• 한국식품과학회지
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• 제36권1호
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• pp.86-91
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• 2004

다양한 기능성 작용이 있는 물질로 알려진 OA에 대하여, 발효 과정에서 유산균에 의해 발생되는 손실을 줄이기 위한 방법을 찾고자 본 실험을 실시했다. 원유와 10% 환원유를 $121^{\circ}C$에서 15분간 열처리를 한 결과, OA의 감소율은 각자 17.6%, 22.4%, 정도로서 비교적 열에는 안정한 것으로 나타났다 발효 과정 중 유산균에 의한 OA의 변화를 측정한 결과 L. helveticus 166과 L. casei 955는 균수가 최고점에 도달하는 발효 24시간까지 OA의 감소는 미미하였으나 24시간 이후부터 72시간까지 각각 43.2%, 76%까지 OA가 급격히 감소하였고, S. thermophilus ST-37은 발효 72시간까지 OA의 감소가 미미하거나 오히려 생성하는 것으로 나타나 유산균의 종류에 따라 OA의 변화가 큰 것을 확인 할 수 있었다. 냉장 저장 중 OA함량은 유산균의 종류에 관계없이 변화가 없었다.

• 생명과학회지
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• 제11권2호
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• pp.103-110
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• 2001

This study was designed to investigate the effects of silkworm(Bombyx moril L.) powder on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10 g) were fed basic diet(control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400mg/kg BW/day for 6 weeks. There were no significant differences in cholesterol levels of brain memberanes by administration of silkworm powder (SWP). Membrane fluidities were significantly increased(21.5% and 30.8%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but significant difference between brain microsomes could not obtained. Basal oxygen radicals (BORs) in brain mitochondria and mircrosomes were significantly inhibited(8.5% and 16.5%, 16.8%and 24.8%, respectively) by SWP-200 and SEP-400 groups compared with control group. Induced oxygen radicals(IORs) in brain mitochondria were significantly inhibited(16.6% and 21.4%, respectively)by sWP-200 and SWP-400 groups compared with control group, but IOR in brain microsome were significantly inhibited about 16.0% by SWP-400 groups only compared with control group. Lipid peroxide(LPO) levels were significantly decreaed(14.8%and 22.4%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups compared with control group, but LPO level was significantly decreased about 16.0% in brain microsome of SWP-400 group only. Oxidized protein(OP) levels were remarkably decreased(about 14.8% and 16.5%, respectively) in brain mitochondria of SWP-200 and SWP-400 groups, but OP level was significantly decreased about 13.0% in brain microsome of SWP-400 group only compared with control group, Theses results suggest that administration of in brain microsome of SWP-400 group only compared with control group. These results suggest that administration of SWP may play effective role in attenuating an oxidative stress and increasing a membrane fluidity in brain membranes.

• IMMUNE NETWORK
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• 제8권2호
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• pp.53-58
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• 2008

Background: Molecular mechanisms of natural killer (NK) cell development from hematopoietic stem cells (HSCs) have not been clearly elucidated, although the roles of some genes in NK cell development have been reported previously. Thus, searching for molecules and genes related NK cell developmental stage is important to understand the molecular events of NK cell development. Methods: From our previous SAGE data-base, Gpnmb (Glycoprotein non-metastatic melanoma protein B) was selected for further analysis. We confirmed the level of mRNA and protein of Gpnmb through RT-PCR, quantitative PCR, and FACS analysis. Then we performed cell-based ELISA and FACS analysis, to know whether there are some molecules which can bind to Gpnmb. Using neutralizing antibody, we blocked the interaction between NK cells and OP9 cells, and checked IFN-${\gamma}$ production by ELISA kit. Results: Gpnmb expression was elevated during in vitro developmental stage and bound to OP9 cells, but not to NK precursor cells. In addition, we confirmed that the levels of Gpnmb were increased at NK precursor stage in vivo. We confirmed syndecan4 as a candidate of Gpnmb's binding molecule. When the interaction between NK cells and OP9 cells were inhibited in vitro, IFN-${\gamma}$ production from NK cells were reduced. Conclusion: Based on these observations, it is concluded that Gpnmb has a potential role in NK cell development from HSCs.

• 한국CDE학회논문집
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• 제13권5호
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• pp.362-371
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• 2008

Generally, a PLM education program in university consists of lectures of theory, software lab and software development raining as an advanced subject. Most industries want more than these, such as practical problem solving capabilities, teamwork skills and engineering communications including human relationship, rhetoric, technical writing, presentation and etc. Problem-Based Learning is a problem-stimulated and student-centered teaming method, and an innovative education strategy for collaborative and self-directed learning by applying real world problems. Education paradigm changes from "teaching" to "learning" accomplished by team working, and students are encouraged to develop, present, explain and defense their ideas, suggestions or solutions of a problem, and the "cooperative teaming" proceeds spontaneously during team operations. Co-operative education program is an into-grated academic model and a structured educational program combining classroom learning with productive work experience in a field related to a student's academic or career goals. Based on the partnership between academic institutions and industries, students are engaged in real and productive "work" in the industry, in contrast with merely observing. In this paper, PBL with Co-op program is suggested as an effective approach for PLM education, and we made and operated a PBL-based education course with industry co-op program. The Co-op education in industry accompanied with the PBL course in university can improve practical problem solving capabilities of students, including modeling and management of P3R(Product, Process, resource and Plant) using commercial PLM software tools. By the result, we found this to be an effective strategy for helping students, professors and industries succeed in engineering education, especially PLM area.

• 한국물환경학회지
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• 제40권2호
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• pp.67-78
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• 2024

The introduction of a significant amount of phosphorous into aquatic environments can lead to eutrophication, which can in turn result in algal blooms. For the effective management of watersheds and the prevention of water quality problems related to nonpoint organic matter (OM) sources, it is essential to pinpoint the predominant OM sources. Several potential OM sources were sampled from upper agricultural watersheds, such as fallen leaves, riparian reeds, riparian plants, paddy soil, field soil, riparian soil, cow manure, and swine manure. Stream samples were collected during two storm events, and the concentrations of dissolved organic carbon (DOC) and phosphorous (DOP) from these OM sources and stream samples were assessed. DOM indicators using fluorescence spectroscopy, including HIX, FI, BIX, and EEM-PARAFAC, were evaluated in terms of their relevance in discerning DOM sources during storm events. Representative DOM descriptors were chosen based on specific criteria, such as value ranges and pronounced differences between low and high-flow periods. Consequently, the spectral slope ratio (S_R) paired with fluorescence index (FI) using end-member mixing analysis (EMMA) proved to be suitable for estimating the contribution of organic carbon (OC). The contribution of each organic phosphorous (OP) in stream samples was determined using the phosphorous-to-carbon (P/C) ratio in conjunction with the OC contribution. Notably, OP derived from swine manure in stream samples was found to make the most dominant contribution, ranging from 61.3% to 94.2% (average 78.1% ± 12.7%). The results of this research offer valuable insights into the selection of suitable indicators to recognize various OM sources and highlight the main sources of OP in forested-agricultural watersheds.

• 산업경영시스템학회지
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• 제39권4호
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• pp.137-146
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• 2016

In recent years, business environment is faced with multi uncertainty that have not been suffered in the past. As supply chain is getting expanded and longer, the flow of information, material and production is also being complicated. It is well known that development service industry using application software has various uncertainty in random events such as supply and demand fluctuation of developer's capcity, project effective date after winning a contract, manpower cost (or revenue), subcontract cost (or purchase), and overrun due to developer's skill-level. This study intends to social contribution through attempts to optimize enterprise's goal by supply chain management platform to balance demand and supply and stochastic programming which is basically applied in order to solve uncertainty considering economical and operational risk at solution supplier. In Particular, this study emphasizes to determine allocation of internal and external manpower of developers using S&OP (Sales & Operations Planning) as monthly resource input has constraint on resource's capability that shared in industry or task. This study is to verify how Stochastic Programming such as Markowitz's MV (Mean Variance) model or 2-Stage Recourse Model is flexible and efficient than Deterministic Programming in software enterprise field by experiment with process and data from service industry which is manufacturing software and performing projects. In addition, this study is also to analysis how profit and labor input plan according to scope of uncertainty is changed based on Pareto Optimal, then lastly it is to enumerate limitation of the study extracted drawback which can be happened in real business environment and to contribute direction in future research considering another applicable methodology.

• Journal of Animal Science and Technology
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• 제46권1호
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• pp.91-96
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• 2004

유단백질에서 유래된 아미노산은 주로 L-이성체의 형태로 존재하나 일부 미생물 세포벽의 구성성분과 일부 항생물질내 D-amnio acid(DAA)가 존재하고 있는 것으로 보고 되고 있으며 이는 유제품의 품질저하와 연관된다. 따라서 이 연구는 품질저하와 연관된다. 따라서 이 연구는 내냉성미생물 P. fluorescens와 P. putida에 의한 DAA 생성에 관해 실험하였다. 상업용 혼합 유산균주인 ABT-C를 접종하여 발효시간대별로 free D-amino acid를 분석하였으며, 실험결과 전체적인 DAA 함량은 매우 낮은 수준이고, 특히 발효시간대별 변화가 크게 없어 유산균의 증식에 의한 DAA의 생성은 매우 미미한 것으로 나타났고, 또한 일부 L-amino acid의 경우 유산균의 생육에 이용되는데 DAA의 경우 발효시간대별 함량이 큰 변화가 없는 것으로 보아 유산균의 이용성 역시매우 낮은 것으로 생각된다. 원유에서 분리한 P. fluorescens와 P. putida를 접종하여 배양시간대별로 free D-amino acid를 분석하였으며 실험결과 두 처리구 모두 배양시간에 따라 DAA의 생성이 증가되는 경향을 나타내었으며, P. putida의 경우 P. fluorescens에 비해 D-Pro의 증가량이 상대적으로 높게 나타났으나 나머지 4종은 P. fluorescens에 의한 생성량이 더욱 높게 나타났고, 특히 유해성이 높은 것으로 보고된 D-Asp 및 D-Ala의 함량이 매우 높게 나타났다. 따라서 DAA는 내냉성미생물에 의한 품질저하 지표로서 이용가능성과 더불어 인체의 유해성에 대한 위험성을 배제하는 차원에서도 매우 중요하게 인식되어야 할 것으로 사료된다

• 한국식품과학회지
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• 제47권4호
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• pp.486-491
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• 2015

분유 분말의 C. sakazakii와 양파 분말의 B. cereus의 포자를 저해하는데 있어 가장 효과적인 플라즈마 형성 가스는 각각 He-$O_2$와 He이었다. 분유 분말에 대한 콜드 플라즈마 처리시간이 길어질수록 그리고 형성 전력이 높아질수록 C. sakazakii의 저해도가 커졌으며, 900W, 40분 처리시 가장 큰 저해율을 나타내었다. 양파 분말에 접종된 B. cereus 포자는 콜드 플라즈마 단독 처리와 마이크로파와 병합된 콜드 플라즈마 처리에 대하여 강한 저항력을 가지고 있었으나 열처리($90^{\circ}C$, 1분)를 두 형태의 플라즈마 처리에 선병합시켰을 때 약 90%가 저해되었다. 병합 처리한 결과, 각각의 방법을 단독으로 처리한 것보다 병합 처리시 B. cereus 포자의 저해율(약 1.0 log spores/g)이 높아졌다. 본 연구는 분체 식품에 존재하는 식중독 균의 효율적인 살균 방법을 제시하였고, 분체 식품의 새로운 살균 공정으로서의 콜드 플라즈마의 적용 가능성을 보여주었다.

• 전자통신동향분석
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• 제18권4호통권82호
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• pp.11-22
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• 2003

본 문서는 Ad-hoc 네트워크가 인터넷과 연동되는 환경에서 Mobile IP 기능을 지원하기 위한 기술에 대한 연구 동향을 분석한다. Mobile IP는 이동 노드와 Foreign Agent(FA) 간에 링크 레벨의 연결이 존재하는 네트워크 환경에서 동작하지만, Ad-hoc 네트워크의 경우 노드간에 멀티 홉 라우팅에 의한 통신 방법을 기본적으로 사용하고 있고, 노드의 자유로운 이동으로 인하여 이동 노드에서 FA로의 루트가 수시로 변경되는 특성을 가지고 있기 때문에 Ad-hoc 네트워크에서 Mobile IP를 지원하는 데에는 여러 가지 문제점이 따른다.

• 벤딩인더스트리
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• 제11권2호
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• pp.12-14
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• 2012

최근 로벤의 원두커피자판기 매출이 크게 늘었다는 소식을 들었다. 이유를 알아보니 웅진식품에서 본격적인 바바 원두커피자판기 사업 확대에 나섰다는 것이다. 그렇지만 웅진식품에 직접 원두커피자판기를 필드에 전개하는 것은 아니다. 금성경기서비스(대표;안영철)라는 업체에서 자판기 판매, 장소 섭외, 설치, A/S를 총괄한다. 웅진식품에서는 원두커피자판기를 공장도가에 판매를 하고, 한창 띄우려하는 원두커피 '바바'를 공급한다. 자판기 제조업체, 원두커피 내용물 업체, 운영업체가 공생하는 윈-윈의 사업 모델이다. 이렇게 전개 중인 바바 원두커피자판기 인기가 쏠쏠하다고 한다. 금성경기서비스의 안영철 사장을 만나 이 사업이 왜 뜨고 있는지를 알아 봤다.