) and Fe-SOD (S2) were strongly induced in P+R-treated roots, whereas one CAT (C1) and one APX (A3) were strongly induced in R. solani AG-4- treated roots. The total isoflavone concentration in P+Rtreated shoots was 27.2% greater than the control treatment. The isoflavone yield of R. solani AG-4-treated shoots was 60.9% less than the control.
Keywords
Defense enzymes; isoflavones; Pseudomonas aureofaciens; Rhizoctonia solani; soybean;
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